BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,292,596	(C)_6→7	pseudogene (361/2531 nt)	yejO ←	autotransporter YejO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,292,590	1	.	C	95.8%	832.1 / 25.6	261	pseudogene (367/2531 nt)	yejO	autotransporter YejO
Reads supporting (aligned to +/- strand): ref base . (5/6); new base C (97/152); total (102/159)
Fisher's exact test for biased strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑CCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NZ_CP025268/2292451‑2292723

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                         >  1:645719/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                         >  2:3657595/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTATTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAAGCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                         >  2:4901240/1‑140 (MQ=255)

     gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑ccccc                                                                                                                                   <  1:4376022/140‑1 (MQ=255)

      aCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑ccccc‑c                                                                                                                                 >  1:27220/1‑140 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑ccccc‑ccatc                                                                                                                             >  1:42973/1‑136 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑ccccc‑ccatc                                                                                                                             >  1:5329423/1‑136 (MQ=255)

                  gAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCaa                                                                                                                      <  2:1099050/140‑1 (MQ=255)

                   aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAAc                                                                                                                     >  1:3032317/1‑140 (MQ=255)

                        cTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTa                                                                                                                >  1:4843249/1‑140 (MQ=255)

                           cATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CCTCTTTTTCAACGATTAAgc                                                                                                             >  2:3298721/1‑140 (MQ=255)

                           cATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAgc                                                                                                             >  1:3137344/1‑140 (MQ=255)

                             tGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAgcgc                                                                                                           >  1:2044430/1‑140 (MQ=255)

                             tGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAgcgc                                                                                                           <  1:2831871/140‑1 (MQ=255)

                             tGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAgcgc                                                                                                           >  1:4288300/1‑140 (MQ=255)

                              ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGcgcc                                                                                                          <  1:111468/140‑1 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGcgccc                                                                                                         <  1:1901245/140‑1 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGcgccc                                                                                                         <  2:5340167/140‑1 (MQ=255)

                                cccGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGcgcccg                                                                                                        >  1:1924424/1‑140 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCTATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCCACGATTAAGcgcccgcc                                                                                                      >  2:1647959/1‑140 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGcgcccgcc                                                                                                      >  1:2806263/1‑140 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGcgcccgcc                                                                                                      >  2:3261345/1‑140 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTGTAACCTCCAGTAGTCCTCCCTTTTCTATAGCGTCAAAGAC‑GGCCCCCC‑CGTCTTTTTTAAGTAATAAAagcccgcc                                                                                                      >  2:2571095/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCGTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  1:4465210/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  1:4140500/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  2:3191483/1‑140 (MQ=255)

                                     gTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCttt                                                                                                   <  2:182439/140‑1 (MQ=255)

                                         ttgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATc                                                                                               >  1:3887893/1‑140 (MQ=255)

                                          tgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                              <  2:1764176/140‑1 (MQ=255)

                                          tgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                              <  1:4030904/140‑1 (MQ=255)

                                            ttttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            <  1:3455119/140‑1 (MQ=255)

                                                 aTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATc                                                                                       >  1:4821270/1‑140 (MQ=255)

                                                   aGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTCTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCAt                                                                                     >  1:2175811/1‑140 (MQ=255)

                                                     cACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc                                                                                   >  1:2590971/1‑140 (MQ=255)

                                                      cccGCCTGCTTTCTGACCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCg                                                                                  <  1:2967549/139‑1 (MQ=255)

                                                       ccGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGc                                                                                 >  2:2307006/1‑140 (MQ=255)

                                                       ccGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGc                                                                                 >  2:342659/1‑140 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  1:1174123/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  1:4546193/140‑1 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:1588312/1‑140 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:3736829/1‑140 (MQ=255)

                                                            tGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgcgc                                                                            <  1:2221817/140‑1 (MQ=255)

                                                              ctgtCTGATCTACCGTATATGCCAGTCCCCCCTCTTTCACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GT‑CCCCCTCATCTTTTTCAACGATTCAGCGCCCGCCTTTAATCGTGGGATCATTCGATgcgcga                                                                          <  2:2688798/137‑3 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGGATCATTCGCTGCGCCa                                                                          <  1:538464/140‑1 (MQ=255)

                                                               tttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTACCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt                                                                         <  2:3838408/140‑1 (MQ=255)

                                                                 tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                                                                       <  2:645657/140‑1 (MQ=255)

                                                                 tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                                                                       <  1:337260/140‑1 (MQ=255)

                                                                        tACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTa                                                                >  1:869552/1‑140 (MQ=255)

                                                                         cccGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  2:1896994/139‑1 (MQ=255)

                                                                         aCCTCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  2:3155079/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  2:4540669/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:3679110/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:1816144/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:3076727/140‑1 (MQ=255)

                                                                           cGCAAATGCAACTCCCCCCTCCTTAACCCCCAGCAGTCCTCACTTTTCCATAGCGACAAAGAC‑GGCCCCCC‑CCTCTTTTTCAACGCTTACGCGCACGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:1352092/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCGCCCCTCTTTAACCTCCAGTAGTCCTCCCTTGTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCACAAACCTGTAGca                                                             <  2:5283413/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTCGca                                                             <  1:2369126/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:5045386/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:2135878/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:2249903/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:1420269/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:1235601/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:2501537/140‑1 (MQ=255)

                                                                             caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca                                                           <  2:2442725/140‑1 (MQ=255)

                                                                              acatgcacatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAATAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCacac                                                          <  2:2935919/138‑1 (MQ=255)

                                                                              aaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCacac                                                          <  2:2044430/140‑1 (MQ=255)

                                                                                atgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                        <  1:2211499/140‑1 (MQ=255)

                                                                                     aatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattatt                                                   <  2:4821270/140‑1 (MQ=255)

                                                                                     aatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattatt                                                   <  2:4140500/140‑1 (MQ=255)

                                                                                          ccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCg                                              <  1:360136/140‑1 (MQ=255)

                                                                                           cccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGt                                             >  1:3858348/1‑140 (MQ=255)

                                                                                              tctTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAga                                          >  1:4727868/1‑139 (MQ=255)

                                                                                                  tAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTTTAGCACACCATTATTTAGCGTAGTCTgg                                      >  2:5015146/1‑140 (MQ=255)

                                                                                                        ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                                >  2:814017/1‑140 (MQ=255)

                                                                                                        ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                                >  1:3693018/1‑140 (MQ=255)

                                                                                                        ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGGAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                                >  2:965916/1‑140 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               <  1:3221022/140‑1 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               <  1:4585789/140‑1 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  2:1407302/1‑140 (MQ=255)

                                                                                                              gttctccCTTTTCTATCGCCCCTATGACTGG‑CCCCC‑CATCTTTTTCAACGAGTAAGCGCCCGTCTTTAATCGTGGTATCATTCGCTGCGCCATTAACCTCTAGCAGACCATTATTGAGCGTAGTCTGGTGTGCcgcgtga                          <  2:1591213/137‑4 (MQ=255)

                                                                                                              gtCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                          <  1:1440927/140‑1 (MQ=255)

                                                                                                               tcctccCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAc                         >  1:2920627/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:5123334/102‑1 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  1:2096380/1‑140 (MQ=255)

                                                                                                                   cccTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt                     >  2:4839851/1‑140 (MQ=255)

                                                                                                                   cccTTTTCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt                     >  2:407091/1‑140 (MQ=255)

                                                                                                                        ttCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGaa                >  1:1396755/1‑140 (MQ=255)

                                                                                                                         tCGATAGCGACAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc               <  1:1274313/140‑1 (MQ=255)

                                                                                                                                 aaCAAAGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCGATTAACCTGTAGCACACCATTCTTGAGCGTAGTCTGGTTTCCCGCGCCACCCATTTGAACAATTTGAt       <  2:3048500/139‑1 (MQ=255)

                                                                                                                                    aaaGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAAc    <  1:5166712/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGTCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:355005/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:864727/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:905434/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:1468119/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:3388082/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:4328626/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:3657595/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:2435806/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:2760619/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTAGTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:4863099/140‑1 (MQ=255)

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑CCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NZ_CP025268/2292451‑2292723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`