BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,466,923	(C)_8→9	intergenic (+242/‑123)	fadL → / → CXP41_RS12955	long‑chain fatty acid transporter FadL/protein YfdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,466,915	1	.	C	93.5%	431.0 / 22.9	139	intergenic (+234/‑131)	fadL/CXP41_RS12955	long‑chain fatty acid transporter FadL/protein YfdF
Reads supporting (aligned to +/- strand): ref base . (1/8); new base C (18/112); total (19/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

TTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑CCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCA  >  NZ_CP025268/2466776‑2467046

ttttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACt                                                                                                                                         >  2:4404339/1‑140 (MQ=255)

  ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cc                                                                                                                                      >  1:4355395/1‑140 (MQ=255)

  ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cc                                                                                                                                      >  2:2504974/1‑140 (MQ=255)

  ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cc                                                                                                                                      >  1:2203363/1‑140 (MQ=255)

  ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTAGCCACATACAAACCTGAAGGGAAAGGCATATTTTGAGGCGGTCTGCACGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATGTCCAGGAT‑ACT‑ca                                                                                                                                      >  2:4635286/1‑139 (MQ=255)

   tGCAACCACCCTGTTGGAAGCTTCACCTTTCAAATCCCTAAGCGGTATCCACAGACAAACCTGAACGGAACGGCACATTTTCAGGCCATCCGCTCGTCCTTCTCAACGACTTTCC‑TTTTCTGCATTTCCAGGCT‑ACT‑ccc                                                                                                                                     <  2:4057429/140‑1 (MQ=255)

   tGCAAACAGCCTTTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAATGCGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑CTTTCTGCAGTTCCAGGAT‑ACT‑ccc                                                                                                                                     <  2:562218/140‑1 (MQ=255)

   tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑ccc                                                                                                                                     <  1:721277/140‑1 (MQ=255)

   tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑ccc                                                                                                                                     <  1:778287/140‑1 (MQ=255)

   tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑ccc                                                                                                                                     <  1:2153185/140‑1 (MQ=255)

     cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cccc‑c                                                                                                                                  <  1:2087020/140‑1 (MQ=255)

       aaCAGGCTGTTGGCACCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCCCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCCGGAT‑ACT‑cccc‑c‑cc                                                                                                                               <  1:2161429/140‑1 (MQ=255)

       aaCAGCCTGTTGGCAGCTTGCCCTTTCACATCAATCAGTGGTATCCACAGACAAACCTGAAGGTAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cccc‑c‑cc                                                                                                                               <  2:1146097/140‑1 (MQ=255)

       aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGCAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cccc‑ctc                                                                                                                                <  1:2976816/140‑3 (MQ=255)

       aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cccc‑c‑cc                                                                                                                               <  1:4009920/140‑1 (MQ=255)

       aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cccc‑c‑cc                                                                                                                               <  1:3241985/140‑1 (MQ=255)

       aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cccc‑c‑cc                                                                                                                               <  2:2512574/140‑1 (MQ=255)

       aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cccc‑c‑cc                                                                                                                               <  2:3345222/140‑1 (MQ=255)

       aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAACCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cccc‑c‑cc                                                                                                                               <  2:5113786/140‑1 (MQ=255)

       aaCAGCCTGTTGGCAGCTTGCCATTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGTCGTTCTGCTCGTCCTTCTCAAAGAGTTTCC‑TTTTCTGCATTTCCAGGAT‑ACT‑cccc‑c‑cc                                                                                                                               <  2:2865058/140‑1 (MQ=255)

         cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cccc‑c‑cc‑cc                                                                                                                            <  1:4547151/140‑2 (MQ=255)

         cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTAGCCACAGACAAACCGGAAGGGAAAGGCATCTTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cccc‑c‑cc‑cc                                                                                                                            <  2:4954150/140‑2 (MQ=255)

            ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑cccc‑c‑cc‑cctgg                                                                                                                         <  1:4584279/140‑5 (MQ=255)

                  ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCttttt                                                                                                                    >  1:1428629/1‑139 (MQ=255)

                    cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATtgtg                                                                                                                  <  2:1175461/140‑1 (MQ=255)

                    cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATtgtg                                                                                                                  <  1:2720539/140‑1 (MQ=255)

                    cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATtgtg                                                                                                                  <  1:5080239/140‑1 (MQ=255)

                    cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATtgtg                                                                                                                  <  1:3116946/140‑1 (MQ=255)

                    cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATtgtg                                                                                                                  <  1:1032704/140‑1 (MQ=255)

                      gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTgcg                                                                                                                <  2:1349579/140‑1 (MQ=255)

                      gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTgcg                                                                                                                <  1:1011052/140‑1 (MQ=255)

                      gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTgcg                                                                                                                <  1:3553040/140‑1 (MQ=255)

                      gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTgcg                                                                                                                >  1:3614859/1‑140 (MQ=255)

                         tGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTc                                                                                                             >  1:1974528/1‑140 (MQ=255)

                         tGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTc                                                                                                             >  1:1988293/1‑140 (MQ=255)

                           cccTTTCAAATCAATAAGCGGTATCCACAGACAACCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCAt                                                                                                           <  1:582149/140‑1 (MQ=255)

                           cccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCAt                                                                                                           <  1:5290236/140‑1 (MQ=255)

                            ccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATa                                                                                                          <  2:1356088/140‑1 (MQ=255)

                            ccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATa                                                                                                          <  2:1128556/140‑1 (MQ=255)

                               ttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATacac                                                                                                       <  1:538035/140‑1 (MQ=255)

                                tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACt                                                                                                      <  2:1441494/140‑1 (MQ=255)

                                tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACCCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACt                                                                                                      <  2:764871/140‑1 (MQ=255)

                                 cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTc                                                                                                     <  1:3820/140‑1 (MQ=255)

                                    aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACGCTCaaa                                                                                                  <  1:1561522/140‑1 (MQ=255)

                                    aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCaaa                                                                                                  <  1:2126052/140‑1 (MQ=255)

                                    aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCaaa                                                                                                  <  1:3050812/140‑1 (MQ=255)

                                            ttggTAGCCACAGACAAAACTGATGGGAAAGGCATATTGTCCGGCGTTCTGCTCGTCCTTCTCAAATAGTTTAC‑TTTTCTGCATTTCCAGAAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          <  2:5119081/138‑1 (MQ=255)

                                            gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          <  1:1631256/140‑1 (MQ=255)

                                            gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          <  2:2552919/140‑1 (MQ=255)

                                            gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          <  2:3954974/140‑1 (MQ=255)

                                            gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          <  1:1265144/140‑1 (MQ=255)

                                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                     <  2:2235294/140‑1 (MQ=255)

                                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                     <  2:2020483/140‑1 (MQ=255)

                                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                     <  1:4044583/140‑1 (MQ=255)

                                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                     <  1:2710988/140‑1 (MQ=255)

                                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                     <  2:4681769/140‑1 (MQ=255)

                                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAGAGGtt                                                                                     <  1:1441221/140‑1 (MQ=255)

                                                   ccACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCTAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAGATTAAAGATAGGTTCt                                                                                   >  1:2965440/1‑140 (MQ=255)

                                                   ccACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCt                                                                                   <  2:3614859/140‑1 (MQ=255)

                                                              ccTTAAGGGAAAGCATTCTTTTCAGGCGTTCTGCTCTTCCTTCTCAAAGCGTTTAC‑TTTTCTGCATTCCAGGGAT‑ACCCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                        <  2:1358078/140‑1 (MQ=255)

                                                                tGAAGGGAAAGGCATATTTTCAGGCGTTCTGCCCGTCCTTCTCAAAGAGTTTAC‑TTTTCTCCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCGATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCg                                                                      <  1:4890094/140‑1 (MQ=255)

                                                                   aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttt                                                                   >  1:1114934/1‑140 (MQ=255)

                                                                     ggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑CCCC‑CACC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttttt                                                                 <  1:1532692/140‑1 (MQ=255)

                                                                       aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                                                               <  1:4774185/140‑1 (MQ=255)

                                                                        aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  1:821672/140‑1 (MQ=255)

                                                                        aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  2:3695274/140‑1 (MQ=255)

                                                                        aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  2:4518519/140‑1 (MQ=255)

                                                                         aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                                                             >  1:2664591/1‑140 (MQ=255)

                                                                         aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                                                             >  1:2018875/1‑140 (MQ=255)

                                                                          ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                            <  2:162186/140‑1 (MQ=255)

                                                                          gccATAATTTCAGTCGTTCTTCTCGTCATTCACAAAGAGTTTAC‑TTCTCTCCCCTTCCAACAT‑ACT‑CCCC‑C‑CCACTGGCTATTGTGCGCTCATACACACAAACTAAAGCTAGGTTCTAAATAAATGCGCGTCTTTTGATa                                                            <  2:4597721/138‑1 (MQ=255)

                                                                           gCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                                                           <  1:4600131/140‑1 (MQ=255)

                                                                            cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                                          <  1:1101524/140‑1 (MQ=255)

                                                                            cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                                          <  1:1007650/140‑1 (MQ=255)

                                                                               tttttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                       <  1:3157214/139‑1 (MQ=255)

                                                                                    cAGGCGTTCTGCCCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACCCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                  <  1:988106/140‑1 (MQ=255)

                                                                                     aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCACGAT‑ACT‑CCCCTA‑CC‑CTGGCTATTGTGCGCTCCTACACTCAAATTAAAGATCGGTTCTACATACATGAGCGTTTTTTGATAGTCTCTTTCAt                                                 <  2:2056886/140‑1 (MQ=255)

                                                                                      ggCGTTCTGCTCGTCCTTCTCAAAGANTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                <  2:1882127/140‑1 (MQ=255)

                                                                                      ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                <  1:3732260/140‑1 (MQ=255)

                                                                                      ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                <  1:4289381/140‑1 (MQ=255)

                                                                                      ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                <  1:2540250/140‑1 (MQ=255)

                                                                                      ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATGTCAtt                                                <  1:2826395/140‑1 (MQ=255)

                                                                                      ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATGTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCATATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                <  1:3204418/140‑1 (MQ=255)

                                                                                         gTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                             <  2:4746957/140‑1 (MQ=255)

                                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGt                                            >  1:3498404/1‑140 (MQ=255)

                                                                                           tctcCTAGTCCTCCCCAAAG‑GTTTACTTTTTCACAATTTCCAGAATCCCC‑CCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                           <  2:1428629/140‑1 (MQ=255)

                                                                                           tCTGCTTGTCGTTCTCAAAGCGTTTAC‑TTTTCTGCATTTCCAGGAT‑CCTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCGAGTTAATGATAGGTTCTAAATAATTGAGCGTTTTTTGATGGTCTATTTCATTAGGTa                                           <  2:5250200/140‑1 (MQ=255)

                                                                                           tCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                           <  1:2504974/140‑1 (MQ=255)

                                                                                            cTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTaa                                          <  2:637114/140‑1 (MQ=255)

                                                                                            cTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑CCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAat                                         >  1:4687334/1‑140 (MQ=255)

                                                                                                   tCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatatatat                                   <  2:2728118/140‑1 (MQ=255)

                                                                                                                   tAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaatc                   >  2:1642526/1‑140 (MQ=255)

                                                                                                                   tAC‑TTTTCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaatc                   >  2:1643256/1‑140 (MQ=255)

                                                                                                                        tttCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa               <  2:2375817/140‑1 (MQ=255)

                                                                                                                        tttCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa               <  2:845043/140‑1 (MQ=255)

                                                                                                                        tttCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa               <  2:922708/140‑1 (MQ=255)

                                                                                                                        tttCTGCATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATAAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa               <  1:4416199/140‑1 (MQ=255)

                                                                                                                              cATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAAt         <  2:4304025/140‑1 (MQ=255)

                                                                                                                              cATTTCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCGATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAAt         <  2:2965440/140‑1 (MQ=255)

                                                                                                                                  tCCAGGAT‑ACTCCCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATaaaa     <  1:2417636/140‑1 (MQ=255)

TTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAC‑TTTTCTGCATTTCCAGGAT‑ACT‑CCCC‑C‑CC‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCA  >  NZ_CP025268/2466776‑2467046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`