BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,786,415	+C	pseudogene (334/4580 nt)	ypjA ←	adhesin‑like autotransporter YpjA/EhaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,786,411	1	.	C	97.6%	1098.4 / 16.0	334	pseudogene (338/4580 nt)	ypjA	adhesin‑like autotransporter YpjA/EhaD
Reads supporting (aligned to +/- strand): ref base . (2/6); new base C (140/185); total (142/192)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

GGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑CC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACA  >  NZ_CP025268/2786272‑2786545

ggCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑a                                                                                                                                          <  1:4450707/140‑1 (MQ=255)

 gcaaccaccAACAATGAGTATTTCATTCGCACTCCCTCCCTCTACGCGTAAAGCCCGGCCATGAATTGTCGTTCTAGCGCTGATACCCCCTTTATAAACGTCCTGATTGCGACTGTCGATTGTCGTACGAGTTGAAAT‑A‑c                                                                                                                                        >  2:4341662/4‑140 (MQ=255)

 gCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑c                                                                                                                                        >  1:2933443/1‑140 (MQ=255)

  cTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGGCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑cc                                                                                                                                       >  1:2359795/1‑140 (MQ=255)

   tACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑cc‑c                                                                                                                                     <  1:324498/140‑1 (MQ=255)

   tACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑cc‑c                                                                                                                                     <  1:2235854/140‑1 (MQ=255)

       accaTCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑cc‑cccgt                                                                                                                                 <  1:3306061/140‑4 (MQ=255)

           tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATg                                                                                                                              >  1:360671/1‑140 (MQ=255)

               tGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAAtt                                                                                                                          >  2:1234183/1‑140 (MQ=255)

                gAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTg                                                                                                                         >  1:437277/1‑140 (MQ=255)

                 aGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGa                                                                                                                        <  2:255511/140‑1 (MQ=255)

                 aGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGa                                                                                                                        <  1:569155/140‑1 (MQ=255)

                   tATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACt                                                                                                                      >  2:33156/1‑140 (MQ=255)

                     ttCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGt                                                                                                                    >  1:5129055/1‑140 (MQ=255)

                     ttCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGt                                                                                                                    >  1:377508/1‑140 (MQ=255)

                      tCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTc                                                                                                                   >  1:1124733/1‑140 (MQ=255)

                         aTTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGc                                                                                                                <  2:5035627/140‑1 (MQ=255)

                          ttCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGcc                                                                                                               >  2:1547895/1‑140 (MQ=255)

                           tCGCCCTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAACCGTCCTGATTGCCCCTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCa                                                                                                              <  1:4586265/140‑1 (MQ=255)

                             gCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑cc‑cccgt                                                                                                                                 >  2:1374054/1‑115 (MQ=255)

                                ctccctccCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGtt                                                                                                         <  1:2593701/140‑1 (MQ=255)

                                 tccctcccTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTa                                                                                                        <  2:3057080/140‑1 (MQ=255)

                                  ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                       <  1:414815/140‑1 (MQ=255)

                                   cctccctcTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAAt                                                                                                      >  1:2604719/1‑140 (MQ=255)

                                       cctcTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGtt                                                                                                  <  2:3792938/140‑1 (MQ=255)

                                        ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                                 <  1:1415160/140‑1 (MQ=255)

                                        ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                                 <  1:4424001/140‑1 (MQ=255)

                                        ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                                 <  2:2817494/140‑1 (MQ=255)

                                         tctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGt                                                                                                >  2:3864827/1‑140 (MQ=255)

                                           tACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtat                                                                                              >  2:3069324/1‑140 (MQ=255)

                                            aCGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatt                                                                                             >  1:1271613/1‑140 (MQ=255)

                                             cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                            >  2:1125055/1‑140 (MQ=255)

                                             cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                            >  1:2021246/1‑140 (MQ=255)

                                             cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                            <  2:581174/140‑1 (MQ=255)

                                             cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                            >  1:1460557/1‑140 (MQ=255)

                                             cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                            <  1:481499/140‑1 (MQ=255)

                                               cgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTattatt                                                                                          >  1:2684565/1‑140 (MQ=255)

                                               cgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTattatt                                                                                          >  1:956614/1‑140 (MQ=255)

                                               cgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTattatt                                                                                          >  2:707706/1‑140 (MQ=255)

                                                 tGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAg                                                                                        >  1:636731/1‑140 (MQ=255)

                                                   aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                      >  2:1560879/1‑140 (MQ=255)

                                                   aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                      >  1:649238/1‑140 (MQ=255)

                                                   aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                      >  2:4936911/1‑140 (MQ=255)

                                                     gCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTg                                                                                    <  1:1068553/140‑1 (MQ=255)

                                                         cGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTTCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGcccc                                                                                >  1:2136673/1‑140 (MQ=255)

                                                              ttAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGGCCCCCaaa                                                                           >  2:1998087/1‑140 (MQ=255)

                                                                     tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACTTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACAGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                    <  2:5170245/140‑1 (MQ=255)

                                                                     tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                    >  1:4694680/1‑140 (MQ=255)

                                                                     tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                    >  1:2797390/1‑140 (MQ=255)

                                                                     tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                    >  1:3485272/1‑140 (MQ=255)

                                                                     tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                    <  2:3479039/140‑1 (MQ=255)

                                                                      cGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt                                                                   <  1:747965/140‑1 (MQ=255)

                                                                       gTTCCATTGCTGATACCCCCTTTATACACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCAGCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATg                                                                  <  2:2536179/140‑1 (MQ=255)

                                                                       gTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATg                                                                  <  2:4230371/140‑1 (MQ=255)

                                                                        ttCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa                                                                 <  2:2193086/140‑1 (MQ=255)

                                                                        ttCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTAACTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTCTTAGCCTGCCCCACAAAATTGTTATGa                                                                 <  2:3927493/140‑1 (MQ=255)

                                                                         tCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                                <  1:298676/140‑1 (MQ=255)

                                                                         tCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                                <  2:4612849/140‑1 (MQ=255)

                                                                         tCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                                <  2:3593372/140‑1 (MQ=255)

                                                                         tCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                                <  1:1336901/140‑1 (MQ=255)

                                                                          ccACTGCTGATACCCCCTTTATAAACGTCCTCATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACt                                                               <  2:3167213/140‑1 (MQ=255)

                                                                            aTTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTc                                                             <  2:4556445/140‑1 (MQ=255)

                                                                             ttGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTcc                                                            >  2:3504884/1‑140 (MQ=255)

                                                                                cTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCtat                                                         <  2:4523330/140‑1 (MQ=255)

                                                                                    tACCCCCTTTATAAACCTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCt                                                     <  1:4356567/140‑1 (MQ=255)

                                                                                        cccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGat                                                 <  2:1748458/140‑1 (MQ=255)

                                                                                        cccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGat                                                 <  2:3431027/140‑1 (MQ=255)

                                                                                         ccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATCC‑CC‑CCGTCATGAATTGACTGTCTGCCAACGTTACTGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGata                                                <  2:2376636/140‑1 (MQ=255)

                                                                                         ccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGata                                                <  2:4838182/140‑1 (MQ=255)

                                                                                          cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGatat                                               >  1:2450713/1‑140 (MQ=255)

                                                                                          cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGatat                                               >  2:1663657/1‑140 (MQ=255)

                                                                                             tagtgccGTCCTGCTTGTCTCTCTCGATTGGTGCACCTGTTGCAAT‑ACCC‑CCGTCCTGAATGGACTGTCTGCCACCGTTATTGGTTGTATTATTAGCCTGCCCCACAAAGTTGTTATGACTTCCTATATCTTGATATcca                                            <  2:923155/134‑1 (MQ=255)

                                                                                              ataAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATccac                                           <  1:1904619/140‑1 (MQ=255)

                                                                                                  aCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTTACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                       <  2:4015641/140‑1 (MQ=255)

                                                                                                  aCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                       <  1:3861912/140‑1 (MQ=255)

                                                                                                  aCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                       <  1:4559253/140‑1 (MQ=255)

                                                                                                  aCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                       <  1:4936911/140‑1 (MQ=255)

                                                                                                    gTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGTTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTTATATCCACCAGAtt                                     >  1:4059363/1‑140 (MQ=255)

                                                                                                     tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTc                                    >  1:3155450/1‑140 (MQ=255)

                                                                                                     tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTc                                    >  2:3667393/1‑140 (MQ=255)

                                                                                                     tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATGGTTATGACTTCCTATATCTTGATATCCACCAGATTc                                    >  2:1813100/1‑140 (MQ=255)

                                                                                                       cTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCaa                                  >  2:4452620/1‑140 (MQ=255)

                                                                                                          aTTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATaa                               >  1:3985374/1‑140 (MQ=255)

                                                                                                             gCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                            <  1:4981886/140‑1 (MQ=255)

                                                                                                             gCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                            <  1:1723299/140‑1 (MQ=255)

                                                                                                             gCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                            <  2:2258332/140‑1 (MQ=255)

                                                                                                              ccACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACt                           >  2:1036571/1‑140 (MQ=255)

                                                                                                               cACTCTCGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACtt                          <  2:2689480/140‑1 (MQ=255)

                                                                                                                  tctcGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCa                       <  1:5189819/140‑1 (MQ=255)

                                                                                                                  tctcGATTGTCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCa                       >  1:2487599/1‑140 (MQ=255)

                                                                                                                         tGTCGTCCCTGTGGAAAT‑A‑CCACCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATa                <  1:875109/140‑1 (MQ=255)

                                                                                                                           tCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATAcc              <  2:247469/140‑1 (MQ=255)

                                                                                                                           tCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATAcc              <  1:1701374/140‑1 (MQ=255)

                                                                                                                           tCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATAcc              <  1:1526979/140‑1 (MQ=255)

                                                                                                                           tCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATAcc              <  2:150051/140‑1 (MQ=255)

                                                                                                                           tCGTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTCCCATTAGATAcc              <  2:5284188/140‑1 (MQ=255)

                                                                                                                             gTACCTGTGGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATAcccc            <  1:1234183/140‑1 (MQ=255)

                                                                                                                                  tgtgGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCCCCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCAt       <  1:5275616/140‑1 (MQ=255)

                                                                                                                                  tgtgGAAAT‑ACCC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCAt       <  2:3335616/140‑1 (MQ=255)

                                                                                                                                  tgtgGAAAT‑ACCC‑CCGGCATGAATTGAGTGTCTACGACCGTTAAAGGTTGTATTATTAGCCTAGCTCACAAAATTGTTATGACTTCCGATATGGTGATATCCACCAGATGAAATAAGACATGCATTGGATACACCGCCAt       >  2:84094/1‑140 (MQ=255)

GGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑CC‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACA  >  NZ_CP025268/2786272‑2786545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`