BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,858,752	+T	pseudogene (395/2078 nt)	flhA →	formate hydrogenlyase transcriptional activator FlhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,858,748	1	.	T	96.7%	1026.3 / 19.7	273	pseudogene (391/2078 nt)	flhA	formate hydrogenlyase transcriptional activator FlhA
Reads supporting (aligned to +/- strand): ref base . (7/2); new base T (153/111); total (160/113)
Fisher's exact test for biased strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 333 total aligned reads displayed.

ACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑T‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCG  >  NZ_CP025268/2858609‑2858882

aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑Cata                                                                                                                                            >  1:968805/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTTGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  2:1676199/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  2:3947127/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  2:2750047/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  1:689515/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  1:533132/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  1:3435230/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  1:206544/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  1:1805607/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  1:1700261/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  2:2261055/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  2:195744/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑g‑t‑t‑t                                                                                                                                    >  2:1859554/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGGGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  2:4717865/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCACCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑t                                                                                                                                    >  1:4245609/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCGGATACGCTGCATTGCAGTTACGAAGAATGTTTTGAAACCTGGCCGCAGCTGGACGCAGGGGGGCTATACCCAAAATTTTGTCACTATTGCCTGATGCCACTGGCGGCGTAATGG‑CATA‑t‑tgg                                                                                                                                      >  2:4156127/1‑138 (MQ=255)

      caTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑ttg                                                                                                                                  >  1:1062834/1‑138 (MQ=255)

      caTTTTGCCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCCGCTGGACGCAGGTGGGCTCTACCCAAAATTTGGTCACTATTGACTGATGCCACTGGCGCCGGAAGGG‑CATA‑t‑t‑t‑ttg                                                                                                                                  <  1:2322952/140‑3 (MQ=255)

       aTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑ttgg                                                                                                                                 <  1:1867130/140‑4 (MQ=255)

       aTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑t‑t‑t‑ttgg                                                                                                                                 <  1:4831933/140‑4 (MQ=255)

              cgcgCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCtgt                                                                                                                           >  1:4604576/1‑140 (MQ=255)

              cgcgCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCtgt                                                                                                                           <  2:1360564/140‑1 (MQ=255)

                cgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGa                                                                                                                         >  1:506235/1‑140 (MQ=255)

                cgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGa                                                                                                                         >  1:3781739/1‑140 (MQ=255)

                cgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTCGGTCACTATTGCCTGATGCCACTGGCGGCGGAGGGG‑CAAATT‑T‑T‑TGGTGGCtgtta                                                                                                                         >  1:2511485/1‑138 (MQ=255)

                  cccTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAAt                                                                                                                       >  1:4188832/1‑140 (MQ=255)

                     tGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTa                                                                                                                    >  1:4848481/1‑140 (MQ=255)

                     tGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTa                                                                                                                    <  1:3981928/140‑1 (MQ=255)

                       aTACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTAtt                                                                                                                  >  1:2882067/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:2679212/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:3561779/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:3327384/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:1497294/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  2:888696/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  2:2102274/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:4461524/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  2:4505833/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACATGGCCGCAGCTGGGCGCGGGTGGGCTACACCCAAAATTTGGTCACTATGGCCTGATGCCACTGCGGGCGGCAGGGACTCT‑T‑T‑T‑TGGTGGCTGTGAATTTCTTCggtgtgg                                                                                                          >  2:4891070/1‑134 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCGTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:195744/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:4940778/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:2025451/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:3265613/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:4674507/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:3166311/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:754599/140‑1 (MQ=255)

                                    cAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:4386386/139‑1 (MQ=255)

                                    cAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      >  2:4386386/1‑139 (MQ=255)

                                     ggTTACGAAGAAGTGTGGGAGACCTGGCCGCGGGTGGACGCAGGGGGGCTATACCCAAAATTTGGTCACGATGGCCTGATGCCACGGGCGGGGGACGGG‑CATA‑T‑T‑TGTGGTGGCTGTGAATTGAGTCGTTAGGACgcgcg                                                                                                    <  2:1295216/139‑5 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  2:1773616/1‑140 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  2:259907/1‑140 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  2:3859401/1‑140 (MQ=255)

                                                   ttGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaa                                                                                      <  2:3446329/140‑1 (MQ=255)

                                                   ttGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaa                                                                                      <  2:2229656/140‑1 (MQ=255)

                                                               gCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATTACGATCGCCCCTGGAGCGAAAAAGAGGTCAATc                                                                          >  2:2449279/1‑140 (MQ=255)

                                                                ccGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTTGCTGTGAATTTATTCGGTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCg                                                                         >  2:2977320/1‑140 (MQ=255)

                                                                    aGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGTGTTCAATCGTCTg                                                                     >  1:4703918/1‑140 (MQ=255)

                                                                     gCTGGACGCAGGTGGGCTATACCCAAAAGTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTGTGc                                                                    >  2:760807/1‑140 (MQ=255)

                                                                      cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa                                                                   <  2:1282076/140‑1 (MQ=255)

                                                                      cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa                                                                   <  2:5221417/140‑1 (MQ=255)

                                                                        ggACGCGGGTGGGCTATACGCAAAATTTGTGCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TTGTGGCTGTGAATTGATTCGTGATGACGATTGCCTCTGGAGCGAAAAAGAATTCATACGTCTGCaaa                                                                 >  2:1384541/1‑140 (MQ=255)

                                                                          aCGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACa                                                               <  2:4525207/140‑1 (MQ=255)

                                                                              aGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTa                                                           >  1:4885774/1‑140 (MQ=255)

                                                                              aGGTGGGCTATACCCAAAATTTGGTCACTATGGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCGGGAGCGAAAAAGAGGTCAATCGTCTGCAAACATTTa                                                           >  2:2851670/1‑140 (MQ=255)

                                                                                gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         <  2:482527/140‑1 (MQ=255)

                                                                                gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         >  1:932530/1‑140 (MQ=255)

                                                                                gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         >  1:1671546/1‑140 (MQ=255)

                                                                                gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         <  1:364533/140‑1 (MQ=255)

                                                                                gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         >  1:4484908/1‑140 (MQ=255)

                                                                                     cTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGAt                                                    <  2:5071895/140‑1 (MQ=255)

                                                                                        taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑T‑TCT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCTCCCGGTGCGAAAAAGATCGCAATCGTCGGCAAACATGTACGCAGATCGt                                                 >  2:3630201/1‑140 (MQ=255)

                                                                                          cccAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGttt                                               >  2:533702/1‑140 (MQ=255)

                                                                                           ccAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTc                                              >  1:1600383/1‑140 (MQ=255)

                                                                                              aaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTTCAAACATTTACGCAGATCGTTTCTgg                                           >  2:2796138/1‑139 (MQ=255)

                                                                                              aaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgt                                           >  1:1418237/1‑140 (MQ=255)

                                                                                                 tttGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgtcgt                                        <  1:3072515/140‑1 (MQ=255)

                                                                                                  ttGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCTCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGGTTCTgtcgtt                                       >  2:3699524/1‑139 (MQ=255)

                                                                                                     gTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAcc                                    >  2:1456395/1‑140 (MQ=255)

                                                                                                     gTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAcc                                    >  1:117091/1‑140 (MQ=255)

                                                                                                         ctcttGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  2:405418/137‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGGAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTCCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  2:4207233/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  1:2370649/140‑1 (MQ=255)

                                                                                                                   aTGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCagag                      >  2:348284/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:3112845/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:900926/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:5279105/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:3127204/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:5181139/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:3411182/1‑140 (MQ=255)

                                                                                                                      ccACTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcg                   <  1:606685/140‑1 (MQ=255)

                                                                                                                       cACTGGAGGAAGAAGTG‑CATATT‑T‑T‑TGGTGGTTGTGTATTTATTCCTTATGACGTTCGCAACTGGAGCGGAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTGCTGTCGTCACCGAACAAATCCAGAGCcgc                  <  2:1100439/140‑1 (MQ=255)

                                                                                                                        aCTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgcg                 >  2:5148020/1‑140 (MQ=255)

                                                                                                                         cTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgt                >  1:5228558/1‑140 (MQ=255)

                                                                                                                         cTGGCGGCGGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgt                >  1:2250909/1‑140 (MQ=255)

                                                                                                                            gcggcgGAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgtcgt             >  1:3496961/1‑140 (MQ=255)

                                                                                                                              ggcggAAGGG‑CATA‑TCT‑T‑TGGAGGCTGTGACTTTATTCGTTATGACGATCGTCCCTGGGGCGAAAAAGAGTTCAATCGACTGCAAACATTTACGCAGATCGATTCAGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTa           >  2:2408950/1‑140 (MQ=255)

                                                                                                                               gcggAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTaa          <  1:1887247/140‑1 (MQ=255)

                                                                                                                                cggAAGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAAc         >  2:1598960/1‑140 (MQ=255)

                                                                                                                                    aGGG‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATg     <  1:5139164/140‑1 (MQ=255)

                                                                                                                                     ggg‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGt    <  1:1328338/140‑1 (MQ=255)

                                                                                                                                       g‑CATATT‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCg  <  2:3935809/140‑1 (MQ=255)

ACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGG‑CATA‑T‑T‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCG  >  NZ_CP025268/2858609‑2858882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`