BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,014,858	+G	intergenic (+159/+61)	arcC → / ← yqeB	carbamate kinase/selenium‑dependent molybdenum cofactor biosynthesis protein YqeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,014,857	1	.	G	98.8%	1112.8 / 4.0	339	intergenic (+158/+62)	arcC/yqeB	carbamate kinase/selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
Reads supporting (aligned to +/- strand): ref base . (2/2); new base G (167/166); total (169/170)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 353 total aligned reads displayed.

ATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑A‑G‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTACG  >  NZ_CP025268/3014719‑3014991

 tACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑A‑gg                                                                                                                                       >  1:5081751/1‑139 (MQ=255)

   ccATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑A‑ggca                                                                                                                                     >  1:4986849/1‑137 (MQ=255)

   ccATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑A‑ggca                                                                                                                                     >  2:568159/1‑137 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAAtt                                                                                                                                  >  2:495869/1‑140 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAAtt                                                                                                                                  >  2:5194143/1‑140 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAAtt                                                                                                                                  >  1:3727701/1‑140 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAAtt                                                                                                                                  >  1:4612728/1‑140 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAAtt                                                                                                                                  >  2:3373216/1‑140 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAAtt                                                                                                                                  >  2:757669/1‑140 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAAtt                                                                                                                                  >  2:3629825/1‑140 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTACTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAAtt                                                                                                                                  >  2:4765153/1‑140 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCATCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAAtt                                                                                                                                  >  1:119241/1‑140 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTGGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTTCAATTCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAAtt                                                                                                                                  >  2:4326113/1‑140 (MQ=25)

       ccGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTCGGCCTGATAAGCGTAGCGCATC‑A‑gggagttt                                                                                                                                 >  1:4864655/1‑133 (MQ=255)

            cAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGt                                                                                                                            <  1:256478/140‑1 (MQ=255)

            cAAAGAGTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGAGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCACATTCACTAGATTGCAATGCACTTGTAGGCCTGATAAGCCTAGCGCATC‑AGG‑CAATTTGGCGt                                                                                                                            <  2:2806243/140‑1 (MQ=25)

                   tGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGtcat                                                                                                                     <  2:1082799/140‑1 (MQ=255)

                   tGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGtcat                                                                                                                     <  1:564349/140‑1 (MQ=255)

                   tGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGtcat                                                                                                                     <  2:1516678/140‑1 (MQ=255)

                   tGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGtcat                                                                                                                     <  2:2075295/140‑1 (MQ=255)

                         gtaagtGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGtct                                                                                                               <  1:593678/140‑1 (MQ=255)

                              tGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATGCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGc                                                                                                          <  2:4707077/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑GAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:4067941/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:817693/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:4406581/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:39507/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:1761675/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:1936568/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:3981651/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:2153734/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:2433112/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:3582012/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:2280648/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑AAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:2867947/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGCAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCATTCTCAGGCCgg                                                                                                       <  2:3799984/140‑1 (MQ=255)

                                  cTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCggg                                                                                                      >  1:3838443/1‑140 (MQ=255)

                                   tttGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCAGCAGTCTCAGGCCgggt                                                                                                     >  1:4358731/1‑140 (MQ=255)

                                           tGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATa                                                                                             >  2:3585771/1‑140 (MQ=255)

                                            gCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATAc                                                                                            >  1:3228556/1‑140 (MQ=255)

                                                      gcgcGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCa                                                                                  >  2:2168619/1‑140 (MQ=255)

                                                       cgcgAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCAt                                                                                 >  2:3418529/1‑140 (MQ=255)

                                                        gcgAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCAtt                                                                                <  1:150285/140‑1 (MQ=255)

                                                         cgAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCAttt                                                                               <  1:1000720/140‑1 (MQ=255)

                                                               ccTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCAttt                                                                         <  1:1237776/140‑1 (MQ=255)

                                                                  tATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAg                                                                      <  2:4829248/140‑1 (MQ=255)

                                                                   aTCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGc                                                                     <  1:86495/140‑1 (MQ=255)

                                                                    tCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTACGcc                                                                    <  1:3061016/140‑1 (MQ=255)

                                                                    tCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGcc                                                                    <  2:1561950/140‑1 (MQ=255)

                                                                        tCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACtt                                                                >  2:3208885/1‑140 (MQ=255)

                                                                        tCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACtt                                                                >  1:1693461/1‑140 (MQ=255)

                                                                        tCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTAGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACtt                                                                >  1:4441285/1‑140 (MQ=255)

                                                                         ccTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTc                                                               >  2:3712463/1‑140 (MQ=255)

                                                                         ccTACAAAACATCGCAAATTCAATAGACTGCAATGCACTTGTAGTCCTGATAAGCGTAGCGCATC‑AGG‑CCATCTGGCGTTTGTCATCAGTCTCCGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTc                                                               <  2:585568/140‑1 (MQ=37)

                                                                          cTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTccc                                                              >  2:420659/1‑140 (MQ=255)

                                                                          cTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTcc                                                              >  1:1789499/1‑140 (MQ=255)

                                                                          cTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTcc                                                              >  1:4558107/1‑140 (MQ=255)

                                                                             cAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGc                                                           <  2:119241/140‑1 (MQ=255)

                                                                                  cATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCActtc                                                      >  2:4486688/1‑140 (MQ=255)

                                                                                  cATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCActtc                                                      >  1:3995569/1‑140 (MQ=255)

                                                                                   aTCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAgg                                                                                                           >  2:5295076/1‑86 (MQ=255)

                                                                                     cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCttt                                                   >  1:4022578/1‑140 (MQ=255)

                                                                                     cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCttt                                                   >  1:2220916/1‑140 (MQ=255)

                                                                                     cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCttt                                                   >  1:3317419/1‑140 (MQ=255)

                                                                                     cGCAAATTCAATAGATTGCAATGCAATTGTAGGGCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTGGTCATCTGTCTCAGGCGGGGGGGGTAATGCGATCCGGCCATTTTCAGTTAAGCGACTCCCAGCACTTCgtt                                                   >  2:2932862/1‑140 (MQ=21)

                                                                                          aTTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCg                                              <  2:2267274/140‑1 (MQ=255)

                                                                                          aTTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCg                                              <  2:1342002/140‑1 (MQ=255)

                                                                                              aaTAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCttt                                          <  1:3373216/140‑1 (MQ=255)

                                                                                                tAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑A‑GTCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCa                                        <  1:3037741/140‑1 (MQ=255)

                                                                                                  gATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACg                                      <  1:2003917/140‑1 (MQ=255)

                                                                                                  gATTGCAATGAACTTGTCGGTCTGATAAGCGTAGCGCATCAA‑T‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATACGGCCATTTTCAGTGAAGCCGCTTCCCGGACTTCTATTGTCGCTTACACg                                      <  2:3547859/140‑1 (MQ=25)

                                                                                                    ttGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGGTTGTCATCAGTCTCAGGCCGGGTGGGTAATAACATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCGTTCACGcc                                    <  2:2340810/140‑1 (MQ=38)

                                                                                                     tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       >  2:3244302/1‑72 (MQ=255)

                                                                                                     tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc                                   >  1:2509075/1‑140 (MQ=255)

                                                                                                     tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc                                   >  1:4809657/1‑140 (MQ=255)

                                                                                                      gCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCt                                  <  2:2721136/140‑1 (MQ=255)

                                                                                                              cTTGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATc                          <  2:4585853/140‑1 (MQ=255)

                                                                                                               ttGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCTTTTGTCATCAGTCCCAGGCCGTGTGGGTAATCCCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCa                         <  2:1245551/140‑1 (MQ=38)

                                                                                                                tGTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCaa                        <  2:2428901/140‑1 (MQ=255)

                                                                                                                 gTAGGCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAc                       >  2:2035233/1‑140 (MQ=255)

                                                                                                                    ggCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAcatc                    >  1:918883/1‑140 (MQ=255)

                                                                                                                    ggCCTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCGCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAcatc                    >  2:1640411/1‑140 (MQ=255)

                                                                                                                      ccTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATCTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAcatcat                  >  1:3800775/1‑140 (MQ=255)

                                                                                                                       cTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCAtt                 <  1:1080195/140‑1 (MQ=255)

                                                                                                                       cTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCAtt                 <  2:3063513/140‑1 (MQ=255)

                                                                                                                       cTGATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCAtt                 <  2:186846/140‑1 (MQ=255)

                                                                                                                         gATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTag               >  1:4731898/1‑139 (MQ=255)

                                                                                                                         gATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               >  1:1391803/1‑140 (MQ=255)

                                                                                                                         gATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               <  1:1537964/140‑1 (MQ=255)

                                                                                                                         gATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               >  1:266179/1‑140 (MQ=255)

                                                                                                                         gATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               >  1:3412323/1‑140 (MQ=255)

                                                                                                                         gATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               >  1:4177121/1‑140 (MQ=255)

                                                                                                                         gATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               >  1:5147721/1‑140 (MQ=255)

                                                                                                                         gATAAGCGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               >  1:998881/1‑140 (MQ=255)

                                                                                                                          aTAAGCGTCGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGCTGCATCAACATCATTAAc              >  1:691222/1‑140 (MQ=255)

                                                                                                                               cGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTc         <  1:5256793/140‑1 (MQ=255)

                                                                                                                               cGTAGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTc         >  2:5031414/1‑140 (MQ=255)

                                                                                                                               cGTAGCGCATC‑AGG‑CAATTTGGCGTTTGCCATCAGTCTCAGGCCGGGTGGGTAATACCATCGGGCCATTTTCATTTAAGCCACTTCCAGCCCTTCTTTTGTCGCTTTCACGCCCTGATGCATTAACATCCTTAACGCCTc         >  1:4926029/1‑140 (MQ=255)

                                                                                                                                  aGCGCATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAg      >  2:3860429/1‑140 (MQ=255)

                                                                                                                                   gcgcATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGt     <  2:2769696/140‑1 (MQ=255)

                                                                                                                                    cgcATC‑AGG‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTa    >  1:1553787/1‑140 (MQ=255)

ATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATC‑A‑G‑CAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTACG  >  NZ_CP025268/3014719‑3014991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`