BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,016,563	+C	intergenic (‑19/+28)	yqeB ← / ← yqeC	selenium‑dependent molybdenum cofactor biosynthesis protein YqeB/selenium cofactor biosynthesis protein YqeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,016,562	1	.	C	96.5%	918.6 / 23.4	285	intergenic (‑18/+29)	yqeB/yqeC	selenium‑dependent molybdenum cofactor biosynthesis protein YqeB/selenium cofactor biosynthesis protein YqeC
Reads supporting (aligned to +/- strand): ref base . (4/6); new base C (90/185); total (94/191)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

TCGGCGCGCACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑T‑C‑TTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCA  >  NZ_CP025268/3016423‑3016696

tCGGCGCGCACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGCAAATATTCATACCAACCTCAATGGat‑a‑t                                                                                                                                          >  1:1278549/1‑140 (MQ=255)

tCGGCGCGCACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGat‑a‑t                                                                                                                                          >  2:3801392/1‑140 (MQ=255)

tCGGCGCGCACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGat‑a‑t                                                                                                                                          >  1:4183399/1‑140 (MQ=255)

tCGGCGCGCACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGat‑a‑t                                                                                                                                          >  1:4703684/1‑140 (MQ=255)

tCGGCGCGCACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGat‑a‑t                                                                                                                                          >  2:667442/1‑140 (MQ=255)

     gcgcACTAACTTTTGTGCAGAATGGCGGGTAGTCGAGCCTCGGCTATCAACAGTTGGCGCCATAGCAAGCGGACAATGTTGCTCTTCGAGTTTTGCAGCCTCTGTGCAAATATTCATGCCAACCTCAATGGAT‑A‑T‑C‑cttt                                                                                                                                   <  2:2613195/140‑2 (MQ=255)

     gcgcACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATTAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATGCATACCAACCTCAATGGAT‑A‑T‑C‑cttt                                                                                                                                   >  2:4201467/1‑139 (MQ=255)

     gcgcACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑T‑ccttt                                                                                                                                    <  1:3379960/140‑5 (MQ=255)

      cgcACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑T‑cctttc                                                                                                                                   >  1:4800595/1‑135 (MQ=255)

       gcACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCa                                                                                                                                  <  1:5099377/140‑1 (MQ=255)

        cACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAg                                                                                                                                 <  1:4398464/140‑1 (MQ=255)

            aaaaTTTGTGCAGAATGGCGGGGAGCCGACCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAAc                                                                                                                             <  2:2165411/137‑1 (MQ=255)

            aaCATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAAc                                                                                                                             <  1:2215453/140‑1 (MQ=255)

            aaCATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAAc                                                                                                                             <  2:3848903/140‑1 (MQ=255)

                   gtgCAGGATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAAt                                                                                                                      >  1:4080913/1‑140 (MQ=255)

                   gtgCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTCACCCGTAAt                                                                                                                      >  2:3725564/1‑140 (MQ=255)

                   gtgCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAAt                                                                                                                      >  1:1033836/1‑140 (MQ=255)

                   gtgCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAAt                                                                                                                      >  2:2350147/1‑140 (MQ=255)

                     gCAGAATGGCGGGGAGTCGAGCCTCGGCTCTCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAACTATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATAc                                                                                                                    >  2:1843967/1‑140 (MQ=255)

                         aaTGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCgg                                                                                                                <  2:1233120/140‑1 (MQ=255)

                           tGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCTAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑T‑CGCTTCAGTAACCCGGAATACCCGGGc                                                                                                              >  2:1868295/1‑140 (MQ=255)

                           tGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGc                                                                                                              >  1:635784/1‑140 (MQ=255)

                           tGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGc                                                                                                              <  1:755625/140‑1 (MQ=255)

                              cGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCgc                                                                                                           >  1:4326735/1‑139 (MQ=255)

                                gggAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCgcta                                                                                                         <  1:4503081/140‑4 (MQ=255)

                                gggAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTa                                                                                                         <  2:1290920/140‑1 (MQ=255)

                                gggAGTCGAGCCCCGGCTATCAACAATTTGCGCCATCGCAACCGGACCATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATGCATACCAACCTCAATGGAT‑ACC‑C‑TTTCAGTAACCCGGAATACCCGGGCCGCTa                                                                                                         <  2:602793/140‑1 (MQ=255)

                                gagAGTCGCGCCGCGGCTATCAACAAGTTGCGCCATCGCCAACGGACAACTTTGCTCTTCGAGTTTTGCAGCCTCTGTGACAATATTCATACCAGCCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGCAATACCCGGGCCgcaa                                                                                                         <  2:3662126/138‑4 (MQ=255)

                                  gggTCGAGCCTTGGATATCAACAATTTGCGCCATAGCAAACGGACTATGTTGCTCTTCGAGTTTTGCAGCCTTTGTGAAAATATTCATCCCAACCCCAATGGAT‑A‑TCC‑TTTCAGGGTCCCGGAATACCCGGGCCGCTAAt                                                                                                       <  2:196924/138‑1 (MQ=255)

                                  gAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAAt                                                                                                       <  2:4166545/140‑1 (MQ=255)

                                  gAGCCGAGCTTCGGTGATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAAACCGGAATACCCGGGCCGCTAAt                                                                                                       <  1:2746620/140‑1 (MQ=255)

                                   aGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAAtt                                                                                                      >  2:3049563/1‑140 (MQ=255)

                                   aGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAAtt                                                                                                      <  2:1642847/140‑1 (MQ=255)

                                     tCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTca                                                                                                    >  2:1136187/1‑140 (MQ=255)

                                       gAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTcaca                                                                                                  <  2:2071437/140‑1 (MQ=255)

                                               gCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcg                                                                                          >  1:1480776/1‑140 (MQ=255)

                                               gCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcg                                                                                          <  1:3007969/140‑1 (MQ=255)

                                               gCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcg                                                                                          <  1:3082723/140‑1 (MQ=255)

                                                 tctCACCAATCTGCGCCATCGCAAACGGCCAATTTTGCTCTTCGAGTTTTACAGCCTCTGTGAAAATCTTCATACCCCACTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcgcg                                                                                        <  1:841641/138‑1 (MQ=255)

                                                 tctCAACCATTTGCGCAATAGCAAAAGGACAATTTTGCTCCTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCGCAATGAAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAGTCTccgag                                                                                        <  2:3191316/138‑3 (MQ=255)

                                                 tATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcgcg                                                                                        <  2:2917918/140‑1 (MQ=255)

                                                 tATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcgcg                                                                                        <  1:4963331/140‑1 (MQ=255)

                                                    caacaaTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCGCTGTGAAAATATTCATACCAACCGCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGa                                                                                     <  1:2294360/140‑1 (MQ=255)

                                                        aaTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGc                                                                                 >  2:3768592/1‑140 (MQ=255)

                                                         aTTTGCGCCATAGCAAACGGACCATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCa                                                                                <  2:7157/140‑1 (MQ=255)

                                                          tttGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTTACAAATCTTCGCGCTATTGCAg                                                                               >  2:803500/1‑140 (MQ=255)

                                                          tttGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAg                                                                               >  2:1490545/1‑140 (MQ=255)

                                                          tttGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAg                                                                               <  1:2383657/140‑1 (MQ=255)

                                                          tttGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAg                                                                               >  2:4044058/1‑140 (MQ=255)

                                                          tttGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAg                                                                               >  1:136220/1‑140 (MQ=255)

                                                           ttGCGAAATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TGTCAGTAACCCGGAATACCCGTGCCGCTAATTCACAAATCTGCGCGCGATTGCAgg                                                                              <  1:2129524/140‑1 (MQ=255)

                                                            tGCGCCATAGCAAACGGACAATTTTTCTCTTCGAGTTTTGCAGCCTCTGTGATAATATTCATACCACCATCAATGGGT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAACTCTGCGCGCGATTGCAGGa                                                                             <  1:3925886/140‑1 (MQ=255)

                                                            tGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                             <  1:667442/140‑1 (MQ=255)

                                                            tGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                             <  1:2892269/140‑1 (MQ=255)

                                                              cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                           <  1:3587433/140‑1 (MQ=255)

                                                              cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                           <  2:4584550/140‑1 (MQ=255)

                                                              cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                           <  1:2332689/140‑1 (MQ=255)

                                                              cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                           <  1:2794575/140‑1 (MQ=255)

                                                              cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                           <  1:4470608/140‑1 (MQ=255)

                                                              cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGAGATTGCAGGATg                                                                           <  2:5293338/140‑1 (MQ=255)

                                                                 cATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTc                                                                        <  2:4963621/140‑1 (MQ=255)

                                                                 cATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGCGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTc                                                                        <  1:4621683/140‑1 (MQ=255)

                                                                  aTAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCt                                                                       <  1:1097652/140‑1 (MQ=255)

                                                                  aTAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCt                                                                       <  1:3223873/140‑1 (MQ=255)

                                                                   tAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCtt                                                                      <  1:3838542/140‑1 (MQ=255)

                                                                       aaaCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTACCCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGtat                                                                  <  1:4762825/140‑1 (MQ=255)

                                                                       aaaCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGtat                                                                  <  2:1055721/140‑1 (MQ=255)

                                                                           ggACAGTTTCGCTCGTTGAGGTCTACAGCCTCGGTGAAGATATTCATCCCAACGTCGATGGAT‑A‑T‑CTTTTGAGTCACCCGGGATACCCGGGCCCCTAATTCGCAAATCGCCGCGCGATTGCAGGATGTTCTTGTATATCg                                                              <  2:4906186/140‑1 (MQ=255)

                                                                             aCAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGcc                                                            >  2:2139369/1‑140 (MQ=255)

                                                                             aCAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGcc                                                            <  1:184375/140‑1 (MQ=255)

                                                                             aCAATTTTGCTCTTCGAGTGTTGGAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCCCAAATCTGCTCGCGATTGCAGGATGTTCTTGTATATCGcc                                                            <  1:2586369/140‑1 (MQ=255)

                                                                              cAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGccc                                                           >  1:3765623/1‑140 (MQ=255)

                                                                                      ctctTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                   <  1:2076638/140‑1 (MQ=255)

                                                                                      ctctTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                   <  2:3521131/140‑1 (MQ=255)

                                                                                      ctctTCGAGTTTTGCAACCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                   <  1:4352296/140‑1 (MQ=255)

                                                                                       tctTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAtt                                                  <  1:3112136/140‑1 (MQ=255)

                                                                                           cGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCct                                              <  2:899776/140‑1 (MQ=255)

                                                                                             aGTTTTGCAGCCTCTGTGAAAATATTCATACGAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCctct                                            <  2:1014408/140‑1 (MQ=255)

                                                                                                tttGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCCCCAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcg                                         <  2:5144620/140‑1 (MQ=255)

                                                                                                   gCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtcg                                      >  2:2435580/1‑140 (MQ=255)

                                                                                                   gCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtcg                                      <  1:3474137/140‑1 (MQ=255)

                                                                                                   gCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTAcgtcg                                      <  2:1659384/140‑1 (MQ=255)

                                                                                                     aGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTATTGTATATCGCCCAGCCAGATTGCCTCTACGTCgtg                                    <  1:5233024/140‑1 (MQ=255)

                                                                                                             tgAAAATCATCATACCAACCTCAGTGGAT‑C‑T‑CATTCCGGTAACCCGCCTTCCCCGGTCCGCTAATTCACGAATCTGCCCGCGATTGCAGGATGTTCTTGTATATCACCCAGCCAGATTCAATCTACGTCGTGATGTTGCa                            <  2:3115773/140‑1 (MQ=255)

                                                                                                             tgAAAATATTCATGCCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCCGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa                            <  2:4633883/140‑1 (MQ=255)

                                                                                                             tgAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa                            <  2:2475882/140‑1 (MQ=255)

                                                                                                             tgAAAATATTCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa                            <  1:5072119/140‑1 (MQ=255)

                                                                                                             tcaaaaGATACCTACCAACCTCAATGGAT‑A‑TCC‑TATCAGTACCCCGGTACACCCGGCCCGCTCATTCCCAAATCTGCTTGCATTTGCAGTATGTTCTAGTATCTCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa                            <  2:5221361/138‑1 (MQ=255)

                                                                                                                     ttCATACCAACCTCAATGGATAA‑C‑C‑TTTCAGTAACCCGGAATACCCGGTCCGATAATTCACAAATCTGCGCGCTATTGCATGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGa                    >  1:1593857/1‑140 (MQ=255)

                                                                                                                     ttCATACCAACCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACCAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGa                    >  1:1762227/1‑140 (MQ=255)

                                                                                                                             aaCCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTc            >  2:4808894/1‑140 (MQ=255)

                                                                                                                              aCCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg           >  1:1678886/1‑140 (MQ=255)

                                                                                                                              aCCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg           >  1:897009/1‑140 (MQ=255)

                                                                                                                              aCCTCAATGGAT‑A‑TCC‑TTTCAGTAACCCCGGATACCAGGGCCGCTAATTCACACATCTGCGAGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGACTGCCTCTACGTTGTGTTGTTTCAGCGGTGGAAGGAGCttt           >  2:394553/1‑138 (MQ=255)

                                                                                                                               ccTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGc          >  2:1370929/1‑140 (MQ=255)

                                                                                                                                cTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCt         <  1:560544/140‑1 (MQ=255)

                                                                                                                                cTCAATGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCt         <  2:277630/140‑1 (MQ=255)

                                                                                                                                   aaTGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGCTTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTtg      >  2:2825471/1‑140 (MQ=255)

                                                                                                                                   aaTGGAT‑A‑TCC‑TTGCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTtg      >  2:4521183/1‑140 (MQ=255)

                                                                                                                                     tGGAT‑A‑TCC‑TTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGACTCTCCGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTtgtg    <  1:1536286/140‑1 (MQ=255)

TCGGCGCGCACTAACATTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT‑A‑T‑C‑TTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCA  >  NZ_CP025268/3016423‑3016696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`