BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	320,615	(G)_6→7	intergenic (+482/‑44)	rclR → / → ykgE	reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	320,609	1	.	G	97.3%	729.5 / 10.4	220	intergenic (+476/‑50)	rclR/ykgE	reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster
Reads supporting (aligned to +/- strand): ref base . (6/0); new base G (109/105); total (115/105)
Fisher's exact test for biased strand distribution p-value = 3.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

AGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGT‑‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NZ_CP025268/320470‑320743

aGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGt                                                                                                                                            >  2:2706476/1‑140 (MQ=255)

 gCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGT‑‑g                                                                                                                                         >  1:764306/1‑140 (MQ=255)

  cGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGT‑‑g‑g                                                                                                                                       <  1:491187/140‑1 (MQ=255)

  cGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGT‑‑g‑g                                                                                                                                       >  2:2239551/1‑140 (MQ=255)

    aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGT‑‑g‑ggg                                                                                                                                     >  2:836576/1‑140 (MQ=255)

    aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGT‑‑g‑ggg                                                                                                                                     >  1:4248282/1‑140 (MQ=255)

    aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGT‑‑g‑ggg                                                                                                                                     >  1:237877/1‑140 (MQ=255)

     tGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGT‑‑g‑gggg                                                                                                                                    >  1:2299849/1‑140 (MQ=255)

      gCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGT‑‑g‑gggg‑g                                                                                                                                  >  1:58757/1‑140 (MQ=255)

            agaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCCTACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACa                                                                                                                             <  2:626144/140‑1 (MQ=255)

            agaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACa                                                                                                                             <  2:1870659/140‑1 (MQ=255)

            agaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACa                                                                                                                             <  1:5077330/140‑1 (MQ=255)

              aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATc                                                                                                                           <  1:103796/140‑1 (MQ=255)

               aaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCg                                                                                                                          >  2:4005681/1‑140 (MQ=255)

                  aaaaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGc                                                                                                                       <  1:2751142/140‑1 (MQ=255)

                   aaaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCa                                                                                                                      <  1:153177/140‑1 (MQ=255)

                       tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCCTATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATaaa                                                                                                                  <  1:199730/140‑1 (MQ=255)

                           tATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATggg                                                                                                              <  2:3061599/140‑1 (MQ=255)

                            aTTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAGATGGGc                                                                                                             >  2:4975534/1‑140 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCa                                                                                                            <  1:2106981/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCa                                                                                                            <  1:4351431/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCa                                                                                                            <  2:2206332/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCa                                                                                                            <  2:2863503/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCa                                                                                                            <  2:418170/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGCTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCa                                                                                                            <  2:4329020/140‑1 (MQ=255)

                              tGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATGTTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCAt                                                                                                           >  2:760031/1‑140 (MQ=255)

                               gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATg                                                                                                          <  2:4152941/140‑1 (MQ=255)

                                 aTTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGaa                                                                                                        <  2:1954584/140‑1 (MQ=255)

                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  1:1463090/140‑1 (MQ=255)

                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    >  1:1434674/1‑140 (MQ=255)

                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  1:4009414/140‑1 (MQ=255)

                                     gACGTTTGGATGAAAGATTATCATTTGGCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAATCATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  2:5114658/140‑1 (MQ=255)

                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  2:2605272/140‑1 (MQ=255)

                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  2:4679613/140‑1 (MQ=255)

                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  1:2677184/140‑1 (MQ=255)

                                          ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAg                                                                                               >  2:15700/1‑140 (MQ=255)

                                          ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAg                                                                                               >  1:1558116/1‑140 (MQ=255)

                                           tGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGGAGTAATGGAGt                                                                                              >  1:5070231/1‑140 (MQ=255)

                                            ggATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTa                                                                                             <  2:3737479/140‑1 (MQ=255)

                                              aTGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAtt                                                                                           <  1:3555422/140‑1 (MQ=255)

                                                  aaGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGtt                                                                                       >  2:1151360/1‑140 (MQ=255)

                                                              tGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAt                                                                           >  1:5338718/1‑140 (MQ=255)

                                                               gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAGTGGAGTATTAGTTGTGAATGTCAAtt                                                                          >  2:274369/1‑140 (MQ=255)

                                                               gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                                                                          <  1:3990538/140‑1 (MQ=255)

                                                                   tACAAATGAGGGGTGGTCTGTTGCTAGCAAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCtt                                                                      >  2:1361687/1‑140 (MQ=255)

                                                                    aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     <  2:338569/140‑1 (MQ=255)

                                                                      aaaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                   <  2:1662154/140‑1 (MQ=255)

                                                                      aaaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                   <  2:571054/140‑1 (MQ=255)

                                                                       aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc                                                                  <  2:2402899/140‑1 (MQ=255)

                                                                        aTGAGGGGAGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGGTGTGAATGTCAATTTCTTTGTCa                                                                 >  1:995981/1‑140 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:4660198/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:3651646/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:4025773/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:1151360/140‑1 (MQ=255)

                                                                          gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc                                                               <  2:3014997/140‑1 (MQ=255)

                                                                           tggggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCt                                                              <  1:4709610/139‑1 (MQ=255)

                                                                             gggTGGGATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGG‑‑GTGGGT‑GAAACATCGGGCATAAATGGGCATGAAATAATGGAGTATTAGTTGTGAATGTAAATTTCTTTATCACCTGt                                                            >  2:4056260/1‑140 (MQ=255)

                                                                              ggtggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 >  1:2816332/1‑102 (MQ=255)

                                                                              ggtggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  2:2816332/102‑1 (MQ=255)

                                                                                       ttGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACg                                                  >  1:817388/1‑140 (MQ=255)

                                                                                       ttGCCAGGCAATTAAAAAAGAACGGCATATTTTTTGATTATTTATTCCCC‑GGGGGG‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGAc                                                   <  2:4417513/140‑1 (MQ=255)

                                                                                        tGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGc                                                 >  1:836814/1‑140 (MQ=255)

                                                                                        tGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCAGAAATGGGCATGAAGTAATGGAGTATTAGTTGGGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGc                                                 >  1:5133481/1‑140 (MQ=255)

                                                                                         gCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGTATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGcc                                                <  1:4788847/140‑1 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATTCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGcgc                                               >  1:3750558/1‑138 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACGTCGGGCATATATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  2:4919532/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 >  2:2618270/1‑90 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  1:2618270/90‑1 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGCATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:5275364/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:4452400/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  2:183897/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:1739866/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:2498495/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  2:1474323/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTGATGGATGAGTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAGGTAATGGAGTATTAGTTGTGAATGTCAATGTCTTTGTCACCTGTATTGGTGACGccg                                               >  2:3128702/1‑139 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACTCCAGATTGATTGATGATTGATCCCCCGGGG‑‑G‑GGGG‑GAAACAGCGGGCATAAATGGGCCTGAAAAAATGGAGGAGTAGTTGTGAATGTCAATTTCTTAGTCAACTATATTGGTGACGccgtta                                            >  2:4839409/1‑136 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  2:4454266/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  1:1650493/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  1:5782/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  2:315469/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  1:1911606/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  2:2990080/1‑140 (MQ=255)

                                                                                                 aTTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATc                                        >  2:1019681/1‑140 (MQ=255)

                                                                                                  ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa                                       >  1:1079231/1‑140 (MQ=255)

                                                                                                  ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa                                       >  1:3242827/1‑140 (MQ=255)

                                                                                                      aaaaGAACGCCATATTTATTGATGATTGATCCCCC‑GGAG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   <  2:4921057/140‑1 (MQ=255)

                                                                                                            aCGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCAc                             >  1:3665265/1‑140 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGGAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            <  2:5070231/140‑1 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            >  1:2594102/1‑140 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            >  1:3000076/1‑140 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            <  1:4618033/140‑1 (MQ=255)

                                                                                                                  tatttattGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACt                       <  2:685243/140‑1 (MQ=255)

                                                                                                                       attGATGATTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtg                  >  1:2029916/1‑140 (MQ=255)

                                                                                                                          gatgatTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtgctg               >  1:3421562/1‑140 (MQ=255)

                                                                                                                          gatgatTGATCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCAGGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtgctg               >  2:4210853/1‑140 (MQ=255)

                                                                                                                              attgatCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  1:4927515/1‑140 (MQ=255)

                                                                                                                                  atCCCCC‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGa       >  1:3154504/1‑140 (MQ=255)

                                                                                                                                      ccc‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGaaaaa   >  1:5101061/1‑140 (MQ=255)

                                                                                                                                       cc‑GGTG‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAc  >  2:363400/1‑140 (MQ=255)

                                                                                                                                       cc‑GGT‑‑G‑GGGGTGAAACATCTGGCATAAATTGGCATGAGGTAATGGAGTATTAGGTGTGAATGTCAATTTCTTTGTCACTTTTATTGGTGTCGCCCTGAAATCAAGAATTGCACGAGACTCCGTGCTTCTACTAGAAAAAc  >  2:4560663/1‑140 (MQ=255)

AGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCC‑GGT‑‑G‑GGGG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NZ_CP025268/320470‑320743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`