BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,542,898	(A)_6→7	pseudogene (27/602 nt)	fimB →	type 1 fimbria switch DNA invertase FimB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,542,892	1	.	A	97.7%	654.6 / 5.4	174	pseudogene (21/602 nt)	fimB	type 1 fimbria switch DNA invertase FimB
Reads supporting (aligned to +/- strand): ref base . (4/0); new base A (110/59); total (114/60)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

CCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑AAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGT  >  NZ_CP025268/4542754‑4543026

ccACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑a                                                                                                                                         >  1:1552559/1‑140 (MQ=255)

        gttagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑aaa‑a‑aaagg                                                                                                                               >  1:1292658/1‑137 (MQ=255)

         ttagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑aaa‑a‑aaagga                                                                                                                              <  2:2474714/140‑5 (MQ=255)

          tagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑aaa‑a‑aaaggaa                                                                                                                             <  2:4011859/140‑6 (MQ=255)

          tagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑aaa‑a‑aaaggaa                                                                                                                             <  1:2817976/140‑6 (MQ=255)

          tagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑aaa‑a‑aaaggaa                                                                                                                             <  1:5347332/140‑6 (MQ=255)

           agttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAAc                                                                                                                             >  2:3353110/1‑140 (MQ=255)

           agttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAAc                                                                                                                             >  1:2648329/1‑140 (MQ=255)

             ttattaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACtt                                                                                                                           >  2:1871134/1‑140 (MQ=255)

                 taAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTg                                                                                                                       >  1:391611/1‑140 (MQ=255)

                 taAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTg                                                                                                                       >  1:773602/1‑140 (MQ=255)

                         cGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAg                                                                                                               <  2:1504520/140‑1 (MQ=255)

                          gTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGt                                                                                                              >  1:2725227/1‑140 (MQ=255)

                             cGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGaa                                                                                                           >  1:2511606/1‑140 (MQ=255)

                                  ttATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGa                                                                                                      >  1:4972899/1‑140 (MQ=255)

                                   tataAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGaa                                                                                                     <  1:2349666/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  2:309587/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  2:4651597/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  1:4318023/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  2:4036037/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  1:4500509/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGCCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  2:4188075/140‑1 (MQ=255)

                                          aTAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCg                                                                                                       >  1:2104437/1‑131 (MQ=255)

                                          aTAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTcc                                                                                              >  1:128704/1‑140 (MQ=255)

                                          aTAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTcc                                                                                              >  2:3291509/1‑140 (MQ=255)

                                               cAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTaaa                                                                                         <  1:3353110/140‑1 (MQ=255)

                                                  gATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAagca                                                                                      >  1:4679299/1‑140 (MQ=255)

                                                   aTAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAagcag                                                                                     >  2:891413/1‑140 (MQ=255)

                                                    tAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAagcagc                                                                                    <  1:2346921/140‑1 (MQ=255)

                                                      aaaaTGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCaa                                                                                  <  1:4769392/140‑1 (MQ=255)

                                                           gTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATAcc                                                                             <  2:5251134/140‑1 (MQ=255)

                                                           gTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATAcc                                                                             <  2:773474/140‑1 (MQ=255)

                                                            ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  2:2059902/1‑140 (MQ=255)

                                                            ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  2:4012606/1‑140 (MQ=255)

                                                            ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  1:4548017/1‑140 (MQ=255)

                                                             tGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCgg                                                                           <  2:1292658/140‑1 (MQ=255)

                                                                     tGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATg                                                                   >  1:914225/1‑140 (MQ=255)

                                                                       cTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAACCGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgca                                                                 <  1:1477033/140‑1 (MQ=255)

                                                                         aaCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagc                                                               >  2:3493402/1‑140 (MQ=255)

                                                                          aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              <  2:1834262/140‑1 (MQ=255)

                                                                          aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              <  2:5322341/140‑1 (MQ=255)

                                                                          aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              <  1:515650/140‑1 (MQ=255)

                                                                          aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              <  1:427485/140‑1 (MQ=255)

                                                                              aGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTa                                                          >  2:5324576/1‑140 (MQ=255)

                                                                              aGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTa                                                          >  2:3368617/1‑140 (MQ=255)

                                                                              aGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTa                                                          >  2:3723929/1‑140 (MQ=255)

                                                                              aGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTa                                                          >  1:831254/1‑140 (MQ=255)

                                                                               gCACAGCTAGTGCGCGTCTGTCATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑AAA‑ACAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTaa                                                         >  2:4888998/1‑140 (MQ=255)

                                                                               gCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTaa                                                         >  2:1673710/1‑140 (MQ=255)

                                                                               gCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTaa                                                         >  1:3544876/1‑140 (MQ=255)

                                                                                 acaGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATGGTGAAATCGAAACACCCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGtcact                                                       >  2:1687754/1‑136 (MQ=255)

                                                                                 acaGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATGACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAatt                                                       >  1:3767662/1‑140 (MQ=255)

                                                                                 acaGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAatt                                                       >  1:31864/1‑140 (MQ=255)

                                                                                 acaGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAatt                                                       >  2:3723569/1‑140 (MQ=255)

                                                                                    gCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACC‑AAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAattatt                                                    >  2:2302435/1‑140 (MQ=255)

                                                                                    gCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAattatt                                                    >  2:4471959/1‑140 (MQ=255)

                                                                                    gCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑AAAGA‑AAGGAACTTCCTGACCCATGGTGAAATCGAATCACTCCTTAAAGCAGCAAAGACCGGGCCTCATCCGCCACGTAattatt                                                    >  2:3823144/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  2:933186/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  2:1571339/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:1402994/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:4714878/1‑140 (MQ=255)

                                                                                       aGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTc                                                 <  2:4363439/140‑1 (MQ=255)

                                                                                        gTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCt                                                >  1:4401907/1‑140 (MQ=255)

                                                                                        gTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCt                                                >  2:1000896/1‑140 (MQ=255)

                                                                                         tGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTg                                               >  1:1001131/1‑140 (MQ=255)

                                                                                         tGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTg                                               >  1:1002525/1‑140 (MQ=255)

                                                                                         tGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTCAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTg                                               >  2:4624143/1‑140 (MQ=255)

                                                                                            gcgTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGAct                                            <  1:4042733/140‑1 (MQ=255)

                                                                                             cgTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActt                                           >  2:1601210/1‑140 (MQ=255)

                                                                                             cgTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActt                                           >  1:1229187/1‑140 (MQ=255)

                                                                                             cgTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActt                                           >  2:5213283/1‑140 (MQ=255)

                                                                                               tCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCATCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttg                                         >  1:3159535/1‑140 (MQ=255)

                                                                                               tCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttg                                         >  2:691200/1‑140 (MQ=255)

                                                                                               tCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATAACGGGCCTCATGCAGCACGTAATTATTGTCTGActttg                                         >  1:3358308/1‑140 (MQ=255)

                                                                                               tCTGTAAGGATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCATGACCCATAGTTAAATCGAATAACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActgtg                                         >  2:3345419/1‑140 (MQ=255)

                                                                                                 tGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgct                                       <  2:2123548/140‑1 (MQ=255)

                                                                                                 tGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgct                                       <  1:1167044/140‑1 (MQ=255)

                                                                                                 tGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgct                                       <  2:2736243/140‑1 (MQ=255)

                                                                                                   tAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgcttt                                     >  2:2604270/1‑140 (MQ=255)

                                                                                                      ttATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGtt                                  <  2:136675/140‑1 (MQ=255)

                                                                                                        ataAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGtttt                                >  2:1109702/1‑140 (MQ=255)

                                                                                                        ataAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGtttt                                >  1:1652098/1‑140 (MQ=255)

                                                                                                         gaaGGGAAACACGATCCAGACTACTCCTGTTAA‑CTAAA‑A‑CAGCACCTTCCTCCCCCATCGTCAACTCCAATCACTCCTTAAAGCACTAAATACCGCGCCTCATGCAGCACGTAATTATTGTCTCACTTTGCTTTGTTTTa                               <  2:3876139/139‑1 (MQ=255)

                                                                                                             ggAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCa                           >  1:5157444/1‑140 (MQ=255)

                                                                                                             ggAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCa                           <  1:1798655/140‑1 (MQ=255)

                                                                                                             ggAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCa                           <  1:3526197/140‑1 (MQ=255)

                                                                                                             ggAAAAACGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCa                           >  1:4808336/1‑140 (MQ=255)

                                                                                                               aaaaaCGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATg                         <  2:2252731/140‑1 (MQ=255)

                                                                                                               aaaaaCGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATg                         <  2:2543868/140‑1 (MQ=255)

                                                                                                                aaaaCGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATgg                        >  1:3009488/1‑140 (MQ=255)

                                                                                                                 aaaCGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAGCTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGt                       >  2:4614530/1‑140 (MQ=255)

                                                                                                                 aaaCGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGt                       >  1:3287059/1‑140 (MQ=255)

                                                                                                                 aaaCGATGAAGAATAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGt                       >  1:3287096/1‑140 (MQ=255)

                                                                                                                        gaagaaTAAGGCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCggg                <  2:4822996/140‑1 (MQ=255)

                                                                                                                                 ggCTGATAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGaaa       >  2:720097/1‑140 (MQ=255)

                                                                                                                                      aTAA‑CAAAA‑A‑AAGGAACTTCCTGACGCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:4784977/1‑140 (MQ=255)

                                                                                                                                      aTAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGtt                                  <  2:2938655/108‑1 (MQ=255)

                                                                                                                                      aTAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:597064/1‑140 (MQ=255)

                                                                                                                                      aTAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  1:3406436/1‑140 (MQ=255)

                                                                                                                                      aTAA‑CAAAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  1:4479215/1‑140 (MQ=255)

CCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑AAA‑A‑AAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGT  >  NZ_CP025268/4542754‑4543026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`