BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	514,858	T→C	intergenic (‑35/‑111)	qmcA ← / → fetA	SPFH/Band 7/PHB domain protein/iron ABC transporter ATP‑binding protein FetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	514,858	0	T	C	100.0%	840.7 / NA	249	intergenic (‑35/‑111)	qmcA/fetA	SPFH/Band 7/PHB domain protein/iron ABC transporter ATP‑binding protein FetA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (121/128); total (121/128)

Only 100 of 249 total aligned reads displayed.

AAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGCTTC  >  NZ_CP025268/514722‑514993

  aGCGTTCTACTGTCCACTGATAGTCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAAt                                                                                                                                    <  1:5248654/140‑1 (MQ=255)

  aGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAAt                                                                                                                                    >  2:703556/1‑140 (MQ=255)

         taacgTCAACTGATCGGCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAGCCTCCTGTTGTACCGTCCATAGTCAGCAAAATTgctgct                                                                                                                             <  2:3036248/136‑1 (MQ=255)

            tGTCCATTGATACCCCAGCGGTACGATTTTGACACCCGCGCCTACAATAACCAGCGCATCAACAATTAGAATCGGGATAAAGAGATGTATCGAAAAAACCTTCTGTTGTATCGTGCATAAACAGCAAAATTGCTGGTTGa                                                                                                                          <  2:72179/140‑1 (MQ=255)

              tCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGAtt                                                                                                                        >  1:1490958/1‑140 (MQ=255)

              tCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGAtt                                                                                                                        >  2:665358/1‑140 (MQ=255)

              tCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGAtt                                                                                                                        >  2:1329491/1‑140 (MQ=255)

                cACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTaa                                                                                                                      >  1:5010948/1‑140 (MQ=255)

                cACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTaa                                                                                                                      >  1:2397465/1‑140 (MQ=255)

                 aCTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAACATTGCTGCTTGATTaaa                                                                                                                     <  1:2068317/140‑1 (MQ=255)

                 aCTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTaaa                                                                                                                     <  1:3637552/140‑1 (MQ=255)

                 aCTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTaaa                                                                                                                     <  1:1508857/140‑1 (MQ=255)

                 aCTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTaaa                                                                                                                     <  1:1585373/140‑1 (MQ=255)

                 aCTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTaaa                                                                                                                     <  2:2813195/140‑1 (MQ=255)

                 aCTAATAGCCCTGCGGCACGATTTTGACACCCTCGGCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAATATCAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTaaa                                                                                                                     <  2:4023885/140‑1 (MQ=255)

                   tGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACa                                                                                                                   >  1:4295826/1‑140 (MQ=255)

                   tGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACa                                                                                                                   <  2:4666448/140‑1 (MQ=255)

                   tGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACa                                                                                                                   >  1:1214638/1‑140 (MQ=255)

                     aTAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACaaa                                                                                                                 >  2:2615011/1‑140 (MQ=255)

                     aTAGCCCTGCGGAACAATATTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGGGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACaaa                                                                                                                 >  2:905984/1‑140 (MQ=255)

                      tAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAAt                                                                                                                <  2:3410062/140‑1 (MQ=255)

                        gCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATta                                                                                                              >  2:3511195/1‑140 (MQ=255)

                        gCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATta                                                                                                              >  1:275273/1‑140 (MQ=255)

                        gCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATta                                                                                                              >  2:463206/1‑140 (MQ=255)

                           cTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATAc                                                                                                           <  2:357719/140‑1 (MQ=255)

                             gCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGCTAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCt                                                                                                         >  1:2140866/1‑140 (MQ=255)

                             gCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAAt                                                                                                                                    <  1:1988558/113‑1 (MQ=255)

                             gCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCt                                                                                                         >  1:915057/1‑140 (MQ=255)

                             gCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCt                                                                                                         >  1:2334383/1‑140 (MQ=255)

                             gCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCGGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCt                                                                                                         >  2:1598300/1‑140 (MQ=255)

                                gTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGAt                                                                                                      <  1:642786/140‑1 (MQ=255)

                                gTACGATTTTGACAACCGCGCCGACAATGCCCAGCGCGACAAAAATGAGAACCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCCTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGAt                                                                                                      <  2:2856476/140‑1 (MQ=255)

                                  aCGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAACGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTa                                                                                                    <  1:4460967/140‑1 (MQ=255)

                                  aCGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTa                                                                                                    <  2:1105095/140‑1 (MQ=255)

                                  aCGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTa                                                                                                    <  1:4612436/140‑1 (MQ=255)

                                   tgATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGTATCGGGAGCAAGATCCGCATCGGAAAAACCTCCTGTTAGACTGTCAATAATCAGCAAAATTGTTGCTTGATTGAACAAATTATAATTGATAAc                                                                                                   >  2:5332192/2‑140 (MQ=255)

                                   cGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTAc                                                                                                   >  2:2258181/1‑140 (MQ=255)

                                   cGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTAc                                                                                                   >  2:2503172/1‑140 (MQ=255)

                                   cGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTAc                                                                                                   >  1:3683140/1‑140 (MQ=255)

                                   cGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGGACCGTCCATAATCAGCAAAATTGCTGCTGGATTAAACAAAGCATACCTGAGTAc                                                                                                   >  2:2258209/1‑140 (MQ=255)

                                      ttttGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGa                                                                                                >  1:4277376/1‑140 (MQ=255)

                                         tGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAag                                                                                             <  2:4375877/140‑1 (MQ=255)

                                             acCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAagagag                                                                                         <  1:4194958/140‑1 (MQ=255)

                                              cccGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGt                                                                                        <  2:2008081/140‑1 (MQ=255)

                                              cccGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGt                                                                                        <  1:4400272/140‑1 (MQ=255)

                                               ccGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGtt                                                                                       <  1:2999459/140‑1 (MQ=255)

                                               ccGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGtt                                                                                       <  2:2561993/140‑1 (MQ=255)

                                                cgcgCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTc                                                                                      <  2:317822/140‑1 (MQ=255)

                                                cgcgCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTc                                                                                      <  1:743653/140‑1 (MQ=255)

                                                cgcgCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTc                                                                                      <  1:2893204/140‑1 (MQ=255)

                                                cgcgCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTc                                                                                      >  1:1442152/1‑140 (MQ=255)

                                                    ccGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTccccc                                                                                  <  1:2473187/140‑1 (MQ=255)

                                                     cGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCt                                                                                 <  2:1559445/140‑1 (MQ=255)

                                                          aTGACCAGCGCGACGAAAATGAGAATCGGGATAAAGATAAGCATCGGAAACACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATCCCTGATTACTGAAAGGGAGTTCCCCCTTAGTc                                                                            <  2:1185099/140‑1 (MQ=255)

                                                             aCCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTg                                                                         >  2:3641822/1‑140 (MQ=255)

                                                             aCCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTg                                                                         >  2:471269/1‑140 (MQ=255)

                                                              ccAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGc                                                                        <  1:2497073/140‑1 (MQ=255)

                                                               cAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCg                                                                       <  1:4251288/140‑1 (MQ=255)

                                                               cAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCg                                                                       <  2:3317609/140‑1 (MQ=255)

                                                                aGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAACCCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                      <  2:3899094/140‑1 (MQ=255)

                                                                aGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                      <  2:3302010/140‑1 (MQ=255)

                                                                aGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGa                                                                      <  1:4647531/140‑1 (MQ=255)

                                                                 gcgcGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGaa                                                                     >  2:4511580/1‑140 (MQ=255)

                                                                  cgcgACAAAAATGAGAATGGGGATAAAGATAAGCATCGGCAAAACCTCCAGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGCTTACTGAAAGAGAGTTCCCCCTTCTTCCTGCGAAg                                                                    >  2:2729524/1‑140 (MQ=255)

                                                                  cgcgACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAg                                                                    >  1:378794/1‑140 (MQ=255)

                                                                       cAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGaaa                                                                                              <  2:3745485/109‑1 (MQ=255)

                                                                            aTGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGt                                                          >  1:2606468/1‑140 (MQ=255)

                                                                               agaATCGGGATAAACATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTACACACAGTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGtttt                                                       <  2:805762/140‑1 (MQ=255)

                                                                               agaATCGGAATAAAGATAAGCATCGGCAAAACCTCCTGCTGTACCGTCCATAATCAGCAAAATTGCTGCTTGCTTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCATGCGAAGCATAAACTGtttt                                                       >  2:3773368/1‑140 (MQ=255)

                                                                                 aaTCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTa                                                     >  1:4103771/1‑140 (MQ=255)

                                                                                    cGGGATAAAGATAAGCATCGGAAAAACATCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTa                                                  >  2:128006/1‑140 (MQ=255)

                                                                                         tAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAAt                                             <  1:190584/140‑1 (MQ=255)

                                                                                            aGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAg                                          <  1:538206/140‑1 (MQ=255)

                                                                                                 aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATGCCTGATTACTGAAAGAGAGTAACCCCTTATGCCTGCGAAGGATAAACGGTTTTTAGTAAAAATCAGaaaaa                                     <  2:4928629/140‑1 (MQ=255)

                                                                                                 aGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGATAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGaaaaa                                     >  1:4775882/1‑140 (MQ=255)

                                                                                                      cGGAAAAACCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGaa                                >  2:4575014/1‑140 (MQ=255)

                                                                                                            aaCCTCCTGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGa                          <  1:1747335/140‑1 (MQ=255)

                                                                                                              cctcctGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATg                        <  2:3059187/140‑1 (MQ=255)

                                                                                                              cctcccGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTCATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATg                        <  1:106550/140‑1 (MQ=255)

                                                                                                                 cctGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAg                     <  2:1468646/140‑1 (MQ=255)

                                                                                                                 cctGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAg                     <  1:1036487/140‑1 (MQ=255)

                                                                                                                 cctCTTGTACCGTCCATAATCCGCAAAATTGCTGCTTGATTAAACAAATTATTCCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAg                     <  1:803501/140‑1 (MQ=255)

                                                                                                                  ctGTTGTACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAgg                    >  1:3415581/1‑140 (MQ=255)

                                                                                                                      tgtACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGaaaa                <  1:3252058/140‑1 (MQ=255)

                                                                                                                        tACCGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATa              >  1:1432876/1‑140 (MQ=255)

                                                                                                                          ccGTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGTATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGt            >  2:399232/1‑140 (MQ=255)

                                                                                                                            gTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGTGAACAGCGATGCAGGAAAATAGTcc          >  1:738055/1‑140 (MQ=255)

                                                                                                                            gTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTct          >  1:4727495/1‑139 (MQ=255)

                                                                                                                            gTCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTcc          >  2:1201908/1‑140 (MQ=255)

                                                                                                                            gTCCATAATAAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTcc          <  2:1435503/140‑1 (MQ=255)

                                                                                                                             tCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCt         >  1:4055406/1‑140 (MQ=255)

                                                                                                                             tCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCt         >  1:2840873/1‑140 (MQ=255)

                                                                                                                             tCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCt         >  2:4416902/1‑140 (MQ=255)

                                                                                                                             tCCATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCt         >  2:1782307/1‑140 (MQ=255)

                                                                                                                             tCCATAAGCAGCAAGATTCCTGATGGGTTACACAAACTACACCAGATTACTGAAAGAGAGTGAACCCTTATTCCTGCGAAGGATAAAATGTTTTTAGTAAAAAGCAGAGAAAGGAAACAGCGATGGAGGAAAAGAGttcg         >  2:5085014/1‑137 (MQ=255)

                                                                                                                               cATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGaaaa                >  2:4948389/1‑131 (MQ=255)

                                                                                                                               cATAATCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGaaaa                <  1:4948389/131‑1 (MQ=255)

                                                                                                                                   aTCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGCtt   <  1:710878/140‑1 (MQ=255)

                                                                                                                                   aTCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGCtt   <  1:321654/140‑1 (MQ=255)

                                                                                                                                    tCAGCAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGCTTc  <  1:1602895/140‑1 (MQ=255)

AAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGACACCCGCGCCGACAATGACCAGCGCGACAAAAATGAGAATCGGGATAAAGATAAGCATCGGAAAAACCTCCTGTTGTACCGTCCATAATCAGTAAAATTGCTGCTTGATTAAACAAATTATACCTGATTACTGAAAGAGAGTTCCCCCTTATTCCTGCGAAGGATAAACTGTTTTTAGTAAAAATCAGAAAAAGGGAACAGCGATGCAGGAAAATAGTCCTTTGCTTC  >  NZ_CP025268/514722‑514993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`