BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	521,513	C→T	A683V (GCG→GTG)	ybbP →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	521,513	0	C	T	97.8%	846.8 / NA	232	A683V (GCG→GTG)	ybbP	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (82/144); minor base G (1/4); total (84/148)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

AGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTT  >  NZ_CP025268/521377‑521648

aGTTTCCGCTGCGAGACTGGCATCGGCATGTTGACACAACTCCACCGCCCGTTCCCGACCATTAGCCTGTTAGATCTTGGCGCGCTTTTAAACCAGGACGGTCAGGTGCTGGAGCAGGTAAGACGGGCGCTGGAAGTGAt                                                                                                                                      <  2:1404753/140‑1 (MQ=255)

  tttGCGCTGGGAGAATGGCAACGGCATGTTGACACACCTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGAtgg                                                                                                                                    <  1:2272004/140‑1 (MQ=255)

    tCCGCTGGCAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCTTTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCATGTAAGTCGGGCGCTGGAAGTGAtggtg                                                                                                                                  <  2:3921843/140‑1 (MQ=255)

       gCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTa                                                                                                                               <  1:1057814/140‑1 (MQ=255)

        cTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTAc                                                                                                                              <  2:2744142/140‑1 (MQ=255)

           ggAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTgg                                                                                                                           >  2:749062/1‑140 (MQ=255)

           ggAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTgg                                                                                                                           >  2:388689/1‑140 (MQ=255)

           ggAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTgg                                                                                                                           >  1:1971821/1‑140 (MQ=255)

            gagaATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTAc                                                                                                                              <  2:899007/136‑1 (MQ=255)

             agaATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTc                                                                                                                         <  1:3270620/140‑1 (MQ=255)

             agaATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTc                                                                                                                         <  1:3588907/140‑1 (MQ=255)

             agaATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTc                                                                                                                         <  1:3932191/140‑1 (MQ=255)

                  ggCAACGGCATGTTGCCACAACTCACCCGCCAGTTCCCGACCATTAGCCTGTTAGCTATTGGCGCGATTTTAACACAGGTCGGTCAGGTGCTGGAGCAGGTCAGTCGGGCGCTGGAAGTGATGGTGGTCCTGGTCACCGc                                                                                                                    <  1:1385891/140‑1 (MQ=255)

                  ggCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGc                                                                                                                    <  2:3345520/140‑1 (MQ=255)

                     aaCGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTg                                                                                                                 <  2:3664337/140‑1 (MQ=255)

                      aCGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGc                                                                                                                <  1:1891894/140‑1 (MQ=255)

                       cGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCg                                                                                                               >  2:2068222/1‑140 (MQ=255)

                         gCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGt                                                                                                             >  2:5147884/1‑140 (MQ=255)

                          cATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTa                                                                                                            <  1:1493510/140‑1 (MQ=255)

                                 acacAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgct                                                                                                     <  1:2330138/140‑1 (MQ=255)

                                      aCTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgctgttgc                                                                                                >  2:3155084/1‑140 (MQ=255)

                                       cTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATgttgctgttgct                                                                                               <  1:1705777/140‑1 (MQ=255)

                                       cTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATgttgctgttgct                                                                                               >  2:2844433/1‑140 (MQ=255)

                                       cTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATgttgctgttgct                                                                                               >  1:2894711/1‑140 (MQ=255)

                                           aCCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGca                                                                                           <  1:688622/140‑1 (MQ=255)

                                           aCCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGca                                                                                           <  1:1344236/140‑1 (MQ=255)

                                            ccGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGcac                                                                                          <  2:1205682/140‑1 (MQ=255)

                                            ccGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGcac                                                                                          >  1:1689236/1‑140 (MQ=255)

                                            ccGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGcac                                                                                          <  1:2242898/140‑1 (MQ=255)

                                              gCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcag                                                                                        <  2:4798151/140‑1 (MQ=255)

                                                cAGTTCCCGACCCTTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggt                                                                                      <  1:1007344/140‑1 (MQ=255)

                                                 aGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggtg                                                                                     <  2:1607304/140‑1 (MQ=255)

                                                    tCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggtgcag                                                                                  <  2:2522588/140‑1 (MQ=255)

                                                      ccGACCATTAGGCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggtgcaggt                                                                                <  2:2604187/140‑1 (MQ=255)

                                                      ccGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggtgcaggt                                                                                <  1:4645702/140‑1 (MQ=255)

                                                         aCCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTggg                                                                             >  2:3549695/1‑140 (MQ=255)

                                                         aCCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTggg                                                                             >  1:1736667/1‑140 (MQ=255)

                                                             ttAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATg                                                                         >  1:1258816/1‑140 (MQ=255)

                                                             ttAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATg                                                                         >  2:3420011/1‑140 (MQ=255)

                                                              tAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgc                                                                        >  1:3143467/1‑140 (MQ=255)

                                                                  cTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtca                                                                    <  1:4151047/140‑1 (MQ=255)

                                                                     ttAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagcg                                                                 >  2:3250487/1‑140 (MQ=255)

                                                                     ttAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagcg                                                                 >  1:3826407/1‑140 (MQ=255)

                                                                     ttAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagcg                                                                 >  2:3522183/1‑140 (MQ=255)

                                                                     ttAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCGGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagcg                                                                 >  1:855679/1‑140 (MQ=255)

                                                                       aGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagcgtc                                                               <  2:3875169/140‑1 (MQ=255)

                                                                        gATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagcgtca                                                              >  1:5285498/1‑140 (MQ=255)

                                                                          tatTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGtcatc                                                            <  2:2237856/140‑1 (MQ=255)

                                                                          tatTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGtcatc                                                            >  2:1115914/1‑140 (MQ=255)

                                                                           atTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGtcatca                                                           <  2:1069822/140‑1 (MQ=255)

                                                                           atTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGtcatc                                                            >  1:4825527/1‑139 (MQ=255)

                                                                           atTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGtcatc                                                            <  2:4825527/139‑1 (MQ=255)

                                                                           atTGGCGCGATTTTAAAACAGGGCGGTCAGGTGCTGGAGCATGTAAGTCGGGGGCTGGAAAGGATGGTGGTACTGGTCGCCGCCTGCGGTATGTAGCTGTGACGGTGACGGGGGCGGGTGGGAAGGCGTGAGCGtgatcg                                                           >  2:92706/1‑139 (MQ=255)

                                                                             tGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAgg                                                         <  1:3309259/140‑1 (MQ=255)

                                                                              ggCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGGAGGTGGGAATGCGTCAGCGTCATCAGGa                                                        <  2:3961247/140‑1 (MQ=255)

                                                                               gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAg                                                       >  1:1170397/1‑140 (MQ=255)

                                                                               gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAg                                                       >  1:3958772/1‑140 (MQ=255)

                                                                               gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAg                                                       >  1:5039926/1‑140 (MQ=255)

                                                                               gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGGGGGAATGCGTCAGCGTCATCAGGAg                                                       >  2:4362224/1‑140 (MQ=255)

                                                                               gcgcGATTTTAAAACAGGGCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGGCACCGCATGCGGTATGTTGTTGTTGCTGGCACAGGTGCTGGTGGGAATGCGTGAGCGTCATCAGGAg                                                       >  2:598061/1‑140 (MQ=255)

                                                                                cgcgATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGc                                                      <  2:2018246/140‑1 (MQ=255)

                                                                                  cgATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGCTGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCtg                                                    <  1:3800415/140‑1 (MQ=255)

                                                                                    aTTTTAAAACAGGTCGGTCAGGGGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCtggt                                                  >  2:4015775/1‑140 (MQ=255)

                                                                                       ttAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCTGGCGCTGGAAGGGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTggtggt                                               <  2:3109817/140‑1 (MQ=255)

                                                                                       ttAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTggtggt                                               <  1:3159907/140‑1 (MQ=255)

                                                                                               ggtcggtcCGGGGCTGGAGCAGGTAAGTCGGGCGGTGGAAGTGAGGGTGGTACTGGTCACCGCCTGCGGTATGTGGCTGCTGCTGGAACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGGGTGGCGca                                       <  2:1038294/140‑1 (MQ=255)

                                                                                               ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGca                                       >  1:5218763/1‑140 (MQ=255)

                                                                                               ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGca                                       >  1:2308313/1‑140 (MQ=255)

                                                                                               ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGca                                       >  2:2130682/1‑140 (MQ=255)

                                                                                               ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGca                                       >  1:2421237/1‑140 (MQ=255)

                                                                                               ggtcggtcAGGTGCTGGAGCAGGTAAGTCGCGCGCTGGAAGTGATGGTGGTACTCATCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGAAGGTGGGAATGCGTCAGCGTCATCAGGAGCTCGTGGTGTGGCGca                                       >  2:4625833/1‑140 (MQ=255)

                                                                                                gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGcac                                      <  1:5095289/140‑1 (MQ=255)

                                                                                                   ggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACt                                   <  1:2936702/140‑1 (MQ=255)

                                                                                                   ggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGCTCACCGCCTGCGGTATGTTGCTGTTGCGGGCACAGGTGCAGGGGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACt                                   <  2:3232904/140‑1 (MQ=255)

                                                                                                     tcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCg                                 >  1:3640475/1‑140 (MQ=255)

                                                                                                         gTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGc                             <  1:3373983/140‑1 (MQ=255)

                                                                                                            cTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCggg                          <  1:4410529/140‑1 (MQ=255)

                                                                                                              ggAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGa                        <  1:71107/140‑1 (MQ=255)

                                                                                                              ggAGCAGGTAAGGCGGGCGCTGGAAGTGATGGTGGTACTGGTCAGCGCATGCGGTATGTTGCTGTTGCTGGCACAGGTGCTGGTGGCAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGa                        <  2:3187885/140‑1 (MQ=255)

                                                                                                               gAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGaa                       >  2:4458435/1‑140 (MQ=255)

                                                                                                                   aGGTAAGTCGGGCGCTGGAAGTTATGGTGGTACTAGTCACCGCCTGCGCTATTTTGCTTTTGCTGTCACAGATTCAGGTGTGAATACGTAAGCGTCATCAGGAGCTGGTGGTGTAGCGCACACTCGGTGCGGGGaaaaaa                   <  2:2580883/140‑1 (MQ=255)

                                                                                                                   aGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGaaaaaa                   <  2:5039926/140‑1 (MQ=255)

                                                                                                                   aGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGaaaaaa                   <  1:900730/140‑1 (MQ=255)

                                                                                                                    ggTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAAc                  >  1:1525058/1‑140 (MQ=255)

                                                                                                                    ggTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAAc                  >  2:1268958/1‑140 (MQ=255)

                                                                                                                    ggTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAAc                  >  1:2495915/1‑140 (MQ=255)

                                                                                                                    ggTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAAc                  >  1:343094/1‑140 (MQ=255)

                                                                                                                        aGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCGCCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACGCTCGGTGCGGGGAAAAAActgct              <  2:2813173/140‑1 (MQ=255)

                                                                                                                        aGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAActgct              <  1:2560551/140‑1 (MQ=255)

                                                                                                                          tCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgct                                                                                                     >  1:1129721/1‑51 (MQ=255)

                                                                                                                          tCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGAACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACCCTCGGTGCGGGGAAAAAACggctgc            <  1:4822723/140‑1 (MQ=255)

                                                                                                                           cGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTTTGTAGCCGTTGCTGGCACAGGCGCCGGTGGGAATGCGTCAGCGTCGTCAGGAGCTGGTGGTGTGGCCCACTCTTGGTGCGGGGAAAAAACTGCTGCg           <  1:3192167/140‑1 (MQ=255)

                                                                                                                             ggCGCCGGAAGGGATGGGGGTACGCGGCGGCGGTTGGGGTATGGTGCGGTTGCGGGCACGGGTGCAGGTGGGAAGGCGTCAGCGGCATCGGGAGCGGGTGGTGTGGCGCACACGCGGTGCGGGGAAAAAACTGCTGCGTa         <  2:1964855/140‑1 (MQ=255)

                                                                                                                               cgcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCTGTGCGGGGAAAAAACTGCTGCGTAcc       >  1:5141868/1‑140 (MQ=255)

                                                                                                                               cgcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTAcc       <  2:4150474/140‑1 (MQ=255)

                                                                                                                               cgcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTAcc       >  1:5212811/1‑140 (MQ=255)

                                                                                                                               cgcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTAcc       >  1:2797621/1‑140 (MQ=255)

                                                                                                                               cgcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTAcc       >  1:2393028/1‑140 (MQ=255)

                                                                                                                                gcTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCa      >  1:3130040/1‑140 (MQ=255)

                                                                                                                                    gAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGtt  >  2:437731/1‑140 (MQ=255)

AGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCACGTT  >  NZ_CP025268/521377‑521648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`