BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	550,382	A→G	298 (TAA→TAG)	ybcF →	carbamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	550,382	0	A	G	99.0%	1309.9 / NA	395	298 (TAA→TAG)	ybcF	carbamate kinase
Reads supporting (aligned to +/- strand): ref base A (0/2); major base G (156/232); minor base T (3/1); total (159/236)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 395 total aligned reads displayed.

CGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGT  >  NZ_CP025268/550247‑550517

cGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGt                                                                                                                                      <  1:968056/140‑1 (MQ=255)

cGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGt                                                                                                                                      <  1:3629689/140‑1 (MQ=255)

cGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGt                                                                                                                                      <  2:5188244/140‑1 (MQ=255)

cGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGt                                                                                                                                      <  1:1738885/140‑1 (MQ=255)

cGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGt                                                                                                                                      <  2:4141500/140‑1 (MQ=255)

 gATGGTTCGATGGGGCTGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAATCCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACACTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTa                                                                                                                                     <  1:2956239/140‑1 (MQ=255)

 gATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTa                                                                                                                                     >  1:789267/1‑140 (MQ=255)

 gATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTa                                                                                                                                     <  1:1354063/140‑1 (MQ=255)

   tGGTTCGGTGGGGCCGAATGTAACGGCGGTGAGTAGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGACTTGAAGAGACGGTGGCGGGCGAAGCGGGGACCTGTATTACGCTGTAGTCGTAgg                                                                                                                                   <  1:567237/140‑1 (MQ=255)

     gTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCa                                                                                                                                 <  2:1295396/140‑1 (MQ=255)

       tCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCAtt                                                                                                                               <  1:2702204/140‑1 (MQ=255)

        cGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTa                                                                                                                              >  1:3970746/1‑140 (MQ=255)

        cGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTa                                                                                                                              >  1:2347781/1‑140 (MQ=255)

              gggcGAGTGTAACGTCGCGTAGTGGTTATGTCAGAAGCCGTGGGAAAGCCGCGTGGGT‑TGGGGCGTTAGGGCGAATTGATAAGCCGCTCGCGGGCGGTGTTGGGACCGGTATTGCTCGGTAAGCGTAGGCAGTAGGCAtt                                                                                                                        <  2:1131053/137‑1 (MQ=255)

                ccGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGGGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCCAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTtg                                                                                                                      >  1:3427341/1‑140 (MQ=255)

                ccGAATGTAACGGCGGTGAGGGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATGTCGCTGTAGTCGTAGGCATTAGACATTtg                                                                                                                      >  1:2765130/1‑140 (MQ=255)

                   aaTGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGc                                                                                                                   >  2:1613566/1‑140 (MQ=255)

                    aTGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGATAAAGCCGTGTGGAT‑TGGGGCGTTATCGCGAATTGAAGGGACGCAGGCGGGCGAAGCGCGGAACTGTATTTCGCTGTATTCCTCGGCATTGGACATGTGTGcc                                                                                                                  <  2:2635921/140‑1 (MQ=255)

                         aCGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATg                                                                                                             >  2:230356/1‑140 (MQ=255)

                          cggcggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGc                                                                                                            <  2:2991246/140‑1 (MQ=255)

                          cggcggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGc                                                                                                            <  2:5259925/140‑1 (MQ=255)

                             cggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGAc                                                                                                         >  2:4379281/1‑140 (MQ=255)

                              ggTGAGTGGTTATGTCAGAAGCCGTGGTAACCCCGCGTGCAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACg                                                                                                        <  2:630930/140‑1 (MQ=255)

                               gTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGc                                                                                                       >  1:4419826/1‑140 (MQ=255)

                               gTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGc                                                                                                       >  1:1630218/1‑140 (MQ=255)

                               gTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTAGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGc                                                                                                       >  2:4934755/1‑140 (MQ=255)

                                  aGTGGTTATGTCGGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTg                                                                                                    >  2:2396797/1‑140 (MQ=255)

                                   gTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGa                                                                                                   <  1:419571/140‑1 (MQ=255)

                                   gTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGa                                                                                                   <  1:2159102/140‑1 (MQ=255)

                                   gTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGa                                                                                                   <  2:841955/140‑1 (MQ=255)

                                       ttATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGAcgcg                                                                                               >  1:3076244/1‑140 (MQ=255)

                                         aTGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTATTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑c                                                                                            >  2:4804589/1‑140 (MQ=255)

                                         aTGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑c                                                                                            >  1:3516409/1‑140 (MQ=255)

                                          tGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGGTATCGCGAATGGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCAGTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑Ct                                                                                           >  2:1480423/1‑140 (MQ=255)

                                            tCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCTTGATGCGACGCTTGACGCGT‑CTTa                                                                                         >  1:3875140/1‑140 (MQ=255)

                                            tCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTa                                                                                         >  1:6607/1‑140 (MQ=255)

                                             cAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGATGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTAt                                                                                        <  1:335679/140‑1 (MQ=255)

                                              ggaggCCGTGGTAAGCCCG‑GTGGGTGTGGGGCGTGATCGCGAGTTGGAGAGACGCTGGCGGGCGACGCGGGGACCGGGGTTTGGCGGTAGTGGTAGGCATTAGACGTGTGGGCCTGATGCTCGGCTTGACGCGG‑CTGATc                                                                                       <  2:2200488/136‑1 (MQ=255)

                                              aGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAGTTGTAGCGACGCTGGCGGGCGCAGCGGGGACCTGTATTTCGCTGTCGTCGTAGGCATTAGACATTTTTGCATGATGCGACGCTTGACGCGT‑CTTATc                                                                                       <  1:3199802/140‑1 (MQ=255)

                                              aGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATc                                                                                       >  2:4071430/1‑140 (MQ=255)

                                              aGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATc                                                                                       <  1:2996522/140‑1 (MQ=255)

                                              aGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATc                                                                                       <  2:5245026/140‑1 (MQ=255)

                                              aGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATc                                                                                       <  1:1922744/140‑1 (MQ=255)

                                              aGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATc                                                                                       <  2:4216783/140‑1 (MQ=255)

                                              aGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCGGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGt                                                                                              >  2:383785/1‑134 (MQ=255)

                                              aGAAGCAGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATAGCGAATTGAAGAGACGCTGGAGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGAATTAGACATTTGTGCATGATGAGACGCTTGACGCGT‑CTTATc                                                                                       <  2:3169195/140‑1 (MQ=255)

                                                aaGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAg                                                                                     >  1:4704037/1‑140 (MQ=255)

                                                aaGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAg                                                                                     <  2:1534482/140‑1 (MQ=255)

                                                aaGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAg                                                                                     >  2:497659/1‑140 (MQ=255)

                                                aaGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGGTAGCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCGGTATTTCGCTGTAGTCGTAGGCATTAGACATTGGGGCCTGATTCGACGCTTGACGCGG‑CTTATCAg                                                                                     >  2:3080226/1‑140 (MQ=255)

                                                     gTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTa                                                                                >  1:2065414/1‑140 (MQ=255)

                                                      tGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTAc                                                                               >  2:4512395/1‑140 (MQ=255)

                                                      tGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTAc                                                                               >  1:2835408/1‑140 (MQ=255)

                                                      tGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTAc                                                                               >  1:1187339/1‑140 (MQ=255)

                                                                 cgTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCg                                                                    >  1:5230123/1‑140 (MQ=255)

                                                                 cgTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCg                                                                    >  2:1725133/1‑140 (MQ=255)

                                                                  gTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGc                                                                   >  1:5166470/1‑140 (MQ=255)

                                                                    ggAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCAt                                                                 <  2:3875408/140‑1 (MQ=255)

                                                                    ggAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCAt                                                                 <  1:4639514/140‑1 (MQ=255)

                                                                          ggggCGTTGTCGCGACTTGAAGAGCCGCTGGTGGGCGAAGCGGGGACCTGTATTTCGCTGGAGTGGGAGGCATTAGACATTTGTGCTTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGc                                                            <  2:3059622/140‑1 (MQ=38)

                                                                           gggCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCa                                                           >  1:2510815/1‑140 (MQ=255)

                                                                            ggCGCTATCGCGAATTGAAGAGACGCTGGCGGGCGACGCGGGGACCTGTATTTCGCTCTAGTCGTAGGCATTAGACATCTGTGCCTGATGCGACGCTTGCCGCGT‑CTTATCAGGCCTACACCCGGTGCCGCATCCGGCaa                                                          <  2:1239655/140‑1 (MQ=38)

                                                                             gCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAAt                                                         <  2:3731049/140‑1 (MQ=255)

                                                                             gCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCCGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCAGATGCGACGCTTGACGCGC‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAAt                                                         <  2:4622514/140‑1 (MQ=255)

                                                                             gCGTTAGCGCGAAGTGGAGAGACGCGGGAGGGCGAAGCGGGGACGTGTATTTCGCTGTAGTCGTAGGCATGAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAAt                                                         <  2:138712/140‑1 (MQ=38)

                                                                              cGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACCTTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAAtt                                                        <  1:4249570/140‑1 (MQ=255)

                                                                               gTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTg                                                       <  1:3800747/140‑1 (MQ=255)

                                                                                ttATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTgg                                                      >  1:3321259/1‑140 (MQ=255)

                                                                                 tATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGt                                                     >  1:4668155/1‑140 (MQ=255)

                                                                                     gcgAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcac                                                 <  1:1428751/140‑1 (MQ=255)

                                                                                     gcgAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcac                                                 <  2:1946185/140‑1 (MQ=255)

                                                                                           tGAAGAGACGCTGGCGGGCGACGCTGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATCTGTGCCTGATGCGCCGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGcc                                           <  2:4003717/140‑1 (MQ=39)

                                                                                           tGAAGAGACGCTGGCGGGCGAAGCGGGGACCGGTATTGCGCTGTAGTCGTAGGCATGAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGcc                                           <  1:5311009/140‑1 (MQ=255)

                                                                                                          gggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                            <  2:3054118/140‑1 (MQ=255)

                                                                                                          gggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                            <  1:1029454/140‑1 (MQ=255)

                                                                                                          gggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                            <  2:3635543/140‑1 (MQ=255)

                                                                                                          gggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGGGCCTGATGCGACGCTTGACGCGT‑CGTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                            <  2:3533306/140‑1 (MQ=255)

                                                                                                            gcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACg                          >  2:3976375/1‑140 (MQ=255)

                                                                                                            gcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACg                          <  1:4078398/140‑1 (MQ=255)

                                                                                                            gcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTACCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACg                          <  1:2626375/140‑1 (MQ=255)

                                                                                                                    gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACTCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                  <  1:4795814/140‑1 (MQ=255)

                                                                                                                    gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                  <  2:3288037/140‑1 (MQ=255)

                                                                                                                    gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                  <  1:3238268/140‑1 (MQ=255)

                                                                                                                    gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                  <  1:903718/140‑1 (MQ=255)

                                                                                                                    gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                  <  2:2469556/140‑1 (MQ=255)

                                                                                                                    gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                  <  2:2695659/140‑1 (MQ=255)

                                                                                                                    agccacTGGATTTCGTCGTAGTCTTGGCCATTAGGACTTTGTGCCTGATGCCACGCTTG‑CCCGTGCTGATCAGTCCTACACCCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGTGATGCTTGACGCATCTTAt                  <  2:712363/135‑1 (MQ=255)

                                                                                                                     ggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATc                 <  2:18735/140‑1 (MQ=255)

                                                                                                                     ggACCTCTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATc                 <  1:2799059/140‑1 (MQ=255)

                                                                                                                       aCCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAg               <  1:4569261/140‑1 (MQ=255)

                                                                                                                         cTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGc             <  1:1101327/140‑1 (MQ=255)

                                                                                                                         cTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGc             <  2:2777/140‑1 (MQ=255)

                                                                                                                         cTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGc             <  2:2096820/140‑1 (MQ=255)

                                                                                                                           gTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCt           <  2:3427341/140‑1 (MQ=255)

                                                                                                                           gTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCt           >  1:743296/1‑140 (MQ=255)

                                                                                                                                tCGCTGTCGTCGTAGGCATTAGACATTTGTGCCTGATGCGACACTTGACGCGT‑CATATCAGGCCTAAAACCGGTGAAGCATCCGGCAATTGGTGCACAATGCCTGACGCAATGCTTGACGCATCTTATCAGGCCTACAAt      <  2:4692954/140‑1 (MQ=38)

                                                                                                                                tCGCTGTAGTCGTAGGCATTAGACCTTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACACTGCCTGATGCGATGCCTGACGCATCTTATCAGGCCTACAAt      <  2:1029203/140‑1 (MQ=255)

                                                                                                                                tCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt      <  2:5103715/140‑1 (MQ=255)

                                                                                                                                    tGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGt  >  1:5042272/1‑140 (MQ=255)

                                                                                                                                    tGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGt  <  1:1327108/140‑1 (MQ=255)

CGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT‑TGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGT‑CTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGT  >  NZ_CP025268/550247‑550517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`