BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	695,324	A→G	392 (TAA→TAG)	ubiF →	3‑demethoxyubiquinol 3‑hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	695,324	0	A	G	100.0%	799.5 / NA	241	392 (TAA→TAG)	ubiF	3‑demethoxyubiquinol 3‑hydroxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (135/106); total (135/106)

Only 100 of 241 total aligned reads displayed.

AGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGA  >  NZ_CP025268/695187‑695460

aGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGcc                                                                                                                                         >  2:3650493/1‑140 (MQ=255)

  cGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCtt                                                                                                                                       >  1:2438460/1‑140 (MQ=255)

     tATGGATCTGTTTTATGCTGGATGCTGCAATAATCTGCCAGCACTGCGTTTTATGGGTAAGCTCGTGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTCTAGCCTTaga                                                                                                                                    >  2:780834/1‑138 (MQ=255)

     tATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTaca                                                                                                                                    >  1:2246795/1‑140 (MQ=255)

            cTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGc                                                                                                                             <  2:198076/140‑1 (MQ=255)

             tGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGcc                                                                                                                            <  2:5022207/140‑1 (MQ=255)

               ttttATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCgg                                                                                                                          >  2:2603359/1‑140 (MQ=255)

               ttttATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCgg                                                                                                                          >  1:3176892/1‑140 (MQ=255)

               ttttATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCgg                                                                                                                          >  2:4910230/1‑140 (MQ=255)

               ttttATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCgg                                                                                                                          >  1:4850113/1‑140 (MQ=255)

               ttttATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCgg                                                                                                                          >  1:4924501/1‑140 (MQ=255)

                tttATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGa                                                                                                                         <  1:439995/140‑1 (MQ=255)

                 gtATGCCGGATTGAGCAATAATTTGCCACCACTGCGTTGTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGCGCTGGCGTGTTGAAACGACAGGTGCAGAAAAATGCGTTAGGGTTGTAGCCTTACAACATGGCCTGag                                                                                                                        >  2:1157338/2‑139 (MQ=255)

                      ccGGATTCAGCAATAAGCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTg                                                                                                                   <  1:2968346/140‑1 (MQ=255)

                       cGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGc                                                                                                                  >  1:2770119/1‑140 (MQ=255)

                         gATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGGGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCa                                                                                                                >  2:3348262/1‑140 (MQ=255)

                         gATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCa                                                                                                                >  1:2690842/1‑140 (MQ=255)

                         gATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCa                                                                                                                >  2:2308169/1‑140 (MQ=255)

                            tCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAAcc                                                                                                             <  2:166684/140‑1 (MQ=255)

                             cAGCAATTGTCTGCCAACACGGCGTTTTATGTGTAATCTCGGGTTGATGTCGGCGGCGAGTGCTGGCGTGTTGAAACGGCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCg                                                                                                            <  2:669417/140‑1 (MQ=255)

                              aGCAATAATCTGCCACCTCTGCGTTCGATGCGTAAGCTCGGGTTAATGGGGGCGGAGCGTGCTGGCGTGTTGAAGTGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGt                                                                                                           <  2:136931/140‑1 (MQ=255)

                                  ataatCTTCCACCACTGCGTTTTATGCCTAATCTCGAGTTAATGGCGGCGGAGCGTGCTGGCGTTTTGAAACGTCAGGCGCGGAAATATGCGTGAGGGTTGTAGCCTTACAACACTGCCGGATGCGTGCCAACCGT‑AGGt                                                                                                      <  2:4803168/140‑1 (MQ=255)

                                  ataatCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGt                                                                                                      <  1:262126/140‑1 (MQ=255)

                                     atCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCgg                                                                                                   >  1:1009627/1‑140 (MQ=255)

                                        tGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATa                                                                                                >  2:4177745/1‑140 (MQ=255)

                                          ccaccaCTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAg                                                                                              >  1:3610941/1‑140 (MQ=255)

                                          ccaccaCTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAg                                                                                              >  2:2997888/1‑140 (MQ=255)

                                          ccaccaCTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAg                                                                                              >  1:4362670/1‑140 (MQ=255)

                                          ccaccaCTGCGCTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGAAGTGTTGAACCATCATGCGCTGAAATATGCGTGAGTGTTGTAGCCTTACAACATTGCCGGATACGTGCCAACCGT‑AGGTCGGATagg                                                                                              >  2:5177677/1‑138 (MQ=255)

                                                cTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCgg                                                                                        >  1:5220656/1‑140 (MQ=255)

                                                  gCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCa                                                                                      >  2:265265/1‑140 (MQ=255)

                                                   cGTTTTATGCGTAATCTCGGGGTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAg                                                                                     >  1:2276089/1‑140 (MQ=255)

                                                     ttttATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCg                                                                                   >  2:3949285/1‑140 (MQ=255)

                                                          tGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCa                                                                              >  2:1768968/1‑140 (MQ=255)

                                                           gCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCAt                                                                             >  1:3325346/1‑140 (MQ=255)

                                                             gTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGTGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTTACAACCGT‑AGGCCGGATAAGACGCGGCAGCGTCGCATcc                                                                           >  2:229438/1‑140 (MQ=255)

                                                             gTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGTTCGGATAAGACGCTGCAGCGTCGCGTcc                                                                           >  1:4326851/1‑140 (MQ=255)

                                                               aaTCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGCAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGa                                                                         <  1:2203613/140‑1 (MQ=255)

                                                                   tcGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACAtt                                                                     <  2:3465/140‑1 (MQ=255)

                                                                   tcGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACAtt                                                                     <  2:3305035/140‑1 (MQ=255)

                                                                    tgggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                    <  1:4718560/139‑1 (MQ=255)

                                                                    cGGGTTCATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGGCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                    <  2:351040/140‑1 (MQ=255)

                                                                    cGGGTTAATGGGGGCGGAGCGTGCTGGCGTGTTGACACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCGACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                    <  2:5126034/140‑1 (MQ=255)

                                                                    cGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                    <  1:4538391/140‑1 (MQ=255)

                                                                    cGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                    <  2:2695734/140‑1 (MQ=255)

                                                                    cGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                    <  1:1761918/140‑1 (MQ=255)

                                                                    cGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                    <  1:3878931/140‑1 (MQ=255)

                                                                    cGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                    <  2:1867939/140‑1 (MQ=255)

                                                                    cGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                    <  1:2897683/140‑1 (MQ=255)

                                                                    cGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                    <  2:4377358/140‑1 (MQ=255)

                                                                    cGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                    <  2:1055002/140‑1 (MQ=255)

                                                                    agggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAAAGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCGAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                    <  1:2864591/139‑1 (MQ=255)

                                                                     gggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGa                                                                   >  1:1639054/1‑140 (MQ=255)

                                                                     gggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGa                                                                   >  1:5170614/1‑140 (MQ=255)

                                                                     gggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGAGAAGACGCGGCAGCGTCGCATCCGACATTGa                                                                   >  1:586317/1‑140 (MQ=255)

                                                                      ggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGaa                                                                  >  1:2899586/1‑140 (MQ=255)

                                                                       gTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAg                                                                 <  1:882820/140‑1 (MQ=255)

                                                                           aTGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATa                                                             >  1:1842834/1‑140 (MQ=255)

                                                                            tGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATaa                                                            <  2:4968903/140‑1 (MQ=255)

                                                                            tGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATaa                                                            >  1:3657126/1‑140 (MQ=255)

                                                                            tGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATaa                                                            <  1:3786280/140‑1 (MQ=255)

                                                                            tGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGCGCCAACCGT‑AGGTCGGAGAAGCCGCGGCAGCGTCGCATCCGACATTGAAGGATaa                                                            <  2:1159385/140‑1 (MQ=255)

                                                                            tGGCGGCAGATCGTGCTGGCGTGTTGAAACGTCCGGGGCTGGAATATGCGTTGGGGTTGGAGGCTGACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGAGGCGGCAGCGTCGCATCCGACATTGAAGGATaa                                                            <  2:2523869/140‑1 (MQ=37)

                                                                              gcgtcgGAGCGTGCTTGCGTGTTGAAACGTTAGGCGTTGAAATATGCGTTATGGTTGTAGCTTTTCAATGTTACCGGATCCACCCAAATCTTCACGTAAG‑GAGTCGGCGGCAGCGTCGCATCCGACATTGAAGGATAAGa                                                          <  2:1953310/140‑1 (MQ=255)

                                                                                 gcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACgt                                                       >  2:3185442/1‑140 (MQ=255)

                                                                                 gcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACgt                                                       >  2:1995054/1‑140 (MQ=255)

                                                                                 gcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACgg                                                       >  2:2478573/1‑139 (MQ=255)

                                                                                   ggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACgtgt                                                     <  2:2418288/140‑1 (MQ=255)

                                                                                        gTGCTGGCGTGTTGAAACGTCAGGCGGTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACa                                                <  2:1304440/140‑1 (MQ=255)

                                                                                        gTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTTCCGGATGCGTGCCAACCGT‑AGGTCGGATAATACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACa                                                <  2:4712792/140‑1 (MQ=255)

                                                                                        gTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGGCGTGTCAACa                                                <  2:2222642/140‑1 (MQ=255)

                                                                                         tGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACAt                                               >  1:2597660/1‑140 (MQ=255)

                                                                                          gCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGAGGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATc                                              <  2:5338248/140‑1 (MQ=255)

                                                                                               cGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAATATTGCCGGATGCGTGCAAACCGT‑AGGGCGGATAATACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCAtt                                         <  2:679348/140‑1 (MQ=255)

                                                                                               cGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCAtt                                         <  2:782186/140‑1 (MQ=255)

                                                                                               cGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCCGACGCGTGCCAACCGT‑ACGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGCAGGATAAGACGCGCCAACAGCGCagt                                         >  2:2872026/1‑138 (MQ=38)

                                                                                                 tgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCg                                       >  1:2089017/1‑140 (MQ=255)

                                                                                                   ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGAc                                     >  1:1187164/1‑140 (MQ=255)

                                                                                                   ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGAc                                     >  1:1990460/1‑140 (MQ=255)

                                                                                                   ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGAc                                     >  1:5257503/1‑140 (MQ=255)

                                                                                                   ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGAc                                     >  1:3366304/1‑140 (MQ=255)

                                                                                                   gtGAAACGTCAGGCGCGGAAATATGCGGTAGGGTGGTGGCATTACGACATTGCCGGATGCGTGGCAACCGT‑AGGCCGGATAAGACGCGGGAGCGTCGCATCCGACATTGCAGGTTCAGAAGGGACACCAGCTCATTCGag                                     >  2:4628033/2‑139 (MQ=255)

                                                                                                    tGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACa                                    <  1:3650493/140‑1 (MQ=255)

                                                                                                     gAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACAt                                   <  2:4250530/140‑1 (MQ=255)

                                                                                                          gTCAGGCGCTGAAATCTGCGTTAGGTTTGTAGCCTTAAAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtgaat                              <  2:5265137/140‑1 (MQ=255)

                                                                                                             gggCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCCACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtgaatgaa                           <  1:3030704/139‑1 (MQ=255)

                                                                                                             agtggcTGAACTATGAGTTAGGGTTGTAGCCTTCCAACATTGCCGGATGCGAGCCAACAGT‑AGGTCCAATAAGAACCGGCAGCGTCGCATCCGACATTGCAGGCTACGACCTGTCAACATCGCATTCGACATtgaatgaa                           <  2:4430029/136‑1 (MQ=255)

                                                                                                             aGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtgaatgaa                           >  1:3767775/1‑140 (MQ=255)

                                                                                                              ggCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAAc                          <  1:1096161/140‑1 (MQ=255)

                                                                                                                 gcTGAAATATGCGTTAGGGTTGTAGTCTTACAACGATGCCGCATGCGCCCCGACCGA‑CGCGCGGAGAAGCCGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCa                       <  2:4826241/140‑1 (MQ=255)

                                                                                                                 gcTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCAGACATTGAAGGATAAGTCGTGTCAACATCGCATTCGACATTGAATGAACGCa                       <  1:2883336/140‑1 (MQ=255)

                                                                                                                  cTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑Agg                                                                                                       >  1:703167/1‑59 (MQ=255)

                                                                                                                   tGAAATATGCGTTATGGTTGTGGCCTTACCACATTCGCGGACGCGTGCCACCAGT‑AAGTGGGATAAGCCGCGGCTGCGTAGCATCCGAAATTGTAGGATAAGACGTGTCAACATCGCATTCGACATTAAATGAACGCAGa                     <  2:5001653/140‑1 (MQ=255)

                                                                                                                          tGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCaa              >  1:3959928/1‑140 (MQ=255)

                                                                                                                          tGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCaa              >  1:4776401/1‑140 (MQ=255)

                                                                                                                          tGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGGGCCAACGGT‑CGGGCGGATAAGGCGGGGCAGCGTCGCATCGGTCATTGAAGGGTAAGACGGGGCAACAGCGCATTCGATATGGAATGAACGCAGAGAAggga              >  2:5141166/1‑137 (MQ=255)

                                                                                                                            cGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCGTCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCTACATTAAATAAACGCAGGAAAGGaaaa            >  2:4325889/1‑140 (MQ=39)

                                                                                                                               tAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGc         >  2:2215832/1‑140 (MQ=255)

                                                                                                                               tAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGc         >  2:2331943/1‑140 (MQ=255)

                                                                                                                                 gggTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTc       >  2:3007651/1‑140 (MQ=255)

AGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGT‑AGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGA  >  NZ_CP025268/695187‑695460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`