BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	71,946	A→G	255 (TAA→TAG)	yabI →	DedA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	71,946	0	A	G	99.8%	1365.6 / NA	406	255 (TAA→TAG)	yabI	DedA family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (147/257); minor base T (0/1); total (147/259)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 410 total aligned reads displayed.

ATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAA‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGT  >  NZ_CP025268/71810‑72081

attattTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAg‑gag                                                                                                                                      >  2:1338898/1‑140 (MQ=255)

 ttattTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑gaga                                                                                                                                     <  2:2859501/140‑1 (MQ=255)

 ttattTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑gaga                                                                                                                                     <  1:440732/140‑1 (MQ=255)

  aattTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCAGCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGAt                                                                                                                                    >  2:4692769/2‑140 (MQ=255)

    tttGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAg                                                                                                                                  <  2:1408117/140‑1 (MQ=255)

      tGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTc                                                                                                                                >  1:162175/1‑140 (MQ=255)

      tGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCAGCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTc                                                                                                                                >  1:4565108/1‑140 (MQ=255)

        tCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCtt                                                                                                                              >  2:3403476/1‑140 (MQ=255)

        tCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCtt                                                                                                                              >  2:2553048/1‑140 (MQ=255)

         cccGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTtg                                                                                                                             <  1:3771860/140‑1 (MQ=255)

            gcgGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGc                                                                                                                          >  1:1011758/1‑140 (MQ=255)

               gTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCggg                                                                                                                       <  1:345039/140‑1 (MQ=255)

                 cGTTTGTTGTGGGTGATGCCGTTGCTTTCTGCCCTCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGAGGCCGGTGTATATCGATATTTTGCGTAAAGTGGTGGGGGTTTAG‑GAGATAGTCTTGTGCGGGtt                                                                                                                     <  2:502324/140‑1 (MQ=255)

                  gTTTGTTGTGGCTGACGCCGTTGCTTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATCTTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGCTAGTCTTGTGCGGGTTg                                                                                                                    >  2:3748608/1‑140 (MQ=255)

                    ttgttgTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGcc                                                                                                                  <  2:309736/140‑1 (MQ=255)

                          tgGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGc                                                                                                            >  2:194634/1‑140 (MQ=255)

                          tgGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGc                                                                                                            >  1:3906338/1‑140 (MQ=255)

                            gCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGa                                                                                                          >  1:1317031/1‑140 (MQ=255)

                             cTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGAc                                                                                                         >  1:1697617/1‑140 (MQ=255)

                              tGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACg                                                                                                        >  2:169446/1‑140 (MQ=255)

                                 cGCCGTTGATTTCTGCCATCGGCGTGGTGTCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCtg                                                                                                     >  1:1126731/1‑139 (MQ=255)

                                 cGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTTGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGGAAAGTGGTTGGGGTTTAG‑GAGATAGTGTTGTGCGGGTTGCCTGATGCGACGCtt                                                                                                     >  1:2784684/1‑140 (MQ=255)

                                 cGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCtt                                                                                                     >  1:3956563/1‑140 (MQ=255)

                                   ccGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                                   <  1:1763594/140‑1 (MQ=255)

                                   ccGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                                   <  2:1243064/140‑1 (MQ=255)

                                   ccGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                                   <  1:387588/140‑1 (MQ=255)

                                      ttGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCcgc                                                                                                >  1:3510566/1‑140 (MQ=255)

                                             cTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGTTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                         <  2:2359952/140‑1 (MQ=255)

                                             cTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                         <  1:1214682/140‑1 (MQ=255)

                                                  aTCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGTCGCGTCTTATCAGGc                                                                                    <  2:1985310/140‑1 (MQ=255)

                                                  aTCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                    <  1:4967416/140‑1 (MQ=255)

                                                    cGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                  <  2:1161208/140‑1 (MQ=255)

                                                     ggCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                                 >  1:1499989/1‑140 (MQ=255)

                                                          ggtggCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACaaaa                                                                            <  2:2476816/140‑1 (MQ=255)

                                                              gcgcTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCa                                                                        <  1:1937140/140‑1 (MQ=255)

                                                               cgcTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCAc                                                                       <  1:566050/140‑1 (MQ=255)

                                                               cgcTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCAc                                                                       <  1:4801337/140‑1 (MQ=255)

                                                                 cTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTa                                                                     >  1:4242769/1‑140 (MQ=255)

                                                                 cTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTa                                                                     >  2:4950451/1‑140 (MQ=255)

                                                                     tggtgTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCg                                                                 <  2:3581356/140‑1 (MQ=255)

                                                                      ggtgTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGt                                                                >  1:1844630/1‑140 (MQ=255)

                                                                      ggtgTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGAAGCTTGCCGCGTCTTATCAGGCCTACACAACGCCCTAACCGt                                                                >  2:5157364/1‑140 (MQ=39)

                                                                          ttAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGt                                                                                              >  1:3320891/1‑106 (MQ=255)

                                                                          ttAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGt                                                            <  2:1368868/140‑1 (MQ=255)

                                                                              ttCGCCACCCGTTGATGCCGGTGTATATCGAGATTTTGCGTAAAGTGGCTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCCGGATCGACGCTTGCCGCGTCTTATCCGGCCCACAAAACGCACTCCCCGTAGGTCGGa                                                        >  2:2736118/1‑140 (MQ=255)

                                                                               tCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGAt                                                       <  1:5143866/140‑1 (MQ=255)

                                                                               tCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGAt                                                       <  1:1472630/140‑1 (MQ=255)

                                                                                   cACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGGGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAgg                                                   <  1:2568189/140‑1 (MQ=255)

                                                                                      ccGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGt                                                >  1:1967313/1‑140 (MQ=255)

                                                                                           gATGCGGGTGTATATCGATATTTTGAGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCAcg                                           <  2:628741/140‑1 (MQ=255)

                                                                                           gATGCCGGTGTATATCGTTATTTTGCGTAAAGGGGTTGGGGGTTAG‑GAGCTCGTCTTGTGCGGGTTCCCCGGTGCGACGCGTGCAGCGTCTTATCAGGGCTACAAAACGCACTACCCGTAGGTCGGATATGGCGTTCAcg                                           <  2:276353/140‑1 (MQ=255)

                                                                                           gATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCAcg                                           <  1:2733852/140‑1 (MQ=255)

                                                                                           gATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCAcg                                           <  2:3183148/140‑1 (MQ=255)

                                                                                           gATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCAcg                                           <  1:3155820/140‑1 (MQ=255)

                                                                                                cGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCa                                      >  2:5358027/1‑140 (MQ=255)

                                                                                                cGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCa                                      >  1:2253544/1‑140 (MQ=255)

                                                                                                     tataTCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                 <  2:460284/140‑1 (MQ=255)

                                                                                                     tataTCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                 <  1:4305529/140‑1 (MQ=255)

                                                                                                     tataTCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                 <  2:2278457/140‑1 (MQ=255)

                                                                                                     tataTCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                 <  1:814353/140‑1 (MQ=255)

                                                                                                     tataTCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGCGTTACCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                 <  1:3809792/140‑1 (MQ=255)

                                                                                                     tataTCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGCCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                 <  2:4367323/140‑1 (MQ=255)

                                                                                                     tataTCGATATTTTGAGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                 <  2:45454/140‑1 (MQ=255)

                                                                                                     tataTCGACATTTTGCGTAAAGTGGTTGGGGTTTAG‑GGGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTGCAGGCCGCATCCGa                                 <  2:3969580/140‑1 (MQ=37)

                                                                                                       tatCGATATTTTGCGTAAAGTCGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACa                               <  1:9854/140‑1 (MQ=255)

                                                                                                        atCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGCGTTGCCTGATGCGACGCCTGCCGCGTCTTATCAGGCCTTCAAAACGCACTACCCGTAGGTCGGTTACGGCGTTCACGCCGCATCCGACAg                              <  1:3200877/140‑1 (MQ=38)

                                                                                                         tCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGt                             <  2:2050483/140‑1 (MQ=255)

                                                                                                         tCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGt                             <  2:4130694/140‑1 (MQ=255)

                                                                                                         tCGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGt                             <  2:1508323/140‑1 (MQ=255)

                                                                                                          cGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTg                            <  2:2294913/140‑1 (MQ=255)

                                                                                                          cGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTg                            <  1:2935139/140‑1 (MQ=255)

                                                                                                          cGATATTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTg                            >  1:2185177/1‑140 (MQ=255)

                                                                                                            atatTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCa                          >  2:5266724/1‑140 (MQ=255)

                                                                                                            atatTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCa                          >  1:1559533/1‑140 (MQ=255)

                                                                                                            atatTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCa                          >  1:4495822/1‑140 (MQ=255)

                                                                                                            atatTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGCTCACGCCGCATCCGACAGTGCa                          >  2:2971380/1‑140 (MQ=255)

                                                                                                             tatTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCat                         >  2:4246877/1‑140 (MQ=255)

                                                                                                              atTTTGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCata                        <  2:5206971/140‑1 (MQ=255)

                                                                                                               ttttGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCGTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCatac                       >  1:4438657/1‑139 (MQ=255)

                                                                                                                 ttGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTa                     >  1:2061155/1‑140 (MQ=255)

                                                                                                                  tGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTaa                    >  2:917892/1‑140 (MQ=255)

                                                                                                                  tGCGTAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTaa                    >  2:1018956/1‑140 (MQ=255)

                                                                                                                      tAACGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCg                <  2:3274269/140‑1 (MQ=255)

                                                                                                                      tAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCg                <  1:4645194/140‑1 (MQ=255)

                                                                                                                      tAAAGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCg                <  1:3103496/140‑1 (MQ=255)

                                                                                                                       aaaGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGt               >  2:3509292/1‑140 (MQ=255)

                                                                                                                       aaaGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGt               >  1:2473864/1‑140 (MQ=255)

                                                                                                                        aaGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTa              <  2:2110992/140‑1 (MQ=255)

                                                                                                                        aaGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTa              <  1:4030357/140‑1 (MQ=255)

                                                                                                                        aaGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTa              <  2:5054970/140‑1 (MQ=255)

                                                                                                                        aaGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTa              <  1:21153/140‑1 (MQ=255)

                                                                                                                        aaGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTa              <  1:2839898/140‑1 (MQ=255)

                                                                                                                         aGTGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTaa             >  2:3645624/1‑140 (MQ=255)

                                                                                                                           tGGTTGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATc           <  2:224305/140‑1 (MQ=255)

                                                                                                                           gggTTGGGGTTTAC‑AAGATGGTCTTGTGCGGGTATCCTTATACGACGCTTGCCGAGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCAGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATc           <  2:1470746/139‑1 (MQ=25)

                                                                                                                              ttGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATcccc        >  1:138858/1‑140 (MQ=255)

                                                                                                                              ttGGGGTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATcccc        >  2:68048/1‑140 (MQ=255)

                                                                                                                                 gggTTTATGGAGTTAGTCTTGTGCTGTTTGCCTGATGCGACGCTTGCCGCTTCTTATCAGGCCTATAAAACGCATTACCCGTAGTTCGGATAAGGCGTTGACGCCGCATCCGACAGTGCATACTAACCCGTAAGCCCCaa      <  2:2634162/140‑1 (MQ=25)

                                                                                                                                  ggTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATa    >  2:3865323/1‑140 (MQ=255)

                                                                                                                                   gTTTAG‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAg   <  1:3645624/140‑1 (MQ=255)

ATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAA‑GAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGT  >  NZ_CP025268/71810‑72081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`