BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	801,562	C→A	L54I (CTT→ATT)	ybhJ →	hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	801,562	0	C	A	99.5%	706.5 / NA	185	L54I (CTT→ATT)	ybhJ	hydratase
Reads supporting (aligned to +/- strand): ref base C (0/1); major base A (66/117); minor base G (1/0); total (67/118)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

GCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGT  >  NZ_CP025268/801425‑801698

gCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTAACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAAtt                                                                                                                                        >  2:935325/1‑140 (MQ=255)

      ttCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATaaaattaaaatt                                                                                                                                  <  1:3894628/140‑1 (MQ=255)

      ttCTCGCCAGTAATAACGAAATAAGTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATaaaattaaaatt                                                                                                                                  <  1:3532587/140‑1 (MQ=255)

          cGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATGGCCTGGGCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGt                                                                                                                              >  1:2428118/1‑140 (MQ=255)

            ccAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGttt                                                                                                                            <  1:2092217/140‑1 (MQ=255)

             cAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTg                                                                                                                           <  1:1047782/140‑1 (MQ=255)

              aGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGCAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGa                                                                                                                          <  1:4913174/140‑1 (MQ=255)

              aGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGa                                                                                                                          <  2:352051/140‑1 (MQ=255)

               gTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAAGTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGGCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGaa                                                                                                                         >  2:1396929/1‑139 (MQ=255)

                taataaCGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGAtt                                                                                                                        >  1:1937045/1‑140 (MQ=255)

                    aaCGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCAtt                                                                                                                    <  1:2262535/140‑1 (MQ=255)

                       gAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGc                                                                                                                 <  2:2393206/140‑1 (MQ=255)

                          ataatTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTCTTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCctc                                                                                                              >  1:734195/1‑140 (MQ=255)

                          ataatTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATTAAATTAAAATTAAGTTTGATTCATTAGCctc                                                                                                              <  2:4071345/140‑1 (MQ=255)

                            aatTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCtctc                                                                                                            <  1:530420/140‑1 (MQ=255)

                            aatTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCtctc                                                                                                            <  1:77575/140‑1 (MQ=255)

                             atTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAATCCACAAAAGGCACTATTGCCTTGTTTATTCTCTCTTCGCATAATATGTCCGGAAATATGGATAAAACTAAGATCAAGTTTGATTCATTAGCCTCTCa                                                                                                           <  1:3195349/140‑1 (MQ=255)

                             atTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCa                                                                                                           <  1:847887/140‑1 (MQ=255)

                             atTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCa                                                                                                           <  1:4496142/140‑1 (MQ=255)

                             aatGCCGAAGATCATTTCACCGGCGAAATTAAAAAAGAATAAGCCAAAAACGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAAGACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCtctct                                                                                                           <  2:5058189/138‑2 (MQ=255)

                              ttGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCAc                                                                                                          >  1:1817684/1‑140 (MQ=255)

                                   gaagaaCATTTCGCCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATCCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGatat                                                                                                     <  2:4637830/140‑1 (MQ=255)

                                     agaaCATTTCACCGGCGAAATTAAAAAAGAAGGAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTa                                                                                                   >  2:1467851/1‑140 (MQ=255)

                                      gaaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTAc                                                                                                  <  1:1179263/140‑1 (MQ=255)

                                       aaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTAcc                                                                                                 <  1:5198982/140‑1 (MQ=255)

                                       aaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTAcc                                                                                                 <  2:3096962/140‑1 (MQ=255)

                                       aaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTAcc                                                                                                 <  2:759332/140‑1 (MQ=255)

                                       aaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTAcc                                                                                                 <  1:2049768/140‑1 (MQ=255)

                                         cATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCtt                                                                                               >  1:2033961/1‑140 (MQ=255)

                                           tttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttg                                                                                             >  2:2984945/1‑140 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                            <  1:1102916/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                            <  1:1610558/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                            <  1:1315988/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                            <  2:1351668/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                            <  2:1542611/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                            <  2:1952103/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                            <  1:2711055/140‑1 (MQ=255)

                                              cACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgttg                                                                                          <  2:3951833/140‑1 (MQ=255)

                                                 cGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTACAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTAGTTGGTa                                                                                       <  2:4233626/140‑1 (MQ=255)

                                                 cGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTa                                                                                       <  1:1145930/140‑1 (MQ=255)

                                                 cGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTa                                                                                       >  2:1746019/1‑140 (MQ=255)

                                                      aaaTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTa                                                                                  <  2:1026580/140‑1 (MQ=255)

                                                        aTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTCTTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACa                                                                                <  2:4142699/140‑1 (MQ=255)

                                                        aTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACa                                                                                <  1:2531760/140‑1 (MQ=255)

                                                        aTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACa                                                                                <  2:312195/140‑1 (MQ=255)

                                                        aTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACa                                                                                <  1:3981825/140‑1 (MQ=255)

                                                        aTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACa                                                                                >  1:4661425/1‑140 (MQ=255)

                                                        aTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACa                                                                                <  1:1707790/140‑1 (MQ=255)

                                                               aagaagaagCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCt                                                                         <  1:1034519/140‑1 (MQ=255)

                                                               aagaagaagCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCt                                                                         <  1:2896030/140‑1 (MQ=255)

                                                                  aagaagCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTaaa                                                                      <  2:2803717/140‑1 (MQ=255)

                                                                       gCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTc                                                                 <  2:3450154/140‑1 (MQ=255)

                                                                       gCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTc                                                                 <  2:2369992/140‑1 (MQ=255)

                                                                        ccAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTcc                                                                <  2:2093615/140‑1 (MQ=255)

                                                                           aaaaaGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGt                                                             >  1:3114615/1‑140 (MQ=255)

                                                                                ggCACTATTGCCTGGTCTATTCTCTCTTCGAATAATACGTCCGGAAATATTGATAAAATTAAAATTAAGTTCGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTAGTGTACAGACCGCTAAAGCGTCAGGTATGGa                                                        <  1:545034/140‑1 (MQ=255)

                                                                                 gCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGaa                                                       <  2:2694255/140‑1 (MQ=255)

                                                                                 gCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGaa                                                       >  1:3580843/1‑140 (MQ=255)

                                                                                     tATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGtt                                                   <  1:5325879/140‑1 (MQ=255)

                                                                                     tATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGtt                                                   <  1:445099/140‑1 (MQ=255)

                                                                                          ccTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATAAGGATAAAATTAAAATTAGGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTAGGGAACGTTTCCCg                                              >  2:4292511/1‑140 (MQ=255)

                                                                                            tGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCt                                            <  1:1467851/140‑1 (MQ=255)

                                                                                            tGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCt                                            <  1:2649318/140‑1 (MQ=255)

                                                                                            tGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCt                                            <  2:3629390/140‑1 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGcc                                         >  1:4054706/1‑140 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGcc                                         >  2:4389078/1‑140 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGcc                                         >  2:2910664/1‑140 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGcc                                         >  1:952517/1‑140 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGCAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTTGTATTGTACATACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGcc                                         >  2:5144733/1‑140 (MQ=255)

                                                                                                 tATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGt                                       >  1:1397745/1‑140 (MQ=255)

                                                                                                 tATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGt                                       <  1:2444930/140‑1 (MQ=255)

                                                                                                 tATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGt                                       <  1:2487127/140‑1 (MQ=255)

                                                                                                   ttCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTAt                                     >  1:2839761/1‑140 (MQ=255)

                                                                                                    tctctcTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTAtg                                    >  1:1807097/1‑140 (MQ=255)

                                                                                                        tctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGTTATGGAACGTTTCCCGCTGCCGTATGTGCt                                >  2:4399859/1‑140 (MQ=255)

                                                                                                        tctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTccc                                               >  2:266897/1‑125 (MQ=255)

                                                                                                        tctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCt                                >  2:869428/1‑140 (MQ=255)

                                                                                                        tctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCt                                >  1:3236468/1‑140 (MQ=255)

                                                                                                        tctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGATGCCGTATGTGCt                                >  1:4182183/1‑140 (MQ=255)

                                                                                                         ctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTg                               >  2:545287/1‑140 (MQ=255)

                                                                                                         ctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTg                               >  2:3460682/1‑140 (MQ=255)

                                                                                                           tCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGAc                             >  1:3315196/1‑140 (MQ=255)

                                                                                                            cGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGAcc                            >  2:1057821/1‑140 (MQ=255)

                                                                                                             gcgtaataCGTCCGGAAATATGGAGAAAGTTAAAATTAAGTCTGATTGATTAGCCTCCCACGGGATTACCTTTGTTGGTATTTTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGGATGTGCTGTCCa                           >  2:3366156/4‑140 (MQ=255)

                                                                                                             gCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCa                           >  1:3778924/1‑140 (MQ=255)

                                                                                                              cATAATACGTCCGGAAATATGGATACAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTCCAGACCGCTAAAGCGTCCGTTATGGAACGTTTCCCGCTGCCGTCTGTGCTGACCaa                          <  2:1822746/140‑1 (MQ=255)

                                                                                                               ataataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAAc                         <  2:1304230/140‑1 (MQ=255)

                                                                                                               ataataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAAc                         >  1:673665/1‑140 (MQ=255)

                                                                                                               ataataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAAc                         <  1:3013549/140‑1 (MQ=255)

                                                                                                               ataataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAAc                         <  1:435979/140‑1 (MQ=255)

                                                                                                                  ataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                            >  1:2138065/1‑70 (MQ=255)

                                                                                                                  ataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                            <  2:2138065/70‑1 (MQ=255)

                                                                                                                  ataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGc                      >  1:4405536/1‑140 (MQ=255)

                                                                                                                    aCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCa                    >  1:2172782/1‑140 (MQ=255)

                                                                                                                      gTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATa                  >  2:3834505/1‑140 (MQ=255)

                                                                                                                        ccGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAACCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCTCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAac                >  1:2959139/1‑140 (MQ=255)

                                                                                                                           gAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCa                                                                                                           >  2:3866153/1‑46 (MQ=255)

                                                                                                                           gAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCa                                                                                                           <  1:3866153/46‑1 (MQ=255)

                                                                                                                           gAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTGCCGCTGCCGTATGTGCTGACCAACTGCCATAactca             >  1:5276363/1‑140 (MQ=255)

                                                                                                                                  ggATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTgcg      <  2:1937045/140‑1 (MQ=255)

GCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGT  >  NZ_CP025268/801425‑801698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`