BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	833,226	T→C	378 (TAA→TAG)	ybhS ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	833,226	0	T	C	99.7%	1086.8 / ‑5.2	329	378 (TAA→TAG)	ybhS	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (142/185); total (142/187)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 329 total aligned reads displayed.

CGTGGCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACG  >  NZ_CP025268/833090‑833361

    gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCa                                                                                                                                  >  1:4185250/1‑140 (MQ=255)

    gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCa                                                                                                                                  >  1:5136550/1‑140 (MQ=255)

    gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCa                                                                                                                                  >  1:2409630/1‑140 (MQ=255)

    gcggcaAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCa                                                                                                                                  >  1:274601/1‑140 (MQ=255)

                       tCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAg                                                                                                               <  2:3547716/140‑1 (MQ=255)

                       tCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAg                                                                                                               <  1:14953/140‑1 (MQ=255)

                        cACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCGGCACCGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGc                                                                                                              <  1:3813622/140‑1 (MQ=255)

                        cACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGc                                                                                                              <  1:3958236/140‑1 (MQ=255)

                        cACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGc                                                                                                              >  1:4387949/1‑140 (MQ=255)

                        cACCTGAATGTGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGc                                                                                                              <  2:3924258/140‑1 (MQ=255)

                         aCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGcc                                                                                                             >  1:1104869/1‑140 (MQ=255)

                         aCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGcc                                                                                                             >  1:1745939/1‑140 (MQ=255)

                             gAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGt                                                                                                         >  2:4751038/1‑140 (MQ=255)

                             gAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGt                                                                                                         <  1:758158/140‑1 (MQ=255)

                               aTTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCa                                                                                                       >  2:1302252/1‑140 (MQ=255)

                               aTTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCa                                                                                                       >  2:3977104/1‑140 (MQ=255)

                               aTTAGCACGGGTAAAATCAGAATCGCGCGCGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCa                                                                                                       <  2:2485340/140‑1 (MQ=255)

                                 tAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAgg                                                                                                     <  2:1944021/140‑1 (MQ=255)

                                  aGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGc                                                                                                    <  1:2557800/140‑1 (MQ=255)

                                  aGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGc                                                                                                    <  1:2213247/140‑1 (MQ=255)

                                    cACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCg                                                                                                  <  1:2653357/140‑1 (MQ=255)

                                    cACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCGCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCg                                                                                                  >  1:1502913/1‑140 (MQ=255)

                                     aCGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGa                                                                                                 <  1:3904122/140‑1 (MQ=255)

                                       gggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATa                                                                                               <  1:4992704/140‑1 (MQ=255)

                                       gggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATa                                                                                               <  2:305349/140‑1 (MQ=255)

                                        ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGGAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaa                                                                                              >  2:4244863/1‑140 (MQ=255)

                                        ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTTCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCAACGTTTGTTTTTCAGCCACGTCAGTCCGATaa                                                                                              >  1:3022989/1‑140 (MQ=255)

                                        ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaa                                                                                              >  1:2493704/1‑140 (MQ=255)

                                        ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaa                                                                                              >  1:682764/1‑140 (MQ=255)

                                            aagtCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATCAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                          <  1:4444763/137‑1 (MQ=255)

                                            aaaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                          <  2:801622/140‑1 (MQ=255)

                                            aaaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                          <  2:3647866/140‑1 (MQ=255)

                                            aaaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                          <  1:3412151/140‑1 (MQ=255)

                                            aaaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                          <  1:2451172/140‑1 (MQ=255)

                                            aaaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                          <  1:1776985/140‑1 (MQ=255)

                                             aaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATc                                                                                         >  1:4223140/1‑140 (MQ=255)

                                              aTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCa                                                                                        >  2:4011867/1‑140 (MQ=255)

                                                   aaTCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCAccgcc                                                                                   >  1:950744/1‑140 (MQ=255)

                                                   aaTCGCGCGGGTTGGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCGCTTCTCCCTAATTCAGCCGACGTTTGGTTTTCAGCCACTTCAGGCCGATAAACATCAccgcc                                                                                   >  1:3081824/1‑140 (MQ=255)

                                                       gcgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                               <  1:1093006/140‑1 (MQ=255)

                                                       gcgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                               <  2:2587018/140‑1 (MQ=255)

                                                       gcgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                               <  1:4296441/140‑1 (MQ=255)

                                                       gcgcgGGTTTGCGGTTCCGGCAGCAACGACTGCACCTATTTGCGGATTAACGTCCATAAGCGATGAAACCTGCTCGGCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                               <  2:5155882/140‑1 (MQ=255)

                                                        cgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGc                                                                              >  2:1368794/1‑140 (MQ=255)

                                                        cgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGc                                                                              >  2:2994472/1‑140 (MQ=255)

                                                        cgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGc                                                                              >  2:3775818/1‑140 (MQ=255)

                                                            ggTTTGGTGTTCGCGCAGCAACGCCTTTAACTATGTGAGGATTAACGCCCAGAAGCGCTCATACATACTCGTCTCCAGCGTCCAGCCGATGTTTGGTTTACAGCCCAGTCACGCCGATAAACATCACCGCCGAAGCGATc                                                                          <  2:1657995/140‑1 (MQ=255)

                                                             gTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCa                                                                         <  1:126777/140‑1 (MQ=255)

                                                             gTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCa                                                                         >  2:3891360/1‑140 (MQ=255)

                                                                tGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCaaaa                                                                      >  2:4675242/1‑140 (MQ=255)

                                                                  cGGTTCGCGCAGCAACGCCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCACTTCTCCCTAATCCAGCCGGCGTTTGGTTTTCAGCACCGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAAc                                                                    <  2:1510654/140‑1 (MQ=255)

                                                                    gTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAg                                                                  <  1:4617074/140‑1 (MQ=255)

                                                                    gTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAg                                                                  <  1:129545/140‑1 (MQ=255)

                                                                     ttCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGc                                                                 <  1:676123/140‑1 (MQ=255)

                                                                     ttCGAGCAGCAAAGACTGAACCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGATGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAACCAGc                                                                 <  2:1761765/140‑1 (MQ=255)

                                                                      tCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                                <  2:3702111/140‑1 (MQ=255)

                                                                      tCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                                <  1:827713/140‑1 (MQ=255)

                                                                      tCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACCTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                                <  1:5277757/140‑1 (MQ=255)

                                                                       cgcgCAGCACCGACTGCAACTCTTTGCGGATTAACGTCGATAAGCGATGAAACATGCCCATCTTCCTAGTCCAGCTGACGCTTGGTTTTAAGCCACGTCAGGCCGGCAAACATCACCGCCGACGCGATCAAAAACAGcgc                                                               >  2:539547/1‑138 (MQ=255)

                                                                       cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCAc                                                               >  1:2590266/1‑140 (MQ=255)

                                                                       cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCAc                                                               >  1:4075333/1‑140 (MQ=255)

                                                                       cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCAc                                                               >  2:5225532/1‑140 (MQ=255)

                                                                       cgcgCACCCACGACCCCAACTCTTTGCGGATTAACGGCCATCAGCGCTGAAACATGCCCTTCCCCCTCATCCAGCCGACGTTTGTCTTTCAGCCGCGTCTGGCCGGCAAACATCACCGCCGACGCGATCAAAAACCGccc                                                               >  2:4418210/1‑138 (MQ=255)

                                                                           cagcaACGACTTAAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGttt                                                           <  1:3239755/140‑1 (MQ=255)

                                                                           cagcaACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCCGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGttt                                                           <  2:2946462/140‑1 (MQ=255)

                                                                                 cGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTaccacc                                                     <  2:2159490/140‑1 (MQ=255)

                                                                                   aCTGCACCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                                   <  2:3278934/140‑1 (MQ=255)

                                                                                   aCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                                   <  1:2850919/140‑1 (MQ=255)

                                                                                   aCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGAGCAAAAACAGCACGTTTACCACCAg                                                   >  1:296381/1‑140 (MQ=255)

                                                                                   aCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTGGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                                   >  1:3371819/1‑140 (MQ=255)

                                                                                    cTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGc                                                  >  1:1381500/1‑140 (MQ=255)

                                                                                     tGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGca                                                 <  2:3410263/140‑1 (MQ=255)

                                                                                      gCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcac                                                <  2:2659017/140‑1 (MQ=255)

                                                                                      gCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcac                                                <  1:2709204/140‑1 (MQ=255)

                                                                                      gCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGCTTGGTAGTCAGCCACGTCAGGACTAGACACCACCCCCCGGAAGCGATCAAAAACAGCATGCGTACCACCAGcac                                                <  2:2789551/140‑1 (MQ=255)

                                                                                        aaCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcacca                                              <  1:2350384/140‑1 (MQ=255)

                                                                                        aaCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcacca                                              <  1:4719594/140‑1 (MQ=255)

                                                                                          ctctTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACt                                            >  1:3527469/1‑140 (MQ=255)

                                                                                               tGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTAGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCTGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAat                                       >  2:3191432/1‑140 (MQ=255)

                                                                                                        aCGTCCATAAGCGATGAAACATGCTCTTCTCCCTATTCCAGCCGACGTTTGGTTTTCAGACACGTCAGGCCGATAATCATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGc                              <  2:1106703/140‑1 (MQ=255)

                                                                                                             cATAAGCGCTGAACCATGCTCTTCTCCCTAATCCAGACGCCGTTTGTTTTTCAGCCACCTAAGGCCGATAAAGATCTCCGTCGAAGCGATCAAAAACAGTACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGa                         <  2:742684/140‑1 (MQ=255)

                                                                                                              aTAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGaa                        >  2:777001/1‑140 (MQ=255)

                                                                                                               tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                       <  1:2639720/140‑1 (MQ=255)

                                                                                                               tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                       <  1:1027954/140‑1 (MQ=255)

                                                                                                               tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                       <  1:1772836/140‑1 (MQ=255)

                                                                                                               tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCCACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                       <  1:2727764/140‑1 (MQ=255)

                                                                                                                 aGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAg                     <  1:1425920/140‑1 (MQ=255)

                                                                                                                  gCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAgg                    <  1:2102930/140‑1 (MQ=255)

                                                                                                                  gCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAgg                    <  2:1447128/140‑1 (MQ=255)

                                                                                                                    gATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAGGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCt                  >  2:3315611/1‑140 (MQ=255)

                                                                                                                    gATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCt                  <  1:1645933/140‑1 (MQ=255)

                                                                                                                     aTGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGc                                                                 >  2:2201812/1‑92 (MQ=255)

                                                                                                                     aTGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcac                                                <  1:3656573/109‑1 (MQ=255)

                                                                                                                     aTGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCtt                 >  2:5030810/1‑140 (MQ=255)

                                                                                                                     aTGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGGTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCtt                 >  2:2320679/1‑140 (MQ=255)

                                                                                                                         aaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa             <  1:4881982/140‑1 (MQ=255)

                                                                                                                         aaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa             <  1:2033205/140‑1 (MQ=255)

                                                                                                                             tGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGt         >  1:1175090/1‑140 (MQ=255)

                                                                                                                                 cttctCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTg     <  1:3486199/140‑1 (MQ=255)

                                                                                                                                    ctcCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACg  >  2:1391021/1‑140 (MQ=255)

CGTGGCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACG  >  NZ_CP025268/833090‑833361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`