BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	879,908	A→G	304 (TAA→TAG)	gsiD →	glutathione ABC transporter permease GsiD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	879,908	0	A	G	100.0%	528.6 / NA	159	304 (TAA→TAG)	gsiD	glutathione ABC transporter permease GsiD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (52/107); total (52/107)

Only 100 of 159 total aligned reads displayed.

ATGAGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAG  >  NZ_CP025268/879772‑880044

aTGAGGCTCGAGCGGGTTTGGTTATCCCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGAGCGATCGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTa                                                                                                                                       <  2:375718/140‑1 (MQ=255)

aTGAGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTa                                                                                                                                       <  1:120506/140‑1 (MQ=255)

aTGAGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTa                                                                                                                                       <  1:1556581/140‑1 (MQ=255)

aTGAGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTa                                                                                                                                       <  1:1624483/140‑1 (MQ=255)

aTGAGGCTCGAGCGGATAAGGTTCTCGCGCCGCATGTCGCTGTTTTTCCGGCACTGGCTATTTTTCTGACCGTCCTGGCGTTCACTTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTa                                                                                                                                       <  2:375774/140‑1 (MQ=255)

  gAGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACg                                                                                                                                     <  1:1407916/140‑1 (MQ=255)

   aGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGt                                                                                                                                    <  1:3403534/140‑1 (MQ=255)

   aGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGt                                                                                                                                    <  1:3109241/140‑1 (MQ=255)

    ggCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGtt                                                                                                                                   >  1:59351/1‑140 (MQ=255)

     gCTCGAGCGGATATGGTTATCGCTGCGCATGTCGTTGTTTTTCCGGTCCGGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGGTCCGAAAATTAAATGATAGTTACGttt                                                                                                                                  <  2:2079018/140‑1 (MQ=255)

     gCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGttt                                                                                                                                  <  2:691392/140‑1 (MQ=255)

        cGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGaa                                                                                                                               <  1:2508600/140‑1 (MQ=255)

        cGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGaa                                                                                                                               <  1:2509091/140‑1 (MQ=255)

          aGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAata                                                                                                                             <  1:3361773/140‑1 (MQ=255)

           gCGGATCTGGTTATCGCGCCGCATGTCGTTGTTTTTCCGGCCCTGGCTATTTTTCTGGCCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTTGATCCGAAAATTAAAGGATAGTTACGTTTGAatat                                                                                                                            <  2:1350228/140‑1 (MQ=255)

           gCGGATATTTTTATCTCGCCGCGTGTCGCTTTTTTTCCACCCCTGGCTGTTTCTCTGACCGTACTGGCGTTCAACTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAatat                                                                                                                            <  2:4047548/140‑1 (MQ=255)

            cGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATAtt                                                                                                                           <  2:4541040/140‑1 (MQ=255)

             ggATATGGTTATCGCGCCGCATGTAGCTGTTTTTCCGGCGCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGTTCGGAAAATTAAAGGATAGTTACGTTTGAATATTg                                                                                                                          <  2:3462376/140‑1 (MQ=255)

              gATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGTGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGc                                                                                                                         >  2:2005885/1‑140 (MQ=255)

              gATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGc                                                                                                                         >  2:2907121/1‑140 (MQ=255)

                 atGGTTTTCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGTGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTCTGAATGTTGCGTg                                                                                                                      <  2:2147976/140‑1 (MQ=255)

                  tGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGGTGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGa                                                                                                                     <  2:596696/140‑1 (MQ=255)

                   ggTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGaa                                                                                                                    <  2:2386079/140‑1 (MQ=255)

                   ggTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTCGAATATTGCTTGaa                                                                                                                    <  2:2330221/140‑1 (MQ=255)

                    gTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGaaa                                                                                                                   <  1:3689515/140‑1 (MQ=255)

                         cgcgCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTa                                                                                                              >  1:608516/1‑140 (MQ=255)

                         cgcgCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTa                                                                                                              >  2:4840013/1‑140 (MQ=255)

                         cgcgCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTa                                                                                                              >  1:1068891/1‑140 (MQ=255)

                         cgcgCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTa                                                                                                              >  1:1984545/1‑140 (MQ=255)

                         cgcgCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTa                                                                                                              >  2:4883141/1‑140 (MQ=255)

                         cgcgCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTAAATATTGCGTGAAAGGGTa                                                                                                              >  2:1956798/1‑140 (MQ=255)

                           cgccgcATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAAt                                                                                                            <  1:2285434/140‑1 (MQ=255)

                           cgccgcATGTCGCCGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAAt                                                                                                            <  1:4703072/140‑1 (MQ=255)

                           cgccgcATCTCGCTGTTCTTCCCGCCCTGCTTATCTTTCTCCCCGTACTGGCTTTCAATTTGTTGAGAGATGGTTTACGCGATGCGCTAGATCCGAAAATTAAACTATAGTAACGTGTGAATATTGCTTCAAACGCTAAt                                                                                                            <  2:1895437/140‑1 (MQ=255)

                              cgcATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCAc                                                                                                         >  1:473317/1‑140 (MQ=255)

                                cATGTCGCTGTTTTTCCGCCACTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACCCGATGCGCTGGATCCGACAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCt                                                                                                       <  2:4563335/140‑1 (MQ=255)

                                  tGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTca                                                                                                     <  1:5108315/140‑1 (MQ=255)

                                  tGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTca                                                                                                     <  1:1897715/140‑1 (MQ=255)

                                  tGTCGCTGTTTTTCCGGACCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTca                                                                                                     <  2:1250455/140‑1 (MQ=255)

                                     cGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAg                                                                                                  <  1:898724/140‑1 (MQ=255)

                                       ctttttttCCGGCCCTGGCTATTTTTCTGACCGTACTGTCGTTCCATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGa                                                                                                <  1:947003/137‑1 (MQ=255)

                                       cTGTTTTTCCGGCTCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGa                                                                                                <  1:5252409/140‑1 (MQ=255)

                                       cTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGa                                                                                                <  1:3039859/140‑1 (MQ=255)

                                       cTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGa                                                                                                <  2:1247106/140‑1 (MQ=255)

                                       cTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGa                                                                                                <  1:849919/140‑1 (MQ=255)

                                            tttCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGTGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAatta                                                                                           <  1:3941036/140‑1 (MQ=255)

                                            tttCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAatta                                                                                           >  2:4764412/1‑140 (MQ=255)

                                            tttCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAatta                                                                                           <  1:3941065/140‑1 (MQ=255)

                                            tttCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAatta                                                                                           <  2:1839607/140‑1 (MQ=255)

                                            tttCCGGCCCGGGCGTTTTTTCTGACCGTACTGGCGTTCACTTTGTTGGGCGATGCTTTACGCGATGCGCTGGATCCCAAAATTGAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAACCACCTCACCGGAAatta                                                                                           <  2:2665744/140‑1 (MQ=255)

                                             ttCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAattat                                                                                          <  1:1092108/140‑1 (MQ=255)

                                             ttCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAattat                                                                                          <  1:3737376/140‑1 (MQ=255)

                                             ttCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAattat                                                                                          <  1:3150693/140‑1 (MQ=255)

                                                 ggCCCTTGCTATTTTTCTGCGCGTGCTGGCGTTCAATTTGTGGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGcc                                                                                      <  2:2743491/140‑1 (MQ=255)

                                                 ggCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGcc                                                                                      <  2:4356925/140‑1 (MQ=255)

                                                 ggCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGcc                                                                                      <  1:2347101/140‑1 (MQ=255)

                                                  gCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGccc                                                                                     <  1:4815417/140‑1 (MQ=255)

                                                    ccTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTa                                                                                   <  2:142631/140‑1 (MQ=255)

                                                       ggCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGc                                                                                <  1:74861/140‑1 (MQ=255)

                                                        gCTTTTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCa                                                                               <  2:4167718/140‑1 (MQ=255)

                                                        gCTCTTTTTCTGACCGTACTGGCGTTCAATGTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCa                                                                               <  2:4078703/140‑1 (MQ=255)

                                                        gCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGCTGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTACTCACCTCACAGGAAATTATTGCCCTAAGCa                                                                               <  2:95944/140‑1 (MQ=255)

                                                        gCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTTATCACCTCACAGGAAATTATTGCCCTAAGCa                                                                               <  2:2664661/140‑1 (MQ=255)

                                                        gCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCa                                                                               <  1:3549383/140‑1 (MQ=255)

                                                        gCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCa                                                                               <  1:4227216/140‑1 (MQ=255)

                                                        gCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCa                                                                               <  2:5208286/140‑1 (MQ=255)

                                                          tATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAg                                                                             <  1:5162834/140‑1 (MQ=255)

                                                             ttttCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAgtgt                                                                          >  1:1789506/1‑140 (MQ=255)

                                                               ttCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGtgttg                                                                        <  1:3870833/140‑1 (MQ=255)

                                                                  tGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTaa                                                                     >  1:534730/1‑140 (MQ=255)

                                                                      cGTACTGGCGCTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCAGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACttt                                                                 >  2:4918344/1‑140 (MQ=255)

                                                                       gTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGcc                                                                                      <  1:335406/118‑1 (MQ=255)

                                                                       gTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAAGTAAAGGATAGTTACGTGTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGcc                                                                                      >  2:335406/1‑118 (MQ=255)

                                                                         aCTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTa                                                                                                                                       <  2:1799683/67‑1 (MQ=255)

                                                                         aCTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTa                                                                                                                                       >  1:1799683/1‑67 (MQ=255)

                                                                          cTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTctgc                                                             <  1:2043252/140‑1 (MQ=255)

                                                                          cTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTctgc                                                             <  2:5223600/140‑1 (MQ=255)

                                                                             gCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGa                                                          <  2:4580099/140‑1 (MQ=255)

                                                                             gCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGa                                                          <  2:2419962/140‑1 (MQ=255)

                                                                                ttCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGa                                                                                                <  2:5057125/99‑1 (MQ=255)

                                                                                ttCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGa                                                                                                >  1:5057125/1‑99 (MQ=255)

                                                                                  cAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTTCTGATTTTg                                                     >  2:1523175/1‑140 (MQ=255)

                                                                                  cAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTg                                                     >  1:3654660/1‑140 (MQ=255)

                                                                                  cAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTg                                                     >  2:2015438/1‑140 (MQ=255)

                                                                                  cAATTCGTTGGGCGATGGTTTGCGCGATGCGCTGGATCCAACAATTAAAGGATAGTGGCGTTTGAATATTGCTTGAAAGGGTGATCACCTCACAGGAAATTATTGCCCTAAGCAGGTATTGTAACTTTATGCTGATGTTg                                                     >  2:1523176/1‑140 (MQ=255)

                                                                                  acaTTTGTTGGGCGATGGTTTCCGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTACTCCCCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTg                                                     <  2:3023391/138‑1 (MQ=255)

                                                                                    aTTTGTTGTGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTa                                                   <  2:1319410/140‑1 (MQ=255)

                                                                                       tgttgGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGaa                                                <  1:3444985/140‑1 (MQ=255)

                                                                                                  ggTTTACTCGTTGTGCTGGATCCGAAAATTAAAGGATAGTTTCGTTTGAATATTGATTGAAAGGGTACTCACCTCACAGGTACTTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAAttt                                     <  2:1087691/140‑1 (MQ=255)

                                                                                                             tGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGc                          >  1:4970785/1‑140 (MQ=255)

                                                                                                             tGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGc                          <  1:3623092/140‑1 (MQ=255)

                                                                                                             tGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGc                          >  1:4201820/1‑140 (MQ=255)

                                                                                                             tGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGc                          >  1:836279/1‑140 (MQ=255)

                                                                                                             tGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATAGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGc                          >  2:3163506/1‑140 (MQ=255)

                                                                                                                   ggATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCgcagc                    <  2:353308/140‑1 (MQ=255)

                                                                                                                    gATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCgcagca                   <  2:4402679/140‑1 (MQ=255)

                                                                                                                     aTCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCaa                  >  1:1320612/1‑140 (MQ=255)

                                                                                                                          aaaaTTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGAc             <  1:53950/140‑1 (MQ=255)

                                                                                                                                aaaGGATAGTTACGTTTTAATATTGGTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAAtttt       <  2:3541468/140‑1 (MQ=255)

                                                                                                                                  aGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTg     <  2:2627886/140‑1 (MQ=255)

ATGAGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAG  >  NZ_CP025268/879772‑880044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`