Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 75,278 G→A R12C (CGC→TGC)  thiB ← thiamine ABC transporter substrate binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP02526875,2780GA99.6% 850.7 / NA 229R12C (CGC→TGC) thiBthiamine ABC transporter substrate binding subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (134/94);  total (135/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

CAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGC  >  NZ_CP025268/75141‑75414
                                                                                                                                         |                                                                                                                                        
 aGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCacag                                                                                                                                       >  2:4413767/1‑140 (MQ=255)
  gcgcCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCacagc                                                                                                                                      <  2:5357020/140‑1 (MQ=255)
   cgcCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCacagca                                                                                                                                     >  2:3593742/1‑140 (MQ=255)
     ccaccaGTTTCAGTGCGCAATTACAGTCTGCGTCAAAGGCTTTTTTAACCACCGGACCGGGACCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGTTTTAGCGAAAACGGGCGCTGTGCGAAGGAAc                                                                                                                                   >  2:2779318/1‑140 (MQ=255)
       accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGGATCGTAGGTATAAACAGTCAGAACGGGGTTAGCGAAAACGGGCGCTGTGCACAGCAAcag                                                                                                                                 >  1:3686660/1‑140 (MQ=255)
       accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAAcag                                                                                                                                 >  1:2462191/1‑140 (MQ=255)
       accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAAcag                                                                                                                                 <  2:2189808/140‑1 (MQ=255)
       accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAAcag                                                                                                                                 >  2:510482/1‑140 (MQ=255)
       accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAAcag                                                                                                                                 >  2:89284/1‑140 (MQ=255)
       accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAAcaa                                                                                                                                 >  2:3659487/1‑139 (MQ=255)
        ccaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCTAGTCGGCGTCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAAcagc                                                                                                                                <  2:4723971/140‑1 (MQ=255)
           gTTTCCGTTCGCGATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAgg                                                                                                                             <  2:4748545/140‑1 (MQ=255)
                aGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAg                                                                                                                        >  1:4365053/1‑140 (MQ=255)
                  ttCGCAATTACAGTCGTCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGGGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGAc                                                                                                                      <  1:2956488/140‑1 (MQ=255)
                    cGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACAt                                                                                                                    >  1:4630494/1‑140 (MQ=255)
                              gTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAaca                                                                                                          <  1:2779212/140‑1 (MQ=255)
                                 ggCTTCAAAGGCTTTTTTAACCACCGGCCCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACtt                                                                                                       >  1:4333894/1‑140 (MQ=255)
                                 ggCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCTGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACtt                                                                                                       >  1:2667088/1‑140 (MQ=255)
                                 ggCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACtt                                                                                                       >  1:5004919/1‑140 (MQ=255)
                                 ggCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACtt                                                                                                       >  1:2629206/1‑140 (MQ=255)
                                   cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTg                                                                                                     <  1:5273040/140‑1 (MQ=255)
                                   cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTg                                                                                                     >  1:4834844/1‑140 (MQ=255)
                                   cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCAAGTCGGCGGGGAAGGATTCGTAGGTATAAACAGGCAGAACGGGGTGAGCGAAAACGGGGGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACttgt                                                                                                     >  2:199220/1‑138 (MQ=255)
                                   atacAAAGGATTTATTAACCAACGAACCAGGGACCCAGTCAGAGGAGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTCAGCGAATACGGGCGCTGTGCACAGCAACAGCAGGGGCAGAGATTTTTTTAACACTTTg                                                                                                     <  2:1618411/137‑1 (MQ=255)
                                     tCAAAGGCTTTTTGAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGACCGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTGGCa                                                                                                   <  1:1686611/140‑1 (MQ=255)
                                      cAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCAc                                                                                                  >  1:3505675/1‑140 (MQ=255)
                                           gCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGGATAAACAGTCAGAACGGGTTTAGCGAAAACGTGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCGCaa                                                                                             >  2:293662/1‑140 (MQ=255)
                                            cTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTTCACCTCaaa                                                                                            >  1:392409/1‑140 (MQ=255)
                                             ttttttAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCaaaa                                                                                           >  2:3430442/1‑140 (MQ=255)
                                             ttttttAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCaaaa                                                                                           >  2:4228821/1‑140 (MQ=255)
                                             ttttttAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCaaaa                                                                                           >  1:1557240/1‑140 (MQ=255)
                                                tttAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAag                                                                                        <  1:1435170/140‑1 (MQ=255)
                                                  tagccaccGGTCCAGGCCCCTAGTCGGGGCCGACGGCAGCGTAGATATAAACAGTCGGAAGGGGTTTAGCGAAAACGGGCGCTGTGCACTGCAGCAGCACGGGCAGACATTTTTTTCGCACTGTGCACCTCAAAAAagag                                                                                      <  2:2671333/137‑1 (MQ=255)
                                                  tAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAagag                                                                                      <  1:4703255/140‑1 (MQ=255)
                                                       cccGGACCAGGCCCACAGTCGGCGGCGAAGGAATCGTAGGTATCAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTTCACAGCAACAGCAGGGGCAGACATTTTTTTAACCCTTTGCACCTCAAAAAAGAGTGGcc                                                                                 <  2:3962335/139‑2 (MQ=255)
                                                       accGGACTAGGCCCCCAGGCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCa                                                                                 <  2:307565/140‑1 (MQ=255)
                                                       accGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCa                                                                                 >  1:3427413/1‑140 (MQ=255)
                                                       accGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCa                                                                                 <  1:2539392/140‑1 (MQ=255)
                                                        ccGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATACACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa                                                                                <  1:1002203/140‑1 (MQ=255)
                                                        ccGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa                                                                                <  2:3759512/140‑1 (MQ=255)
                                                        ccGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa                                                                                <  2:3420834/140‑1 (MQ=255)
                                                           gACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAgg                                                                             <  1:4775152/140‑1 (MQ=255)
                                                           gACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAgg                                                                             <  2:2463664/140‑1 (MQ=255)
                                                           gACCAGGCCCCCAGTCAGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTATCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAgg                                                                             <  1:2952328/140‑1 (MQ=255)
                                                             ccAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGAc                                                                           <  2:5010145/140‑1 (MQ=255)
                                                             ccAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGAc                                                                           <  2:760348/140‑1 (MQ=255)
                                                                ggCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGCACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGCGGCAAAGGACTTg                                                                        >  2:392349/1‑140 (MQ=255)
                                                                ggCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTg                                                                        >  1:3027107/1‑140 (MQ=255)
                                                                ggCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTg                                                                        >  2:4507173/1‑140 (MQ=255)
                                                                 gCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTga                                                                       >  1:4858244/1‑140 (MQ=255)
                                                                   ccccAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTgaga                                                                     >  1:4013624/1‑140 (MQ=255)
                                                                    cccAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGaa                                                                    <  2:2347609/140‑1 (MQ=255)
                                                                     ccAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAg                                                                   <  1:499429/140‑1 (MQ=255)
                                                                      cAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAgg                                                                  >  1:3662668/1‑140 (MQ=255)
                                                                      cAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAgg                                                                  >  2:1119254/1‑140 (MQ=255)
                                                                      cAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAgg                                                                  >  1:4526379/1‑140 (MQ=255)
                                                                      cAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGAAGAAATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAgg                                                                  >  1:3813963/1‑140 (MQ=255)
                                                                          cggcggCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGcc                                                              >  2:134732/1‑140 (MQ=255)
                                                                           ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCt                                                             >  1:4840649/1‑140 (MQ=255)
                                                                                cgAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAAt                                                        <  1:5167588/140‑1 (MQ=255)
                                                                                        tCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGc                                                <  1:3384902/140‑1 (MQ=255)
                                                                                        tCGTAGGTATAAACAGTCAGAACGGGTTTAGCCAACTCGGGCGCTGTGCACACCCACAGAAGGGGCAGAAATTTTTTTACCCCTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGACGGAGCCTCAACTCCCTTCGc                                                <  1:3158814/140‑1 (MQ=255)
                                                                                             ggTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCg                                           <  2:492929/140‑1 (MQ=255)
                                                                                               tataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCgccgac                                         >  1:4948275/1‑135 (MQ=255)
                                                                                               tataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGtt                                         >  1:1612386/1‑140 (MQ=255)
                                                                                                ataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTa                                        >  1:4586413/1‑140 (MQ=255)
                                                                                                  aaaCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATc                                      >  2:563737/1‑140 (MQ=255)
                                                                                                  aaaCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATc                                      <  1:1222956/140‑1 (MQ=255)
                                                                                                  aaaCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATc                                      <  1:1168914/140‑1 (MQ=255)
                                                                                                  aaaCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATc                                      <  1:783111/140‑1 (MQ=255)
                                                                                                    aCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCg                                    >  2:4623794/1‑140 (MQ=255)
                                                                                                      aGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGa                                  >  1:840951/1‑140 (MQ=255)
                                                                                                        tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCg                                           <  2:790476/129‑1 (MQ=255)
                                                                                                        tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  1:3286712/1‑140 (MQ=255)
                                                                                                        tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  1:3700470/1‑140 (MQ=255)
                                                                                                        tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  1:3779716/1‑140 (MQ=255)
                                                                                                        tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  2:5229932/1‑140 (MQ=255)
                                                                                                        tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  2:405726/1‑140 (MQ=255)
                                                                                                        tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  1:218429/1‑140 (MQ=255)
                                                                                                        gcAGAACGGGTTTAGCGAAAAGGGGCGCTGTGCACAGCAACTGCAGGGGCAGACATTTTTGTAACAATTTGCACCTAAAAAAAGAGTGGCAAAGGACTTCAGAAGGACCCTGAAATCCCTTCGGCGGCGTTATCCGGATc                                >  2:4432617/2‑140 (MQ=255)
                                                                                                            aaCGGGTTTAGCGAAAACGGGCGCTGTTCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGt                            >  1:145478/1‑140 (MQ=255)
                                                                                                            aaCGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCACTTCGCCGGCGTTACCCGGATCAGGt                            >  2:140937/1‑140 (MQ=255)
                                                                                                             aCGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGtt                           >  1:2722202/1‑140 (MQ=255)
                                                                                                                ggTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGa                        <  2:1685193/140‑1 (MQ=255)
                                                                                                                ggTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGa                        <  1:2343945/140‑1 (MQ=255)
                                                                                                                 gTTTAGCGAAAACGGGCGCTTTGCACAGCAACAGCATGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGTACTTGAGAAGGAGCCTCAAAGCCCTACGCCGGCGGTATCCGGATCAGGTTCGAc                       >  2:2056106/1‑140 (MQ=255)
                                                                                                                 gTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGAc                       >  2:262376/1‑140 (MQ=255)
                                                                                                                  tttAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACg                      <  1:4713292/140‑1 (MQ=255)
                                                                                                                    tAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACggg                    >  1:422292/1‑140 (MQ=255)
                                                                                                                      gCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGc                                                <  2:261126/110‑1 (MQ=255)
                                                                                                                           aaCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACCCTTTGCACCTCAAAAAAGATTGGCAAAGGACTTGAGAAGGAGGCTCAAATCCCTTCGCCGGCGTTATTCGGATCAGGTTCGACGGGTATTTtc             >  2:2491081/1‑140 (MQ=255)
                                                                                                                           aaCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTtc             >  2:2304911/1‑140 (MQ=255)
                                                                                                                           aaCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTtc             >  1:4800217/1‑140 (MQ=255)
                                                                                                                            aCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTtct            <  1:563737/140‑1 (MQ=255)
                                                                                                                            aCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTtct            >  1:1855755/1‑140 (MQ=255)
                                                                                                                                 cgcTGTGCACAGCAACCGCAGGGGCAGACATTTTTTTCACACGTTGCCCCTCAAAAAAGAGTGCACAAGGACTTGAGAAGGCGCCTCAGCTCCCTTCGCCGCCGTTATCCGGATTAGGTTCGACGGGTATTTTCTCAgcg       <  2:5133203/140‑1 (MQ=255)
                                                                                                                                 cgcTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAgcg       <  2:2462191/140‑1 (MQ=255)
                                                                                                                                 cgcTGTGCACAGCAACAGCAGGGACAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAGATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAgcg       <  2:4147298/140‑1 (MQ=255)
                                                                                                                                    tgtgCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCac    >  2:146903/1‑140 (MQ=255)
                                                                                                                                      tgCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCacgc  <  1:510482/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGC  >  NZ_CP025268/75141‑75414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑