BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	912,850	A→G	277 (TAA→TAG) R337R (CGT→CGC)	amiD → ybjS ←	N‑acetylmuramoyl‑L‑alanine amidase AmiD NAD(P)‑dependent oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	912,850	0	A	G	100.0%	1274.9 / NA	374	277 (TAA→TAG) R337R (CGT→CGC)	amiD ybjS	N‑acetylmuramoyl‑L‑alanine amidase AmiD NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (197/177); total (197/177)

Only 100 of 379 total aligned reads displayed.

GATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGA  >  NZ_CP025268/912713‑912984

 atatGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCGGGAGAAATACGGGCAGGATTAgcgc                                                                                                                                     >  2:4230550/1‑140 (MQ=255)

      accccGCGCGGGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACCGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGAGTAGCGCGGCAg                                                                                                                                <  1:1383773/137‑1 (MQ=255)

      acacCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAg                                                                                                                                >  2:3850206/1‑140 (MQ=255)

       cacCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGAGTAGCGCGGCAGt                                                                                                                               >  1:4576802/1‑140 (MQ=255)

          cgcgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGtttt                                                                                                                            >  2:2639920/1‑140 (MQ=255)

          cgcgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGtttt                                                                                                                            >  2:3503085/1‑140 (MQ=255)

          cgcgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGtttt                                                                                                                            >  1:1053793/1‑140 (MQ=255)

           gcgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTc                                                                                                                           >  1:2804830/1‑140 (MQ=255)

           gcgcgAGCAGCGGCGCGTGATAATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAAGGGCGAGGCGGATGCACAAACTCAGGCGATTGCCGAAGCTTTGCTGGTGAATTACGGGCAGGAGTAGCGCGGTAGTTTTc                                                                                                                           >  2:4429773/1‑140 (MQ=255)

            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATACCGGCGAAGCGGATGCAGAACCTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                          >  2:1711202/1‑140 (MQ=255)

            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                          >  2:1030332/1‑140 (MQ=255)

            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                          >  2:574337/1‑140 (MQ=255)

            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                          >  2:5152552/1‑140 (MQ=255)

            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                          >  1:1118710/1‑140 (MQ=255)

            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                          >  1:3614085/1‑140 (MQ=255)

            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                          >  1:3091085/1‑140 (MQ=255)

            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGAGGCAGAAACTCAGGCGAGTGCCGAAGCATTGCTGGAGCAATACGGGCAGGATTAGCGCGGCAGGTTTcc                                                                                                                          >  1:3015753/1‑140 (MQ=255)

              cgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGt                                                                                                                        <  2:149002/140‑1 (MQ=255)

                  cagcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTc                                                                                                                    <  1:2725748/140‑1 (MQ=255)

                   agcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTcg                                                                                                                   <  1:5116071/140‑1 (MQ=255)

                    gcggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTcgc                                                                                                                  <  1:3683334/140‑1 (MQ=255)

                         gcgTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGc                                                                                                             <  1:80826/140‑1 (MQ=255)

                          cgTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGcc                                                                                                            >  1:108529/1‑140 (MQ=255)

                           gTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTcgcgctaatc                                                                                                           <  1:1164245/140‑7 (MQ=255)

                                  tGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCATAGCCAggcggcg                                                                                                    >  2:4631571/1‑139 (MQ=255)

                                    gCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGTTTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGt                                                                                                  <  2:1920542/140‑1 (MQ=255)

                                       ttCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAAAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTGGCCAGGCGGCAGtttt                                                                                               >  1:2948266/1‑140 (MQ=255)

                                        tCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGGGGCAGTTTtc                                                                                              >  1:4853539/1‑140 (MQ=255)

                                         ccAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtct                                                                                             >  1:2486437/1‑140 (MQ=255)

                                           aGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCg                                                                                           <  1:5275176/140‑1 (MQ=255)

                                            gATGCATTTCCGGCCGACGTTATATAACGGCGGAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                          <  2:5255229/140‑1 (MQ=255)

                                            gATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGTCAGTTTTCTCGa                                                                                          <  2:5286007/140‑1 (MQ=255)

                                            gATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                          <  1:375768/140‑1 (MQ=255)

                                              tGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATa                                                                                        >  2:4010156/1‑140 (MQ=255)

                                                cATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                                                      <  1:2137472/140‑1 (MQ=255)

                                                cATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                                                      <  2:406089/140‑1 (MQ=255)

                                                cATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                                                      <  2:2814875/140‑1 (MQ=255)

                                                cATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                                                      <  2:3696166/140‑1 (MQ=255)

                                                 aTTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCt                                                                                     <  1:2370999/140‑1 (MQ=255)

                                                  tttCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCtt                                                                                    >  2:2766651/1‑140 (MQ=255)

                                                  tttCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCtt                                                                                    >  1:3387094/1‑140 (MQ=255)

                                                       gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                                                                               <  1:4064651/140‑1 (MQ=255)

                                                       gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                                                                               <  1:2105458/140‑1 (MQ=255)

                                                       gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                                                                               <  2:2965795/140‑1 (MQ=255)

                                                        ccccACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCCCTTGCCGAAGCATTGCTGGAGACATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTCGCCAGGCGGCAGTTTTCTCGATACCTTCGTCCa                                                                              <  2:1111772/140‑1 (MQ=255)

                                                        cccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCa                                                                              <  2:4886969/140‑1 (MQ=255)

                                                        cccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCa                                                                              <  1:1599359/140‑1 (MQ=255)

                                                                  tataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGAc                                                                    <  1:174649/140‑1 (MQ=255)

                                                                       cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCt                                                               >  1:2416580/1‑140 (MQ=255)

                                                                       cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCt                                                               >  2:2241215/1‑140 (MQ=255)

                                                                       cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCt                                                               >  2:3355115/1‑140 (MQ=255)

                                                                       cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGCAGATGACCCGCt                                                               >  2:1600413/1‑140 (MQ=255)

                                                                       cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACC‑TCATCCAGGGTGATGACCGGCTg                                                              >  1:4486665/1‑140 (MQ=255)

                                                                         gCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGAc                                                                    <  1:3539996/133‑1 (MQ=255)

                                                                         gCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                             <  2:701295/140‑1 (MQ=255)

                                                                         gCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                             <  1:2551748/140‑1 (MQ=255)

                                                                         gCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                             <  1:2316158/140‑1 (MQ=255)

                                                                         gCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                             >  2:4930685/1‑140 (MQ=255)

                                                                         gCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                             >  1:4321421/1‑140 (MQ=255)

                                                                         gCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                             <  1:4191834/140‑1 (MQ=255)

                                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt                                                            >  2:1174742/1‑140 (MQ=255)

                                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt                                                            >  2:3426281/1‑140 (MQ=255)

                                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt                                                            >  1:1832037/1‑140 (MQ=255)

                                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt                                                            >  2:478943/1‑140 (MQ=255)

                                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt                                                            >  1:2023678/1‑140 (MQ=255)

                                                                           gAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATa                                                           >  1:726254/1‑140 (MQ=255)

                                                                            aaGCGGATGCAGAAACTCAGGCGATTGCCGATGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGGGATGACCGGCTGATaa                                                          >  2:4095036/1‑140 (MQ=255)

                                                                            aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGGAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATaa                                                          >  2:4370152/1‑140 (MQ=255)

                                                                            aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATaa                                                          >  1:2493582/1‑140 (MQ=255)

                                                                             aGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAAc                                                         <  1:187093/140‑1 (MQ=255)

                                                                                 gATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTaa                                                     >  1:4367170/1‑140 (MQ=255)

                                                                                          aCTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTg                                            <  1:3303849/140‑1 (MQ=255)

                                                                                            tCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTgcg                                          <  2:5090148/140‑1 (MQ=255)

                                                                                            tCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTgcg                                          <  2:395416/140‑1 (MQ=255)

                                                                                              aGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGAGGCAGTTTTCCCTAGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCa                                        <  1:4429773/140‑1 (MQ=255)

                                                                                               ggCGCTCGCCGAAGCATGGCTGGAGAAATACGGGCAAGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGGGATGACTGGCTGATAACCTATCTCTTCCGGCGCAc                                       <  2:3209043/140‑1 (MQ=255)

                                                                                               ggCGATTGGCGAAGGCTTGCTGGAGAAATCCGGGCAGGATTAGCGCGGCCGTTTTCCGTGGTCGCGTGGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTACCTCTTCCTGCGCAc                                       <  2:3030033/140‑1 (MQ=255)

                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       <  1:1498669/140‑1 (MQ=255)

                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       <  1:1666120/140‑1 (MQ=255)

                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       <  2:2471062/140‑1 (MQ=255)

                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       <  2:4519441/140‑1 (MQ=255)

                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       <  1:3880672/140‑1 (MQ=255)

                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       <  2:62165/140‑1 (MQ=255)

                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       <  1:1341839/140‑1 (MQ=255)

                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       <  1:669137/140‑1 (MQ=255)

                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       >  1:752637/1‑140 (MQ=255)

                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       <  1:893885/140‑1 (MQ=255)

                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       <  2:1365835/140‑1 (MQ=255)

                                                                                               ggCGATTGCCGAAGAATTGCTGGAGAAATACGGGCATGATGAGCGCGGCAGCTTTCCGTGGTCGCGTAGCCAGGCGGCAATTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCGGCGCAc                                       <  2:1971921/140‑1 (MQ=255)

                                                                                                    ttGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTa                                  <  1:4620878/140‑1 (MQ=255)

                                                                                                    ttGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTa                                  <  2:3299620/140‑1 (MQ=255)

                                                                                                         gAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATc                             <  1:186495/140‑1 (MQ=255)

                                                                                                               ttGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGt                       <  1:288980/140‑1 (MQ=255)

                                                                                                                 gCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTaa                     >  2:2924766/1‑140 (MQ=255)

                                                                                                                       gaAGTACGGGCAGGATTAGCGCGGCGGGTTTCCGTGGTGGCGTAGCCAGGCGGCGGCTTTTTCGATACCTTCATCCTGGGTGAGGACCGGCTTATAACTCAACTTTTCCTGCGGACGCGTAATATCCAGCGTAAAGTCaa               >  2:806202/1‑140 (MQ=255)

                                                                                                                       gaAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaa               >  1:4941177/1‑140 (MQ=255)

                                                                                                                          aTACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAAt            >  1:556005/1‑140 (MQ=255)

                                                                                                                           tACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAAtt           <  1:2241215/140‑1 (MQ=255)

                                                                                                                               ggCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCATTTTTCTCGATACCTTCATCCAGGGTGATGACGGGCTGATCACCTAACTCTTCCTGCGCACTCGTAATATCCAGCGTAAGGTCAAAATTCAAc       >  2:214574/1‑140 (MQ=255)

                                                                                                                               ggCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCAAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAAc       >  2:2511350/1‑140 (MQ=255)

GATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGA  >  NZ_CP025268/912713‑912984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`