BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	920,456	A→G	L156L (TTG→CTG)	hcp ←	hydroxylamine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	920,456	0	A	G	98.8%	852.7 / NA	260	L156L (TTG→CTG)	hcp	hydroxylamine reductase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (171/85); minor base T (1/2); total (172/88)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

AAGCGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACG  >  NZ_CP025268/920322‑920592

   cgcgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGGACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGTCCGCCGCCCCTTTCAGGCCAGACAGGGAcagct                                                                                                                                  >  2:328330/1‑139 (MQ=255)

     cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGa                                                                                                                                >  2:660732/1‑140 (MQ=255)

     cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGa                                                                                                                                >  2:3335442/1‑140 (MQ=255)

     cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGa                                                                                                                                >  1:50544/1‑140 (MQ=255)

     cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGa                                                                                                                                >  2:12140/1‑140 (MQ=255)

     cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGa                                                                                                                                >  1:726200/1‑140 (MQ=255)

     cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCAGCACCTTTCAGGCCATACAGGCACAGCAGa                                                                                                                                >  2:3779943/1‑140 (MQ=255)

       ttCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACg                                                                                                                              <  1:134450/140‑1 (MQ=255)

       ttCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACg                                                                                                                              >  1:4227141/1‑140 (MQ=255)

       tgcATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGTCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACg                                                                                                                              >  1:4282271/3‑140 (MQ=255)

                gCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCgag                                                                                                                     >  1:378896/1‑140 (MQ=255)

                gCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCgag                                                                                                                     >  1:4747791/1‑140 (MQ=255)

                    gCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATg                                                                                                                 >  1:3353770/1‑140 (MQ=255)

                      cAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGtt                                                                                                               >  1:1460972/1‑140 (MQ=255)

                              cAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCaa                                                                                                       >  2:758730/1‑140 (MQ=255)

                                 ccACGCCATGATTTTATGCTACTGGGCATAAATATCGTTGTCGTATTGACCGAGCACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATCCAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcg                                                                                                    <  2:4465528/140‑1 (MQ=255)

                                    cGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcg                                                                                                    >  2:4100083/1‑137 (MQ=255)

                                       caagATGTTATGGCACTGGGTATAAATATAGTTGTCGTATCGACCGAGAACATGCGCGTGCTCCATATAGGCCGCTTCACCCTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCtt                                                                                              <  2:2043982/137‑1 (MQ=255)

                                       cATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCtt                                                                                              >  1:3110944/1‑140 (MQ=255)

                                                atcctACTGCCCACAAATATTGTTCTCGTATAGCCCCATAGCCTGCGTCTGTTCTATATAGGCCGCCGCACCTTTCATGCTCTCCATCCCCCGCTGATGTAGCCCGATAATTTTCTCGCCAATCTCCGCCTTATCTTTGt                                                                                     <  2:5135536/136‑1 (MQ=255)

                                                aTGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt                                                                                     <  1:950677/140‑1 (MQ=255)

                                                aTGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt                                                                                     >  2:493439/1‑140 (MQ=255)

                                                     aCTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAggg                                                                                <  1:4307362/140‑1 (MQ=255)

                                                      cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAgggg                                                                               >  1:2994440/1‑140 (MQ=255)

                                                       tGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGt                                                                              <  1:1113516/140‑1 (MQ=255)

                                                         ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                                            >  2:1371751/1‑140 (MQ=255)

                                                         ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                                            >  2:3037506/1‑140 (MQ=255)

                                                         ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                                            >  1:1331311/1‑140 (MQ=255)

                                                         ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                                            >  1:3949442/1‑140 (MQ=255)

                                                          gCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaaa                                                                           <  1:4877098/140‑1 (MQ=255)

                                                              aaaTCTCGTTGTCGTACCGACCGAGAACTTGCGCGTGTTCCATATAGGACGCCGCCCCTTGCAGGCCCTACAGGCCCCGCAGGCGCCGGCCGAGACTGTTTTCGCCACTCGCGGCTTTATCTCTGTCAGGCGTagagtgt                                                                       >  2:406768/1‑134 (MQ=255)

                                                              aaaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct                                                                       >  1:480921/1‑140 (MQ=255)

                                                              aaaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGGTTTCGCCAATCGCCGCCTTATCTGTGTCAGGGGGAAATTct                                                                       >  2:261610/1‑140 (MQ=255)

                                                                    cGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGcc                                                                 >  2:2221916/1‑140 (MQ=255)

                                                                    cGTTGTCGTATTGACCGAGAACATGCGCGGGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCCGGCCGAGAATGTTTTCGCCAATCGCCGCTTAATCGTTGTTAGTGGTAAATTCTGCTGcc                                                                 >  2:251521/1‑140 (MQ=255)

                                                                      ttGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTg                                                               <  1:4906497/140‑1 (MQ=255)

                                                                        gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGAc                                                             >  1:669667/1‑140 (MQ=255)

                                                                        gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGAc                                                             >  1:3822618/1‑140 (MQ=255)

                                                                         tCGTTTTGCCCTAGTACATGCTCGTGTTACAAATAGACCGCCGCACCATGCAGAGAAAATCGGAACAGCAGAAGCAGGCCGAGAATATCTCGGCCAACGGCCGCTCTTTCATTGATAGGGGTAAATTCTGCTGCCTGACg                                                            >  2:1032238/1‑140 (MQ=255)

                                                                            tATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAGTTCTGCTGCCTGACGTTg                                                         >  2:257315/1‑140 (MQ=255)

                                                                            tATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTg                                                         >  2:1880154/1‑140 (MQ=255)

                                                                              ttGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCa                                                       <  1:1908356/140‑1 (MQ=255)

                                                                               tGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAg                                                      <  1:4545205/140‑1 (MQ=255)

                                                                                 aCCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCt                                                    >  1:3689417/1‑140 (MQ=255)

                                                                                   cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGCCGTTGCAGCTcc                                                  >  2:2746653/1‑139 (MQ=255)

                                                                                   cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCg                                                  >  1:1209830/1‑140 (MQ=255)

                                                                                   cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCg                                                  >  2:1642096/1‑140 (MQ=255)

                                                                                   cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACATTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCCTTATCTTTGTTAGGGGTAAATTCTGCTGCCTAACGTTGCAGCTCg                                                  >  2:4623576/1‑140 (MQ=255)

                                                                                          ttccgcgTGTTCCATATAGGCCGCCGCACCTTTCCGCCCATACAGGCACAGCAGACGCAGGCCGAGGATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAt                                           <  1:1642751/137‑1 (MQ=255)

                                                                                          aTGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAt                                           <  1:895471/140‑1 (MQ=255)

                                                                                          aTGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAt                                           <  1:4007475/140‑1 (MQ=255)

                                                                                               cgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAtcgtcg                                      <  1:2229858/140‑1 (MQ=255)

                                                                                                   ttCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCa                                  <  2:3624270/140‑1 (MQ=255)

                                                                                                   ttCCATATAGGACGCCGCACCTTCCAGGCCATACAGGCACAGCAGACGCAGGCAGAGAACGTTTTCGCCAATCGCCGCTTTATCATTGTTAGGGGGAAATTCTGCTGCCTCACGTTGAAGCTCGCTGAGATAGTCGCTCa                                  <  2:1517988/140‑1 (MQ=255)

                                                                                                    tCCATATAGGCCGCCGCACCTTTCAGGCCATACATGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                                 <  2:2207514/140‑1 (MQ=255)

                                                                                                    tCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                                 <  2:5067545/140‑1 (MQ=255)

                                                                                                    tCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                                 >  2:3137937/1‑140 (MQ=255)

                                                                                                    tCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                                 <  2:4354431/140‑1 (MQ=255)

                                                                                                      cATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCa                               >  1:1578948/1‑140 (MQ=255)

                                                                                                       atatAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAg                              <  1:343935/140‑1 (MQ=255)

                                                                                                       atatAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAg                              >  2:555086/1‑140 (MQ=255)

                                                                                                        tataGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGt                             >  2:914871/1‑140 (MQ=255)

                                                                                                        tataGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGt                             >  2:2460815/1‑140 (MQ=255)

                                                                                                         ataGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGtt                            <  1:3653560/140‑1 (MQ=255)

                                                                                                          taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTg                           <  1:2849212/140‑1 (MQ=255)

                                                                                                           aGGCCGCCGCACCTTCCACGCCATCCAGGCACAGCACACTCACGCCGAGACTGTTATAGCAAATCGCCGCTTTATCTTTGTTAGGCGTCAATTCTGCTGACTCACGTTGCAGCTCGCACAGATCGCCGCTCACCAGTTGc                          <  2:1483962/140‑1 (MQ=255)

                                                                                                            ggCCGCCGCACCTTTCAGGCCATGCAGGCACAGCAGACGCAGGCCGAGAATGGTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCa                         >  1:2423816/1‑140 (MQ=255)

                                                                                                             gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAGTGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGCGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCTCCAGTTGCAg                        >  2:3930799/1‑140 (MQ=255)

                                                                                                             gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  2:3668405/1‑140 (MQ=255)

                                                                                                             gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  1:313035/1‑140 (MQ=255)

                                                                                                             gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  2:2710076/1‑140 (MQ=255)

                                                                                                             gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  2:4397813/1‑140 (MQ=255)

                                                                                                             gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  1:5224924/1‑140 (MQ=255)

                                                                                                             gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTCGCAg                        >  2:4816177/1‑140 (MQ=255)

                                                                                                             gccgccGCACCTTTCAGGCCAGACATGCACAGCAGACGCAGGCCGATAATTTTTTCGCCAAGCGCCTCTTTATCGTTGATAGGGTTAAATTCTTCTGCCTTACTTTGCAGCTCGCCGAGATCTTCTCTCACCAGTTGCAg                        >  2:4057893/1‑140 (MQ=255)

                                                                                                               cgccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGt                      <  1:4397813/140‑1 (MQ=255)

                                                                                                               cgccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGt                      <  1:2025578/140‑1 (MQ=255)

                                                                                                               cgccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGt                      >  2:2632004/1‑140 (MQ=255)

                                                                                                                 ccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCa                    >  1:2088736/1‑140 (MQ=255)

                                                                                                                  cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  2:3381781/1‑140 (MQ=255)

                                                                                                                  cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  1:2750753/1‑140 (MQ=255)

                                                                                                                  cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  1:4179847/1‑140 (MQ=255)

                                                                                                                  cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  1:16644/1‑140 (MQ=255)

                                                                                                                  cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  1:4702698/1‑140 (MQ=255)

                                                                                                                  cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  2:3703484/1‑140 (MQ=255)

                                                                                                                  cgcACCTTTCAGGACATACAGGCACAGCAGACGCAGCCCGAGAATGTTTTAGCCAAGCGCCCCTTGAGCTTTGTTGGGGGTAAATTCTGCTGCCTGACGGTGCAGCTCGCCGAGATCGTCGCTCACCAGTCGCAGGGCAg                   >  2:5242684/1‑140 (MQ=255)

                                                                                                                      ccTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt               <  1:1030505/140‑1 (MQ=255)

                                                                                                                      ccTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt               <  2:2876356/140‑1 (MQ=255)

                                                                                                                      ccTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt               <  2:4252625/140‑1 (MQ=255)

                                                                                                                      ccTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt               <  1:4899259/140‑1 (MQ=255)

                                                                                                                      ccTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt               <  1:959482/140‑1 (MQ=255)

                                                                                                                      ccTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt               <  1:2682463/140‑1 (MQ=255)

                                                                                                                      ccTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt               <  1:3882555/140‑1 (MQ=255)

                                                                                                                      ccTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt               <  2:641991/140‑1 (MQ=255)

                                                                                                                        tttCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCg             <  1:3337600/140‑1 (MQ=255)

                                                                                                                         ttCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCgg            >  1:2557805/1‑140 (MQ=255)

                                                                                                                                 aTACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGa    >  2:144242/1‑140 (MQ=255)

                                                                                                                                 aTACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGa    >  1:559399/1‑140 (MQ=255)

                                                                                                                                 aTACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGa    >  1:2835169/1‑140 (MQ=255)

                                                                                                                                   aCAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCACTTGCAGGTCAGCCATCGGGTTATCGAcg  >  2:1328392/1‑140 (MQ=255)

AAGCGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACG  >  NZ_CP025268/920322‑920592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`