BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	973,690	A→G	755 (TAA→TAG)	ycaI →	ComEC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	973,690	0	A	G	99.0%	994.5 / ‑5.0	303	755 (TAA→TAG)	ycaI	ComEC family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (175/124); minor base C (2/1); total (178/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

ATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGT  >  NZ_CP025268/973555‑973826

    aTTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTTGATAACGGGTAGAATATGCg                                                                                                                                  <  2:1784146/140‑1 (MQ=255)

    aTTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCg                                                                                                                                  >  1:4060419/1‑140 (MQ=255)

    aTTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCg                                                                                                                                  >  1:170997/1‑140 (MQ=255)

     ttGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCgg                                                                                                                                 <  1:3739819/140‑1 (MQ=255)

     ttGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCgg                                                                                                                                 >  1:4343433/1‑140 (MQ=255)

      tGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGc                                                                                                                                <  1:5157246/140‑1 (MQ=255)

      tGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGc                                                                                                                                >  2:1713546/1‑140 (MQ=255)

        aTACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTa                                                                                                                              >  1:1051621/1‑140 (MQ=255)

        aTACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTa                                                                                                                              >  2:3766094/1‑140 (MQ=255)

        aTACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTCGTTTGGCGTGCCAGTGGATAACAGGTAGAATATGCGGCTa                                                                                                                              >  1:4914881/1‑140 (MQ=255)

              cacaTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTcaa                                                                                                                        >  1:2042041/1‑140 (MQ=255)

              cacaTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTcaa                                                                                                                        >  2:1213823/1‑140 (MQ=255)

              cacaTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCATCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATCCGGCTATTTcaa                                                                                                                        >  2:3771612/1‑140 (MQ=255)

              cacaTCAAGGTCAAACAACGGTCAATTTTGCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTcaa                                                                                                                        >  1:1754837/1‑140 (MQ=255)

                caTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTcaaca                                                                                                                      >  1:2768292/1‑140 (MQ=255)

                caTCAACGTCACACAACGGTCAATTTTTCAGCGTAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAACTTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGCATATGCGGCTATTGtaaca                                                                                                                      >  2:2199081/1‑140 (MQ=255)

                  tCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACaaa                                                                                                                    >  1:550944/1‑140 (MQ=255)

                   cAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAAt                                                                                                                   >  1:2815612/1‑140 (MQ=255)

                   cAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAAt                                                                                                                   >  1:5309032/1‑140 (MQ=255)

                   cAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAAt                                                                                                                   >  2:2566657/1‑140 (MQ=255)

                   cAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAAt                                                                                                                   >  1:3239276/1‑140 (MQ=255)

                      ggTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCt                                                                                                                >  1:3490997/1‑140 (MQ=255)

                         cAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGt                                                                                                             <  2:4885959/140‑1 (MQ=255)

                          aaaCAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGtt                                                                                                            >  1:4763632/1‑140 (MQ=255)

                           aacaacGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGttt                                                                                                           >  1:3010244/1‑140 (MQ=255)

                           aacaacGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGgtt                                                                                                           >  1:860824/1‑140 (MQ=255)

                                cGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGa                                                                                                      <  2:2495870/140‑1 (MQ=255)

                                 ggTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGTAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                     >  1:329385/1‑140 (MQ=255)

                                 ggTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                     <  2:4450569/140‑1 (MQ=255)

                                 ggTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                     <  2:3432416/140‑1 (MQ=255)

                                 ggTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                     >  1:2858463/1‑140 (MQ=255)

                                  gTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGTGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAAGGCTGTTTTTTTGAAt                                                                                                    >  1:1113679/1‑140 (MQ=255)

                                  gTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAAt                                                                                                    >  1:4565390/1‑140 (MQ=255)

                                  gTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAAt                                                                                                    >  2:4028585/1‑140 (MQ=255)

                                  gTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAAt                                                                                                    >  1:166561/1‑140 (MQ=255)

                                      aTTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCAt                                                                                                <  1:4653239/140‑1 (MQ=255)

                                        ttttCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATaa                                                                                              <  2:1611579/140‑1 (MQ=255)

                                        ttttCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATaa                                                                                              <  2:2290636/140‑1 (MQ=255)

                                               cgcAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACaaa                                                                                       <  2:4563995/140‑1 (MQ=255)

                                                gcAAGGCTGGCGGATTCGCAGCCTCAGGGAGCCAATTTTACCTCGTTGGTATCATCAGTCGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTGTGAATGCATAACGACAAAg                                                                                      <  1:2944084/140‑1 (MQ=255)

                                                gcAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAg                                                                                      <  1:3766288/140‑1 (MQ=255)

                                                    ggCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGAtct                                                                                  <  2:3671094/140‑1 (MQ=255)

                                                    ggCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGAtct                                                                                  <  2:2352158/140‑1 (MQ=255)

                                                              ttAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTgg                                                                        >  2:321040/1‑140 (MQ=255)

                                                                agcagcCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCa                                                                      <  1:99963/140‑1 (MQ=255)

                                                                agcagcCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCa                                                                      >  2:4249766/1‑140 (MQ=255)

                                                                agcagcCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCa                                                                      >  1:2004418/1‑140 (MQ=255)

                                                                   agcCTCAGGGAGCAAATTTTACCTCCTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGAc                                                                   <  2:2023501/140‑1 (MQ=255)

                                                                       tctgggAGCAAATCTTACGTTGTTGGTATCATCAGTGGTTGAGCGTGCTAGGGGTGAGCGTATAGAAGATGCGGCTATCTCAACAAGTGCGGGTTTTTTGAATGCAGAACGCCGAAGAGCTCTCGACGTGCCAGACaagc                                                               >  2:2738278/4‑137 (MQ=255)

                                                                       tCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTc                                                               >  2:835293/1‑140 (MQ=255)

                                                                             aGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGa                                                         <  1:5108881/140‑1 (MQ=255)

                                                                             aGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTCGATAACGGGTAGAATATGCGGCTATTTCAACAAGTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGa                                                         <  2:1493785/140‑1 (MQ=255)

                                                                             aGCAAATTTTACCTCGGTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGa                                                         <  2:726173/140‑1 (MQ=255)

                                                                              gCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGAc                                                        <  1:2083725/140‑1 (MQ=255)

                                                                               cAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACt                                                       <  1:4374959/140‑1 (MQ=255)

                                                                               cAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACt                                                       >  1:1290161/1‑140 (MQ=255)

                                                                               cAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACt                                                       <  2:3703336/140‑1 (MQ=255)

                                                                                     ttACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGcc                                                 <  2:5314508/140‑1 (MQ=255)

                                                                                     ttACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGcc                                                 <  2:2116872/140‑1 (MQ=255)

                                                                                     ttACCTCGTTGGTATCATCAGTCGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACAGTCCGCCGACTGTGGcc                                                 <  2:5218158/140‑1 (MQ=255)

                                                                                            gTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCAtt                                          >  1:3242482/1‑140 (MQ=255)

                                                                                             ttGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTg                                         >  1:3074997/1‑140 (MQ=255)

                                                                                             ttGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAAAGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTg                                         >  1:4261605/1‑140 (MQ=255)

                                                                                              tGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTgc                                        <  1:150279/140‑1 (MQ=255)

                                                                                               ggTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTgcg                                       <  2:2995486/140‑1 (MQ=255)

                                                                                               ggTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTgcg                                       <  2:4679705/140‑1 (MQ=255)

                                                                                                      tcaGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCa                                >  2:3016271/1‑140 (MQ=255)

                                                                                                        aGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCaaa                              <  2:4961248/140‑1 (MQ=255)

                                                                                                         gTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACTTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                             <  2:4652242/140‑1 (MQ=255)

                                                                                                         gTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                             <  1:3133016/140‑1 (MQ=255)

                                                                                                         gTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                             <  1:598474/140‑1 (MQ=255)

                                                                                                         gTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                             <  2:4343433/140‑1 (MQ=255)

                                                                                                         gTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                             <  1:2552185/140‑1 (MQ=255)

                                                                                                         gTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGACAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                             <  2:4898337/140‑1 (MQ=255)

                                                                                                          tGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGc                            <  1:3959605/140‑1 (MQ=255)

                                                                                                          tGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGc                            >  2:165281/1‑140 (MQ=255)

                                                                                                          tGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGc                            >  1:4017615/1‑140 (MQ=255)

                                                                                                              ttGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGt                        <  2:513766/140‑1 (MQ=255)

                                                                                                               tGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTc                       >  1:1778091/1‑140 (MQ=255)

                                                                                                               tGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTc                       >  2:3348676/1‑140 (MQ=255)

                                                                                                               tGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGGTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCACCGACTGTGGCCAACCATTTCGCCTTTCAAAGCGGTTc                       >  2:646175/1‑140 (MQ=255)

                                                                                                               tGGCGTGCCAGTGGATAACAGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTc                       <  2:2015451/140‑1 (MQ=255)

                                                                                                                   gTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGAt                   <  2:1102375/140‑1 (MQ=255)

                                                                                                                      ccAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGt                >  1:3614116/1‑140 (MQ=255)

                                                                                                                      ccAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGt                >  2:5057222/1‑140 (MQ=255)

                                                                                                                      ccAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGt                >  2:2814776/1‑140 (MQ=255)

                                                                                                                        aGTGGATATCGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTgg              <  2:3908099/140‑1 (MQ=255)

                                                                                                                        aGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTgg              <  1:4122784/140‑1 (MQ=255)

                                                                                                                        aGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTgg              <  2:1371007/140‑1 (MQ=255)

                                                                                                                         gTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggc             <  1:1426754/140‑1 (MQ=255)

                                                                                                                         gTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggc             <  1:3908442/140‑1 (MQ=255)

                                                                                                                         gTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggc             <  1:80708/140‑1 (MQ=255)

                                                                                                                               aaCGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGt       <  2:3940395/140‑1 (MQ=255)

                                                                                                                               aaCGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGt       <  2:191398/140‑1 (MQ=255)

                                                                                                                                aCGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcgggc         >  1:237340/1‑137 (MQ=255)

                                                                                                                                 cGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAg     >  1:1335580/1‑140 (MQ=255)

                                                                                                                                 cGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAg     >  1:653255/1‑140 (MQ=255)

                                                                                                                                 cGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGGCTGATCGTGGCGGGCGTAg     >  2:1285629/1‑140 (MQ=255)

                                                                                                                                    gTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATTTCTCTACGTGGCAGACATTCTGCCGACTGTGGCTAACCATTGAGCCTTTCAAAGTGTGACTGATCCTGGCGGGCGTacagt  >  2:2696848/1‑136 (MQ=255)

                                                                                                                                    gTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGt  >  1:4516455/1‑140 (MQ=255)

ATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGT  >  NZ_CP025268/973555‑973826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`