Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 112,069 G→A A121V (GCG→GTG)  zapD ← cell division protein ZapD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268112,0690GA99.7% 1099.9 / NA 297A121V (GCG→GTG) zapDcell division protein ZapD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (176/120);  minor base T (1/0);  total (177/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

CCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGT  >  NZ_CP025268/111932‑112205
                                                                                                                                         |                                                                                                                                        
ccTGGGCGAGCGGGTTCAGGCTGGCACTCCAGGTTTATACCTGGCTGTCGCGCTGCGCCGGGGGTAGATGCAGCCAAATGTGCATTGTAGGAAACTCAAAGCTGCAACAGCCGCCTGGGATCCTCAGTCGCTGACGCAcc                                                                                                                                        >  2:2667973/1‑140 (MQ=255)
 cTGGGTGAGCGGGTTCAGGCTGGCCATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCa                                                                                                                                       >  1:2110483/1‑140 (MQ=255)
 cTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCa                                                                                                                                       >  1:3965320/1‑140 (MQ=255)
  tGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCag                                                                                                                                      >  2:3239891/1‑140 (MQ=255)
  tGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCag                                                                                                                                      >  1:2473081/1‑140 (MQ=255)
  tGGGTGAGCGGCTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCGATGTAGGTAAAACAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCag                                                                                                                                      >  1:4431509/1‑140 (MQ=255)
   gggTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCaga                                                                                                                                     >  2:2741829/1‑140 (MQ=255)
    ggTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCagag                                                                                                                                    <  2:4668829/140‑1 (MQ=255)
        aGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAAt                                                                                                                                <  1:3776069/140‑1 (MQ=255)
        aGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAAt                                                                                                                                >  2:3417019/1‑140 (MQ=255)
        aGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAAt                                                                                                                                >  2:3576193/1‑140 (MQ=255)
        aGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAAt                                                                                                                                >  2:428247/1‑140 (MQ=255)
             gTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAAc                                                                                                                           <  2:762801/140‑1 (MQ=255)
              ttCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACg                                                                                                                          >  1:2093548/1‑140 (MQ=255)
              ttCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACg                                                                                                                          >  1:4901667/1‑140 (MQ=255)
                   gCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCtt                                                                                                                     >  1:1262040/1‑140 (MQ=255)
                       gCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACg                                                                                                                 >  1:2224133/1‑140 (MQ=255)
                       gCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACg                                                                                                                 >  1:176641/1‑140 (MQ=255)
                       gCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATAAAAGCTGCAACAGCCGCCGGGGATGCTCATTCGCTGACGAACCAGAGCAATCAAACGATCTTCACg                                                                                                                 >  1:1397143/1‑140 (MQ=255)
                       gCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGGAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATGAAACTATCTTCACg                                                                                                                 >  2:1096897/1‑140 (MQ=255)
                       gCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGCAGATGCAGCCAAATGGGCGCTGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACAAGAGCAATCAAACGATCTTCACg                                                                                                                 >  2:1237431/1‑140 (MQ=255)
                       gCAATCCAGGTTTCTACCGGGCTGTCGCGCGGCGCCTGGGGTAGATGCAGCCGGATGTGCAATGTAGGTAAATCAAGGCTGCAACAGCCGCCTGGGATGCGCTGTCGCTGACGCACCAGAGCAATCGAAGGATCTTCACg                                                                                                                 >  2:3758217/1‑140 (MQ=255)
                        ccaTCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGc                                                                                                                <  2:2905955/138‑1 (MQ=255)
                             cAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGaaa                                                                                                           <  2:873639/140‑1 (MQ=255)
                                         tGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcg                                                                                               >  2:4922587/1‑140 (MQ=255)
                                          ggCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGGAAATCAAAGCTGCAACAGCCGCCTGGGATGCGCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgc                                                                                              >  2:4059862/1‑140 (MQ=255)
                                           gCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcg                                                                                             <  1:156291/140‑1 (MQ=255)
                                                cgcgCTGCGCATGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGCTAcgcggcgcg                                                                                        <  2:458257/140‑1 (MQ=255)
                                                  cgcTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGa                                                                                      <  1:2257932/140‑1 (MQ=255)
                                                  cgcTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAAGCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGa                                                                                      <  2:5082714/140‑1 (MQ=255)
                                                    cTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGaaa                                                                                    <  1:588057/140‑1 (MQ=255)
                                                    cTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACACACCAGAGCAATAAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGaaa                                                                                    <  2:3278717/140‑1 (MQ=255)
                                                       cgcCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTa                                                                                 >  1:3413102/1‑140 (MQ=255)
                                                                 aGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTccc                                                                       >  1:4233673/1‑140 (MQ=255)
                                                                   aagCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccg                                                                     <  2:4467351/138‑1 (MQ=255)
                                                                   aTGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccg                                                                     <  1:474343/140‑1 (MQ=255)
                                                                   aTGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccg                                                                     >  1:2028924/1‑140 (MQ=255)
                                                                   aTGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccg                                                                     <  1:1048993/140‑1 (MQ=255)
                                                                   aTGCAGCCAAAAGTGCAATGTAGGTAAATCAAAGCTGCAACAGACGCCTGGGATGCTCAGTCGCTGCCCCACCAGCGCAATCAAACGATCTCCACCCAGAAATTGCCCGATACGCGCCGCGGAAAGTAATACGCTccccc                                                                     >  2:3635994/1‑139 (MQ=255)
                                                                    tGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgc                                                                    >  1:4944861/1‑140 (MQ=255)
                                                                       aGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGGCTTGGATGCTCAGTCGCTGACGCACAAGAGCAATCAAACGATCTACACGAAGAAGTTGCCCGATACGCGGCGAGAAAATTGAGACGCTCCCcgccgc                                                                 >  2:2055883/1‑140 (MQ=255)
                                                                       aGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCcgccgc                                                                 >  2:4495251/1‑140 (MQ=255)
                                                                       aGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCcgccgc                                                                 >  1:1100054/1‑140 (MQ=255)
                                                                        gCCAAATGTGCAATGTAGGTAAATCAGAGCTGCAACAGCCGCGTGGGATGCTCAGTCGCTGACGCACCAGAGCCATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAGTGAATACGCGCCCCGCCGCt                                                                >  2:4254190/1‑140 (MQ=255)
                                                                        gCCAAATGTGCAATGTAGGTAAATCAAAGTGGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCt                                                                <  2:635548/140‑1 (MQ=255)
                                                                        gCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCt                                                                >  2:2484448/1‑140 (MQ=255)
                                                                        gCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCt                                                                >  2:2073791/1‑140 (MQ=255)
                                                                                tgCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAActg                                                        <  2:5312920/140‑1 (MQ=255)
                                                                                tgCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAActg                                                        >  2:2611055/1‑140 (MQ=255)
                                                                                       tAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAAt                                                                               <  1:2073192/110‑1 (MQ=255)
                                                                                       tAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAAtt                                                 >  2:4032742/1‑140 (MQ=255)
                                                                                       tAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAAtt                                                 <  2:4105418/140‑1 (MQ=255)
                                                                                         ggTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTaa                                               <  1:4120342/140‑1 (MQ=255)
                                                                                         ggTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTaa                                               <  2:1534105/140‑1 (MQ=255)
                                                                                         ggTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTaa                                               <  2:2461512/140‑1 (MQ=255)
                                                                                            aaaTCAAAGCTGCAACAGCCGCCTGCGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGc                                            <  2:3529996/140‑1 (MQ=255)
                                                                                             aaTCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACg                                                                                                                 >  1:46352/1‑70 (MQ=255)
                                                                                             aaTCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTAAACTGCTGAATTAATGCt                                           >  2:3802346/1‑140 (MQ=255)
                                                                                              acccaaGCTGCAACCGGAGCCCGAGCTGCTCAGTCGCTGAAGCACCAGCGCAATCACACGGCCCTAACGCAGAAATCGCCCGATACGCGGCGCGGAAAATAATACGCCCCCCGCCGCTTTTAACTGCCGAATTAATGCtt                                          <  2:3028978/136‑1 (MQ=255)
                                                                                              aTCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCtt                                          <  1:3706730/140‑1 (MQ=255)
                                                                                               tCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAAACAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTGTTAACTGCTGAATTAATGCtgc                                         >  2:3364395/1‑138 (MQ=255)
                                                                                                   aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                                     <  2:1932654/140‑1 (MQ=255)
                                                                                                   aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                                     >  1:185157/1‑140 (MQ=255)
                                                                                                   aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                                     <  1:430095/140‑1 (MQ=255)
                                                                                                   aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                                     <  2:149639/140‑1 (MQ=255)
                                                                                                   aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                                     <  2:3942466/140‑1 (MQ=255)
                                                                                                   aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                                     <  1:387222/140‑1 (MQ=255)
                                                                                                   aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                                     >  1:5169190/1‑140 (MQ=255)
                                                                                                   aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                                     <  1:660318/140‑1 (MQ=255)
                                                                                                    gCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc                                    >  2:1380642/1‑140 (MQ=255)
                                                                                                    gCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACACGAGCAATCAAACGATCTTCACGCAGAAATTACCCGATACGCGGCGGGGAACTTAATACGCTAACCGCCGCTTTTAACTGCTGAATTAATGCGTCAGTAc                                    >  2:3689108/1‑140 (MQ=255)
                                                                                                        cAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGct                                >  2:4389445/1‑140 (MQ=255)
                                                                                                        cAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGct                                <  1:3350329/140‑1 (MQ=255)
                                                                                                             gccgccTGGTATGCCCAGCCGCTGACGCACCAGCGCAATCAAACGATCTTCACGCACAAATTGCCCACTACGCGGCGCGGAAATTACTACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCTATCCGGCTCTGGt                           <  2:5194583/140‑1 (MQ=255)
                                                                                                              ccgccTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTc                          >  1:3103004/1‑140 (MQ=255)
                                                                                                              ccgccTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTc                          <  1:819535/140‑1 (MQ=255)
                                                                                                              ccgccTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTc                          <  1:3239891/140‑1 (MQ=255)
                                                                                                               cgccTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTcc                         >  1:4026162/1‑140 (MQ=255)
                                                                                                                gccTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCa                        >  2:2400567/1‑140 (MQ=255)
                                                                                                                  cTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACg                      <  1:356315/140‑1 (MQ=255)
                                                                                                                   tGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGCTACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCActc                     >  2:5054519/1‑138 (MQ=255)
                                                                                                                       aTGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAgg                 <  1:1897762/140‑1 (MQ=255)
                                                                                                                       aTGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAgg                 <  1:2647365/140‑1 (MQ=255)
                                                                                                                       aTGCTCAGTCGCTGACGCACCAGAGCAATCAAACGAGCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAgg                 <  2:213302/140‑1 (MQ=255)
                                                                                                                        tGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGc                >  1:1356923/1‑140 (MQ=255)
                                                                                                                         gCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCa               >  1:374300/1‑140 (MQ=255)
                                                                                                                          cacAGTCGCGGACGCACCAGAGCAATCAGACGATCTTCACGCAGCAATTGGCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCCTCAATACGGCCCTGGTCCACGCCCGGccc              >  2:2161793/3‑138 (MQ=255)
                                                                                                                          cTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAActg                                                        >  1:1283904/1‑98 (MQ=255)
                                                                                                                          cTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAActg                                                        <  2:1283904/98‑1 (MQ=255)
                                                                                                                          cTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCAc              <  2:5259828/140‑1 (MQ=255)
                                                                                                                          cTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCAc              >  2:104266/1‑140 (MQ=255)
                                                                                                                            cAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACTGCTCTGGTCCACGCCAGGCACGc            >  2:1822625/1‑140 (MQ=255)
                                                                                                                            cAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGc            <  2:4404766/140‑1 (MQ=255)
                                                                                                                            cAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGc            <  1:5330121/140‑1 (MQ=255)
                                                                                                                               tCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCaa         >  2:3171544/1‑140 (MQ=255)
                                                                                                                                cGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAAt        >  1:2135740/1‑140 (MQ=255)
                                                                                                                                 gCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATc       <  1:2850148/140‑1 (MQ=255)
                                                                                                                                 gCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATc       <  1:4059862/140‑1 (MQ=255)
                                                                                                                                 gCTGACGCAACAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTATGGTCCACGCCAGGCACGCCAATc       <  2:4842628/140‑1 (MQ=255)
                                                                                                                                    gACGCACCAGAGCAATCAAACGATCTTGACGCAGACATTGCCCGATACGCGGCGCGGAACTTAATACGCTCCCCGCCGCTTTTCACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAAGCCAg    >  2:3923673/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGT  >  NZ_CP025268/111932‑112205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑