BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,293,179	A→G	noncoding (36/132 nt)	CXP41_RS06840 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,293,179	0	A	G	99.4%	525.8 / ‑3.6	172	noncoding (36/132 nt)	CXP41_RS06840	RtT sRNA
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (109/62); minor base C (1/0); total (110/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 172 total aligned reads displayed.

AGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAC  >  NZ_CP025268/1293060‑1293311

    tagtgatttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                              <  2:3762755/133‑1 (MQ=11)

         aattttGAGAGTGATGGTGGTGGGGGAAGGCTTACTCAGCGCTGCGCGCTTCGCCATTCGGGTCGTGGCCTGCGGCAACGATCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGCAGCTTCTCATCCTTCCCCGCATgtg                                                                                                         <  2:3320818/138‑3 (MQ=11)

          tttttGGAAGTGATGGTGGTGGGGGAAGGATTACTCCGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGc                                                                                                        <  2:5114123/139‑1 (MQ=11)

           ttttGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                       <  1:809610/140‑1 (MQ=255)

           tcttcagaGTGATGGTGGTGGGGAAAGGTTGACTGCGCGCGGCGCGCTTCGGCGTCAGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                       <  2:557184/133‑1 (MQ=11)

                                         tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGAGAGt                                                                         >  1:4596190/1‑140 (MQ=11)

                                           cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGCTTGGGAGTTc                                                                       >  2:4403940/1‑140 (MQ=11)

                                             cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                                     <  1:4555213/140‑1 (MQ=19)

                                             cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCACGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTGCAg                                                                     <  1:4555863/140‑1 (MQ=255)

                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                                   <  1:2752693/140‑1 (MQ=255)

                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                                  >  2:3072183/1‑140 (MQ=255)

                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                                  >  2:5222113/1‑140 (MQ=255)

                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                                  >  1:2300706/1‑140 (MQ=255)

                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                                  >  2:5285833/1‑140 (MQ=255)

                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGATAGTTCAgggg                                                                  >  1:423106/1‑139 (MQ=11)

                                                   tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGTTGACGCTCGAGCCGAACCTTACTCGAAGCTACTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGCTTGACAGTTCAGGGACtt                                                               >  2:4717443/1‑140 (MQ=11)

                                                   tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGACAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtt                                                               >  2:2868057/1‑140 (MQ=255)

                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTTAGAGTTCAGGGACtttt                                                             >  1:2543438/1‑140 (MQ=255)

                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGCGTTCAGGGACtttt                                                             >  1:3655198/1‑140 (MQ=255)

                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                             >  1:5105565/1‑140 (MQ=255)

                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                             >  2:4122531/1‑140 (MQ=255)

                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                             >  1:3582965/1‑140 (MQ=255)

                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                             >  1:4390937/1‑140 (MQ=255)

                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTAAGGGACtttt                                                             >  1:1426316/1‑140 (MQ=255)

                                                      gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTg                                                            <  1:1808345/140‑1 (MQ=255)

                                                        gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                                          <  1:4247545/140‑1 (MQ=255)

                                                        gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                                          <  1:1311048/140‑1 (MQ=255)

                                                         cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                         <  1:1461873/140‑1 (MQ=255)

                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCTCTCGCAGACGCTCGCCCTGCGCCTCAGTCGACGCTTCTCATCCTTTCCCGCCGGGGCAGTATATTTGTTTGCGGATTCGGTTGAGAGTTCAGGGACTTTTGAAAg                                                        >  2:2898291/1‑140 (MQ=11)

                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCGCGCATGGGCAGAATATTTGATTGCGGATTCGTTGGAGAGTTCAGGGACTTTTGAAAg                                                        >  2:4015429/1‑140 (MQ=11)

                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                                        >  1:2692134/1‑140 (MQ=255)

                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                                        >  1:2691281/1‑140 (MQ=255)

                                                            cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTg                                                      >  1:388317‑M2/1‑139 (MQ=255)

                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTTGAGAGTTCAGGGACTTTTGAAAGTga                                                     <  2:4293716‑M2/140‑3 (MQ=255)

                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                                     >  1:965747‑M2/1‑138 (MQ=255)

                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTGCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                                     >  1:4606061‑M2/1‑138 (MQ=255)

                                                               ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                                   >  1:2999949‑M2/1‑136 (MQ=255)

                                                               ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                                   >  1:2995036‑M2/1‑136 (MQ=255)

                                                               ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                                   <  2:4217107‑M2/140‑5 (MQ=255)

                                                                  tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtg                                                <  2:545319‑M2/140‑8 (MQ=255)

                                                                    gggTCGTTGCCTGCGGCCACGCTCTCTCGCTGTCGCTAGGGCCGACCCCTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                              >  2:1148298‑M2/1‑131 (MQ=255)

                                                                    gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                              <  1:4230‑M2/140‑10 (MQ=255)

                                                                    gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                              >  2:3888672‑M2/1‑131 (MQ=255)

                                                                    gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                              >  1:3358083‑M2/1‑131 (MQ=255)

                                                                    gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                              >  2:4895769‑M2/1‑131 (MQ=255)

                                                                    gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                              >  2:324718‑M2/1‑131 (MQ=255)

                                                                    gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                              >  2:1961948‑M2/1‑131 (MQ=255)

                                                                       tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggg                                           >  1:5227377‑M2/1‑128 (MQ=255)

                                                                       tCGCTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGTGACTTTTGAAAGTgatggtggtggg                                           >  2:2012982‑M2/1‑128 (MQ=255)

                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                                         >  2:1435919‑M2/1‑126 (MQ=255)

                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                                         >  2:830190‑M2/1‑126 (MQ=255)

                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                                         >  1:1033309‑M2/1‑126 (MQ=255)

                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                                         >  2:2348093‑M2/1‑126 (MQ=255)

                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                                         >  1:1050988‑M2/1‑126 (MQ=255)

                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                                         >  1:2222352‑M2/1‑126 (MQ=255)

                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                                         >  1:4016917‑M2/1‑126 (MQ=255)

                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                                         >  1:4745005‑M2/1‑126 (MQ=255)

                                                                           tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaa                                       <  2:3680191‑M2/140‑17 (MQ=255)

                                                                              cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGAATATTTGAATGCGGTTTCGCGTGGGGGTACAGGTGTTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                    <  2:5048250/140‑1 (MQ=11)

                                                                              cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTgatggtggtgggggaagga                                    >  2:1109869‑M2/1‑121 (MQ=255)

                                                                              cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                    >  1:3978637‑M2/1‑121 (MQ=255)

                                                                              cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                    >  1:4184561‑M2/1‑121 (MQ=255)

                                                                              cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                    >  2:3584322‑M2/1‑121 (MQ=255)

                                                                              cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                    >  1:1335518‑M2/1‑121 (MQ=255)

                                                                              cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                    >  1:4509990‑M2/1‑121 (MQ=255)

                                                                              cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                    >  1:4855633‑M2/1‑121 (MQ=255)

                                                                              cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                    >  1:545319‑M2/1‑121 (MQ=255)

                                                                              cTGAGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                    >  2:506057‑M2/1‑121 (MQ=255)

                                                                                gCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggatt                                  <  1:3660301‑M2/140‑22 (MQ=255)

                                                                                   gCAACGCTCTCTCGCTTGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattact                               >  1:1278131‑M2/1‑116 (MQ=255)

                                                                                   gCAACGCTCTCTCGCTGGCGCTCAAGTCGACCCTTAGGAGCAGCTTCTCATCCTGACCCGCATGGGAAGAATCGTTGATTGCGGATTCGGTGGAGAATTGAGGGACTTTTGAAAGCGATGGTGGTGGGGGAAGGAttact                               <  2:3387365/140‑4 (MQ=0)

                                                                                   gCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattact                               >  1:4349306‑M2/1‑116 (MQ=255)

                                                                                   gCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattact                               >  2:3387916‑M2/1‑116 (MQ=255)

                                                                                   gCAACGCTCTCTCGCTGACGATCGAGCCGAAGCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattact                               >  2:2124876‑M2/1‑116 (MQ=255)

                                                                                      aCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcag                            >  1:1187389‑M2/1‑113 (MQ=255)

                                                                                       cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagc                           <  1:2045618‑M2/140‑29 (MQ=255)

                                                                                         ctctctCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgc                         <  2:3013390‑M2/140‑31 (MQ=255)

                                                                                         ctctctCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgc                         <  1:652243‑M2/140‑31 (MQ=255)

                                                                                          ttgcgcGCTGGCGCCAGAGTCGAACCTTGGTCGAAGCTTCTCCTCCTTCCCCGCATGGGCAGAAGATTTAATTGGGGATTCGTAGGAGGGTTTAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCg                        <  2:1802806/135‑1 (MQ=11)

                                                                                           ctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTgatggtggtgggggaaggattactcagcgctg                       >  2:1625520‑M2/1‑108 (MQ=255)

                                                                                           ctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTCCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctg                       <  2:2165356‑M2/140‑33 (MQ=255)

                                                                                            tctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggcttactcagcgctgc                      <  1:861359‑M2/140‑34 (MQ=255)

                                                                                            tctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgc                      >  1:157688‑M2/1‑107 (MQ=255)

                                                                                            cctcgCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATACCTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGCAGGTgaaggtggtgggggaaggattactcagcgctgc                      >  2:3412490‑M2/2‑107 (MQ=255)

                                                                                              tcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgc                    <  1:4435688‑M2/140‑36 (MQ=255)

                                                                                               cGCTGGCGCTTGAGCCGAACCTTAGTCGTAGCTTCTCCTCCTTACCCGCGTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcg                   >  2:4365778‑M2/1‑104 (MQ=255)

                                                                                               cGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGGgatggtggtgggggaaggattactcagcgctgcgcg                   >  2:2430848‑M2/1‑104 (MQ=255)

                                                                                                 cTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgct                 <  1:5239390‑M2/140‑39 (MQ=255)

                                                                                                  tgacgctcgAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGACAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgctt                >  1:2868057‑M2/4‑101 (MQ=255)

                                                                                                   gacgctcGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttc               >  1:2338333‑M2/3‑100 (MQ=255)

                                                                                                     cgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAGTTCAGGGGCTTTTGAAAGTgatggtggtgggggaaggattagtcagcgctgcgcggttcgc             <  2:3512032‑M2/140‑43 (MQ=255)

                                                                                                      gcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcgcc            <  2:4526730‑M2/140‑44 (MQ=255)

                                                                                                       cTCGAGTCGAACCGTGGTCGAAGCTTCTCAGCCTCCACCGCATGGGAAGGATATTTAAGTGCGGATTCGTTGGGGAGGTCGGGGACGTGTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg           <  2:4596222/140‑1 (MQ=9)

                                                                                                        tCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcgccct          <  2:3945700‑M2/140‑46 (MQ=255)

                                                                                                         cggctcGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGAATATTTAATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGGCAGTGATGGTGGTGGGGGAAGGGTTCGAACCTTCGAAGTCGATGACGGCa         <  2:2765796/135‑1 (MQ=11)

                                                                                                           aGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                                   <  2:4211120/80‑1 (MQ=255)

                                                                                                           aGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                                   >  1:4211120/1‑80 (MQ=255)

                                                                                                            gCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcgcccttcgg      <  1:1438550‑M2/140‑50 (MQ=255)

                                                                                                                aaCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgggcgcttcgcccttcgggtcg  <  2:4280829‑M2/140‑54 (MQ=255)

                                                                                                                aaCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcgcccttcgggtcg  >  1:2002130‑M2/1‑87 (MQ=255)

AGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAC  >  NZ_CP025268/1293060‑1293311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`