BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,394,060	T→C	pseudogene (313/966 nt)	ycjG →	L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,394,060	0	T	C	99.7%	1199.1 / NA	362	pseudogene (313/966 nt)	ycjG	L‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (179/181); total (180/182)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 364 total aligned reads displayed.

GGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACTGAAAGT  >  NZ_CP025268/1393924‑1394196

ggCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACCCGGGAGGAGGTGC‑AAAAAGTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGGACTGTGGGATCTGGCCGCGCGAAGACAGCAGCaac                                                                                                                                       >  2:2210501/1‑139 (MQ=255)

      aaTTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCgctgg                                                                                                                                 >  2:135772/1‑140 (MQ=255)

      aaTTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCgctgg                                                                                                                                 <  1:3235051/140‑1 (MQ=255)

      aaTTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCgctgg                                                                                                                                 <  1:2801628/140‑1 (MQ=255)

            gAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGAtt                                                                                                                           >  1:2752336/1‑140 (MQ=255)

             aGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGTGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCACTCGCTGGCTGAttt                                                                                                                          >  1:422694/1‑140 (MQ=255)

                gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                       <  2:4998823/140‑1 (MQ=255)

                gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                       <  1:1923329/140‑1 (MQ=255)

                gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                       <  2:460197/140‑1 (MQ=255)

                gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                       <  1:532931/140‑1 (MQ=255)

                gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                       <  1:691282/140‑1 (MQ=255)

                 tcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                       <  2:1727212/140‑1 (MQ=255)

                 tcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATc                                                                                                                      >  1:2663566/1‑140 (MQ=255)

                 tcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATc                                                                                                                      >  1:286569/1‑140 (MQ=255)

                  cgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGTGCTGC‑AACAAATTCTCCCTGCCTGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGTGATCTGGCCGCGCGAATACAGCAGCAATCGCTGGCTGATTTGATCg                                                                                                                     >  2:4878278/1‑140 (MQ=255)

                     gCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCa                                                                                                                  <  1:2612186/140‑1 (MQ=255)

                          aaCTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACg                                                                                                             <  2:5353739/140‑1 (MQ=255)

                          aaCTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTCGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCGGATTTGATCGGCATAACg                                                                                                             <  1:875986/140‑1 (MQ=255)

                             tAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGGACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCtt                                                                                                          <  1:4782941/140‑1 (MQ=255)

                                 gacaGGGGTGGCACGGGAGGCGGTGC‑AAAGAAGCATCCCTGCCGGGGCGGCACGTGGTGCGATGGATTGTGCATTGTGGGATCTGGCCGCGGGTAGACAGCAGCAATCGCTGGCGGATGTGATCGGCATAACGCTTcgcg                                                                                                      <  2:2570213/137‑1 (MQ=255)

                                   aaGGGCTGACACGGGAGGATTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGTATAACGCTTCCCgag                                                                                                    <  2:1184133/140‑1 (MQ=255)

                                   aaGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                                    <  1:1782529/140‑1 (MQ=255)

                                   aaGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                                    <  2:857116/140‑1 (MQ=255)

                                    aGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgaga                                                                                                   >  2:3607919/1‑140 (MQ=255)

                                    aGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgaga                                                                                                   >  1:430685/1‑140 (MQ=255)

                                      ggCTGACACGGGAGGGGTTGC‑AACAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACCGCAGCAATCGCTGGCTGGTTTGATCGGCATAACGCTTCCCGAGACa                                                                                                 >  2:2030909/1‑140 (MQ=255)

                                       gCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAg                                                                                                >  1:5250877/1‑140 (MQ=255)

                                       gCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAg                                                                                                >  1:2887949/1‑140 (MQ=255)

                                       gCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAg                                                                                                >  2:3193170/1‑140 (MQ=255)

                                       gCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCAGAACGCTTCCCGAGACAg                                                                                                >  2:1269744/1‑140 (MQ=255)

                                           acacGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                                            <  1:3734964/140‑1 (MQ=255)

                                             acGGGAGGAGTTGC‑AAAAGATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGTAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCa                                                                                          <  2:3818990/140‑1 (MQ=255)

                                             acGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCa                                                                                          <  2:2360031/140‑1 (MQ=255)

                                             acGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCa                                                                                          <  2:1022312/140‑1 (MQ=255)

                                                ggaggaGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTg                                                                                       >  1:3954308/1‑140 (MQ=255)

                                                ggaggaGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGGTATCACTg                                                                                       >  1:3163818/1‑140 (MQ=255)

                                                 gaggagTTGC‑ACACCATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGc                                                                                      >  1:354995/1‑140 (MQ=255)

                                                 gaggagTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGc                                                                                      >  2:3777102/1‑140 (MQ=255)

                                                 gaggagTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGc                                                                                      >  1:1212026/1‑140 (MQ=255)

                                                   ggagTTGC‑AAAAAATTCTCCCTGCCGGCGCAGTACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCAGCATAACGCTTCCCGAGACAGTTATCACTGcac                                                                                    <  2:1100783/140‑1 (MQ=255)

                                                    gagTTGC‑AAAAAATTCTCCCTGCAGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGcaca                                                                                   <  2:644029/140‑1 (MQ=255)

                                                     agTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAg                                                                                  >  1:298451/1‑140 (MQ=255)

                                                      gTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTACTGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGa                                                                                 <  1:206000/140‑1 (MQ=255)

                                                      gTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGa                                                                                 <  1:2273500/140‑1 (MQ=255)

                                                       ttGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGAGCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGCTCGGCATAACGCTTCCCGAGACAGTGATCACTGCACAGAc                                                                                >  2:1874527/1‑140 (MQ=255)

                                                             aaaaaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTg                                                                           >  1:501359/1‑140 (MQ=255)

                                                             aaaaaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTg                                                                           <  1:845840/140‑1 (MQ=255)

                                                               aaaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTc                                                                         <  2:4797367/140‑1 (MQ=255)

                                                               aaaTACTCCCTGCCGGCGAAGCACGTAATGCGGTGGACTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAGCGGCATAACGCTTCCCGAGACAGTTATCACTGCAAAGACGGTTGTc                                                                         <  2:3163523/140‑1 (MQ=255)

                                                                aaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCa                                                                        >  2:163300/1‑140 (MQ=255)

                                                                    ctcCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                                    <  1:1362192/140‑1 (MQ=255)

                                                                    ctcCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                                    <  2:1575297/140‑1 (MQ=255)

                                                                      cccTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGTGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTa                                                                  <  2:2253573/140‑1 (MQ=255)

                                                                      cccTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGCGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGATTGTCATCGGTa                                                                  <  2:5290280/140‑1 (MQ=255)

                                                                        cTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACg                                                                <  1:3770802/140‑1 (MQ=255)

                                                                         tGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGc                                                               <  1:2455466/140‑1 (MQ=255)

                                                                                 cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGTCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                       <  1:3113800/140‑1 (MQ=255)

                                                                                 cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                       <  2:2874646/140‑1 (MQ=255)

                                                                                 cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                       <  2:2425561/140‑1 (MQ=255)

                                                                                 cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                       <  1:502704/140‑1 (MQ=255)

                                                                                 cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                       <  1:766378/140‑1 (MQ=255)

                                                                                 caacaagTACTGCGCTAAATTCAGCATTGTGGTATCTGGCCGCGCGAAAACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACATTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                       <  2:811219/134‑1 (MQ=255)

                                                                                      cGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGc                                                  <  1:4808904/140‑1 (MQ=255)

                                                                                      cGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGc                                                  <  1:4639972/140‑1 (MQ=255)

                                                                                          aTGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAAt                                              <  1:1112305/140‑1 (MQ=255)

                                                                                            gcgcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAg                                            <  2:2067849/140‑1 (MQ=255)

                                                                                            gcgcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAg                                            <  1:2109903/140‑1 (MQ=255)

                                                                                            gcgcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAg                                            <  2:350894/140‑1 (MQ=255)

                                                                                              gcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTg                                          <  2:3958197/140‑1 (MQ=255)

                                                                                              gcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTg                                          >  2:4150181/1‑140 (MQ=255)

                                                                                                tGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCa                                        >  2:4447550/1‑140 (MQ=255)

                                                                                                 ggATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGAGAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGCTCAGATGGCCAATAGTGCAt                                       >  2:4946886/1‑140 (MQ=255)

                                                                                                  gATGGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATc                                      >  2:4330148/1‑140 (MQ=255)

                                                                                                       tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAacac                                 >  1:4548040/1‑140 (MQ=255)

                                                                                                       tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAacac                                 >  1:71104/1‑140 (MQ=255)

                                                                                                            tgttGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGAGGGCCCATACTGCATCACCACTCTgg                            >  2:3382331/1‑140 (MQ=255)

                                                                                                            tgtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTgg                            >  2:2979980/1‑140 (MQ=255)

                                                                                                            tgtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTgg                            >  2:5058783/1‑140 (MQ=255)

                                                                                                            tgtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTgg                            >  1:189067/1‑140 (MQ=255)

                                                                                                            ggtgGGATCTGGCCGCGCGAAGGCAGCAGCCATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACACACGGGTGTGATGAGTAGGCCTGGTCAGAGGGCCAAAAGTGCATCTACACTCTgg                            >  2:484931/2‑140 (MQ=255)

                                                                                                            cctgcgATCTGGACGCCCGAAGCAAGCAACAATCGCTGGCTGATTTGACCGGCATAACGCTTCCTGATACAGTTATCACAGTAGAGGCGGACGGCATAGGTAAGACTGAGCAGACGGCGAATAGTGCATTAACACTCAgg                            >  2:2676137/6‑140 (MQ=255)

                                                                                                              tgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTCTCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggca                          >  2:3280316/1‑140 (MQ=255)

                                                                                                              tgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggca                          >  1:3666684/1‑140 (MQ=255)

                                                                                                                ggATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggcagg                        <  2:90989/140‑1 (MQ=255)

                                                                                                                 gATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggcaggc                       >  2:4045401/1‑140 (MQ=255)

                                                                                                                 gATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggcaggc                       <  1:4522062/140‑1 (MQ=255)

                                                                                                                 gATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggcaggc                       >  2:5224457/1‑140 (MQ=255)

                                                                                                                 gATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggcaggc                       >  1:3456939/1‑140 (MQ=255)

                                                                                                                 gATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggcaggc                       >  1:914958/1‑140 (MQ=255)

                                                                                                                   tCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGt                                                                                               >  2:1826508/1‑66 (MQ=255)

                                                                                                                     tGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGTCCAATAGTGCATCAACACTCTGGCaggcaggc                   >  2:3718330/1‑140 (MQ=255)

                                                                                                                     tGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCaggcaggc                   >  1:1722954/1‑140 (MQ=255)

                                                                                                                      ggCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCTGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGgcg                  >  1:491346/1‑140 (MQ=255)

                                                                                                                      ggCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGgcg                  >  1:4332364/1‑140 (MQ=255)

                                                                                                                        ccGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGcgcg                >  2:4391699/1‑140 (MQ=255)

                                                                                                                        ccGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAAATTGCCAATAGTTCAGCAACACTCTGGGAAGCAGGcgcg                >  2:2766886/1‑140 (MQ=255)

                                                                                                                          gcgcgAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGaa              >  1:44175/1‑140 (MQ=255)

                                                                                                                          gcgcgAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGaa              <  1:3853148/140‑1 (MQ=255)

                                                                                                                          gcgcgAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGaa              >  1:5183271/1‑140 (MQ=255)

                                                                                                                           cgcgAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGaaa             >  1:2549965/1‑140 (MQ=255)

GGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGC‑AAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACTGAAAGT  >  NZ_CP025268/1393924‑1394196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`