BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	138,217	T→C	F435S (TTT→TCT)	cueO →	multicopper oxidase CueO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	138,217	0	T	C	99.7%	976.8 / NA	293	F435S (TTT→TCT)	cueO	multicopper oxidase CueO
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (149/142); total (151/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

CACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTAATGTCAG  >  NZ_CP025268/138081‑138352

cACGGCACTATGAATCATCTGAACCACGCCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCCGGCGTTTGATATGAACGAGCCGATGTTTGCGGCTGCGAAAGGGCGATACGAACGTTGGGTTATCTCTgg                                                                                                                                      >  2:4668151/1‑140 (MQ=255)

cACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTgg                                                                                                                                      >  2:2367640/1‑140 (MQ=255)

cACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTgg                                                                                                                                      >  1:1555277/1‑140 (MQ=255)

cACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTgg                                                                                                                                      >  1:2389133/1‑140 (MQ=255)

cACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCAACATGGCAACAAAATAAACGGTCAGGCGTTTGATATTAACAAGCCGAATTTTGCGGCGGCGAAAGGGCAATACGAACCTTGGGTTATCTCTgg                                                                                                                                      >  2:117543/1‑140 (MQ=255)

   ggCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCACCAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGt                                                                                                                                   >  1:2670370/1‑140 (MQ=255)

   ggCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTTGCgg                                                                                                                                   >  1:2785574/1‑139 (MQ=255)

   ggCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGt                                                                                                                                   <  1:2842507/140‑1 (MQ=255)

   ggCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGt                                                                                                                                   >  2:5236448/1‑140 (MQ=255)

   ggCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGt                                                                                                                                   >  1:1780492/1‑140 (MQ=255)

   ggCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGt                                                                                                                                   >  1:75890/1‑140 (MQ=255)

   ggCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGATATCTCTGGCgg                                                                                                                                   >  2:3763530/1‑139 (MQ=255)

   ggCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAAAACGAACGTTGGGTTATCTGTGGCGt                                                                                                                                   >  2:1268979/1‑140 (MQ=255)

    gCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTg                                                                                                                                  <  1:5290943/140‑1 (MQ=255)

      aaTATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTggg                                                                                                                                <  1:3425546/140‑1 (MQ=255)

      aaTATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTggg                                                                                                                                <  1:2906977/140‑1 (MQ=255)

        tatGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCg                                                                                                                              >  1:255213/1‑140 (MQ=255)

        tatGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCg                                                                                                                              >  1:3048775/1‑140 (MQ=255)

            aaTCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACat                                                                                                                          <  1:646372/140‑1 (MQ=255)

            aaTCAGATGAACCACGGCGGGAAGTTGGATGTCCACCCTGCCAACAAAGTCAACGGTCAGGCGTTTGATATGAGCAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTTGCCGACat                                                                                                                          <  2:5328946/140‑1 (MQ=255)

             aTCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACatg                                                                                                                         >  1:2132041/1‑140 (MQ=255)

             aTCATATGAACCACGGCCGGAAGGTGGATTTCCACCATGCCAACAAGAGCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGTAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACatg                                                                                                                         <  2:2328490/140‑1 (MQ=255)

              tCATATGAACAACGGCGGGAAGTTCGATTTCCACCATTCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACatga                                                                                                                        <  1:1432807/140‑1 (MQ=255)

                 tatGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGAtgc                                                                                                                     >  1:4293631/1‑140 (MQ=255)

                  atGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGAtgct                                                                                                                    >  2:701322/1‑140 (MQ=255)

                   tGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGAtgctg                                                                                                                   <  1:2033212/140‑1 (MQ=255)

                      aCCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCAt                                                                                                                <  1:132963/140‑1 (MQ=255)

                        cACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATcc                                                                                                              <  1:3456616/140‑1 (MQ=255)

                         aCGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGGGTTTGATATGAACAAGCCGATGTTTGCGGCGGCTAAAGGGCAATACGAACGATAGGTTATCTCTGGCGGGGGAGAAAAGATGCTGCATcgg                                                                                                             >  2:184570/1‑138 (MQ=255)

                          cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGt                                                                                                            >  2:2490199/1‑140 (MQ=255)

                          cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGt                                                                                                            >  2:2522499/1‑140 (MQ=255)

                          cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTGATCTCTGGCGTGGGCGACATGATGCTGCATCCGt                                                                                                            >  2:3352333/1‑140 (MQ=255)

                          cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGGTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCgg                                                                                                            >  2:921229/1‑139 (MQ=255)

                          cggcggGAAGTTCGATTTCCACCATGCCAACAAAATAAACGGTCAGGCGTTTGATATGAAGAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGt                                                                                                            >  2:1414808/1‑140 (MQ=255)

                           ggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGACCAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGtt                                                                                                           >  2:3100266/1‑140 (MQ=255)

                           ggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGtt                                                                                                           >  1:1670967/1‑140 (MQ=255)

                           ggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCttt                                                                                                           >  2:1532956/1‑140 (MQ=255)

                           ggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGtt                                                                                                           >  1:349468/1‑140 (MQ=255)

                               ggAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCat                                                                                                       >  1:1616827/1‑140 (MQ=255)

                                 aaGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCatat                                                                                                     <  1:5146949/140‑1 (MQ=255)

                                    ttCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCa                                                                                                  >  2:4618842/1‑140 (MQ=255)

                                    ttCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATAAGAACAAGCCGATGTTTGCGGCCGCGAAAGGACAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCa                                                                                                  >  2:4718030/1‑140 (MQ=255)

                                      cGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACg                                                                                                <  1:5025907/140‑1 (MQ=255)

                                      cGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACg                                                                                                <  1:3365232/140‑1 (MQ=255)

                                           tCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                                           <  1:4637768/140‑1 (MQ=255)

                                           tCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                                           <  1:1501008/140‑1 (MQ=255)

                                             caccaTGCCAACAAAATCAACGGTCAGGCGGTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCa                                                                                         <  1:282123/140‑1 (MQ=255)

                                                  tGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTcc                                                                                    >  2:2585199/1‑140 (MQ=255)

                                                  tGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTcc                                                                                    >  2:3807273/1‑140 (MQ=255)

                                                            aTCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTc                                                                          >  1:747266/1‑140 (MQ=255)

                                                                aCGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGaa                                                                      <  2:2754477/140‑1 (MQ=255)

                                                                      aGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGc                                                                >  1:4926846/1‑140 (MQ=255)

                                                                             tGATATGAACAAGCCGATGTTTGCGGCGGCGCAAGGGCAATACGAACGTTGGGCTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGGAAACCGc                                                         >  2:858470/1‑140 (MQ=255)

                                                                                tatGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGGGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTGCCGTATCTTGTCAGAAAATGGCAAACCGCCAg                                                      <  2:2087821/140‑1 (MQ=255)

                                                                                     aCAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACATCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCt                                                 >  1:4089310/1‑140 (MQ=255)

                                                                                         gCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATc                                             >  1:5232022/1‑140 (MQ=255)

                                                                                         gCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATc                                             >  1:1075753/1‑140 (MQ=255)

                                                                                         gCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGACACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATc                                             >  1:3693699/1‑140 (MQ=255)

                                                                                            gATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATcgcg                                          <  1:2427544/140‑1 (MQ=255)

                                                                                            gATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATcgcg                                          <  1:4743543/140‑1 (MQ=255)

                                                                                              tGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCTTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATcgcgcg                                        >  2:4368617/1‑140 (MQ=255)

                                                                                              tGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATcgcgcg                                        >  2:5126203/1‑140 (MQ=255)

                                                                                                 ttGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGc                                     <  1:3898914/140‑1 (MQ=255)

                                                                                                 ttGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGc                                     <  1:4245206/140‑1 (MQ=255)

                                                                                                 ttGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGc                                     <  2:1650953/140‑1 (MQ=255)

                                                                                                 ttGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGc                                     <  2:1453589/140‑1 (MQ=255)

                                                                                                 ttGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGc                                     <  1:3619167/140‑1 (MQ=255)

                                                                                                 ttGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGc                                     <  1:5006951/140‑1 (MQ=255)

                                                                                                 ttGCGGCAGCGAGAGGGCAATACGAACGTTGGGTTATTTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGCAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGc                                     <  2:1317492/140‑1 (MQ=255)

                                                                                                   gcggcggcgAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTg                                   >  2:4866265/1‑140 (MQ=255)

                                                                                                     ggcggcgAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGa                                 <  2:1616827/140‑1 (MQ=255)

                                                                                                     ggcggcgAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGa                                 >  1:3549956/1‑140 (MQ=255)

                                                                                                         gcgAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGa                             <  1:3174095/140‑1 (MQ=255)

                                                                                                                ggCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGTCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGtt                      <  2:4895001/140‑1 (MQ=255)

                                                                                                                ggCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGtt                      <  1:3741294/140‑1 (MQ=255)

                                                                                                                ggCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGtt                      <  1:4214110/140‑1 (MQ=255)

                                                                                                                 gCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTa                     <  2:1988105/140‑1 (MQ=255)

                                                                                                                 gCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATAGCCACGGCACGCAGTTCCGGATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTa                     <  1:3275237/140‑1 (MQ=255)

                                                                                                                     tACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGt                 <  1:1977540/140‑1 (MQ=255)

                                                                                                                     tACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGt                 <  2:2992107/140‑1 (MQ=255)

                                                                                                                      aCGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTa                <  1:2291679/140‑1 (MQ=255)

                                                                                                                       cGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAg               <  2:4507725/140‑1 (MQ=255)

                                                                                                                       cGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAg               <  2:5183139/140‑1 (MQ=255)

                                                                                                                       cGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCGGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGACAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAg               <  1:5300084/140‑1 (MQ=255)

                                                                                                                          ccGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACACAGTTGCGTATCTTGTCAGAAAATGGCAAACCGCCAGCCGCTCATCCCGCTGGCTGGAAAGATACCGTTAAGGTAGAAg            >  2:4429820/2‑140 (MQ=255)

                                                                                                                          aCGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCGATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCGCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAg            >  2:3276581/1‑140 (MQ=255)

                                                                                                                          aCGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGGTACCGTTAAGGTATAAg            >  2:362941/1‑140 (MQ=255)

                                                                                                                          aCGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAg            >  1:517695/1‑140 (MQ=255)

                                                                                                                          aCGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCAGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGAAGagg            >  2:1056805/1‑138 (MQ=255)

                                                                                                                           cGTTGGGTTATCTCTGGCGTGGGCGACATGCTGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCCCGGGCTGGAAAGATACCGCCAAGGTAGAAgg           >  2:3695442/1‑140 (MQ=255)

                                                                                                                           cGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAgg           >  1:1257125/1‑140 (MQ=255)

                                                                                                                            gTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGt          <  1:4695661/140‑1 (MQ=255)

                                                                                                                            gTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGt          <  2:2132041/140‑1 (MQ=255)

                                                                                                                               gggTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTAAt       >  1:405902/1‑140 (MQ=255)

                                                                                                                               gggTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTAAt       <  2:4579787/140‑1 (MQ=255)

                                                                                                                               gggTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTAAt       >  2:3896758/1‑140 (MQ=255)

                                                                                                                                ggTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTAATg      >  2:2277786/1‑140 (MQ=255)

                                                                                                                                 gTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCGCGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCAGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTAATGt     <  2:4234978/140‑1 (MQ=255)

                                                                                                                                    aTCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATGCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGATAGAAGGTAATGTCAg  >  1:2960728/1‑140 (MQ=255)

                                                                                                                                    aTCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTAAGGTccg  >  2:4303382/1‑138 (MQ=255)

CACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTAATGTCAG  >  NZ_CP025268/138081‑138352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`