BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,455,211	T→C	T320A (ACA→GCA)	tynA ←	primary‑amine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,455,211	0	T	C	98.8%	796.1 / NA	241	T320A (ACA→GCA)	tynA	primary‑amine oxidase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (98/140); minor base G (2/1); total (100/141)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

CATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGAC  >  NZ_CP025268/1455077‑1455346

cATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCa                                                                                                                                    >  1:2188758/1‑140 (MQ=255)

 aTGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCAt                                                                                                                                   <  1:1721834/140‑1 (MQ=255)

  tGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCCCCAGTAATGGTGTAATTTTTCCCTTCAGGCTCAATGATTTGCATAGGGTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCAtt                                                                                                                                  <  1:377302/140‑1 (MQ=255)

        ggTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCAcc                                                                                                                            >  1:4775358/1‑140 (MQ=255)

          tGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTATTTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCgg                                                                                                                          <  1:1228139/140‑1 (MQ=255)

           gAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGa                                                                                                                         <  2:2991533/140‑1 (MQ=255)

           gAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGa                                                                                                                         <  1:4206999/140‑1 (MQ=255)

                 cccAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTAcc                                                                                                                   >  1:4446818/1‑140 (MQ=255)

                 cccAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTAcc                                                                                                                   <  2:2364905/140‑1 (MQ=255)

                      ttCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGAcc                                                                                                              >  1:4892457/1‑140 (MQ=255)

                         cGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACcttc                                                                                                           >  2:594527/1‑140 (MQ=255)

                           ccAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAAGGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACcttctt                                                                                                         >  1:2982389/1‑140 (MQ=255)

                           ccAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACcttctt                                                                                                         <  1:1176997/140‑1 (MQ=255)

                                    cATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTaa                                                                                                <  2:941619/140‑1 (MQ=255)

                                      tatCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACg                                                                                              >  2:1831697/1‑140 (MQ=255)

                                              gaaaTGGTGTAATTTTGACGTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGTCATCAAATGGTCGTGGGGTCATCGGCACCGGAACTACCGGACTTTCTTCAAACTTAACGAtttgttt                                                                                      >  2:2496452/3‑140 (MQ=255)

                                              gTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt                                                                                      <  1:1116287/140‑1 (MQ=255)

                                              gTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt                                                                                      <  1:5252660/140‑1 (MQ=255)

                                              gTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt                                                                                      <  1:3132201/140‑1 (MQ=255)

                                              gTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt                                                                                      <  1:1424629/140‑1 (MQ=255)

                                              gTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt                                                                                      >  2:1429350/1‑140 (MQ=255)

                                              gTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttttt                                                                                      <  1:1040515/140‑1 (MQ=255)

                                               tAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTc                                                                                     <  1:4393541/140‑1 (MQ=255)

                                               tAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTc                                                                                     <  2:2601552/140‑1 (MQ=255)

                                                      gtAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTCACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTa                                                                              >  1:192667/1‑140 (MQ=255)

                                                         aTTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGAc                                                                                                               <  2:571755/104‑1 (MQ=255)

                                                         aTTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGAc                                                                                                               >  1:571755/1‑104 (MQ=255)

                                                         aTTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAAt                                                                           >  2:3701547/1‑140 (MQ=255)

                                                               aCCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGa                                                                     >  2:1858598/1‑140 (MQ=255)

                                                                ccTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGAc                                                                    <  1:3037961/140‑1 (MQ=255)

                                                                   tCAGGCTGAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGc                                                                 <  2:4683293/140‑1 (MQ=255)

                                                                   tCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGc                                                                 >  1:1996848/1‑140 (MQ=255)

                                                                    cAGGCTCAATGATTTGCATAGGCTTAACTGCCGTAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTGTTTTCTGTTCTAAATCAACGACCGcc                                                                >  1:2674490/1‑140 (MQ=255)

                                                                    cAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGcc                                                                >  1:2369963/1‑140 (MQ=255)

                                                                    cAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGcc                                                                >  1:2470318/1‑140 (MQ=255)

                                                                      ggCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATTAAAAGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGccac                                                              <  1:3805729/140‑1 (MQ=255)

                                                                      ggCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGccac                                                              <  2:4129479/140‑1 (MQ=255)

                                                                             tGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGttt                                                       <  1:2225638/140‑1 (MQ=255)

                                                                             tGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGttt                                                       <  2:2485260/140‑1 (MQ=255)

                                                                                tttGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTTACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCg                                                    <  1:4573762/140‑1 (MQ=255)

                                                                                 ttGCATGGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTGGa                                                   >  2:4262654/1‑140 (MQ=255)

                                                                                 ttGCATAGGCTTAACTGCTGGAGCAATGCGGTCACGGCCAGGAAATGGGCGTGCGGTCATTGGCGCCGGAACTACCGGACTGTCTTCAGTCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGGTTTCGa                                                   >  2:1153221/1‑140 (MQ=255)

                                                                                 ttGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGa                                                   >  1:3493927/1‑140 (MQ=255)

                                                                                 ttGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGa                                                   >  1:3413543/1‑140 (MQ=255)

                                                                                 ttGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGa                                                   >  1:2821274/1‑140 (MQ=255)

                                                                                  tGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGAt                                                  <  1:756699/140‑1 (MQ=255)

                                                                                  tGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGAt                                                  >  1:4294084/1‑140 (MQ=255)

                                                                                  tGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGAt                                                  >  2:4868870/1‑140 (MQ=255)

                                                                                  tGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTCAACGATTTTTTTCTGTTCTAAATCAACGCCCGCCACCAGGTTTTCGAt                                                  <  2:3532575/140‑1 (MQ=255)

                                                                                  ggCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGAt                                                  <  1:4728702/139‑1 (MQ=255)

                                                                                   gCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATg                                                 >  1:2102579/1‑140 (MQ=255)

                                                                                     aTAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATggg                                               <  1:4862835/140‑1 (MQ=255)

                                                                                       aGGCTTAAGTGGCGGGGCAACGCGGTCACGGCCATAAAATGGGCGTGCGGTCATTGGCACCGGCACTACCGGACCTTCTTCAATCTTAACGAGTTTTTTCTGTTCTAAATCAACGACCGCCCCCAGGGTTTCGAGGGGAt                                             <  2:3989966/140‑1 (MQ=255)

                                                                                       aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTTCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTACCGATTTTTTTCTGTTCTAAATCAACGAGCGCCACCAGGTTTTCGATGGGAt                                             <  2:2073066/140‑1 (MQ=255)

                                                                                       aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAt                                             <  2:3795060/140‑1 (MQ=255)

                                                                                       aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAt                                             <  2:3982277/140‑1 (MQ=255)

                                                                                       aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAt                                             <  2:987262/140‑1 (MQ=255)

                                                                                       aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAt                                             <  1:25915/140‑1 (MQ=255)

                                                                                       aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAt                                             <  1:620916/140‑1 (MQ=255)

                                                                                       aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAt                                             <  1:3344167/140‑1 (MQ=255)

                                                                                       aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAt                                             <  1:1852226/140‑1 (MQ=255)

                                                                                       aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAt                                             <  1:2748379/140‑1 (MQ=255)

                                                                                       aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAt                                             <  1:4156166/140‑1 (MQ=255)

                                                                                       aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAt                                             >  1:3154820/1‑140 (MQ=255)

                                                                                       aGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATAACATGGGCGTGCGGTCATTGGCACCGGAACTCCCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGCCCGCCGCCAGGTTTTCGATGGGAt                                             <  1:2027477/140‑1 (MQ=255)

                                                                                         gCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCGACCAGGTTTTGCATGCGAtgg                                           >  2:3432834/1‑139 (MQ=255)

                                                                                         gCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAtgt                                           >  1:4973656/1‑140 (MQ=255)

                                                                                         gCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAtgt                                           >  1:3480555/1‑140 (MQ=255)

                                                                                          cTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAtgtg                                          <  2:1319711/140‑1 (MQ=255)

                                                                                          attAACTGCCGGAGCAACGCGCTCACGGGCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAtgtg                                          <  2:2666480/139‑1 (MQ=255)

                                                                                            taccaaccGGATCATCGCGGTCACCGCCAGTAAATGGGCGTGCGGTCCTTGGGAGCGGCACTTCCGGACATTCTTCAAGTGTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCTGGTTTTCGATGGGGTGTGcc                                        <  2:5094355/134‑1 (MQ=255)

                                                                                            tAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGcc                                        <  1:369543/140‑1 (MQ=255)

                                                                                            tAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGcc                                        <  2:5257318/140‑1 (MQ=255)

                                                                                            tAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGcc                                        <  1:3250100/140‑1 (MQ=255)

                                                                                              aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCa                                      >  1:120156/1‑140 (MQ=255)

                                                                                                 gCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCagta                                   <  1:3575533/140‑1 (MQ=255)

                                                                                                   cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCagtagt                                 >  2:480793/1‑140 (MQ=255)

                                                                                                   cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCagtagt                                 >  1:2325826/1‑140 (MQ=255)

                                                                                                   cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCagtagt                                 >  2:4889183/1‑140 (MQ=255)

                                                                                                   cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCagtagt                                 >  1:1312731/1‑140 (MQ=255)

                                                                                                   cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCagtagt                                 >  1:67605/1‑140 (MQ=255)

                                                                                                   cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCagtagt                                 >  1:3887724/1‑140 (MQ=255)

                                                                                                     gAGCAACGCGGTCACGGCCATCAAATGCGCGTGCGGTCATAGGCACCCGAACTCCCCGAACTTCTTCAATCTTAACGATTTTTTCCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTg                               <  2:4636148/140‑1 (MQ=255)

                                                                                                                tCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGAc                    <  1:893580/140‑1 (MQ=255)

                                                                                                                tCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGAc                    <  1:2614132/140‑1 (MQ=255)

                                                                                                                tCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGAc                    <  2:457318/140‑1 (MQ=255)

                                                                                                                tCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGAc                    <  2:3274939/140‑1 (MQ=255)

                                                                                                                tCACGGACATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCCACGACCGCCACCAGGTTTTCGATAGGATGTGCCCAGTAGTTGCCATCACCGAc                    <  2:1969744/140‑1 (MQ=255)

                                                                                                                  aCGGCTATCAAACGCGCGTGCGGTTATTGGCACCCGAACTACCGGACCTTCTTCAATCTTAGCGCTTTTTTTCTGTTCTAAATCAACCACCGCCACCAGGGTCTCGATGGGATGTGCTCAGTAGTTGCCATCACCGAcct                  <  2:4140352/140‑3 (MQ=255)

                                                                                                                       cATCACATGCGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGa             <  2:2956779/140‑1 (MQ=255)

                                                                                                                       cATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGa             <  1:5160809/140‑1 (MQ=255)

                                                                                                                       cATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATGTTTTTTGTTTCTAAATCAACGACGGCGACCAGGTTTTCGATGGGATGTGCCCGGTAGTTGCCAGCACGGACATCAAGa             >  2:1695877/1‑140 (MQ=255)

                                                                                                                       cATCAAATGGGCGTGCGGTCATTGGCAACGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGa             <  2:1937656/140‑1 (MQ=255)

                                                                                                                       cATCAAATGGACGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCTCAGTAGTTGCCATCACCGACATCAAGa             <  2:3917247/140‑1 (MQ=255)

                                                                                                                              tGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtga      <  1:4342565/140‑1 (MQ=255)

                                                                                                                               gggcgggcGGTCATTGGCCCCGGAACTACCGGACCGTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGCTGGGATGAGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtgat     <  2:5163762/140‑1 (MQ=255)

                                                                                                                               gggCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtgat     <  2:3560899/140‑1 (MQ=255)

                                                                                                                                ggCGTGCGGTCATTGGCACCGGAACTTCCGAACCTTCTTCAATCTTAACGAATTTTTTCTGTTCTAAATCAAGGATCGCCACCAGGTTTTGGATGGGCTGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtgatg    >  1:3193816/1‑140 (MQ=255)

                                                                                                                                ggCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtgatg    >  2:3402264/1‑140 (MQ=255)

                                                                                                                                 gCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtgatga   <  1:3964164/140‑1 (MQ=255)

CATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGAC  >  NZ_CP025268/1455077‑1455346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`