BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,469,308	A→G	317 (TAA→TAG) N7D (AAC→GAC)	paaX → paaY →	phenylacetic acid degradation operon negative regulatory protein PaaX phenylacetic acid degradation protein PaaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,469,308	0	A	G	99.6%	758.3 / NA	232	317 (TAA→TAG) N7D (AAC→GAC)	paaX paaY	phenylacetic acid degradation operon negative regulatory protein PaaX phenylacetic acid degradation protein PaaY
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (85/146); minor base C (0/1); total (85/147)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 232 total aligned reads displayed.

GCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGT  >  NZ_CP025268/1469171‑1469444

gcgcCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCAGGGAAGCCTGTATTTTCAACGTTTTGGCGGATTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGAc                                                                                                                                        <  2:1230183/140‑1 (MQ=255)

      gctgctTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTTAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTg                                                                                                                                  >  2:3229331/1‑140 (MQ=255)

      gctgctTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTg                                                                                                                                  >  1:43696/1‑140 (MQ=255)

       ctgctTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGa                                                                                                                                 >  1:2777630/1‑140 (MQ=255)

             tAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCgg                                                                                                                           >  1:2337216/1‑140 (MQ=255)

              aGCGTTCGTTCGTGAAAACGGTGAAACCTCGGTCGATGAACTGCCTGCGCCGGGAAGCCCGTATTTTCACCGTTTTGGCGGCTTCAATATTGATCAGGAGGCGTTATGCCAATTTATCAGTTCGCCGGTCTGACTCCGGt                                                                                                                          <  2:609837/140‑1 (MQ=255)

              aGCGTTCGTTAGTGAAAAAGGTGAATCCTCGGTCTGGCAACTGCCTACGCCAGGAAACCTGTATTTTCAACGTTTTGGCGGCTTGAATAATGAAGAGAAGGCGTTATGACAATCTATCAGATAGACGGTCTGGCTCCGGt                                                                                                                          <  2:3674034/140‑1 (MQ=255)

              aGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGt                                                                                                                          <  2:2777630/140‑1 (MQ=255)

                  ttcgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAGCGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTtgtg                                                                                                                      >  1:4237343/1‑140 (MQ=255)

                   tcgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGc                                                                                                                     <  1:2855299/140‑1 (MQ=255)

                    cgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGcc                                                                                                                    >  1:2520610/1‑140 (MQ=255)

                     gttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCa                                                                                                                   <  1:1422027/140‑1 (MQ=255)

                     gttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCa                                                                                                                   <  1:2359268/140‑1 (MQ=255)

                      ttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCag                                                                                                                  >  1:1175439/1‑140 (MQ=255)

                         gTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCagaag                                                                                                               <  1:3414925/140‑1 (MQ=255)

                          tGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCagaaga                                                                                                              <  2:4356917/140‑1 (MQ=255)

                                 ggTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGtttt                                                                                                       >  1:3650951/1‑140 (MQ=255)

                                   tGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGt                                                                                                     <  1:3258213/140‑1 (MQ=255)

                                    gAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTc                                                                                                    >  2:2271629/1‑140 (MQ=255)

                                    gAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTc                                                                                                    <  2:400661/140‑1 (MQ=255)

                                    gAAACCTCGGTCGGTGAACTGCCTGCGCAGGGAAGCCTGTATTTTCAACGTTTTGTCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTTTc                                                                                                    >  2:1699375/1‑140 (MQ=255)

                                      aaCCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCa                                                                                                  <  1:1632244/140‑1 (MQ=255)

                                      aaCCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCa                                                                                                  <  2:4816158/140‑1 (MQ=255)

                                         cTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATcc                                                                                               <  2:1399109/140‑1 (MQ=255)

                                         cTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATcc                                                                                               <  1:4022023/140‑1 (MQ=255)

                                           cggtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGa                                                                                             <  2:1789144/140‑1 (MQ=255)

                                             gtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACa                                                                                           <  2:3048761/140‑1 (MQ=255)

                                             gcaggtTAACTGCCGGCTCCGGGAAGGCTTTATTTTGAACGTTCTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAAGTTATCATATACACGGCCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTACAGCCGCCa                                                                                           <  2:4207074/137‑1 (MQ=255)

                                              ttggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                          <  2:152823/138‑1 (MQ=255)

                                              tcggtGAACTGCCTGCTCCGGGAAGCCTCTATTTTCAACGATTTGGCGGCTTGAATATTGAACAGGAGGCGTTTTGCCAGTTTATCAGATAGACGGTCTGGCTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                          <  2:4428662/140‑1 (MQ=255)

                                              tcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                          <  2:3093629/140‑1 (MQ=255)

                                              tcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                          <  1:3896457/140‑1 (MQ=255)

                                              tcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                          <  2:5315023/140‑1 (MQ=255)

                                              tcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                          <  2:873618/140‑1 (MQ=255)

                                              tcggtGAACTGACTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGTCGGCCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                          <  2:5000721/140‑1 (MQ=255)

                                                 gtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCgg                                                                                       >  1:4598328/1‑140 (MQ=255)

                                                   gtaCTGCCTGCGCCGTGAAGCCTGTACTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTCATGCCCATTTATCAGCTAGACGGTCTGACTCCGGTTGTGCCCGCAGAGAGTCTTGTCCCTCCGACCGCGGTa                                                                                     <  2:3520265/138‑1 (MQ=255)

                                                    aaCTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGCGGCGTTATGGCAATTTATCAGATAGACGGTCTGACTCCGGTGGTGGCAGAAGAGAGTTTTGTCCATCCGAGAGCGGTAt                                                                                    >  2:2923494/1‑140 (MQ=255)

                                                           tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                             <  1:4732488/140‑1 (MQ=255)

                                                           tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                             <  2:1159536/140‑1 (MQ=255)

                                                           tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                             >  1:828578/1‑140 (MQ=255)

                                                           tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                             <  2:1944/140‑1 (MQ=255)

                                                           tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                             <  1:1395751/140‑1 (MQ=255)

                                                           ggcgcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTCTCAGGTAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                             <  2:3146696/139‑1 (MQ=255)

                                                           cgcgcGGGGAGGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGACCAGGAGGCGTTAGGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTCCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                             <  2:2663251/139‑1 (MQ=255)

                                                            gcgcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGc                                                                            >  2:2567911/1‑140 (MQ=255)

                                                            gcgcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGc                                                                            >  1:3186636/1‑140 (MQ=255)

                                                            gcgcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGc                                                                            >  1:4103160/1‑140 (MQ=255)

                                                              gcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGa                                                                          >  1:3544574/1‑140 (MQ=255)

                                                                cGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATg                                                                        <  1:3698815/140‑1 (MQ=255)

                                                                 gggAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGCTCGTCGATGt                                                                       >  1:2191480/1‑140 (MQ=255)

                                                                  ggAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGtt                                                                      >  2:631043/1‑140 (MQ=255)

                                                                   gAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTa                                                                     >  2:1895442/1‑140 (MQ=255)

                                                                     agacTGTAATTTCAACGTGTTGTCGTGTTGAATATTGAACAGGAGGCGTTAAGCCAATTTCTCAGATAGACGGTCTGCCGCCGGTTGCGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTAtt                                                                   <  2:4841449/137‑1 (MQ=255)

                                                                     aGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTAtt                                                                   <  2:4162512/140‑1 (MQ=255)

                                                                     aGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTACGGTTGTGCCAGAAGAGAGTTCTGTCCATCCGACAGCGGTATTGATCGGCGATGTTAtt                                                                   <  1:4906496/140‑1 (MQ=255)

                                                                       ccTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATtct                                                                 >  1:3548665/1‑140 (MQ=255)

                                                                             ttttCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCaa                                                           <  1:2647185/140‑1 (MQ=255)

                                                                              tttCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGGCGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAg                                                          <  1:2276956/140‑1 (MQ=255)

                                                                                 cAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGc                                                       >  1:1667832/1‑140 (MQ=255)

                                                                                  aaCGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGACAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCg                                                      >  2:1179760/1‑140 (MQ=255)

                                                                                     gTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGttt                                                   >  2:807820/1‑140 (MQ=255)

                                                                                       tttGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTAc                                                 >  2:3728524/1‑140 (MQ=255)

                                                                                       tttGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTAc                                                 >  1:642087/1‑140 (MQ=255)

                                                                                       tttGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTAc                                                 >  1:4345386/1‑140 (MQ=255)

                                                                                         tGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGt                                               <  1:4975371/140‑1 (MQ=255)

                                                                                           gcgtcTTGAATATTGAACAGGAGGAGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTg                                             <  1:4248907/140‑1 (MQ=255)

                                                                                               cTTGAATATTGAACAGGAGGCTTTATGCTCATTTATCAGATAGACGGTCTGCCTCGCCTTGTGCCAGAAGAGAGTTTTGTCCTGCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGcc                                         <  2:2073465/140‑1 (MQ=255)

                                                                                               cTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCGAGGGCGTTTACGTTGGGcc                                         <  1:1761233/140‑1 (MQ=255)

                                                                                               cTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGcc                                         <  1:1231795/140‑1 (MQ=255)

                                                                                               cTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGcc                                         <  2:4232194/140‑1 (MQ=255)

                                                                                               cTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGcc                                         <  1:3396954/140‑1 (MQ=255)

                                                                                               cTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGcc                                         <  1:2020296/140‑1 (MQ=255)

                                                                                                             aggaggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGc                           >  2:3463936/1‑140 (MQ=255)

                                                                                                             aggaggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGc                           >  1:951899/1‑140 (MQ=255)

                                                                                                               gaggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGt                         >  1:3480990/1‑140 (MQ=255)

                                                                                                               gaggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGGTTTTTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGt                         >  2:4318780/1‑140 (MQ=255)

                                                                                                                aggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTg                        <  1:3222234/140‑1 (MQ=255)

                                                                                                                    gTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGa                    >  1:5151607/1‑140 (MQ=255)

                                                                                                                    gTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGa                    >  1:311870/1‑140 (MQ=255)

                                                                                                                      tATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGAtt                  <  1:5305514/140‑1 (MQ=255)

                                                                                                                      tATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGAtt                  >  1:2067213/1‑140 (MQ=255)

                                                                                                                       aTGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGAttt                 <  2:4499986/140‑1 (MQ=255)

                                                                                                                       aTGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGAttt                 >  2:5205883/1‑140 (MQ=255)

                                                                                                                          ccAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTgg              <  2:2580617/140‑1 (MQ=255)

                                                                                                                            aaTTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTc            <  2:1659677/140‑1 (MQ=255)

                                                                                                                                tATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTAt        <  2:379318/140‑1 (MQ=255)

                                                                                                                                tATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTAt        <  2:2640126/140‑1 (MQ=255)

                                                                                                                                tATCAGATAGACGGTCCGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCCGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTAt        <  1:2947469/140‑1 (MQ=255)

                                                                                                                                 aTCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATc       <  1:807707/140‑1 (MQ=255)

                                                                                                                                  tCATATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTAGCGATTTTGGTCGTATCg      <  2:652467/140‑1 (MQ=255)

                                                                                                                                  tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACCGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg      <  2:4690753/140‑1 (MQ=255)

                                                                                                                                  tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg      <  2:2919031/140‑1 (MQ=255)

                                                                                                                                  tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg      <  2:883828/140‑1 (MQ=255)

                                                                                                                                  tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg      <  1:3712904/140‑1 (MQ=255)

                                                                                                                                  tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg      <  1:385435/140‑1 (MQ=255)

                                                                                                                                  tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg      <  1:3916819/140‑1 (MQ=255)

                                                                                                                                  tCAGATAGACGGTCTGACTCCGGGTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg      <  2:3272841/140‑1 (MQ=255)

                                                                                                                                   cAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCgt     >  1:3595027/1‑140 (MQ=255)

                                                                                                                                      aTAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCgtggt  <  2:5237917/140‑1 (MQ=255)

GCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGT  >  NZ_CP025268/1469171‑1469444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`