BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,496,208	G→A	R163K (AGG→AAG)	cybB →	cytochrome b561

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,496,208	0	G	A	99.5%	704.9 / NA	191	R163K (AGG→AAG)	cybB	cytochrome b561
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (39/150); total (39/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

ACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATATT  >  NZ_CP025268/1496072‑1496338

aCGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCAGTGGCACACCACTATTTCTGGAAGGa                                                                                                                                 <  2:4784518/140‑1 (MQ=255)

aCGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGa                                                                                                                                 <  1:1007251/140‑1 (MQ=255)

aCGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGa                                                                                                                                 <  1:2453564/140‑1 (MQ=255)

aCGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGa                                                                                                                                 <  2:4080934/140‑1 (MQ=255)

   aTGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGTAGCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTATGGAAGGacaa                                                                                                                              <  1:4431944/140‑1 (MQ=255)

     gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                            <  2:2231364/140‑1 (MQ=255)

     gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                            <  2:4015075/140‑1 (MQ=255)

     gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                            <  1:4385413/140‑1 (MQ=255)

     gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                            <  1:4345356/140‑1 (MQ=255)

     gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                            <  1:3546862/140‑1 (MQ=255)

     gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                            <  2:653937/140‑1 (MQ=255)

     gCCGTACGATTCATAGGCCAGATTCGAAAGCTGAAATAGCTTAAAGTCCTGGCGTTAAACGCTGGTGAATCTGGGATAGTTTGTCCTCGGCTTGCGCGCTGCGGCTGCCCTGGCACACCACTATTTCTACAAGGacacca                                                                                                                            <  2:805833/140‑1 (MQ=255)

       cTTACGCTTCTGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCTTCGGGTTGCACGCTGCGGCTGCACTGGCATACCACTATTTCTGGAAGGACAacaca                                                                                                                          <  2:926798/140‑1 (MQ=255)

       cTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAacaca                                                                                                                          <  1:1475490/140‑1 (MQ=255)

       cTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAacaca                                                                                                                          <  1:3110877/140‑1 (MQ=255)

        ttACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAacacac                                                                                                                         <  1:419681/140‑1 (MQ=255)

          aCGCTTCAGAGGCCAATTCCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACtt                                                                                                                       <  2:5037125/140‑1 (MQ=255)

            gCTTCAGAGGCGAAGATCTAACGTGTAGATGGCTTAAAGTAGTGGAATGAAACGCTGGAGAGTCTGAGAAATTTTGTCATCGGGTTGCACGCTGCTGCGTCACTGGAACACCACTAGTGCTCGAAGGACAACCCACttat                                                                                                                     <  2:1671143/140‑3 (MQ=255)

            gCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCt                                                                                                                     <  1:3583474/140‑1 (MQ=255)

               tCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCTGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACg                                                                                                                  >  2:1468899/1‑140 (MQ=255)

                      ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTTCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                           <  1:3906094/140‑1 (MQ=255)

                      ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                           <  2:3082796/140‑1 (MQ=255)

                      ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                           <  1:2495855/140‑1 (MQ=255)

                      ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                           <  1:2395436/140‑1 (MQ=255)

                      ccAATTTCGAACGGCTCGATAGCTTAAATTCGTGGCATGAGACGCTGGCGAATCTGGGATATTATGTCATCGGCTTGCATGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGAGAACACACTTCTACGCatgatc                                                                                                           <  2:4431870/140‑2 (MQ=255)

                      ccAATTTCGAACAGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                           <  1:4578814/140‑1 (MQ=255)

                        aaTTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGcc                                                                                                         >  2:1571785/1‑140 (MQ=255)

                        aaTTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGcc                                                                                                         >  2:404290/1‑140 (MQ=255)

                          tttCGACCGGGTAGATAGCTTACAGTCGTAACATGAAACGCTGGCGACTCTAGGATATTTTGTCATCGGGTTGCACCCTGCGGCTGCACTGGAACACCACTATTACTGGAAGGACAACACACTTCTACGCATGCTGCcgc                                                                                                       <  2:4214964/140‑1 (MQ=255)

                               aaCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTaaa                                                                                                  <  2:4891565/140‑1 (MQ=255)

                                 cGGGTAGATATCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACGATGTCTGGAAGGACAACACACTTCCACGCATGATGCCGCGTAAACg                                                                                                <  2:3899153/140‑1 (MQ=255)

                                 cGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACg                                                                                                <  1:1088826/140‑1 (MQ=255)

                                    gTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTc                                                                                             >  1:5053929/1‑140 (MQ=255)

                                        aTAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGa                                                                                         <  2:2473218/140‑1 (MQ=255)

                                          aGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGCGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGAAAACCCACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAg                                                                                       <  1:1951331/140‑1 (MQ=255)

                                            cTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGa                                                                                     <  2:3756323/140‑1 (MQ=255)

                                              tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGata                                                                                   >  1:3950721/1‑140 (MQ=255)

                                              tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGata                                                                                   >  1:1036447/1‑140 (MQ=255)

                                               aaaGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGatat                                                                                  >  1:84780/1‑140 (MQ=255)

                                               aaaGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGatat                                                                                  >  1:4970356/1‑140 (MQ=255)

                                                      tGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGa                                                                           >  2:1990341/1‑140 (MQ=255)

                                                      tGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGa                                                                           >  1:3522589/1‑140 (MQ=255)

                                                      tGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGa                                                                           >  1:4929384/1‑140 (MQ=255)

                                                      tGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGa                                                                           >  2:4585727/1‑140 (MQ=255)

                                                         cATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGaaaa                                                                        <  2:4783890/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                    <  2:5091735/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                    <  1:2062357/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                    <  1:1891727/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCCGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                    <  1:3163360/140‑1 (MQ=255)

                                                                  cTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTAc                                                               <  1:4307751/140‑1 (MQ=255)

                                                                  cTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGACTGCACCGGCACACCACTATTTCTCGAAGGACAACACACTTCTACTCATGATGCCGCGTAAACGTTTCTGAAGTATATTTAAAGAAAACGCCTGTAc                                                               <  1:3198157/140‑1 (MQ=255)

                                                                          tCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                       <  1:3913950/140‑1 (MQ=255)

                                                                          tCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                       <  2:3598096/140‑1 (MQ=255)

                                                                          tCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                       <  1:614787/140‑1 (MQ=255)

                                                                          tCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                       <  1:1154768/140‑1 (MQ=255)

                                                                             gggATATTTTGTCATCGGGTTGCACGCTGCGGCTGCTCTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGAcc                                                    <  1:2984392/140‑1 (MQ=255)

                                                                             gggATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGAcc                                                    <  1:2818528/140‑1 (MQ=255)

                                                                               gATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCg                                                  <  1:1843118/140‑1 (MQ=255)

                                                                               gATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAAGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCg                                                  <  1:4446272/140‑1 (MQ=255)

                                                                                    tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                             <  1:1373278/140‑1 (MQ=255)

                                                                                    tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                             <  1:2638455/140‑1 (MQ=255)

                                                                                    tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGGTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                             <  1:3872512/140‑1 (MQ=255)

                                                                                    tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTAAGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                             <  2:4534796/140‑1 (MQ=255)

                                                                                          ttCGTGTTGCGCGCTGCGGCGGGACTGGCACACCAGTATTTCCGGAGGCACATTACACTGCGAAGCATGATGACGCGTAAACGTTCCTGAAGGATATTTAACGAAAACGTCTGGTCTAATCCCGGCCCGTGGTCCAGGCg                                       <  2:2453153/139‑1 (MQ=255)

                                                                                          ttCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTTCGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg                                       <  1:4724950/139‑1 (MQ=255)

                                                                                          aTCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg                                       <  2:453712/140‑1 (MQ=255)

                                                                                          aTCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg                                       <  2:1301820/140‑1 (MQ=255)

                                                                                          aTCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg                                       <  2:282161/140‑1 (MQ=255)

                                                                                          aTCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTGCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg                                       >  1:1241919/1‑140 (MQ=255)

                                                                                          aTCGCGTTGCACGCTGCGGCTGCACTGGCACACAACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGAATATTTAAAGAAAACGCCTGTACTACTGCCGACCCGTGGTACAgggg                                       <  2:3995165/140‑3 (MQ=255)

                                                                                             gggTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTTGAAGGACAACACACTTCTACACATGATACCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCgaag                                    >  2:469845/1‑140 (MQ=255)

                                                                                               gTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCgaagaa                                  <  1:1571785/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                <  1:3044015/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                <  1:1763158/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                <  1:5156008/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                <  2:1102174/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                <  2:3271091/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                <  2:3441102/140‑1 (MQ=255)

                                                                                                  gCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATAc                               <  1:2455518/140‑1 (MQ=255)

                                                                                                  gCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATAc                               <  1:751404/140‑1 (MQ=255)

                                                                                                    aCGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACgg                             <  1:3239452/140‑1 (MQ=255)

                                                                                                       cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                          <  2:5169424/140‑1 (MQ=255)

                                                                                                       cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                          <  1:2891500/140‑1 (MQ=255)

                                                                                                       cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                          <  1:1845935/140‑1 (MQ=255)

                                                                                                       cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                          <  1:939703/140‑1 (MQ=255)

                                                                                                       cTGCGGCTGCACTGGCACACCACTAGTTCTGGAAGGACAACACTCTTCTACACATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                          <  1:4874631/140‑1 (MQ=255)

                                                                                                        tGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCt                         >  2:468552/1‑140 (MQ=255)

                                                                                                         gCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTa                        >  1:1538184/1‑140 (MQ=255)

                                                                                                                 aCTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGaa                <  2:4929384/140‑1 (MQ=255)

                                                                                                                 aCTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGaa                <  2:3620106/140‑1 (MQ=255)

                                                                                                                  cTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAg               <  1:1272106/140‑1 (MQ=255)

                                                                                                                  cTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAg               >  1:3260107/1‑140 (MQ=255)

                                                                                                                    ggCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgcg             <  1:5140092/140‑1 (MQ=255)

                                                                                                                     gCACCCCACTTTTTCTGGTCCGACAACACACTTCTACGCATGACGCCGCGTAAACGTTCCTCAAGGATATTTAAAGACAACGCCTGTACTAATACCGACCCGTCGTACAGGCGAAGAATACGGGTCTACATCGGAAgcgc            <  2:4195589/140‑1 (MQ=255)

                                                                                                                     gCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgcgc            <  1:3097813/140‑1 (MQ=255)

                                                                                                                        caccacTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCta         <  2:239866/140‑1 (MQ=255)

                                                                                                                          ccacTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                       <  1:2243591/92‑1 (MQ=255)

                                                                                                                          ccacTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                       >  2:2243885/1‑92 (MQ=255)

                                                                                                                            acTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCtattat     <  2:5303962/140‑1 (MQ=255)

                                                                                                                             cTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATtata    >  1:2836231/1‑140 (MQ=255)

ACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATATT  >  NZ_CP025268/1496072‑1496338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`