Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 159,854 G→A F20F (TTC→TTT)  gluQRS ← tRNA glutamyl‑Q(34) synthetase GluQRS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268159,8540GA99.7% 1290.5 / NA 342F20F (TTC→TTT) gluQRStRNA glutamyl‑Q(34) synthetase GluQRS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (141/200);  minor base C (1/0);  total (142/200)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 342 total aligned reads displayed.

TCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCA  >  NZ_CP025268/159720‑159986
                                                                                                                                      |                                                                                                                                    
  cAGCTGGCGCAGGCTAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTACCGGCCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGATAGCCAAAATGaa                                                                                                                               >  2:310838/1‑140 (MQ=255)
  cAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTAGGCGTGCCAGCCAGCGACCTTGCCGGGGGCGAGCCTGCAAATAGCGGCCGAGCGCGGCGGTCAGAGAGCCAAAAGGaa                                                                                                                               >  2:1280794/1‑140 (MQ=255)
    gCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGc                                                                                                                             >  2:2742499/1‑140 (MQ=255)
    gCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGc                                                                                                                             <  1:1804041/140‑1 (MQ=255)
        gcgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGc                                                                                                                         <  2:1931783/140‑1 (MQ=255)
         cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGcc                                                                                                                        >  1:3242059/1‑140 (MQ=255)
         cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGcc                                                                                                                        <  1:4113458/140‑1 (MQ=255)
         cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGcc                                                                                                                        >  1:4808709/1‑140 (MQ=255)
         cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGcc                                                                                                                        >  1:505712/1‑140 (MQ=255)
         cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGc                                                                                                                         >  1:3300192/1‑139 (MQ=255)
         cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCGTCTATGCGGACCAGCCAGCGACCTTGCCGTGCGCGATCCGGCAAATAGCTGCCGAGCGCGGCGATCAGAGCGCCAAAATGAAGCTCGca                                                                                                                        >  2:2676456/1‑139 (MQ=255)
              gATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAg                                                                                                                   <  2:2311443/140‑1 (MQ=255)
                 aGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGgag                                                                                                                <  1:3883440/140‑1 (MQ=255)
                   tttCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGgaggg                                                                                                              >  2:1114790/1‑138 (MQ=255)
                   tttCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGagag                                                                                                              >  1:2728761/1‑140 (MQ=255)
                        gCGGCACCGGGACCTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGc                                                                                                         >  2:665520/1‑140 (MQ=255)
                        gCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGc                                                                                                         >  1:1062178/1‑140 (MQ=255)
                        gCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGc                                                                                                         >  1:3737604/1‑140 (MQ=255)
                        gCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGc                                                                                                         >  2:4994338/1‑140 (MQ=255)
                        gCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGc                                                                                                         >  2:89788/1‑140 (MQ=255)
                        gCGGCACCGGGAACTTCACGAGGCGGGCCGATAGCTTCTATGGGTACCAGCCCGCGACCTTGTCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGTACCGAGGGCCAAAATGCAGCTCGCCGGAATGAGAagggca                                                                                                         >  2:4124920/1‑138 (MQ=255)
                        gCGGCACCGGGAACTTCACGAGGCGGGACGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGc                                                                                                         >  2:472383/1‑140 (MQ=255)
                           gCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCTTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTAGCCGGAAGGAGAGGGGGCGag                                                                                                      <  2:4433950/140‑2 (MQ=255)
                           gCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                      <  1:289536/140‑1 (MQ=255)
                           gCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                      <  2:3999819/140‑1 (MQ=255)
                           ccACCGGGAACTGCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                      <  1:451625/139‑1 (MQ=255)
                             cccGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:4871372/139‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  2:3253971/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:3668216/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  2:2584889/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:1160296/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:4571457/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  2:1499102/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:2361859/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:2169013/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:516409/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGATGCCGAGCGCGGCGATCAGAGAGCCAAAATAAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:2566199/140‑1 (MQ=255)
                               cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                  <  1:498471/140‑1 (MQ=255)
                               cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                  <  2:3877178/140‑1 (MQ=255)
                               cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                  <  2:2543595/140‑1 (MQ=255)
                               cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                  <  1:1964746/140‑1 (MQ=255)
                                 ggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGTCCTTGTCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGcc                                                                                                >  2:2971223/1‑140 (MQ=255)
                                 ggAACATCACGACGCGGGTCGATATCTTCTATGAGTACCAGCCAGCGACCTTGCCGCGCGCGAGACTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAAGGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGcc                                                                                                <  2:1678568/140‑1 (MQ=255)
                                     cTTCACGAGGCGGGTCGATATTTTCTATGCGTACCAGCCAGCGACCTTGCCCGGCGCGAGCGTTCAAATAGCTGGCTACCGCGGCGCCCAGAGAGCCAAAATGAAGCTCGCCGGAAGTAGAGCGGGCGAAGCGGCCAata                                                                                            <  2:2026084/140‑1 (MQ=255)
                                     cTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGTCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAata                                                                                            <  2:4281822/140‑1 (MQ=255)
                                     cTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAata                                                                                            <  2:5265265/140‑1 (MQ=255)
                                     cTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAata                                                                                            <  1:1675722/140‑1 (MQ=255)
                                         aCGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATAcg                                                                                        >  2:3391822/1‑139 (MQ=255)
                                         aCGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACt                                                                                        <  2:3734995/140‑1 (MQ=255)
                                         aCGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCCGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACt                                                                                        <  1:4459860/140‑1 (MQ=255)
                                          cGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACtg                                                                                       <  1:4994338/140‑1 (MQ=255)
                                           gAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACtgt                                                                                      >  2:360909/1‑140 (MQ=255)
                                            aGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACtgtg                                                                                     <  2:518093/140‑1 (MQ=255)
                                               cGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTgtgtgt                                                                                  <  1:1337146/140‑1 (MQ=255)
                                                gggTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGtgtc                                                                                 >  2:2980907/1‑140 (MQ=255)
                                                     gATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCa                                                                            <  2:2417005/140‑1 (MQ=255)
                                                       tatCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCAtc                                                                          >  2:4350812/1‑140 (MQ=255)
                                                                tGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAATCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAAc                                                                 >  1:5272696/1‑140 (MQ=255)
                                                                tGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAAc                                                                 >  2:4856927/1‑140 (MQ=255)
                                                                   gTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaa                                                              >  2:3451362/1‑140 (MQ=255)
                                                                    tACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaa                                                             >  1:1478202/1‑140 (MQ=255)
                                                                     aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                            >  1:836865/1‑140 (MQ=255)
                                                                      ccagccagCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCTCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaaa                                                           >  2:4506706/1‑140 (MQ=255)
                                                                       cagccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAAt                                                          >  2:1747208/1‑140 (MQ=255)
                                                                       cagccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAAt                                                          >  1:555842/1‑140 (MQ=255)
                                                                        agccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGAGCGGAGCGGACAATATAGTGTGTGTCTGTCATATATTTGAACAAAAAATa                                                         >  2:1072256/1‑140 (MQ=255)
                                                                        agccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGAGCGAAGCGGCCAATATACTGCGCGTCTGTCATCCCTTTGAAAAAACAATa                                                         >  2:3487116/1‑140 (MQ=255)
                                                                            agcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGc                                                     >  1:3561550/1‑140 (MQ=255)
                                                                              cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCgg                                                   >  2:376472/1‑140 (MQ=255)
                                                                                 ccTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAg                                                <  1:1014860/140‑1 (MQ=255)
                                                                                 ccTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAg                                                <  1:3499362/140‑1 (MQ=255)
                                                                                 ccTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAg                                                <  2:1864859/140‑1 (MQ=255)
                                                                                    tGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAAGAAGGCGGGAGCAt                                             >  1:3099111/1‑140 (MQ=255)
                                                                                      ccGGCCGCGAGCCTGCAAATAGCGGCCGAGGTCGGCGATCAGCGAGCCACAATGAAGCTCGCCGGAAGGAGAGGGGGGGAAGCGGCCAATATACTGCGTGTCTGTCAGCTCTTTGAACAAACAATAAGGCGGGTGCAttt                                           <  2:1370764/140‑1 (MQ=255)
                                                                                          gcgcGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCg                                       <  2:4908015/140‑1 (MQ=255)
                                                                                           cgcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGc                                      <  2:2171232/140‑1 (MQ=255)
                                                                                           cgcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGc                                      >  2:3373861/1‑140 (MQ=255)
                                                                                           cgcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGc                                      <  2:2728612/140‑1 (MQ=255)
                                                                                             cgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCt                                    >  1:2586704/1‑140 (MQ=255)
                                                                                                   tGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTa                              <  2:3706956/140‑1 (MQ=255)
                                                                                                   tGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTa                              <  1:4180788/140‑1 (MQ=255)
                                                                                                          aGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGa                       >  1:3847379/1‑140 (MQ=255)
                                                                                                           gCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGAt                      >  2:2223872/1‑140 (MQ=255)
                                                                                                            cTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATg                     >  2:3326586/1‑140 (MQ=255)
                                                                                                               cggAGCGCGGCGATCAGAGAGCTAAAATGAATCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCTGGAGCATTCCCCGTCTGTGGGAAACTTGATGGaa                  <  2:1604791/138‑1 (MQ=255)
                                                                                                               ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                  <  1:3579508/140‑1 (MQ=255)
                                                                                                               ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                  <  2:624307/140‑1 (MQ=255)
                                                                                                               ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                  <  1:4012267/140‑1 (MQ=255)
                                                                                                               ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                  >  2:4580299/1‑140 (MQ=255)
                                                                                                               ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                  <  1:4299005/140‑1 (MQ=255)
                                                                                                               ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                  <  1:2821405/140‑1 (MQ=255)
                                                                                                               ccGAGCGAGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGCGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                  <  2:1035770/140‑1 (MQ=255)
                                                                                                                  aGCGGGGCGATCAGAAAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCATTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACgg               <  2:5341584/140‑1 (MQ=255)
                                                                                                                  aGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACgg               >  2:5048355/1‑140 (MQ=255)
                                                                                                                  aGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACgg               <  1:3432764/140‑1 (MQ=255)
                                                                                                                   gcgcGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCAAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGc              <  2:1456079/140‑1 (MQ=255)
                                                                                                                    cgcgGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCt             >  2:741066/1‑140 (MQ=255)
                                                                                                                      cggcgATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGCAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGt           >  2:574323/1‑140 (MQ=255)
                                                                                                                         cgATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAATCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACGAAAAATAAGGCGGGGGCATTTCCCGCCTGTGGTAAATGTGATGGAACGGcgggaag        >  2:5140217/1‑134 (MQ=255)
                                                                                                                               gagagCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGACCAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTagcca  <  1:4538966/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
TCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCA  >  NZ_CP025268/159720‑159986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑