Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,643,849	T→C	*166* (TAA→TAG)	rzpQ ←	DUF2514 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,643,849	0	T	C	100.0%	295.2 / NA	90	*166* (TAA→TAG)	rzpQ	DUF2514 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (64/26);  total (64/26)

TTTATTAATAAAACGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTGGTGAG  >  NZ_CP025268/1643713‑1643980                                                                                                                                         |                                                                                                                                    tttattAATAAAACGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTAc                                                                                                                                  >  2:186416/1‑140 (MQ=255)       aataaAACGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTCAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGa                                                                                                                            <  2:1022357/140‑1 (MQ=255)       aataaAACGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGa                                                                                                                            <  1:3853755/140‑1 (MQ=255)         taaAACGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATCCCGCGATGCAAATGTTCCGATTTAATATTACCCTACATTTGATg                                                                                                                          <  1:5172658/140‑1 (MQ=255)                tgtCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTa                                                                                                                   <  1:1908117/140‑1 (MQ=255)                 gtCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTAt                                                                                                                  >  2:5277513/1‑140 (MQ=255)                  tCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTAtc                                                                                                                 >  1:2188268/1‑140 (MQ=255)                       cTGATTAAGACCTTTTACCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACACTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGACGCTTTTTATTTCTTa                                                                                                            <  2:3287114/140‑1 (MQ=255)                        tGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTaa                                                                                                           <  2:1624051/140‑1 (MQ=255)                          aTTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAg                                                                                                         >  1:3930971/1‑140 (MQ=255)                          aTTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAg                                                                                                         >  1:3754063/1‑140 (MQ=255)                               gACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCa                                                                                                    >  1:4729470/1‑140 (MQ=255)                                 ccTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATa                                                                                                  <  1:757930/140‑1 (MQ=255)                                 ccTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATa                                                                                                  <  2:280085/140‑1 (MQ=255)                                    tttATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGAt                                                                                               >  2:1201923/1‑140 (MQ=255)                                    tttATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGAt                                                                                               >  1:59886/1‑140 (MQ=255)                                    tttATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGAt                                                                                               >  1:59354/1‑140 (MQ=255)                                    tttATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGAt                                                                                               >  2:609553/1‑140 (MQ=255)                                         cTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCGCTTAAAGATTCATAGATCTGtt                                                                                          >  1:3860407/1‑140 (MQ=255)                                           tACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGa                                                                                        >  2:4124407/1‑140 (MQ=255)                                            aCCCTTAAGATTTCAGGAATTTTGTCTCGTAGAAGAGACCTTTTTATTTAAATTTTACAATCCGCGACGTAAATGTTCAGACTTAATATTACCCTAAATATGATGCTTTTTATCACTTAAAGATTCATATACCTGTAGAc                                                                                       >  2:4405301/1‑140 (MQ=255)                                            aCCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAATTTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGAc                                                                                       >  1:3185985/1‑140 (MQ=255)                                            aCCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGAc                                                                                       >  1:575830/1‑140 (MQ=255)                                            aCCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGAc                                                                                       >  1:1543146/1‑140 (MQ=255)                                            aCCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGAc                                                                                       >  1:1663794/1‑140 (MQ=255)                                            aCCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGAc                                                                                       >  1:5344464/1‑140 (MQ=255)                                            aCCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGAc                                                                                       >  1:575892/1‑140 (MQ=255)                                             cccTTAAGATTTCAGGAATTTTGGCTCCTGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACa                                                                                      >  2:1487198/1‑140 (MQ=255)                                                ttAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGt                                                                                   <  1:49475/140‑1 (MQ=255)                                                ttAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGt                                                                                   <  1:4775133/140‑1 (MQ=255)                                                ttAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGt                                                                                   <  2:984309/140‑1 (MQ=255)                                                ttAAGATTTCAGGAATTTTCGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGt                                                                                   <  1:4145847/140‑1 (MQ=255)                                                  aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAACTTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCa                                                                                 >  1:4574194/1‑140 (MQ=255)                                                  aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACCTTCCGCGAGGTAAATGTTCCGATTTCATATTACCCTCCATTTGATGCTTTTTATCTCTTAAAGATTCAGCGATTCGTTGCCAAGGCa                                                                                 >  2:3635005/1‑140 (MQ=255)                                                  aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAATATTCATAGATCTGTTGACAAGTCa                                                                                 >  1:3769796/1‑140 (MQ=255)                                                  aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCa                                                                                 >  2:3206196/1‑140 (MQ=255)                                                  aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCa                                                                                 >  1:24686/1‑140 (MQ=255)                                                  aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCa                                                                                 >  2:3205867/1‑140 (MQ=255)                                                  aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCa                                                                                 >  1:1489108/1‑140 (MQ=255)                                                  aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTGACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCa                                                                                 >  1:1571186/1‑140 (MQ=255)                                                   aGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCAc                                                                                <  2:2829655/140‑1 (MQ=255)                                                   aGATTTCAGGAATTTTGGCTCATGGAAGAGTCATTTTTATTTAAATTTTACATGCCGCGATGTAAAGGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATTTCGTCAAGATTCATAGATCTGTTGACAAGGCAc                                                                                >  2:1305376/1‑140 (MQ=255)                                                        tCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTCAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCCGTTGACAAGTCACTCCTg                                                                           >  2:3817686/1‑140 (MQ=255)                                                        tCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGt                                                                                   <  2:4733997/132‑1 (MQ=255)                                                        tCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGt                                                                                   >  1:4733997/1‑132 (MQ=255)                                                          aGGAATTTTGGCTCATGGTAGAGTGCTTTTGATTGAAATTTTACATTCCGCGATCTAAATGTTCCGATTTGATATTAGCCTACATTTGATGCGTTTTATGTCTTAAATATTTATAGTTCTGTGTACAAGTCACTCCTGct                                                                         >  2:1538438/1‑139 (MQ=255)                                                                           gAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGc                                                        <  1:609553/140‑1 (MQ=255)                                                                                 tCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATTTAGCGTTCGTCAGCAAtttt                                                  >  1:3640635/1‑139 (MQ=255)                                                                                  ccTTTTTATTTAAATTTTACATTCCGCGAGGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCa                                                 >  1:2517597/1‑140 (MQ=255)                                                                                   cTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAg                                                >  2:2423253/1‑140 (MQ=255)                                                                                     ttttATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCa                                              <  1:5263147/140‑1 (MQ=255)                                                                                      tttatttaAATTTTACATTCCGCGATGTAAATGTTCCTCTTTAATATTACCCTACATTTGATTCTTTTTATCTGTTAAAGATTCTTAGATTTCTTGACAAGTCACTCCTTCGATGTAGCGTTCGTCAGCAATTGCAGCAt                                             >  2:3145573/1‑140 (MQ=255)                                                                                      tttatttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCAt                                             >  1:3382797/1‑140 (MQ=255)                                                                                           ttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGc                                        <  1:1095780/140‑1 (MQ=255)                                                                                           ttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGc                                        <  1:3621351/140‑1 (MQ=255)                                                                                           ttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGc                                        <  1:537953/140‑1 (MQ=255)                                                                                             aaaTTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTg                                      >  2:1990714/1‑140 (MQ=255)                                                                                                ttttACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGTCAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGc                                   <  2:2965151/140‑1 (MQ=255)                                                                                                          cgcgATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCa                         <  2:590381/140‑1 (MQ=255)                                                                                                          cgcgATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCa                         >  2:2979776/1‑140 (MQ=255)                                                                                                                gTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATAtct                   >  1:3005970/1‑140 (MQ=255)                                                                                                                gTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATAtct                   >  1:3006050/1‑140 (MQ=255)                                                                                                                 tAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATAtctc                  >  2:1033599/1‑140 (MQ=255)                                                                                                                  aaaTGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACCAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTcc                 <  1:1839633/140‑1 (MQ=255)                                                                                                                  aaaTGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTcc                 >  1:4180487/1‑140 (MQ=255)                                                                                                                  aaaTGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTcc                 >  1:4180725/1‑140 (MQ=255)                                                                                                                      gTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGc             <  1:2490184/140‑1 (MQ=255)                                                                                                                       ttCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGCTCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATACAGTTGAGCTTCTGCTCCAATATCTCCGAgga            >  2:3796483/1‑138 (MQ=255)                                                                                                                         ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGGTTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATGTCTCCGAGCATg          >  1:4570199/1‑140 (MQ=255)                                                                                                                         ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTTAGCTTCTGCTGCCATATCTCCGAGCATg          >  2:4749528/1‑140 (MQ=255)                                                                                                                         ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg          >  1:1909920/1‑140 (MQ=255)                                                                                                                         ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg          <  1:2884415/140‑1 (MQ=255)                                                                                                                         ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg          >  2:797358/1‑140 (MQ=255)                                                                                                                         ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg          >  1:690533/1‑140 (MQ=255)                                                                                                                         ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg          >  2:3933239/1‑140 (MQ=255)                                                                                                                         ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg          >  1:3512581/1‑140 (MQ=255)                                                                                                                         ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg          >  1:3118325/1‑140 (MQ=255)                                                                                                                         ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCAGAGCATg          >  2:66576/1‑140 (MQ=255)                                                                                                                         ccGATTGAGTATTACCCTACATTTGCTGCTTTTTATCTCTTAAAGGTTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTGAGCAATTTCAGCATAAGGCTGAGCTTCTGCTGCAATATCTCCGAGCATg          >  2:1147944/1‑140 (MQ=255)                                                                                                                         ccGAATTACTATTACCCTACATTTGATGCTTTTTATCTCTTATCTGTTCATAGTTTTGTTTACAAGTCACTCTTCTGATCTACCGTCGCTCCCCACTTTCCGCCTCAAGCTGAGCTTCTGGTGCAATCCCTCCcatcatc          >  2:556942/1‑139 (MQ=255)                                                                                                                           gATTTAATATTACCCTACATTTGTTGGTTTCTATCTCTTAAAGATTCATAGTTCTGTTGACAAGTCACTCCTGCGTTGTAGCGTTCGTCAACATTTTGAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGtt        >  1:3202051/1‑140 (MQ=255)                                                                                                                           gATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTATCGTTTGTCAGCAATTTCAGCATAAAGCTGAGATTCTGCTGCAATATCTCCCAGAATGtt        >  2:4003943/1‑140 (MQ=255)                                                                                                                           gATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGtt        >  1:4513198/1‑140 (MQ=255)                                                                                                                           gATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGtt        >  1:2351773/1‑140 (MQ=255)                                                                                                                           gATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGtt        >  1:250256/1‑140 (MQ=255)                                                                                                                           gATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGtt        >  1:4512919/1‑140 (MQ=255)                                                                                                                           gATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGtt        >  1:4371355/1‑140 (MQ=255)                                                                                                                           gATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGtt        >  1:2552535/1‑140 (MQ=255)                                                                                                                            aTTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTg       <  2:4494751/140‑1 (MQ=255)                                                                                                                                 atatTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTGGTGAg  <  1:3484895/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TTTATTAATAAAACGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTGGTGAG  >  NZ_CP025268/1643713‑1643980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑