BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,166	T→C	K109R (AAG→AGG)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,166	0	T	C	99.2%	780.5 / NA	239	K109R (AAG→AGG)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (92/145); minor base A (0/2); total (92/147)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGC  >  NZ_CP025268/1660029‑1660298

taAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTa                                                                                                                                       >  1:3964693/1‑140 (MQ=255)

  aaGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACg                                                                                                                                     >  1:1939342/1‑140 (MQ=255)

    gATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTg                                                                                                                                   <  2:4962974/140‑1 (MQ=255)

    gATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCGTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTg                                                                                                                                   <  1:4256876/140‑1 (MQ=255)

    gATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTAGATTTGCGTGGTGAATGGTTGACAAAAGATGAGATAGAACTCCTTTTGGCAGCTAACACTTCCTCTTTTCGTATTGATAAAATGGATTTATGTTCCTACGTg                                                                                                                                   <  2:5194784/140‑1 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                  >  2:1763504/1‑140 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                  >  2:3713784/1‑140 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                  <  1:11549/140‑1 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                  >  2:2156615/1‑140 (MQ=255)

       cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                               >  1:3497332/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c                                                                                                                             >  1:4824838/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c                                                                                                                             >  1:2908028/1‑140 (MQ=255)

          aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATCCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTCTGTTCCTACGTGCG‑c‑ccc                                                                                                                           <  1:3236444/140‑1 (MQ=255)

          aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑ccc                                                                                                                           <  2:1624997/140‑1 (MQ=255)

          aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑ccc                                                                                                                           <  2:4944502/140‑1 (MQ=255)

          aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑ccc                                                                                                                           <  1:3647783/140‑1 (MQ=255)

            aaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑cccca                                                                                                                         <  1:3757981/140‑3 (MQ=255)

               gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑ccccagcc                                                                                                                      <  1:5022189/140‑6 (MQ=255)

               gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑ccccagcc                                                                                                                      <  1:3826422/140‑6 (MQ=255)

               gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑ccccagcc                                                                                                                      <  1:2413518/140‑6 (MQ=255)

                ccGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGccc                                                                                                                     >  2:1336281/1‑140 (MQ=255)

                      tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACCCTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGcaaca                                                                                                               <  2:3887128/140‑1 (MQ=255)

                          cacaAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGa                                                                                                           >  2:3335910/1‑140 (MQ=255)

                           acaAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGAt                                                                                                          <  1:4457387/140‑1 (MQ=255)

                                      cGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGc                                                                                               <  1:2237489/140‑1 (MQ=255)

                                      cGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGc                                                                                               <  2:1187194/140‑1 (MQ=255)

                                          tatTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAg                                                                                           <  1:2638486/140‑1 (MQ=255)

                                               gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                      >  2:1271664/1‑140 (MQ=255)

                                               gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                      >  1:4336084/1‑140 (MQ=255)

                                                cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                     >  1:4507921/1‑140 (MQ=255)

                                                cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                     >  1:1892912/1‑140 (MQ=255)

                                                   tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                  <  2:4171440/140‑1 (MQ=255)

                                                    gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                 >  1:2148974/1‑140 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                >  1:834100/1‑140 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑C‑CCCGAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                >  2:4567217/1‑140 (MQ=255)

                                                       aaTGGTTTACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                              >  2:2506388/1‑140 (MQ=255)

                                                           gTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCg                                                                          >  1:3732428/1‑140 (MQ=255)

                                                             ttaCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAAGGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                        <  2:4470573/138‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGCATACCTTTTGTCAGCTGACCCTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGAAACAATGATCAACATGCCGCAAAGCCCAATCAACGAACCCGTc                                                                        <  2:560459/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑GGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                        <  1:1354844/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                        <  2:3667471/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                        <  2:3732428/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                        <  2:3497332/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                        <  1:1672630/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                        <  1:1454872/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                        <  2:5026317/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                        <  1:2865788/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCACTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCACCGAACCCGTc                                                                        <  2:626005/140‑1 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                      >  2:5214295/1‑140 (MQ=255)

                                                                    aGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCAt                                                                 <  2:4761391/140‑1 (MQ=255)

                                                                      aTGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaa                                                               >  1:2629361/1‑140 (MQ=255)

                                                                          aaTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                        <  2:200521/127‑1 (MQ=255)

                                                                           aTAGACTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCCAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                          <  1:5134212/140‑1 (MQ=255)

                                                                           aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                          <  1:964952/140‑1 (MQ=255)

                                                                           aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                          <  1:3429365/140‑1 (MQ=255)

                                                                               aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGt                                                      >  1:1807632/1‑140 (MQ=255)

                                                                                  aCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                   <  1:1519574/140‑1 (MQ=255)

                                                                                  aCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                   <  2:917924/140‑1 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                <  1:5230712/140‑1 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                <  2:3247653/140‑1 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                               >  2:1165270/1‑140 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                               <  1:3001368/140‑1 (MQ=255)

                                                                                        tGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCa                                             >  2:1047279/1‑140 (MQ=255)

                                                                                         gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAt                                            >  1:902944/1‑140 (MQ=255)

                                                                                             gCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcac                                        >  2:1323146/1‑140 (MQ=255)

                                                                                              cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                       >  2:2387206/1‑140 (MQ=255)

                                                                                                 acacTTCCTCGTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                    <  2:1856733/140‑1 (MQ=255)

                                                                                                  cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgc                                   >  1:4851333/1‑140 (MQ=255)

                                                                                                  cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGAGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgc                                   <  2:3831175/140‑1 (MQ=255)

                                                                                                   acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                  <  2:225100/140‑1 (MQ=255)

                                                                                                    cTTCCTCGTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAATGCGCAATCAACGCACCCGTCCAGTCATAAAGAGACAGTTTGACGCCATCCACAACgcgc                                 >  2:2743110/1‑140 (MQ=255)

                                                                                                      tCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAg                               >  2:756179/1‑140 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGCGTCAGTTTCACGCCATCCACAACGCGCAGc                              <  2:291966/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                              <  1:5351493/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                              <  1:1694403/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                              <  1:2048660/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                              <  1:4032977/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                              <  2:1172614/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                              <  2:1468459/140‑1 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                             >  2:1490383/1‑140 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                              <  2:3562707/139‑1 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                              >  1:3562707/1‑139 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                    <  2:2690547/140‑1 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                    >  2:1880722/1‑140 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAAAAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                    <  1:664361/140‑1 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                   >  2:1637435/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                  >  1:1130693/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                  >  1:3582236/1‑140 (MQ=255)

                                                                                                                      aTAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg               <  1:1009966/140‑1 (MQ=255)

                                                                                                                      aTAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg               <  1:4116563/140‑1 (MQ=255)

                                                                                                                      aTAAAATGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg               <  1:2293743/140‑1 (MQ=255)

                                                                                                                        aaaaTGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa             <  2:2087973/140‑1 (MQ=255)

                                                                                                                         aaaTGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAg            <  1:4613629/140‑1 (MQ=255)

                                                                                                                          aaTGGATTTATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGGCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGa           >  2:4095957/1‑140 (MQ=255)

                                                                                                                                tttATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                          <  2:4998497/87‑1 (MQ=255)

                                                                                                                                tttATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                          >  1:4998497/1‑87 (MQ=255)

                                                                                                                                 ttATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAAc    >  1:3076820/1‑140 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg   <  2:5138328/140‑1 (MQ=255)

                                                                                                                                   aTGTTCCTACGTGCGCC‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGc  <  2:902087/140‑1 (MQ=255)

                                                                                                                                   aTGTTCCTACGTGCG‑CACCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGc  <  2:4021782/140‑1 (MQ=255)

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑CCC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGC  >  NZ_CP025268/1660029‑1660298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`