BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,696,010	A→G	Q447Q (CAA→CAG)	ydgA →	YdgA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,696,010	0	A	G	99.5%	723.5 / NA	217	Q447Q (CAA→CAG)	ydgA	YdgA family protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (68/147); total (68/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

TGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAA  >  NZ_CP025268/1695874‑1696144

tGGATGCGGAATTGACCATTCCGGTGGATATGGCAACTGAGTTTTTGACTCAGGTAGCGGAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAGAACTGGCGAAACAGTAAGGTGAAGGTGCATCCGCAATGGAGCAGAtt                                                                                                                                     >  2:3798917/1‑139 (MQ=255)

tGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAGGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATg                                                                                                                                     >  2:564676/1‑140 (MQ=255)

tGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATg                                                                                                                                     >  1:5043765/1‑140 (MQ=255)

tGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCAGCAGCAATGGGGCAGATg                                                                                                                                     >  1:648123/1‑140 (MQ=255)

   aTGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTc                                                                                                                                  <  2:5257360/140‑1 (MQ=255)

          aCTGACCATTCCGGTGGATATGGCAACTGAGTTTATCACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGa                                                                                                                           <  1:5287032/140‑1 (MQ=255)

           cTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCGATGGGGCAGATGTTCCGTCTGac                                                                                                                          <  1:3204593/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGGAGCTGGAAGGTTATCAGGAAAATCAAGCGAAAAAACTGGCGCAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  1:3196443/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  1:389128/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  2:4498886/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  1:1493876/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  2:1331915/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  1:2448432/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  2:1441971/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  1:103454/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  2:241061/140‑1 (MQ=255)

              aCCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGaccac                                                                                                                       >  1:1067211/1‑140 (MQ=255)

                cATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCt                                                                                                                     >  1:4297718/1‑140 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                   <  2:2899646/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                   <  2:5106681/140‑1 (MQ=255)

                  ttCCGGTGGAGATGGGAATTGAGTTTATGCCTCAGGTAGCGAAGCTGGAAGGTTAGCAGGGAGATCAAGCGGAAAACCTGGCGAAACAGCAAGTTGAAGGTGCAGCAGCAATGGTGCAGATGTTCCGTTTGACCACCTTg                                                                                                                   <  2:4412046/140‑1 (MQ=255)

                   tCCGGTGGGTATGGCAACTGAGTTTATGACTCAGGTAGCGAAGTTGGAAGGTTATCAGGAAGAGCGAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGc                                                                                                                  <  2:2319849/140‑1 (MQ=255)

                   tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGc                                                                                                                  >  1:2126314/1‑140 (MQ=255)

                    ccGGTGGATATGCCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCa                                                                                                                 <  2:1124563/140‑1 (MQ=255)

                       gTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGa                                                                                                              <  1:3316991/140‑1 (MQ=255)

                       gTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGa                                                                                                              <  1:4924812/140‑1 (MQ=255)

                        tGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGAc                                                                                                             <  1:3313908/140‑1 (MQ=255)

                        tGGATATGGCAACTGAGTGTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGAc                                                                                                             <  2:3084818/140‑1 (MQ=255)

                          gATATGATAACTGAGTTGAGGACTCAGGGAGCGAAGCCAGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGAGAAACAGCAAGTTGATGTTGCCTCAGCAATGGGGCGGATGTTCCGTCCGACCATCTTGCTGGaaaa                                                                                                           <  2:3962412/140‑1 (MQ=255)

                           atatGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAGAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCCCCTTGTAGGACAAt                                                                                                          <  2:3205249/140‑1 (MQ=255)

                           atatGGCAACGGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAACAAACTGGCGAAACAGCAAGTTGACGGTGCATCAGCAAGGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAAt                                                                                                          <  1:825820/140‑1 (MQ=255)

                            tatGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATa                                                                                                         <  2:2106414/140‑1 (MQ=255)

                              tGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAACCAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATAcc                                                                                                       <  1:1135146/140‑1 (MQ=255)

                                gCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCAt                                                                                                     <  2:4863313/140‑1 (MQ=255)

                                gCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCAt                                                                                                     <  1:3855181/140‑1 (MQ=255)

                                 cAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATc                                                                                                    >  2:2722996/1‑140 (MQ=255)

                                    cTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacc                                                                                                 >  2:3740202/1‑140 (MQ=255)

                                    cTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacc                                                                                                 >  2:344683/1‑140 (MQ=255)

                                     tGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                                <  2:5043644/140‑1 (MQ=255)

                                     tGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                                <  1:2361109/140‑1 (MQ=255)

                                     tGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                                >  1:2877498/1‑140 (MQ=255)

                                     tGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                                <  1:5098279/140‑1 (MQ=255)

                                      gAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                                                                               <  2:5072616/140‑1 (MQ=255)

                                        gTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCAccacca                                                                                             <  1:2563488/140‑1 (MQ=255)

                                        gTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCAccacca                                                                                             <  1:887866/140‑1 (MQ=255)

                                        ctttACGACTAAGGTAGCGACCCCGGAAGCTTATCCGGAAAATCAAGCGAAAAAACTGGCGAACCAGCAACTTGAAGGTGCCTCAGCAATCGCCCAGATATTCAGTCGGACCACCTTGCAGAACAATCCCATCCccagca                                                                                             <  2:92514/139‑1 (MQ=255)

                                          ttATGAGTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGTTGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                           <  2:2512409/140‑1 (MQ=255)

                                          ttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                           <  1:4773218/140‑1 (MQ=255)

                                          ttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGACCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                           <  1:3409839/140‑1 (MQ=255)

                                          atATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                           <  1:4727249/139‑1 (MQ=255)

                                               acgcAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCCGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCa                                                                                      >  1:4656752/4‑140 (MQ=255)

                                                cTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCaa                                                                                     <  2:252967/140‑1 (MQ=255)

                                                cTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCaa                                                                                     <  2:2929571/140‑1 (MQ=255)

                                                cTCAGGTAGCCAAGCTGGAGGGTTATCATGAAGATCAAGCGAAAAAACTGGCCAAACAGCCAGTTGCAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCaa                                                                                     <  2:2928933/140‑1 (MQ=255)

                                                   aGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAatat                                                                                  >  1:4685753/1‑140 (MQ=255)

                                                   aGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAatat                                                                                  >  1:4860450/1‑140 (MQ=255)

                                                   aGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAatat                                                                                  >  1:4053980/1‑140 (MQ=255)

                                                   aGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAatat                                                                                  >  2:1902477/1‑140 (MQ=255)

                                                   aGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGGTGAAGGTGCATCAGAAATGGGGCAGATGTACAGACTGACAACCTTGCAGGAAAATACCAGCACCACCCGCCTGCAatat                                                                                  >  2:276255/1‑140 (MQ=255)

                                                      tAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCt                                                                               <  1:2799338/140‑1 (MQ=255)

                                                      tAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCt                                                                               <  1:3010680/140‑1 (MQ=255)

                                                      tAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCt                                                                               <  2:639509/140‑1 (MQ=255)

                                                       aGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGCAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACTAACTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTa                                                                              <  2:2953898/140‑1 (MQ=255)

                                                           aaGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACgg                                                                          <  1:202074/140‑1 (MQ=255)

                                                                ggAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGa                                                                     <  1:1260158/140‑1 (MQ=255)

                                                                ggAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGa                                                                     >  1:1025740/1‑140 (MQ=255)

                                                                ggAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGCTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGa                                                                     <  2:2341258/140‑1 (MQ=255)

                                                                 gAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGAt                                                                    <  2:3564262/140‑1 (MQ=255)

                                                                 gAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGAt                                                                    <  2:1068146/140‑1 (MQ=255)

                                                                 gAAGGTTATCAGGAAGATAAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCACTGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCCACACCAGCCTGCAATATGCTAACGGTCAGAt                                                                    <  2:4154662/140‑1 (MQ=255)

                                                                 gAAGGTTATCAAGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGAt                                                                    <  2:4824203/140‑1 (MQ=255)

                                                                         tCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTaa                                                            <  1:4533437/140‑1 (MQ=255)

                                                                         tCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTaa                                                            <  1:4117899/140‑1 (MQ=255)

                                                                               aGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGc                                                      <  2:536923/140‑1 (MQ=255)

                                                                                gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGAt                                                                    <  1:1983298/125‑1 (MQ=255)

                                                                                gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCa                                                     >  1:1943449/1‑140 (MQ=255)

                                                                                gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCa                                                     >  1:2186216/1‑140 (MQ=255)

                                                                                gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCa                                                     >  2:800989/1‑140 (MQ=255)

                                                                                    aaGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGaaa                                                 <  2:4259404/140‑1 (MQ=255)

                                                                                     aGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGaaaa                                                >  1:4183160/1‑140 (MQ=255)

                                                                                      gCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAAt                                               <  1:4275612/140‑1 (MQ=255)

                                                                                              aCTGCCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACATTGAAGGACAATAAAATCCACACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTag                                       <  2:4612758/140‑1 (MQ=255)

                                                                                                 ggCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTagaag                                    >  2:1380326/1‑140 (MQ=255)

                                                                                                    gAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGAtt                                 <  2:255814/140‑1 (MQ=255)

                                                                                                        cAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTttgt                             <  1:1575516/140‑1 (MQ=255)

                                                                                                           cAATTTGACGGTGCATCCGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCCGCCTGCAATATTCTAACGGTCAGATAACGTTAAATGGGCATAAAATGTCGTTAGAAGATTTTGTTgg                          <  2:3011793/140‑1 (MQ=255)

                                                                                                           aaaGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCACATAACCTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTgg                          <  1:965010/139‑1 (MQ=255)

                                                                                                              gTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCAt                       <  1:4495745/140‑1 (MQ=255)

                                                                                                                   aGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGCTTTTGTTGGCATGTTTg                  >  1:700455/1‑140 (MQ=255)

                                                                                                                        cATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATGACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATg             >  1:4790224/1‑140 (MQ=255)

                                                                                                                         aTCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCCGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAACGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGc            <  2:2744876/140‑1 (MQ=255)

                                                                                                                         aTCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGc            <  2:1985386/140‑1 (MQ=255)

                                                                                                                         aTCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGc            <  2:1614684/140‑1 (MQ=255)

                                                                                                                         aTCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGc            <  1:3263035/140‑1 (MQ=255)

                                                                                                                         aTCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGc            <  1:3740202/140‑1 (MQ=255)

                                                                                                                          tCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGcc           <  1:3923729/140‑1 (MQ=255)

                                                                                                                          tCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGcc           <  1:3283325/140‑1 (MQ=255)

                                                                                                                             ccAATGGGGCAGATGTTCCGTCTGAACACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAACTAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGc        <  2:2973337/139‑1 (MQ=255)

                                                                                                                                aTGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGctct     >  1:5011479/1‑140 (MQ=255)

                                                                                                                                   gggCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTaa  >  1:435986/1‑140 (MQ=255)

TGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAA  >  NZ_CP025268/1695874‑1696144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`