BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,738,522	A→G	1539 (TAA→TAG)	lhr →	ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,738,522	0	A	G	99.3%	915.0 / NA	274	1539 (TAA→TAG)	lhr	ATP‑dependent helicase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (128/144); total (129/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

CCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGATACATTTCG  >  NZ_CP025268/1738386‑1738657

ccGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGCAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATagaga                                                                                                                                      <  2:4623145/140‑1 (MQ=255)

 cGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATagagaa                                                                                                                                     <  1:4553871/140‑1 (MQ=255)

 cGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATagagaa                                                                                                                                     >  2:4887803/1‑140 (MQ=255)

      tGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGAc                                                                                                                                <  1:1176582/140‑1 (MQ=255)

        cGTCGCGAACCACGGCTGCGTTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTg                                                                                                                              >  2:5286527/1‑140 (MQ=255)

        cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTg                                                                                                                              >  1:3954834/1‑140 (MQ=255)

        cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTg                                                                                                                              >  1:2978325/1‑140 (MQ=255)

        cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTg                                                                                                                              >  1:2675025/1‑140 (MQ=255)

        cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTg                                                                                                                              >  2:4294447/1‑140 (MQ=255)

        cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTg                                                                                                                              >  1:1857487/1‑140 (MQ=255)

        cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGAGTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTg                                                                                                                              >  2:1463217/1‑140 (MQ=255)

        cGTCCCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATGTTCAAGTTCGCCACAAGGGCTGGCTTGGGGATAGAGAAAGGACTg                                                                                                                              >  2:538685/1‑140 (MQ=255)

          tCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGAc                                                                                                                            >  2:199409/1‑140 (MQ=255)

           cgcgAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACg                                                                                                                           >  2:4968784/1‑140 (MQ=255)

              gAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGAt                                                                                                                        <  1:3999875/140‑1 (MQ=255)

                aCCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGc                                                                                                                      >  2:2731823/1‑140 (MQ=255)

                    cGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGt                                                                                                                  >  2:3609366/1‑140 (MQ=255)

                       cTGCGCTTTACGCTAACAGAAGTGCATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCg                                                                                                               <  1:3411092/140‑1 (MQ=255)

                         gcgcTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCa                                                                                                             <  2:1756106/140‑1 (MQ=255)

                         gcgcTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCa                                                                                                             <  1:2838961/140‑1 (MQ=255)

                          cgcTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCAt                                                                                                            >  1:1809211/1‑140 (MQ=255)

                          cgcTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCAt                                                                                                            >  1:111434/1‑140 (MQ=255)

                            cTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGGGAGGCGGGATTTTCAAGTTCGCCAGAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATcc                                                                                                          >  2:1926617/1‑140 (MQ=255)

                              ttACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTGAAGTTTGCCACGAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGt                                                                                                        <  2:3825583/140‑1 (MQ=255)

                              ttACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGt                                                                                                        <  1:1016267/140‑1 (MQ=255)

                               tACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGAGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTc                                                                                                       >  1:623344/1‑140 (MQ=255)

                                 cGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAg                                                                                                     <  1:777145/140‑1 (MQ=255)

                                 cGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAg                                                                                                     >  1:5312421/1‑140 (MQ=255)

                                     aaCAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTAtt                                                                                                 <  1:841848/140‑1 (MQ=255)

                                      aCAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCTCGTTCGCATCCGTCAGTATTg                                                                                                >  2:3988808/1‑140 (MQ=255)

                                      aCAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTg                                                                                                <  2:363187/140‑1 (MQ=255)

                                       cAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGTTGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGc                                                                                               >  2:3413487/1‑140 (MQ=255)

                                          aaGTGAATGATCTCCCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGCGATAGAGAAAGGACTGACGGCTGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            <  1:1556116/140‑1 (MQ=255)

                                          aaGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            <  2:3892231/140‑1 (MQ=255)

                                          aaGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            <  1:4846438/140‑1 (MQ=255)

                                          aaGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            <  1:4686940/140‑1 (MQ=255)

                                          aaGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            <  2:1962917/140‑1 (MQ=255)

                                          aaGTGAATGATCTACCGGGCCGCCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            <  2:2545355/140‑1 (MQ=255)

                                           aGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGa                                                                                           >  2:3765571/1‑140 (MQ=255)

                                           aGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGa                                                                                           >  1:2221047/1‑140 (MQ=255)

                                            gTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGAc                                                                                          <  1:3511698/140‑1 (MQ=255)

                                                aggATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCGGCGTGAGGCGGGATGTTCAAGTTCGCCACAAGGGCTGGATGGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCGGTATTGCAGGACGGat                                                                                      >  1:4270604/3‑140 (MQ=255)

                                                      tACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTcc                                                                                >  1:5142163/1‑140 (MQ=255)

                                                         cGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCcgcg                                                                             >  1:3230117/1‑140 (MQ=255)

                                                           gTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTc                                                                           <  1:729476/140‑1 (MQ=255)

                                                           gTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTc                                                                           >  1:33100/1‑140 (MQ=255)

                                                              cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCTCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                                        >  2:798876/1‑140 (MQ=255)

                                                              cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                                        <  2:1423045/140‑1 (MQ=255)

                                                              cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                                        >  1:4656708/1‑140 (MQ=255)

                                                              cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                                        >  2:1168129/1‑140 (MQ=255)

                                                              cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                                        <  1:3657982/140‑1 (MQ=255)

                                                              cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                                        >  2:626760/1‑140 (MQ=255)

                                                              cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                                        <  2:3230117/140‑1 (MQ=255)

                                                              cGGCAAACCCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGCGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTcgcg                                                                        >  2:1537561/1‑137 (MQ=255)

                                                               ggCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGc                                                                       <  2:2593183/140‑1 (MQ=255)

                                                                gCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGCGAAAGGACTGACGGATGCCCGTTCGCATCAGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGct                                                                      <  1:1677679/140‑1 (MQ=255)

                                                                   aaCGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCtt                                                                   <  1:1932146/140‑1 (MQ=255)

                                                                   aaCGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCtt                                                                   <  1:2480903/140‑1 (MQ=255)

                                                                   aaCGCCGATGTTTACGCTGCTGCGTAAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCtt                                                                   <  1:4871758/140‑1 (MQ=255)

                                                                     cgccgcTGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCa                                                                 <  1:2182759/140‑1 (MQ=255)

                                                                      gCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGt                                                                                                        >  1:354795/1‑100 (MQ=255)

                                                                       ccGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGa                                                               <  2:4407704/140‑1 (MQ=255)

                                                                        cGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGAc                                                              <  2:778139/140‑1 (MQ=255)

                                                                                aCGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTAttt                                                      >  1:944455/1‑140 (MQ=255)

                                                                                    tgctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCg                                                  >  2:2753790/1‑140 (MQ=255)

                                                                                     gctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCgg                                                 <  2:843543/140‑1 (MQ=255)

                                                                                      ctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGc                                                >  2:18730/1‑140 (MQ=255)

                                                                                      ctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGc                                                <  2:3662678/140‑1 (MQ=255)

                                                                                      ctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGc                                                >  1:4473874/1‑140 (MQ=255)

                                                                                       tgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCa                                               >  2:2916543/1‑140 (MQ=255)

                                                                                       tgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCa                                               >  1:4602727/1‑140 (MQ=255)

                                                                                        gtgTGAGGCGGGATTTTCAATTTCCCCAGAAGGGCTGGATTGGGGATCGAGCAAGGACTGACGGATGCCCGTTCGCATCCGTCACTATTGCAACACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAg                                              <  2:2050238/138‑1 (MQ=255)

                                                                                              ggCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTATCGGCAGTTTCtt                                        >  2:4759781/1‑140 (MQ=255)

                                                                                              ggCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCtt                                        <  2:4237946/140‑1 (MQ=255)

                                                                                                cGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTg                                      >  1:969399/1‑140 (MQ=255)

                                                                                                cGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTg                                      >  1:4938297/1‑140 (MQ=255)

                                                                                                   gATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATc                                   <  1:4514571/140‑1 (MQ=255)

                                                                                                   gATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCGCTTCAGACTTTTATTTAGCGGCAGTTTCTTTGATc                                   >  1:524927/1‑140 (MQ=255)

                                                                                                      tttCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTTCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAgc                                <  2:955730/140‑1 (MQ=255)

                                                                                                           aGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGctgctg                           <  1:2751023/140‑1 (MQ=255)

                                                                                                             ttCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCa                         <  1:4844646/140‑1 (MQ=255)

                                                                                                                  cacaAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCg                    >  2:4039057/1‑140 (MQ=255)

                                                                                                                   ccaAGTGCTTCATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTAGCATCCGTCAGTATTGCAGGACTGCTTATTCCGCGTCCGGCTCGTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc                   <  2:1381352/139‑1 (MQ=255)

                                                                                                                   acaAGGGCTGGGTTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGGCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc                   <  2:4906665/140‑1 (MQ=255)

                                                                                                                   acaAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTCTTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc                   <  2:4250746/140‑1 (MQ=255)

                                                                                                                   acaAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc                   <  1:2017407/140‑1 (MQ=255)

                                                                                                                   acaAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc                   <  2:169547/140‑1 (MQ=255)

                                                                                                                   acaAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc                   <  1:4170949/140‑1 (MQ=255)

                                                                                                                   acaAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc                   <  1:4526114/140‑1 (MQ=255)

                                                                                                                   acaAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc                   <  2:5136322/140‑1 (MQ=255)

                                                                                                                     acgggCTCGATTGGGGAAAAAGAAAGGACTGACGGATGCCAGTTCGCAGGCGTCAGCATGGCAGGACGGATTATGCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCa                 <  2:4049388/138‑1 (MQ=255)

                                                                                                                       gggCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACg               <  1:1329956/140‑1 (MQ=255)

                                                                                                                       gggCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACg               <  1:4322217/140‑1 (MQ=255)

                                                                                                                          cTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTg            >  1:905751/1‑140 (MQ=255)

                                                                                                                           tGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGa           <  1:1395468/140‑1 (MQ=255)

                                                                                                                            ggATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTTAt          >  2:245613/1‑140 (MQ=255)

                                                                                                                            ggATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGAt          >  1:513626/1‑140 (MQ=255)

                                                                                                                               ttGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGATACa       >  1:3273193/1‑140 (MQ=255)

                                                                                                                                  gggATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGATACAttt    >  1:496826/1‑140 (MQ=255)

                                                                                                                                  gggATAGAGAAAGGACTGACGGATGCCCGTTCGCATACGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGGATTTAGCGGCAGGTTCTTTGAGCAGCTGCTGCAGTTCGCCACGCGGATGCAttt    >  2:2208817/1‑140 (MQ=255)

CCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGATACATTTCG  >  NZ_CP025268/1738386‑1738657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`