Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,797,084 T→C *155* (TAA→TAG)  nlpC ← NlpC/P60 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,797,0840TC99.1% 354.9 / ‑6.1 110*155* (TAA→TAG) nlpCNlpC/P60 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (66/43);  minor base A (1/0);  total (67/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 110 total aligned reads displayed.

TTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGT  >  NZ_CP025268/1796949‑1797219
                                                                                                                                       |                                                                                                                                       
ttttCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGa                                                                                                                                     <  1:2912005/140‑1 (MQ=255)
 tttCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGAt                                                                                                                                    >  2:2631206/1‑140 (MQ=255)
         gTCCTTTTTTTAGATTATCCTGATTATAAACGGATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGTCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCg                                                                                                                            >  2:4518829/1‑140 (MQ=255)
         gTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCg                                                                                                                            >  2:5274656/1‑140 (MQ=255)
         gTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCg                                                                                                                            >  2:563300/1‑140 (MQ=255)
                          tCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTCCTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCCGCCATAAGtttatg                                                                                                           >  1:2297640/1‑137 (MQ=255)
                          tCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTc                                                                                                           <  1:2729519/140‑1 (MQ=255)
                               aTTATAACCCAATACGCCATTTACGGGATTTTTGTCTGCCAAATACTACTGTATTCAGGAGTAAATGGACGGCGCTGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCa                                                                                                      <  1:362377/140‑1 (MQ=255)
                               aTTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCa                                                                                                      <  1:1396677/140‑1 (MQ=255)
                               aTTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCACCAGTTTTTCttaca                                                                                                      >  1:1143183/1‑136 (MQ=255)
                               aTTATAAACGAATAATCCATTTACGGAATTTGTGTCTGCAAAATAGTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCa                                                                                                      >  2:4076015/1‑140 (MQ=255)
                                ttATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCaaa                                                                                                     >  2:3699804/1‑140 (MQ=255)
                                ttATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCaa                                                                                                     <  1:3128815/140‑1 (MQ=255)
                                 tataAACGAATCATCCATTTACGGAATTTTTGTATGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCCACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAAt                                                                                                    <  2:1074394/140‑1 (MQ=255)
                                 tataAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAAt                                                                                                    <  1:2216606/140‑1 (MQ=255)
                                   taAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAg                                                                                                  <  1:3549136/140‑1 (MQ=255)
                                    aaaCGAATAAGCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGGCCATTCTTCGACACACGAGATACGTCACGCCTGCCAGAAATTGTTCTGCCAAAAGa                                                                                                 >  2:3249743/1‑140 (MQ=255)
                                      aCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACa                                                                                               <  1:5309541/140‑1 (MQ=255)
                                         aataatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTTGACATACTAGATTCGTCTCGCCCCCCATAAATTTTTCTGCCTTTTGACATTa                                                                                            >  1:2629867/1‑140 (MQ=255)
                                         aataatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCCGCCAGAAATTTTTCTGCCAATTGACATTa                                                                                            >  1:2602507/1‑140 (MQ=255)
                                         aataatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTAGACATACTAGATTCGTCTCGCCTGACAGAAATTTTTCTGGCAATAGACAttt                                                                                            >  2:1999643/1‑139 (MQ=255)
                                          ataatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTAt                                                                                           <  2:5009382/140‑1 (MQ=255)
                                          ataatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTAt                                                                                           <  1:1831427/140‑1 (MQ=255)
                                          ataatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTAt                                                                                           <  1:1831618/140‑1 (MQ=255)
                                           taatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCATGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATc                                                                                          <  1:5316891/140‑1 (MQ=255)
                                           taatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATc                                                                                          <  1:1227912/140‑1 (MQ=255)
                                           taatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATc                                                                                          >  2:1507162/1‑140 (MQ=255)
                                               ccATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGt                                                                                      <  1:4655331/140‑1 (MQ=255)
                                                cATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTg                                                                                     <  1:3030568/140‑1 (MQ=255)
                                                  tttACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAg                                                                                   >  2:2465041/1‑140 (MQ=255)
                                                   ttACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGGCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAgg                                                                                  >  2:300972/1‑140 (MQ=255)
                                                    tACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGa                                                                                 <  1:3520506/140‑1 (MQ=255)
                                                     aCGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGaa                                                                                <  1:110360/140‑1 (MQ=255)
                                                        gAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGc                                                                             >  2:5060879/1‑140 (MQ=255)
                                                         aaTTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCa                                                                            <  1:4324101/140‑1 (MQ=255)
                                                                gTCTGCACAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTCTCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGGGAGGAACGCATCACTcc                                                                     <  1:2806910/140‑1 (MQ=255)
                                                                gTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTcc                                                                     <  1:1380017/140‑1 (MQ=255)
                                                                gTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTcc                                                                     <  1:1730746/140‑1 (MQ=255)
                                                                 tCTGCAAAATACTACTGTATTCAGGAGTAAATGGATGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTccc                                                                    >  2:3239076/1‑140 (MQ=255)
                                                                 ccTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATATATCATCGCCGTGCATATTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTccc                                                                    <  1:301181/139‑1 (MQ=255)
                                                                  cTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCt                                                                   <  2:3781189/140‑1 (MQ=255)
                                                                          tactacTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTa                                                           <  1:939006/140‑1 (MQ=255)
                                                                          tactacTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTa                                                           <  1:77183/140‑1 (MQ=255)
                                                                               ctGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGc                                                      >  2:2770463/1‑140 (MQ=255)
                                                                               ctGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGc                                                      >  2:2770249/1‑140 (MQ=255)
                                                                               ctGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGc                                                      >  2:2747148/1‑140 (MQ=255)
                                                                                 gTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGt                                                    <  1:3299460/140‑1 (MQ=255)
                                                                                 gTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGt                                                    <  1:3299485/140‑1 (MQ=255)
                                                                                  tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTg                                                   >  2:4243272/1‑140 (MQ=255)
                                                                                  tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGAGTCGTCTCGCCTGCCAGAGATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATAACGCCCTTGCTGGTAGAGGCgag                                                   >  2:4467460/1‑138 (MQ=255)
                                                                                    ttCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGa                                                 <  1:898288/140‑1 (MQ=255)
                                                                                    ttCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGa                                                 <  1:1275448/140‑1 (MQ=255)
                                                                                    ttCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCACTTGCTGGTAGAGGCGTGGa                                                 >  2:3248393/1‑140 (MQ=255)
                                                                                    ttCAGGAGTAAAGGGACGGCAATGTATCATCGCTGTGAATTTTTCGACATACTAAATTCGACTCGCCTGCCAGAAATTTTTCTGCCATGTGACATTATCAAGTGTGGAACGCATCTCTCCCTTGCTGGTAGGTGCGTGGa                                                 >  2:428332/1‑140 (MQ=255)
                                                                                       aGGAGTAAATGGTCGGCGATGTATCATCGCCGTACATTGTTCGACATACTAGATGCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAAGGAGGAACGCATCACTCCCTTGCTGGTAGAGTCGTGGATaa                                              >  2:3556149/1‑140 (MQ=255)
                                                                                       aGGAGTAAATGGACGGCGATGTATCATCGCCGTGCATTTTTCGACCTACTCGATTCGTCTCCCCGGCCAGAAACTTTTCTGCCAATAGACAGTATCAAGTGAAGAACGCATCTCTCCCCTGCCGGTAGCGGCGAGGATaa                                              >  2:644444/1‑140 (MQ=255)
                                                                                       aGGAGTAAATGGACGGCGATGTATCATCGCAGTCCATTTTTCGTCATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATaa                                              >  2:4895742/1‑140 (MQ=255)
                                                                                         gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTGCCTTGCTGGTAGAGGCGTGGATAAAt                                            <  1:4533159/140‑1 (MQ=255)
                                                                                         gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGAAATACTAGAGTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAt                                            >  2:246811/1‑140 (MQ=255)
                                                                                            tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGATGCGTGGATAAATTgg                                         >  2:2967594/1‑140 (MQ=255)
                                                                                            tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTgg                                         >  2:1807160/1‑140 (MQ=255)
                                                                                            tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTgg                                         >  2:1118895/1‑140 (MQ=255)
                                                                                              aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATc                                                                                          >  2:3373218/1‑89 (MQ=255)
                                                                                              aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATc                                                                                          <  1:3373218/89‑1 (MQ=255)
                                                                                              aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTTgtt                                       >  2:1489117/1‑140 (MQ=255)
                                                                                              aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgtt                                       >  2:728277/1‑140 (MQ=255)
                                                                                              aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgtt                                       >  2:532598/1‑140 (MQ=255)
                                                                                              aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgtt                                       >  2:572043/1‑140 (MQ=255)
                                                                                              aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgtt                                       >  2:1185930/1‑140 (MQ=255)
                                                                                              aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgtt                                       >  2:2367595/1‑140 (MQ=255)
                                                                                              aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTATCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGAGAGGAACTCATTACTCCCTTGCTGGTAAAGGCGTGGATAAATTGgtt                                       >  2:3240445/1‑140 (MQ=255)
                                                                                              aaTGGACGGCGATGTATCATCGCCGGCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGACCGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATCGgtt                                       >  2:2290702/1‑140 (MQ=255)
                                                                                                     ggCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTa                                <  1:2829678/140‑1 (MQ=255)
                                                                                                      gCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTAt                               >  2:228630/1‑140 (MQ=255)
                                                                                                      gCGATGTATCATCGCCGGCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTAt                               >  2:2617703/1‑140 (MQ=255)
                                                                                                       cGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATc                              <  1:3763305/140‑1 (MQ=255)
                                                                                                          tGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTa                           >  2:2144558/1‑140 (MQ=255)
                                                                                                            tATCATCGCCGTCCCTTTTTCGACATACTATATTCGTCTCGCCTGCCAGAAATCTTTCTGCCAATAGACATTATCAAGTGAGGAACGCAGCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATAGTaaa                         >  2:491753/1‑140 (MQ=255)
                                                                                                            tATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTTCTGGTAGAGGCGTGGATAAATTGGTGGTTTGTATCGTaaa                         >  2:45750/1‑140 (MQ=255)
                                                                                                            tATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaaa                         <  1:1226949/140‑1 (MQ=255)
                                                                                                               catcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCGTGCTGTTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATAc                      >  2:1724246/1‑140 (MQ=255)
                                                                                                                 tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGGAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt                    >  2:3288806/1‑140 (MQ=255)
                                                                                                                 tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt                    >  2:69547/1‑140 (MQ=255)
                                                                                                                 tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt                    >  2:5256036/1‑140 (MQ=255)
                                                                                                                 tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt                    >  2:5312677/1‑140 (MQ=255)
                                                                                                                    ccGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGCAATTTTTCTCCCAATAGACATTATCAAGTGAGAAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATACAATGTTTGTCGGTACCCGAAATAACTACa                 >  1:2377955/1‑140 (MQ=255)
                                                                                                                      gtgcATTTGTGGACATACTAGATTCGTCTCGCCGGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCGGGTAGAGGCGTGGATAAATTGGTTGTTGGGATCGTAAATAGCTACATg               >  2:1155212/4‑140 (MQ=255)
                                                                                                                      gTCCCTTTTTCGACATAATAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCAACTAGACATTACTAAGTGAGTAACGCATCACTACCTTGCTGGTATACGCGTGGATAACGTCGTTGTGACTGTCGCAAATACCCACCTg               >  2:3700448/1‑140 (MQ=255)
                                                                                                                      gTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGCAATTTTTCTGCCAAGAGACATTATCAAGTGAGGAACCCAGCACTCCCTTGCTGGGAGAGGCATCGATAAATTGGTTGTTGGTATCGTAAATACCTagctg               >  2:5047034/1‑136 (MQ=255)
                                                                                                                      gTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATg               >  2:5047109/1‑140 (MQ=255)
                                                                                                                       tCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCATTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGc              >  2:714744/1‑140 (MQ=255)
                                                                                                                       tCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGc              >  2:3399348/1‑140 (MQ=255)
                                                                                                                       tCCATTTTTCGACATACTAGATCCGTCTCGCCTGCCAGAACTTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGATGCGTGGATAAATTGGTTGTTGGTATCGTAAATTCCTACATGc              >  2:3752632/1‑140 (MQ=255)
                                                                                                                         cATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCaa            <  1:4973415/140‑1 (MQ=255)
                                                                                                                         cATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCaa            <  1:2631206/140‑1 (MQ=255)
                                                                                                                         cATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCaa            <  1:784185/140‑1 (MQ=255)
                                                                                                                                cGACATACTATATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCAttt     <  2:3047854/140‑1 (MQ=255)
                                                                                                                                 gACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCAtttt    >  2:3712547/1‑140 (MQ=255)
                                                                                                                                 gACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCAtttt    >  2:3712609/1‑140 (MQ=255)
                                                                                                                                   cATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAGTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGt  >  2:4561827/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGT  >  NZ_CP025268/1796949‑1797219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑