BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,808,384	A→G	pseudogene (474/1900 nt)	espL1 →	type III secretion system effector EspL1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,808,384	0	A	G	100.0%	858.3 / NA	255	pseudogene (474/1900 nt)	espL1	type III secretion system effector EspL1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (191/64); total (191/64)

Only 100 of 255 total aligned reads displayed.

TAACCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAATGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATAC  >  NZ_CP025268/1808249‑1808518

  aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                  >  2:621376/1‑140 (MQ=255)

  aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                  >  1:470670/1‑140 (MQ=255)

  aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                  >  2:5103836/1‑140 (MQ=255)

  aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                  >  1:5020452/1‑140 (MQ=255)

  aCCAGTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGGAGATCGTAACCATTGTATGACACTGTCTACACAAATCCATAGTGAACCAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                  >  2:4625839/1‑140 (MQ=255)

   ccATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGt                                                                                                                                 >  1:3828924/1‑140 (MQ=255)

   ccATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGt                                                                                                                                 >  2:4809066/1‑140 (MQ=255)

         gCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTg                                                                                                                           >  1:3712748/1‑140 (MQ=255)

             aGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAAt                                                                                                                       <  1:1936335/140‑1 (MQ=255)

              gCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAAtc                                                                                                                      >  1:213352/1‑140 (MQ=255)

                     ggATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAGAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCt                                                                                                               >  1:2935376/1‑140 (MQ=255)

                     ggATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCt                                                                                                               >  1:3121455/1‑140 (MQ=255)

                         tataTTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACg                                                                                                           >  1:165483/1‑140 (MQ=255)

                         tataTTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGCATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACg                                                                                                           >  1:78010/1‑140 (MQ=255)

                          atatTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGc                                                                                                          >  1:3553525/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTACAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                    >  2:4394782/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAACATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                    >  2:3632041/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                    >  1:4785194/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                    >  2:1527861/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                    >  2:34092/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTAGACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                    >  1:1969787/1‑140 (MQ=255)

                                     gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                               >  2:1637471/1‑140 (MQ=255)

                                     gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                               >  1:2881169/1‑140 (MQ=255)

                                     gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                               >  1:2491014/1‑140 (MQ=255)

                                     gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                               >  2:135781/1‑140 (MQ=255)

                                     gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                               >  1:3314945/1‑140 (MQ=255)

                                     gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                               >  2:3310837/1‑140 (MQ=255)

                                     gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                               >  1:1505594/1‑140 (MQ=255)

                                          gTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAAGTATACACGCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTTACGCTGTTTTTTGTCGCCAt                                                                                          >  2:4086697/1‑140 (MQ=255)

                                          gTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCAt                                                                                          >  2:827076/1‑140 (MQ=255)

                                          gTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCAt                                                                                          >  1:1582732/1‑140 (MQ=255)

                                          gTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCAt                                                                                          >  1:4064760/1‑140 (MQ=255)

                                          gTTAACTGCTGAAGATCGTAACCATTGTAGGAAACTGTCTAAAAAAATCCATAAAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGGCGCTATTTTTTGTCGCCAt                                                                                          >  1:4146322/1‑140 (MQ=255)

                                           ttAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATc                                                                                         >  1:3327936/1‑140 (MQ=255)

                                              caggctgAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTGTGTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGt                                                                                      <  1:3665377/137‑1 (MQ=255)

                                                tgctgAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCg                                                                                              >  1:2813046/1‑130 (MQ=255)

                                                tgctgAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCa                                                                                    >  1:4484103/1‑140 (MQ=255)

                                                  ctgAAGATCGTAACCATTGTGTGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCAtt                                                                                  >  2:5286308/1‑140 (MQ=255)

                                                     aaGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCa                                                                               >  1:2739067/1‑140 (MQ=255)

                                                     aaGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAG‑AAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCaa                                                                              >  1:2425267/1‑140 (MQ=255)

                                                        aTCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATa                                                                            <  2:2325653/140‑1 (MQ=255)

                                                        aTCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATa                                                                            <  1:3257572/140‑1 (MQ=255)

                                                         tagTAACCATTTTATGAATCTCTCTAAAAAAATCCATAGCGAGAAAAACTATACACCCAACAGTAAATCTCGATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCGATTGTTTGTCGCCATCTGTCATTGCAAtgc                                                                           <  2:2875171/138‑3 (MQ=255)

                                                          cGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACg                                                                          >  1:4930713/1‑140 (MQ=255)

                                                            tAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGcc                                                                        >  2:3206726/1‑140 (MQ=255)

                                                            tAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGcc                                                                        >  2:4106256/1‑140 (MQ=255)

                                                            tAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTGTTGTCGCCATCTGTCATTGCAATAcctg                                                                        >  2:3665377/1‑137 (MQ=255)

                                                               ccATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCtt                                                                     >  2:2185846/1‑140 (MQ=255)

                                                                  ttGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGAt                                                                  >  2:2521359/1‑140 (MQ=255)

                                                                  ttGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGAt                                                                  <  1:666967/140‑1 (MQ=255)

                                                                  ttGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGAt                                                                  >  1:969794/1‑140 (MQ=255)

                                                                   tGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCAtt                                                                                  <  2:5056214/123‑1 (MQ=255)

                                                                    gTATGAAAGGGTCTAAGAAAATCAATAGAGAAAAAAACTATACACCCTACAGTAAATCTCAATGGTGGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTTCAATACGCCCTGGAtgt                                                                >  2:4692957/1‑138 (MQ=255)

                                                                    gTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTc                                                                >  1:1411190/1‑140 (MQ=255)

                                                                       tGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTCACTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTCTTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGCTTCAtt                                                             >  1:5076361/1‑140 (MQ=255)

                                                                       tGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCAtt                                                             >  2:1163066/1‑140 (MQ=255)

                                                                          aaCTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTgag                                                          >  1:4293224/1‑140 (MQ=255)

                                                                             tGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCGGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGaaa                                                       >  1:3799733/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTTCAATCTCCTTCTGACGCTATTTTTTGTCTCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  2:4972672/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  1:85969/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  1:3525148/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  2:2834269/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  1:4819808/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  2:2013752/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  1:4937705/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  1:5199229/1‑140 (MQ=255)

                                                                                cTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATgg                                                    <  2:1351018/140‑1 (MQ=255)

                                                                                 tAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGa                                                   <  1:3458002/140‑1 (MQ=255)

                                                                                        aTCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCa                                            <  2:4484103/140‑1 (MQ=255)

                                                                                         tCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCaa                                           >  2:2778223/1‑140 (MQ=255)

                                                                                           cATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAg                                         >  1:3022325/1‑140 (MQ=255)

                                                                                                      aaaCTATACTCCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGAtt                              <  2:37795/140‑1 (MQ=255)

                                                                                                       acacataCACCAAACAATAAACATCAATGGTAGTGCATGTTTTTTGGAATCTCCTTCTGACGTTATTTTTTTTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAAGCGGATTa                             <  2:4723742/136‑1 (MQ=255)

                                                                                                          tagacaaCCAACAGTCAATCTCAAAGGTAGTCCATGCTTTTTGCAGTCTCCCTCTGACGCTATTTTTTGTCGGCGTCTGTCACTGCAACCCGCTCTTTCCTCATTGCGAAATGGAAAGGGCAAAGGCTACCTGATTAAAc                          >  2:4958340/4‑140 (MQ=255)

                                                                                                            taCACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACAt                        <  1:4683140/140‑1 (MQ=255)

                                                                                                                 ccAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActc                   >  1:1154963/1‑140 (MQ=255)

                                                                                                                   aaCAGTAAATCTCAATGGTAGTGGATATTTTTTGCAATCTCCTTCTGACGCAATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCGTTGTGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActcct                 >  1:3204403/1‑140 (MQ=255)

                                                                                                                   aaCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActcct                 >  1:3979107/1‑140 (MQ=255)

                                                                                                                   aaCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActcct                 >  1:3434391/1‑140 (MQ=255)

                                                                                                                    aCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTa                             >  2:4026434/1‑127 (MQ=255)

                                                                                                                    aCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTa                             <  1:4026434/127‑1 (MQ=255)

                                                                                                                    aCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActcctc                >  2:3358503/1‑140 (MQ=255)

                                                                                                                       gTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGt             <  2:454316/140‑1 (MQ=255)

                                                                                                                        tAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGtt            >  2:3959460/1‑140 (MQ=255)

                                                                                                                        tAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATATTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGtt            >  1:5162750/1‑140 (MQ=255)

                                                                                                                         aaaTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTg           >  1:2001239/1‑140 (MQ=255)

                                                                                                                           aTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGaa         >  1:3902080/1‑140 (MQ=255)

                                                                                                                           aTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGaa         >  2:219785/1‑140 (MQ=255)

                                                                                                                           aTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGaa         >  2:29475/1‑140 (MQ=255)

                                                                                                                            tctcAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGCCGCGATGTTGTGTCGCCATCGGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAAt        <  1:2185977/140‑1 (MQ=255)

                                                                                                                            tctcAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAAt        <  1:4404605/140‑1 (MQ=255)

                                                                                                                            tctcAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAAt        <  2:1969787/140‑1 (MQ=255)

                                                                                                                            tctcAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAAt        <  2:1582798/140‑1 (MQ=255)

                                                                                                                            tctcAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAAt        <  1:1012681/140‑1 (MQ=255)

                                                                                                                               cAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAACAGGCAAAGTCAACCTGATTACACATTACTCCTCCGTTGAATCCa     <  2:3857036/140‑1 (MQ=255)

                                                                                                                                aaTGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCAt    >  2:2701139/1‑140 (MQ=255)

                                                                                                                                aaTGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCAt    >  1:2630547/1‑140 (MQ=255)

                                                                                                                                aaTGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCAt    >  2:4345473/1‑140 (MQ=255)

                                                                                                                                aaTGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTGTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTTAACATTAGTCCTCCGTTGAATCCAt    >  2:4845148/1‑140 (MQ=255)

                                                                                                                                 aTGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATa   <  2:3553525/140‑1 (MQ=255)

TAACCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAATGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATAC  >  NZ_CP025268/1808249‑1808518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`