BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,957,477	A→G	132 (TAA→TAG)	yecN →	MAPEG family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,957,477	0	A	G	99.2%	798.0 / NA	241	132 (TAA→TAG)	yecN	MAPEG family protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (127/110); total (129/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

ATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATC  >  NZ_CP025268/1957340‑1957613

  gCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGca                                                                                                                                      <  1:306249/140‑1 (MQ=255)

     ttattaCGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACTTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTACCTGCGTTAGCGCACaa                                                                                                                                   >  2:1858061/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGCCGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActtt                                                                                                                        >  1:2177760/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActtt                                                                                                                        >  1:2654442/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActtt                                                                                                                        >  1:4293891/1‑140 (MQ=255)

                  catcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActttct                                                                                                                      >  1:3317843/1‑140 (MQ=255)

                  catcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActttct                                                                                                                      >  2:3844180/1‑140 (MQ=255)

                   atcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActttctt                                                                                                                     >  2:3408516/1‑140 (MQ=255)

                        cGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTcc                                                                                                                >  1:3234901/1‑140 (MQ=255)

                         gTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGTGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTccc                                                                                                               <  2:3175290/140‑1 (MQ=255)

                          tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCg                                                                                                              >  1:3565601/1‑140 (MQ=255)

                          tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCg                                                                                                              >  1:5256985/1‑140 (MQ=255)

                          tCTGTTCCGCTGGCGACGATTCGGCATGAGCGCCCCCTGGTGTGCGCGGTTGCTGATGATGCTGGCGAAACTTTGGTATATGCCCTGGGGGTTGGTTTTCTCCCTGCGTAAGCGCACAATACGCGACTTTCTTTTTccag                                                                                                              >  2:4052808/1‑138 (MQ=255)

                                  gCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTa                                                                                                      >  2:4341690/1‑140 (MQ=255)

                                    tGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACg                                                                                                    >  1:2271135/1‑140 (MQ=255)

                                      gCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGtt                                                                                                  >  1:4209531/1‑140 (MQ=255)

                                      gCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGtt                                                                                                  >  1:1918334/1‑140 (MQ=255)

                                      gCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGtt                                                                                                  <  1:5084483/140‑1 (MQ=255)

                                      gCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGtt                                                                                                  <  1:5327735/140‑1 (MQ=255)

                                            ttCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGtct                                                                                            <  1:1075783/140‑1 (MQ=255)

                                                     gcgcgcCACCCGGTGTGCGCTGCTGCTTATTGTGCTGGCGAATCTTTGGTATATGCCCCGGGAGTTGGTTTTCCCCCTGCGTTCGCCCACAATACCCCACTTTCTTTTTCCCGGATTTTTCCGTTATGTCTCCCCGCGac                                                                                   <  2:2465528/138‑1 (MQ=255)

                                                     gAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGCGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGac                                                                                   <  1:4433500/140‑1 (MQ=255)

                                                      aGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGaca                                                                                  >  2:466696/1‑140 (MQ=255)

                                                          ccACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                              <  1:1775746/140‑1 (MQ=255)

                                                          ccACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                              <  1:3925722/140‑1 (MQ=255)

                                                          ccACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                              <  1:4146698/140‑1 (MQ=255)

                                                                  tgtgCGCTGTTGCTGATGGTGCTGGCTAATCTTTGGTATATGCGCTGGGAGTTGGTTTTCTGCCTGCGTTAGCGGACAATACGCTACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCATCGCGACACGCTATTTTCtt                                                                      >  2:4229954/1‑139 (MQ=255)

                                                                  tgtgCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTg                                                                      <  2:589222/140‑1 (MQ=255)

                                                                  tgtgCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTg                                                                      >  1:5013643/1‑140 (MQ=255)

                                                                     gcgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGccc                                                                   >  2:4834172/1‑140 (MQ=255)

                                                                     gcgcTGTTGCTGAAGGTGCTGGCGAATCTTTGGGATATGCCCTGGGCGTTGGTTTTCACCCTGCGTTAGCGCACAATACGCAACTTTCTTTTTCCCGGGTTTTTACGTTATCTCTCATCGCGCCACGCTATTTTCTGccc                                                                   >  2:3450945/1‑140 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATCCGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  2:3690141/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  1:3901189/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  >  1:4364779/1‑140 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  1:2539720/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  2:1283135/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  >  2:2280086/1‑140 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  2:3285363/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTCCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  1:4780665/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTAGGTCTATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  2:3743805/140‑1 (MQ=255)

                                                                       gcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCt                                                                 >  2:3500631/1‑140 (MQ=255)

                                                                        cTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTa                                                                >  1:1764534/1‑140 (MQ=255)

                                                                        cTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTa                                                                >  1:2401852/1‑140 (MQ=255)

                                                                        cTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGAGTTTTACGTTATGTCTCACCGCGACAAGCTATTTTCTGCCCCTa                                                                >  2:1179489/1‑140 (MQ=255)

                                                                           ttGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCg                                                             >  2:2537604/1‑140 (MQ=255)

                                                                           ttGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCg                                                             >  1:659482/1‑140 (MQ=255)

                                                                           ttGCTGATGGTGCTGGCGAATCATTGGTATATGCCCTGGGAGCTGGGTTTCTCCCTGCGTCAGCGCACAGCACGCCTCTTCCTCGCTCCCGGACTCTTACGTCATGTCTCACTGCGACCCGCCGTTGTCTGCCACTGTCg                                                             >  2:5355182/1‑140 (MQ=255)

                                                                                gATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGa                                                        <  1:5155513/140‑1 (MQ=255)

                                                                                 aTGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGAc                                                       <  2:2794733/140‑1 (MQ=255)

                                                                                   ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTg                                                     >  2:746368/1‑140 (MQ=255)

                                                                                   ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTg                                                     <  2:2899859/140‑1 (MQ=255)

                                                                                   ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTg                                                     >  1:4647253/1‑140 (MQ=255)

                                                                                   ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTg                                                     >  2:3735514/1‑140 (MQ=255)

                                                                                     tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                   >  1:2588484/1‑140 (MQ=255)

                                                                                     tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                   >  2:1431140/1‑140 (MQ=255)

                                                                                     tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                   >  1:2144264/1‑140 (MQ=255)

                                                                                       cTGGCGAATCTTTGGTATATGCCTTGGGAGTGGGAGTTCCTCCTGCGATAGCGCACAATCCGTCACTTTCTTTTTCCAGGAGGTTTACGTTATGTCTCACCGTGACACGCTAATTTCTGCCCCTATCGCCAGaatagccc                                                 >  2:2023186/1‑133 (MQ=255)

                                                                                        tGGCGAATCTTTGGTATATGCCCTGGGAGTTGGCTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGa                                                >  2:2684797/1‑140 (MQ=255)

                                                                                           cGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTg                                             <  2:1175694/140‑1 (MQ=255)

                                                                                           cGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTg                                             <  2:5256985/140‑1 (MQ=255)

                                                                                               tCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGAcc                                         >  1:899221/1‑140 (MQ=255)

                                                                                                    ggTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTtga                                    <  2:1242954/140‑1 (MQ=255)

                                                                                                     ctataTGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTtgat                                   <  1:1942067/139‑1 (MQ=255)

                                                                                                        tatGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGaa                                <  1:665179/140‑1 (MQ=255)

                                                                                                         atGCCCTGGGAGTTGGTTTTCACTCTGCGTTAGCGCACAATACGCCACTTTGTTCTTCCCGGATTTTTACGTTATGTGTCACCCCCACACGCTATTTTCTGCCCCTGTCGCCAGACTCGGGGACTGGGTCTTTGATGAAc                               >  2:5191500/1‑140 (MQ=255)

                                                                                                          tGCCCTGTGAGTGGGTTGTGTCCCTGCGTTAGCGCCCGCTACGCCGCTTTCTGTTTCCCGGATTTTTAGGTTACGTCGCACCGCGACCCGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACg                              <  2:3335421/140‑1 (MQ=255)

                                                                                                          tGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGCTGAACg                              >  2:1534511/1‑140 (MQ=255)

                                                                                                          tGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACg                              <  1:185087/140‑1 (MQ=255)

                                                                                                             ccTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGt                           >  1:786248/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACCATACGCCACTTTCTTTTTCCGGGCTTTTTTCGTTATGTCTCACCCCGGCACGCGATTTTCTGCCCCTATCGCCTGACTGGGCGACCGGCCCTTTGATGGTCGGTTAgg                        >  2:1605499/1‑139 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                        >  1:3430012/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                        >  1:4225669/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                        >  1:4629505/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                        >  1:578099/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                        >  1:974718/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                        >  2:1632697/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                        >  2:1743552/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCCTTGATGAACGGGTAGc                        >  1:2967986/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGCTAGCGCACAATACGTCACTTTATTTTTCCGGGATGTTTACGGTATGTCTCACGGCGACACGCTATTTTCTGCCCCTATCGCCTGACTGGGTGACTGGACCTTTGATGAACGGGTAGc                        >  2:4656654/1‑140 (MQ=255)

                                                                                                                gggAGTTGAGTTTCTCCCCGCGTTAGCGCACAATCCGGCACTTGCTTTGTCGCTGGTTTAGACGTGATGACTCGCCGCGACACCCTATTTTCTGCCATTATCGCCAGACTGGGCGACAGGACCTTTGATGAActcgtggc                        >  2:884576/1‑133 (MQ=255)

                                                                                                                  gAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTg                      <  2:1850440/140‑1 (MQ=255)

                                                                                                                     ttGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAg                   >  2:1221875/1‑140 (MQ=255)

                                                                                                                      tGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt                  <  1:1481149/140‑1 (MQ=255)

                                                                                                                      tGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt                  <  1:283014/140‑1 (MQ=255)

                                                                                                                      tGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt                  >  2:4420499/1‑140 (MQ=255)

                                                                                                                      tGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt                  <  2:5058428/140‑1 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc                 >  2:213029/1‑140 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc                 >  2:4173712/1‑140 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc                 >  1:2497729/1‑140 (MQ=255)

                                                                                                                          tttCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCTGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTc              <  1:3844180/140‑1 (MQ=255)

                                                                                                                          tttCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTc              <  1:3470849/140‑1 (MQ=255)

                                                                                                                          tttCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTc              <  1:4031028/140‑1 (MQ=255)

                                                                                                                           ttCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTcc             <  1:2189047/140‑1 (MQ=255)

                                                                                                                             ctcCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCg           >  1:1332361/1‑140 (MQ=255)

                                                                                                                             ctcCCTGCGTTACCGCACAATACGCCACTTTCTTTTTCCCGGCTTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACAGGTAGCTGAAGTCTTCCCg           >  2:2873646/1‑140 (MQ=255)

                                                                                                                               cccTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGa         >  1:1124316/1‑140 (MQ=255)

                                                                                                                                 cTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGata       <  1:4860949/140‑1 (MQ=255)

                                                                                                                                 cTGCGTTAGCGCACAATACGCCACTTTCTTGTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTCTTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGata       <  1:1017020/140‑1 (MQ=255)

                                                                                                                                     gTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGAt   <  2:5320117/140‑1 (MQ=255)

                                                                                                                                      ttAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATc  <  2:3084712/140‑1 (MQ=255)

ATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATC  >  NZ_CP025268/1957340‑1957613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`