BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,966,179	C→A	L434L (CTC→CTA)	argS →	arginine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,966,179	0	C	A	92.5%	1784.2 / 65.9	511	L434L (CTC→CTA)	argS	arginine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (12/26); new base A (154/317); total (167/344)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 512 total aligned reads displayed.

TGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAG  >  NZ_CP025268/1966040‑1966314

    aCTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCa                                                                                                                                     >  2:3519559/1‑140 (MQ=255)

     cTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCaa                                                                                                                                    >  1:3335112/1‑140 (MQ=255)

     cTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGATTGGTATTGGTGCGGTGAAATATGCGGAGCTATCCaa                                                                                                                                    >  2:1831998/1‑140 (MQ=255)

      tGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATGGGTGCGGTGAAATATGCGGATCTATCCaaa                                                                                                                                   <  1:2447870/140‑1 (MQ=255)

        gCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGCACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTCACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTCTCCaaaaa                                                                                                                                 >  2:4298654/1‑140 (MQ=255)

        gCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAATTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCaaaaa                                                                                                                                 >  2:5040753/1‑140 (MQ=255)

         ccGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAAc                                                                                                                                <  1:1791428/140‑1 (MQ=255)

          cGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAAcc                                                                                                                               <  2:2018616/140‑1 (MQ=255)

          cGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAAcc                                                                                                                               <  2:2808021/140‑1 (MQ=255)

             tCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGca                                                                                                                            <  1:2091040/140‑1 (MQ=255)

               tgctgGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTCTCCAAAAACCGcacc                                                                                                                          <  1:2803905/140‑1 (MQ=255)

               tgctgGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGcacc                                                                                                                          <  2:1705534/140‑1 (MQ=255)

                gctgGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGcacca                                                                                                                         <  1:5190661/140‑1 (MQ=255)

                gctgGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGcacca                                                                                                                         <  2:2868742/140‑1 (MQ=255)

                      tGAAGCCCTGGTACTTGCCCGCAGTCTGGGCGCAGAATAGAACCCTGATATGCCAGCCGCAGAGCTGGAAAAACTGGCTAACGCGGTTTGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACt                                                                                                                   <  2:3108299/140‑1 (MQ=255)

                      tGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACt                                                                                                                   <  1:3881774/140‑1 (MQ=255)

                      tGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACt                                                                                                                   <  2:1983232/140‑1 (MQ=255)

                      tGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACt                                                                                                                   <  1:4528619/140‑1 (MQ=255)

                      cgAAGCCCTGGAACGTGCCCGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATACGCGGATCTATCCAAAAACCGCACCACGGACt                                                                                                                   <  2:1083464/139‑1 (MQ=255)

                                 aaCGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGAc                                                                                                        <  1:522182/140‑1 (MQ=255)

                                 aaCGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGAc                                                                                                        <  1:2687411/140‑1 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  2:4457914/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  1:78323/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  2:1547134/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  1:1026054/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  2:189474/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  1:4834964/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  2:2081564/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  2:2332203/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  2:3996802/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  1:3719655/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  1:1673896/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  1:2396569/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  1:3650552/1‑140 (MQ=255)

                                     tGCACGCCGTCTGGTGGCAGAAAAGAACCCGGACATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTggg                                                                                                    >  1:2744484/1‑140 (MQ=255)

                                        aCGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGaca                                                                                                 <  1:4112986/140‑1 (MQ=255)

                                              tCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAAGTGGCTAACGCGGTTGGTATGAGTGCGGTGAAGTATGTGGATCTATCCAAAAACCGCAGCACGTACGACATGTTCAACTGGGACAACAttg                                                                                           >  2:19465/1‑138 (MQ=255)

                                              tCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGCATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGc                                                                                           >  1:1841359/1‑140 (MQ=255)

                                               cTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCt                                                                                          >  1:2210561/1‑140 (MQ=255)

                                                    ggCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGt                                                                                     <  2:1629548/140‑1 (MQ=255)

                                                      cAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGttt                                                                                   <  2:3332188/140‑1 (MQ=255)

                                                      cAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGttt                                                                                   <  1:130692/140‑1 (MQ=255)

                                                      cAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGttt                                                                                   <  1:5080442/140‑1 (MQ=255)

                                                      cAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGttt                                                                                   <  1:1366452/140‑1 (MQ=255)

                                                      cAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGttt                                                                                   <  1:2195110/140‑1 (MQ=255)

                                                        gAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGa                                                                                 >  1:5010027/1‑140 (MQ=255)

                                                        gAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGa                                                                                 >  2:796829/1‑140 (MQ=255)

                                                           aaGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAggg                                                                              <  2:4993234/140‑1 (MQ=255)

                                                                    gATACGCCAGCCGACGAGCTGGAAAAACTAGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACcgc                                                                     <  1:3529035/140‑1 (MQ=255)

                                                                      tatGCCAGCCGACGAGCTGGAAAACCTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCgcgc                                                                   <  1:4062171/140‑1 (MQ=255)

                                                                      tatGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCgcgc                                                                   <  2:2562925/140‑1 (MQ=255)

                                                                      tatGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCCATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCgcgc                                                                   <  2:4933594/140‑1 (MQ=255)

                                                                         gCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGccct                                                                >  2:4611007/1‑138 (MQ=255)

                                                                           cAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATAc                                                              <  1:3078486/140‑1 (MQ=255)

                                                                            aGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACa                                                             <  1:979098/140‑1 (MQ=255)

                                                                              ccGACGAGCTGGAAAATCTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGAACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATg                                                           <  1:2953861/140‑1 (MQ=255)

                                                                              ccGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATg                                                           <  1:1514432/140‑1 (MQ=255)

                                                                              ccGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATg                                                           >  2:5299236/1‑140 (MQ=255)

                                                                               cgacgaGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGc                                                          <  1:4232886/140‑1 (MQ=255)

                                                                               cgacgaGCTAGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGc                                                          <  1:3776779/140‑1 (MQ=255)

                                                                                gacgaGCTGGAAAACCTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATCCATGCa                                                         <  1:19156/140‑1 (MQ=255)

                                                                                gacgaGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCa                                                         <  1:2483986/140‑1 (MQ=255)

                                                                                  cgaGCTGGAAAAACTGGCTGACGCGGTTGGTATTGGGGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTCGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGt                                                       <  2:248657/140‑1 (MQ=255)

                                                                                  cgaGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGt                                                       <  1:3146844/140‑1 (MQ=255)

                                                                                  cgaGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGt                                                       <  1:922126/140‑1 (MQ=255)

                                                                                  cgaGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGt                                                       <  1:2130946/140‑1 (MQ=255)

                                                                                    ggCTGGAAGAGCTGGCTAACGGGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTAt                                                     <  2:3719386/139‑1 (MQ=255)

                                                                                        ggAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTGTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGGTTGAGGGTAATACCGCGCCATACATGCAGTATGCAt                                                 >  1:4842700/1‑140 (MQ=255)

                                                                                         gAAAAACTGGCTAACGTGGTTGGTATTGGTGCGGTGGAATATGCGGATCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCaca                                                <  1:4280907/140‑3 (MQ=255)

                                                                                         gAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATa                                                <  1:234676/140‑1 (MQ=255)

                                                                                         gAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATa                                                <  2:1271017/140‑1 (MQ=255)

                                                                                         gAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATa                                                <  1:734142/140‑1 (MQ=255)

                                                                                         gAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATa                                                <  2:2683376/140‑1 (MQ=255)

                                                                                         gAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATa                                                <  2:3054607/140‑1 (MQ=255)

                                                                                           aaaaCTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATaca                                              <  2:2178828/140‑1 (MQ=255)

                                                                                           aaaaCTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATaca                                              <  2:4658350/140‑1 (MQ=255)

                                                                                           aaaaCTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATaca                                              <  1:5135813/140‑1 (MQ=255)

                                                                                           aaaaCTGGCTAACGCGGTTGGTATTGGTGCGGTGAAAGATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATaca                                              <  1:3583518/140‑1 (MQ=255)

                                                                                           aaaaCTGGCTAACGCGGTTGGTATTAGTGCGGTGAAATAGGAGGAGATATCCAAGAACCGCACCACGGACTACATCGTCGCCAGGGACAACAGGCGGGCGTGTGACGGTAATACCGCGCCATACATGAAGTATGCATacc                                              <  2:3202194/140‑2 (MQ=255)

                                                                                              aCTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACAcgc                                           <  2:883632/140‑1 (MQ=255)

                                                                                               cTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACAcgcg                                          <  1:116068/140‑1 (MQ=255)

                                                                                                   cTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTa                                      >  1:3407306/1‑140 (MQ=255)

                                                                                                    tAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTAt                                     <  2:3624365/140‑1 (MQ=255)

                                                                                                    tAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTAt                                     <  1:5305935/140‑1 (MQ=255)

                                                                                                            ttGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGttt                                                                                   >  1:393509/1‑86 (MQ=255)

                                                                                                            ttGGTATTGGTGCGGTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCgtg                             >  2:2440132/1‑140 (MQ=255)

                                                                                                              ggTATTGGTGCGGTGAAATATGCGGTTCTATCCAAAAACCGCACCACGGACTACATCTCCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGtt                           <  2:4830556/140‑1 (MQ=255)

                                                                                                                      tGCGGTGCAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGGCTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAg                   <  2:3565181/140‑1 (MQ=255)

                                                                                                                          gTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGa               >  2:135332/1‑140 (MQ=255)

                                                                                                                          gTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGa               >  2:593659/1‑140 (MQ=255)

                                                                                                                          gTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGa               >  2:3789063/1‑140 (MQ=255)

                                                                                                                          gTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGa               >  1:3280326/1‑140 (MQ=255)

                                                                                                                          gTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGa               >  2:939513/1‑140 (MQ=255)

                                                                                                                          gTGAAATATGCGGATCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTAGGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGa               >  2:410560/1‑140 (MQ=255)

                                                                                                                                  tGCGGATCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATa                                                >  1:4622719/1‑99 (MQ=255)

                                                                                                                                  cgccgATCTATCCAAAAACCGCACCACGGACTACATCTTCGACCGGGCCAACATGCCGGCGTAAGAGGGTAATACCGCGCCATACACGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGAAATTGACg       <  2:4221771/136‑1 (MQ=255)

                                                                                                                                    cGGATCTATCCAAAAACCGCACCACGGCCTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGAAATTGACGaa     <  1:572817/140‑1 (MQ=255)

                                                                                                                                       aTCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAagag  >  2:1492749/1‑140 (MQ=255)

                                                                                                                                       aTCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAagag  <  1:4936733/140‑1 (MQ=255)

                                                                                                                                       aTCTATCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAagag  >  1:4378307/1‑140 (MQ=255)

TGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTGGCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAG  >  NZ_CP025268/1966040‑1966314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`