BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,038,575	A→G	pseudogene (486/1193 nt)	ompC →	porin OmpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,038,575	0	A	G	99.7%	1178.8 / NA	359	pseudogene (486/1193 nt)	ompC	porin OmpC
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (218/139); minor base T (1/0); total (220/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 359 total aligned reads displayed.

GGTGTCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAA  >  NZ_CP025268/2038438‑2038708

ggTGTCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGt                                                                                                                                     >  2:722132/1‑140 (MQ=255)

ggTGTCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGt                                                                                                                                     >  2:2740209/1‑140 (MQ=255)

  tgtCGTATCTGCCGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCg                                                                                                                                   <  2:4567709/140‑1 (MQ=255)

  tgtCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCg                                                                                                                                   <  1:3420430/140‑1 (MQ=255)

    tCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGTCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACTGTAACAATGGCTTCTTTGGTTAGGTCGAt                                                                                                                                 >  1:4260572/1‑140 (MQ=255)

    tCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGAt                                                                                                                                 >  2:291715/1‑140 (MQ=255)

    tCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGAt                                                                                                                                 >  2:2242946/1‑140 (MQ=255)

    tCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGAt                                                                                                                                 >  2:394328/1‑140 (MQ=255)

    tCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTAAGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGTTTCTTTGGTTAGGTCGAt                                                                                                                                 >  2:3806476/1‑140 (MQ=255)

       tataTGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGc                                                                                                                              >  2:3674548/1‑140 (MQ=255)

         tatGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCtt                                                                                                                            >  1:3803258/1‑140 (MQ=255)

          atGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTg                                                                                                                           >  1:3143349/1‑140 (MQ=255)

              cGTAGGAGCATGGACCGATATGCTGTCATCATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGCACTGTCGTTCTCATGGTGTGGCAACCTAACGTAACAACGGCTGCTTTGGTTAGGTGGATGGATTGAAct                                                                                                                       >  2:4365813/1‑140 (MQ=255)

              cGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATTTTGTCGCGGACAACTTCATGAACTGTGGTGCTAATGGTGTTGCAACCTACCGTAACAATGTCTTCTTTGTTTAGGTCGATGGTTGGaaat                                                                                                                       >  2:2344769/1‑138 (MQ=255)

              cGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAAct                                                                                                                       >  1:1950353/1‑140 (MQ=255)

              cGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAAct                                                                                                                       >  1:4077815/1‑140 (MQ=255)

              cGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAAct                                                                                                                       >  1:2442173/1‑140 (MQ=255)

              cGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAAct                                                                                                                       >  2:2036946/1‑140 (MQ=255)

              cGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAAct                                                                                                                       >  1:5149666/1‑140 (MQ=255)

                 aGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGTGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACATACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAActttg                                                                                                                    >  1:3186143/1‑140 (MQ=255)

                  gAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAActttgc                                                                                                                   <  2:401512/140‑1 (MQ=255)

                   aaGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAActttgct                                                                                                                  >  2:3709445/1‑140 (MQ=255)

                   aaGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAActttgct                                                                                                                  >  2:786915/1‑140 (MQ=255)

                   aaGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAActttgct                                                                                                                  >  1:5006283/1‑140 (MQ=255)

                        tGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCCACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCa                                                                                                             >  2:4149588/1‑140 (MQ=255)

                        tGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCa                                                                                                             >  1:4477050/1‑140 (MQ=255)

                        tGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCa                                                                                                             >  1:3465027/1‑140 (MQ=255)

                        tGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCa                                                                                                             >  1:2495159/1‑140 (MQ=255)

                        tGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCa                                                                                                             >  2:1971274/1‑140 (MQ=255)

                        tGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGGTGCAACCTACCGTAACAATGGCTTCCTTTGTTAGGTCGATGGCTTGAACTTTGCTTTGCa                                                                                                             >  2:2601140/1‑140 (MQ=255)

                        tGGACCGATATGCCGCAAGAATATGGTGGCGATACCTATGCTGGCGCTGACTAATTCATGAACGGTCGTGCTAATGGTGTTGCAACTTTACGTAACAGTGGCTTCTGTGGTTATATCGATGGCTTGAActttgctgggca                                                                                                             >  2:5333507/1‑135 (MQ=255)

                          gACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAat                                                                                                           >  1:1065349/1‑140 (MQ=255)

                           aCCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAata                                                                                                          >  1:3251576/1‑140 (MQ=255)

                            ccGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAatat                                                                                                         <  1:5320105/140‑1 (MQ=255)

                              gATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCa                                                                                                       <  1:2922725/140‑1 (MQ=255)

                               atatGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGc                                                                                                                              >  1:924421/1‑116 (MQ=255)

                               atatGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGc                                                                                                                              <  2:924421/116‑1 (MQ=255)

                               atatGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATAAACGGTAGGGCTATTGGTGTTGCAACCTACCGTAACAATGGGTTCTTTGGTTAGTTAGATGTCCAGAACTTTGCTTGGCAatacaag                                                                                                      >  2:3141502/1‑136 (MQ=255)

                                tatGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAgg                                                                                                     <  2:4283414/140‑1 (MQ=255)

                                 atGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAggg                                                                                                    >  1:1007686/1‑140 (MQ=255)

                                     tgcCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGtaat                                                                                                >  2:4748806/1‑140 (MQ=255)

                                     tgcCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGtaat                                                                                                >  2:3258105/1‑140 (MQ=255)

                                     tgcCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGtaaa                                                                                                >  1:4719552/1‑139 (MQ=255)

                                      gcCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGtaata                                                                                               <  2:5102022/140‑1 (MQ=255)

                                         aGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATg                                                                                            >  2:1985278/1‑140 (MQ=255)

                                          gAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACTGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGa                                                                                           >  1:4009750/1‑140 (MQ=255)

                                          gAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGa                                                                                           <  1:263397/140‑1 (MQ=255)

                                          gAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGa                                                                                           <  2:1085468/140‑1 (MQ=255)

                                                ggtggCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCGTTGCAATATCAGGGTAATAATGAAAgggg                                                                                     >  1:3927942/1‑140 (MQ=255)

                                                    gCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGa                                                                                 <  1:1463921/140‑1 (MQ=255)

                                                    gCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGa                                                                                 <  1:2544586/140‑1 (MQ=255)

                                                    gCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGa                                                                                 <  2:1509241/140‑1 (MQ=255)

                                                     cGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGAc                                                                                >  1:2591136/1‑140 (MQ=255)

                                                     cGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGGGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTAGAGGGCTTGAACTTTGCTTGGCAATATCAGGGTCATAATGAAAGTGGTGGAc                                                                                >  2:3343056/1‑140 (MQ=255)

                                                       aTACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCa                                                                              >  1:571682/1‑140 (MQ=255)

                                                         aCCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCAtt                                                                            <  2:3047610/140‑1 (MQ=255)

                                                              tGCTGGCGCTGCCAACTTCAGGAACGGTCGTGCTAACGGTGTCGCCACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTCGAACTTTGCGTCGCAATATCAGAGTAATAATGAAAGTGCTGGCCCATTctgcc                                                                       >  2:1159166/1‑136 (MQ=255)

                                                              tGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGc                                                                       >  1:685882/1‑140 (MQ=255)

                                                                 tGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAgg                                                                    <  2:354769/140‑1 (MQ=255)

                                                                   gcgcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGaa                                                                  >  1:5238780/1‑140 (MQ=255)

                                                                   gcgcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGaa                                                                  >  2:2051880/1‑140 (MQ=255)

                                                                   gcgcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGaa                                                                  >  1:82165/1‑140 (MQ=255)

                                                                   gcgcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGaa                                                                  <  2:2816443/140‑1 (MQ=255)

                                                                   gcgcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATCCGGGCAGGaa                                                                  >  1:3301427/1‑140 (MQ=255)

                                                                   gcgcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTAGAACTTTGCTTTGCAATATCAGGGTAATAATGGAAGTGGTGGACCATTCGGGCAGGaa                                                                  >  2:3528003/1‑140 (MQ=255)

                                                                    cgcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAgg                                                                    >  2:67243/1‑137 (MQ=255)

                                                                     gcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAgg                                                                >  1:3679562/1‑140 (MQ=255)

                                                                     gcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAgg                                                                >  1:1432312/1‑140 (MQ=255)

                                                                      cTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCCGGAAGGt                                                               <  2:346154/140‑1 (MQ=255)

                                                                        gACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTc                                                             <  2:4478080/140‑1 (MQ=255)

                                                                        gACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTc                                                             >  2:1308203/1‑140 (MQ=255)

                                                                                cATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAgtg                                                     >  1:785693/1‑140 (MQ=255)

                                                                                     aCGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGAt                                                >  2:4865083/1‑140 (MQ=255)

                                                                                        gTCGTGCTAATGGTGTTGCAACCTACCGTAACCATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGc                                             >  2:600678/1‑140 (MQ=255)

                                                                                        gTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGc                                             >  1:268176/1‑140 (MQ=255)

                                                                                        gTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGc                                             >  1:2049070/1‑140 (MQ=255)

                                                                                        gTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGGCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGc                                             >  1:504318/1‑140 (MQ=255)

                                                                                            tGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGCTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTa                                         >  2:4642019/1‑140 (MQ=255)

                                                                                              cTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGc                                       >  1:971133/1‑140 (MQ=255)

                                                                                                  tGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCttt                                   <  2:181417/140‑1 (MQ=255)

                                                                                                   ggTGTTGCAAGCTACCGTAACAATGGCTTCGTTGGTTAGGTGGATGGCTTGAACTTTGCTGGGCAATATCAGGGTAATAATGAAAGGGGTGGACCATTCGGGCAGGAAGGTTCCGGTGGTGGTGATGGCCGTAGCCtttg                                  >  2:143800/1‑139 (MQ=255)

                                                                                                   ggTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTc                                  >  1:4635712/1‑140 (MQ=255)

                                                                                                   ggTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTc                                  >  1:1109795/1‑140 (MQ=255)

                                                                                                   ggTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTc                                  >  1:2176497/1‑140 (MQ=255)

                                                                                                   ggTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTc                                  >  2:2455801/1‑140 (MQ=255)

                                                                                                   ggTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTc                                  >  2:1326229/1‑140 (MQ=255)

                                                                                                      gtTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCaa                               >  1:1240391/1‑140 (MQ=255)

                                                                                                       ttGCAACCTACCGTAACAATGGCTTCTGTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCaaa                              >  2:3937028/1‑140 (MQ=255)

                                                                                                               tACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATgg                      >  1:4602389/1‑140 (MQ=255)

                                                                                                                       cAATGGCTTCCTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGtt              <  2:700715/140‑1 (MQ=255)

                                                                                                                         aTGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGACGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTc            <  1:1738220/140‑1 (MQ=255)

                                                                                                                         aTGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTc            >  1:1603630/1‑140 (MQ=255)

                                                                                                                         aTGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTc            <  1:2740209/140‑1 (MQ=255)

                                                                                                                         aTGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTc            <  1:4326785/140‑1 (MQ=255)

                                                                                                                           ggCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCgg          <  2:3186143/140‑1 (MQ=255)

                                                                                                                            gCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGt         <  1:5110527/140‑1 (MQ=255)

                                                                                                                             cttcttTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTa        <  1:4134680/140‑1 (MQ=255)

                                                                                                                             cttcttTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTa        <  2:167165/140‑1 (MQ=255)

                                                                                                                                   tGGTTAGGTCGCTGCCTTGAACTTTGATTTGCAATCTCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCaa  <  2:4087674/140‑1 (MQ=255)

                                                                                                                                   tGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCaa  <  1:4865083/140‑1 (MQ=255)

GGTGTCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAA  >  NZ_CP025268/2038438‑2038708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`