BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,122,203	A→G	C81R (TGT→CGT)	wcaK ←	colanic acid biosynthesis pyruvyl transferase WcaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,122,203	0	A	G	100.0%	960.3 / NA	280	C81R (TGT→CGT)	wcaK	colanic acid biosynthesis pyruvyl transferase WcaK
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (121/159); total (121/159)

Only 100 of 284 total aligned reads displayed.

CCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATCACATCCACT  >  NZ_CP025268/2122067‑2122339

               aTGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCCGGTAGCGGCGACGGAGGACTTTTTTAAcgcg                                                                                                                        <  2:4073127/140‑1 (MQ=255)

                tGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACgcgc                                                                                                                       >  2:4353205/1‑140 (MQ=255)

                    gtcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGGCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCTACGGAGGACTTTTTTATCGCGCCCGa                                                                                                                   >  1:4222810/1‑140 (MQ=255)

                     tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCTTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTGTTTTAACGCGCCCGAc                                                                                                                  >  1:2969671/1‑140 (MQ=255)

                     tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGAc                                                                                                                  >  2:384374/1‑140 (MQ=255)

                     tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGAc                                                                                                                  >  2:2245008/1‑140 (MQ=255)

                     tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGAc                                                                                                                  >  1:849773/1‑140 (MQ=255)

                     tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGAc                                                                                                                  >  1:2440067/1‑140 (MQ=255)

                     tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTCATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGAc                                                                                                                  >  2:5137599/1‑140 (MQ=255)

                              gACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAcgcccgc                                                                                                         >  1:3311349/1‑140 (MQ=255)

                               aCAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAcgcccgcc                                                                                                        >  2:939098/1‑140 (MQ=255)

                                cagccgGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                       <  1:3833071/140‑1 (MQ=255)

                                cagcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                       <  2:2279564/140‑1 (MQ=255)

                                cagcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                       <  2:1692706/140‑1 (MQ=255)

                                cagcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                       <  1:2514752/140‑1 (MQ=255)

                                  gcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                                     <  1:1289753/140‑1 (MQ=255)

                                  gcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                                     <  1:3600757/140‑1 (MQ=255)

                                  gcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                                     <  2:1297641/140‑1 (MQ=255)

                                  gcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                                     <  2:1333629/140‑1 (MQ=255)

                                  gcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                                     <  1:3914608/140‑1 (MQ=255)

                                  gcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                                     <  2:2716408/140‑1 (MQ=255)

                                   cagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCgcgc                                                                                                    >  1:113978/1‑140 (MQ=255)

                                     ggCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtg                                                                                                  >  1:4567654/1‑140 (MQ=255)

                                         cACGAAGTCGGTGAATCCCTGGGCGATAGCGATATTGCGCAGCTTGCCCGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTAGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCACGGCtgttgt                                                                                              >  2:3920296/1‑140 (MQ=255)

                                          aCGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtg                                                                                             <  2:1970434/140‑1 (MQ=255)

                                             aaGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtgttg                                                                                          >  1:1761400/1‑140 (MQ=255)

                                                  ggTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCa                                                                                     <  1:939098/140‑1 (MQ=255)

                                                  ggTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCa                                                                                     <  1:5231065/140‑1 (MQ=255)

                                                    tGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCAtt                                                                                   >  2:3490967/1‑140 (MQ=255)

                                                        tCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCa                                                                               >  1:1498867/1‑140 (MQ=255)

                                                        tCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCa                                                                               >  1:1240631/1‑140 (MQ=255)

                                                        tCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCa                                                                               >  2:1238255/1‑140 (MQ=255)

                                                              ggcgatggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGCCGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACa                                                                         >  2:2585383/1‑140 (MQ=255)

                                                              ggcgatggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACa                                                                         <  1:2376555/140‑1 (MQ=255)

                                                                   tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGTTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa                                                                    <  1:4457680/140‑1 (MQ=255)

                                                                   tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTTTTGTTTCATTTGCAGGAACAGCGGa                                                                    <  2:4795495/140‑1 (MQ=255)

                                                                   tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa                                                                    <  1:1925520/140‑1 (MQ=255)

                                                                   tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa                                                                    <  1:3151837/140‑1 (MQ=255)

                                                                   tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa                                                                    <  1:2401675/140‑1 (MQ=255)

                                                                   tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa                                                                    <  1:754810/140‑1 (MQ=255)

                                                                        atatTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGcc                                                               <  1:2316729/140‑1 (MQ=255)

                                                                        atatTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGcc                                                               >  2:3342311/1‑140 (MQ=255)

                                                                              cgcAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTAc                                                         >  1:4862400/1‑140 (MQ=255)

                                                                                   cTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGc                                                    >  2:3079175/1‑140 (MQ=255)

                                                                                      gCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGt                                                 >  2:3060098/1‑140 (MQ=255)

                                                                                          ttgtCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                             <  1:4712761/139‑1 (MQ=255)

                                                                                          gtgtCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                             <  1:4311513/140‑1 (MQ=255)

                                                                                          gtgtCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                             <  1:1366804/140‑1 (MQ=255)

                                                                                          gtgtCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                             <  1:1396374/140‑1 (MQ=255)

                                                                                          gtgtCGGGGGCGCGTGAGATCAATACCTGACGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTCCCGGGCGGTTGag                                             <  2:2016413/140‑1 (MQ=255)

                                                                                          gggtCGGTGACGCGTGAGAGCAATCCCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGag                                             <  2:2606120/138‑1 (MQ=255)

                                                                                           tgtCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagc                                            >  2:3467120/1‑140 (MQ=255)

                                                                                           tgtCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagc                                            >  2:538005/1‑140 (MQ=255)

                                                                                             tCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGGCTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagcag                                          <  2:400561/140‑1 (MQ=255)

                                                                                             tCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagcag                                          >  1:2813282/1‑140 (MQ=255)

                                                                                             tCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagcag                                          >  1:2667138/1‑140 (MQ=255)

                                                                                             tCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagcag                                          >  1:4859957/1‑140 (MQ=255)

                                                                                             tCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagcag                                          >  2:452249/1‑140 (MQ=255)

                                                                                             tCGGGGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagcag                                          >  2:451703/1‑140 (MQ=255)

                                                                                               ggTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGcc                                        <  1:2844493/140‑1 (MQ=255)

                                                                                               ggTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGcc                                        <  1:1587710/140‑1 (MQ=255)

                                                                                               ggTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGcc                                        <  2:3777253/140‑1 (MQ=255)

                                                                                               ggTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGcc                                        <  1:3474568/140‑1 (MQ=255)

                                                                                                gTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCGGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCa                                       >  1:1013737/1‑140 (MQ=255)

                                                                                                    cgcgTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaa                                   <  2:146849/140‑1 (MQ=255)

                                                                                                     gcgTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaag                                  >  1:2145485/1‑140 (MQ=255)

                                                                                                      cgTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaaga                                 <  1:4030553/140‑1 (MQ=255)

                                                                                                        tGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAAc                               <  1:1659861/140‑1 (MQ=255)

                                                                                                        tGAGAGCAATACCTGATCCTGGTAGGGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAAc                               <  2:3538399/140‑1 (MQ=255)

                                                                                                           gagCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGa                            <  2:753513/140‑1 (MQ=255)

                                                                                                           gagCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGa                            <  1:451703/140‑1 (MQ=255)

                                                                                                            agCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGAc                           <  2:2691527/140‑1 (MQ=255)

                                                                                                            agCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGAc                           <  1:2068559/140‑1 (MQ=255)

                                                                                                            agCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGAc                           <  1:2227833/140‑1 (MQ=255)

                                                                                                            agCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGAc                           <  1:4255316/140‑1 (MQ=255)

                                                                                                            agCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGAc                           <  2:1898224/140‑1 (MQ=255)

                                                                                                               aaTACCTGGTGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGCACAGCGGGTCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGGACTGAccag                        >  2:3192565/1‑138 (MQ=255)

                                                                                                                aTACCTGATGCTGGTAGCGGCGACGGAGGACTTGTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGTGGTTGAGCAGCAAGGAAGAACGGACCGGa                       >  2:2842579/1‑140 (MQ=255)

                                                                                                                 tACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCCCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGAt                      <  2:4899555/140‑1 (MQ=255)

                                                                                                                  aCCTGCTGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATa                     <  2:3694913/140‑1 (MQ=255)

                                                                                                                   ccTGATGCTGGTAGCGGCGTCGCAGGACTTTTTTAACGCGGCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGAACGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAg                    <  1:566252/140‑1 (MQ=255)

                                                                                                                      gATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCgg                 <  1:3245339/140‑1 (MQ=255)

                                                                                                                      gATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCgg                 >  1:1110197/1‑140 (MQ=255)

                                                                                                                      gATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCgg                 >  2:610820/1‑140 (MQ=255)

                                                                                                                      gATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCgg                 <  1:5317230/140‑1 (MQ=255)

                                                                                                                      gATGCTGGTAGCAGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCgg                 >  2:5285862/1‑140 (MQ=255)

                                                                                                                       aTGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGc                >  2:2119370/1‑140 (MQ=255)

                                                                                                                       aTGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGc                <  1:4772781/140‑1 (MQ=255)

                                                                                                                       aTGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACACCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGAGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGc                <  2:4622736/140‑1 (MQ=255)

                                                                                                                        tGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCt               >  1:2024747/1‑140 (MQ=255)

                                                                                                                           tGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCGTTTGCAGGAAGAGCGGAGCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcat            <  2:2232168/140‑1 (MQ=255)

                                                                                                                            ggTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcatc           <  1:2704120/140‑1 (MQ=255)

                                                                                                                            ggTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcatc           >  2:3794754/1‑140 (MQ=255)

                                                                                                                             gTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcatca          >  2:4232230/1‑140 (MQ=255)

                                                                                                                             gTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcatca          >  1:499557/1‑140 (MQ=255)

                                                                                                                             gTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGATGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcatca          >  2:728457/1‑140 (MQ=255)

                                                                                                                                 cggcgACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGACCTGACCGGATAGCGGCTCATCACATc      >  1:4376419/1‑140 (MQ=255)

                                                                                                                                 cggcgACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATCACATc      >  2:465818/1‑140 (MQ=255)

                                                                                                                                  ggcgACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATCACATcc     >  2:2999707/1‑140 (MQ=255)

                                                                                                                                     gACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGCACTGACCGGATAGCGGCTCATCACATCCACt  <  2:4166503/140‑1 (MQ=255)

CCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATCACATCCACT  >  NZ_CP025268/2122067‑2122339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`