BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,224,824	A→G	C401R (TGT→CGT)	bglX ←	beta‑glucosidase BglX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,224,824	0	A	G	99.4%	1078.6 / NA	329	C401R (TGT→CGT)	bglX	beta‑glucosidase BglX
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (134/191); total (134/195)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 329 total aligned reads displayed.

TCCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCAT  >  NZ_CP025268/2224689‑2224959

 cccGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGtttt                                                                                                                                    >  2:3547836/1‑140 (MQ=255)

     gTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGACCAACCACCGCAATGGTGGCCGATTTTGTCAGCGGTAACGTTACGAGACGGTTTTTCAg                                                                                                                                >  1:2971991/1‑140 (MQ=255)

      tCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGATTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTCACGTTTCGAGACGGTCTTTCCGc                                                                                                                               >  2:3942290/1‑140 (MQ=255)

          tACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGGCACGTTTACTGTCCGCCAGTGGCCCCACCCCCGCCATGGTGGCCGCTTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAacc                                                                                                                           >  1:1152539/1‑139 (MQ=255)

              gTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCaa                                                                                                                       >  2:1412589/1‑140 (MQ=255)

               tCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCATGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCTGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAg                                                                                                                      >  1:4565553/1‑140 (MQ=255)

               tCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAg                                                                                                                      >  1:1654408/1‑140 (MQ=255)

               tCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAg                                                                                                                      >  1:2342031/1‑140 (MQ=255)

               tCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAg                                                                                                                      >  1:2828021/1‑140 (MQ=255)

               tCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAg                                                                                                                      >  1:3245528/1‑140 (MQ=255)

               tCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAg                                                                                                                      >  2:2415715/1‑140 (MQ=255)

                  cGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCtt                                                                                                                   >  1:2101876/1‑140 (MQ=255)

                        gATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgc                                                                                                             <  1:1044467/140‑1 (MQ=255)

                         aTCGGCAACACCGGCTGCGGACCAGCTGCCCCTCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGATTTTCAGCAACACACAGCTTTcgcgag                                                                                                            >  2:2366297/1‑138 (MQ=255)

                         aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                            >  1:4273652/1‑140 (MQ=255)

                         aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                            >  2:3351130/1‑140 (MQ=255)

                          tCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCgcgcgc                                                                                                           <  2:1004915/140‑1 (MQ=255)

                                acacCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTc                                                                                                     <  2:869155/140‑1 (MQ=255)

                                 cacCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCAAACCACCGCAATGGTGGCCGAGTTTTTCAGCGGGAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCa                                                                                                    >  2:4688982/1‑140 (MQ=255)

                                   ccGGCTGCGGACCAGCTGCCCATCCAGTCACATTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACCTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                  <  2:4060467/140‑1 (MQ=255)

                                   ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCACCCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTCCGCGCGCCACTTCAcg                                                                                                  <  1:2452507/140‑1 (MQ=255)

                                   ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                  <  2:2822307/140‑1 (MQ=255)

                                   ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                  <  1:4879044/140‑1 (MQ=255)

                                   ccGGCTGCCGACCAGCTGCGAATTACGTAACGTTTACTGTCCGACAGTGGCCCAACCACCGCAATGGGGGCCGAGTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                  <  2:3720966/140‑1 (MQ=255)

                                   ccGGCGGCGGACCATCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACCCCAAGCTTTCGCGCGCCACTTCAcg                                                                                                  <  2:5316228/140‑1 (MQ=255)

                                     ggCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcgcg                                                                                                <  1:1245706/140‑1 (MQ=255)

                                     ggCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcgcg                                                                                                <  1:330669/140‑1 (MQ=255)

                                        tGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCGGCGAGTGGCCCAACCACCGGAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGGGCGCCACTTCACGCGctt                                                                                             >  1:2779971/1‑140 (MQ=255)

                                          cGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGcttct                                                                                           <  1:1508965/140‑1 (MQ=255)

                                            gACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCttt                                                                                         >  2:1211462/1‑140 (MQ=255)

                                                    gCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCa                                                                                 >  2:49325/1‑140 (MQ=255)

                                                      ccATCACGTCACGTTTACTGTCCGCCAGTGGCCCACCCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAgg                                                                               <  2:3834138/140‑1 (MQ=255)

                                                      ccATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAgg                                                                               <  1:2744358/140‑1 (MQ=255)

                                                        aTCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAggcg                                                                             <  1:1609572/140‑1 (MQ=255)

                                                          cacgtcacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAggcggc                                                                           >  1:332935/1‑140 (MQ=255)

                                                            cgtcacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCtt                                                                         >  2:2509244/1‑140 (MQ=255)

                                                             gtcacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCttt                                                                        <  1:1352925/140‑1 (MQ=255)

                                                             gtcacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCttt                                                                        <  1:2753381/140‑1 (MQ=255)

                                                               cacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCg                                                                      >  2:2705295/1‑140 (MQ=255)

                                                                acgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGATTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCgg                                                                     <  2:50756/140‑1 (MQ=255)

                                                                  gtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCa                                                                   <  1:4369570/140‑1 (MQ=255)

                                                                  gtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCa                                                                   <  1:2042826/140‑1 (MQ=255)

                                                                       cTGTCCGCCAGTGGCCCAACCACCGCAATGGTGTCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGTGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGt                                                              <  2:2941067/140‑1 (MQ=255)

                                                                         gTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTAt                                                            >  1:2431397/1‑140 (MQ=255)

                                                                         gTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGGTTTTCAGCAACACCAAGCTTTCGCGCGCCCCTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTAt                                                            >  2:4153622/1‑140 (MQ=255)

                                                                            cGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCa                                                         >  1:627204/1‑140 (MQ=255)

                                                                            cGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCa                                                         >  2:571449/1‑140 (MQ=255)

                                                                            cGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGCCATTGGTATCCa                                                         <  1:725922/140‑1 (MQ=255)

                                                                             gCCAGTGGCCCACCCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCAc                                                        <  1:220287/140‑1 (MQ=255)

                                                                             gCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCAc                                                        <  2:3639012/140‑1 (MQ=255)

                                                                              ccAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCAcc                                                       <  1:2513143/140‑1 (MQ=255)

                                                                              ccAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCAcc                                                       <  2:2486822/140‑1 (MQ=255)

                                                                                 gcggCCCAACCACCGCAATGGTGGCCGCTTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCCACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCggg                                                    <  2:1700640/138‑1 (MQ=255)

                                                                                  tGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGt                                                   >  1:3663460/1‑140 (MQ=255)

                                                                                  tGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTCTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGt                                                   <  1:4458021/140‑1 (MQ=255)

                                                                                    gCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCa                                                 >  1:1910102/1‑140 (MQ=255)

                                                                                    gCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGCTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTTGGCATTGGTATCCACCGGGTCa                                                 >  2:4210146/1‑140 (MQ=255)

                                                                                      ccAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGa                                               <  1:2082406/140‑1 (MQ=255)

                                                                                         accaccGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTGAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGActc                                            >  2:2981757/1‑140 (MQ=255)

                                                                                         accaccGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGActc                                            >  2:3039922/1‑140 (MQ=255)

                                                                                         accaccGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGActc                                            >  1:3762493/1‑140 (MQ=255)

                                                                                            accGCACTGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACCCCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCttt                                         <  1:1697818/140‑1 (MQ=255)

                                                                                            accGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACGGGGTCAGACTGttt                                         >  1:3410764/1‑140 (MQ=255)

                                                                                            accGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCttt                                         <  1:1011191/140‑1 (MQ=255)

                                                                                              cGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCg                                       >  2:1480103/1‑140 (MQ=255)

                                                                                                cAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCgg                                                                     <  1:1070483/108‑1 (MQ=255)

                                                                                                 aaTGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGcc                                    <  1:2705574/140‑1 (MQ=255)

                                                                                                 aaTGGTAGCCGATTTTTCCAGCGGTAACGTTTCGAGACAGTTTTCCACCCACACCCCGCCTTCGCGCGCCACTCCACGCGCTTCTTTACGGTGCAGACGGCTTTCGGCATTGGTATCCACCTGGTCAGACTCTTTCGGct                                    <  2:41744/140‑2 (MQ=255)

                                                                                                    tgtggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCa                                 <  1:4712429/139‑1 (MQ=255)

                                                                                                    ggtggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCa                                 <  1:729587/140‑1 (MQ=255)

                                                                                                      tggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCaaa                               <  1:2646570/140‑1 (MQ=255)

                                                                                                      tggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCaaa                               >  1:5214560/1‑140 (MQ=255)

                                                                                                       ggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAAt                              <  2:4828752/140‑1 (MQ=255)

                                                                                                       ggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAAt                              <  1:4165712/140‑1 (MQ=255)

                                                                                                       ggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAAt                              <  2:5219348/140‑1 (MQ=255)

                                                                                                        gCCGATTTTTTCAGCGGTAACGTTTGGATACGGTATGGCAGCAACACTAAGCTTTCGCGCGCCACTTCACGCTCTTCTTTACGGTGCAGGCGGCTTTTGGGGTTGGCATCCACCGGGTGAGTGTCTTGCGGCCCCaaagg                             >  2:2262513/1‑138 (MQ=255)

                                                                                                        gCCGATTGTTTCAGCGGTAACGTTTCGAGACGGTTTGTCAGCAACACGAAGCTTTCGCGAGCCACTTCACGCGATTCTTGACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCATGCTCTTTCGGCCCCaaaag                             >  2:456996/1‑138 (MQ=255)

                                                                                                         ccGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATgg                            <  1:800920/140‑1 (MQ=255)

                                                                                                         ccGATTTTTTCAGCGGTAACGTTTCGAGACCGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTTCAGGCGGCTTTCGGCATTGGTATCCACCGCGTCAGACTCTTTCGGCCCCAAATgg                            <  2:1088844/140‑1 (MQ=255)

                                                                                                              tttttCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTa                       <  1:1765410/140‑1 (MQ=255)

                                                                                                                tttCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATg                     <  1:1399532/140‑1 (MQ=255)

                                                                                                                tttCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATg                     <  1:4118628/140‑1 (MQ=255)

                                                                                                                tttCACCGGTAACGTTGCGAGACTGTTTTTCAGCTCCACCACGCTTTCGCGCGCCACTTCACGCGCTTCTTTTCGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATg                     <  2:2898303/140‑1 (MQ=255)

                                                                                                                   cAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGt                  <  1:523002/140‑1 (MQ=255)

                                                                                                                     gCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCg                <  2:3683686/140‑1 (MQ=255)

                                                                                                                     gCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCg                <  1:5059032/140‑1 (MQ=255)

                                                                                                                      cGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGt               <  2:106881/140‑1 (MQ=255)

                                                                                                                          aaCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTaaa           <  2:4531061/140‑1 (MQ=255)

                                                                                                                          aaCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTaaa           <  2:75987/140‑1 (MQ=255)

                                                                                                                          aaCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTaaa           <  1:3975232/140‑1 (MQ=255)

                                                                                                                          aaCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTaaa           <  1:554320/140‑1 (MQ=255)

                                                                                                                          aaCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCCTTCCGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTaaa           <  1:2923411/140‑1 (MQ=255)

                                                                                                                           acctttCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAaac          <  1:3013654/137‑1 (MQ=255)

                                                                                                                           aCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAaac          <  2:644169/140‑1 (MQ=255)

                                                                                                                           aCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAaac          <  2:3263483/140‑1 (MQ=255)

                                                                                                                               ttCGAGCCAGTTTCTCAGCATCACCAAGCTTGCTGCCGCCACTTCACGCGCTTCTTTACGGGGCAGGCGGCTTTCGGCATTGGTATTCACCGGGTCAGTCTCTTTCGGCCCCAAATGGCTTTATGGGTCGTTAAACAAcc      <  2:817043/140‑1 (MQ=255)

                                                                                                                               ttCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAAcc      <  1:4217993/140‑1 (MQ=255)

                                                                                                                               ttCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAAcc      <  1:2604168/140‑1 (MQ=255)

                                                                                                                                 cGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAAcccc    <  1:5298333/140‑1 (MQ=255)

                                                                                                                                   agaCGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATCGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGCTAAACAACCCcat  >  2:4342725/1‑140 (MQ=255)

TCCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCAT  >  NZ_CP025268/2224689‑2224959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`