BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	247,291	A→G	250 (TAA→TAG)	yafL →	C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	247,291	0	A	G	99.6%	907.7 / ‑5.9	276	250 (TAA→TAG)	yafL	C40 family peptidase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (120/155); minor base T (0/1); total (120/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 276 total aligned reads displayed.

GTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑G‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NZ_CP025268/247154‑247423

gtgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                      <  1:1151592/140‑1 (MQ=255)

gtgtATTTGGGCGATGGGAAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                      <  1:4473630/140‑1 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                     >  1:4593151/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                     >  2:819093/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                     >  1:5112174/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                     >  1:2178872/1‑140 (MQ=255)

  gtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑g                                                                                                                                   >  1:5014702/1‑140 (MQ=255)

  gtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑g                                                                                                                                   >  1:3514903/1‑140 (MQ=255)

  gtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑g                                                                                                                                   >  1:2034624/1‑140 (MQ=255)

   tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGCTTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑gg                                                                                                                                  >  2:1807007/1‑139 (MQ=255)

   tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑gg                                                                                                                                  >  1:371428/1‑139 (MQ=255)

       tGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑ggataa                                                                                                                              <  1:2327594/140‑6 (MQ=255)

        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATCAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAg                                                                                                                             <  1:2941525/140‑1 (MQ=255)

        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGAGTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGATACGTTTTTGTAGGACTG‑ATAAg                                                                                                                             >  2:4540261/1‑140 (MQ=255)

         ggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAgg                                                                                                                            >  2:4662523/1‑140 (MQ=255)

         ggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAgg                                                                                                                            >  2:3674880/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCTACGTACCGGCGAAACCATTCGGATAAGCCGGTTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                        >  2:2664305/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                        >  2:3846066/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                        >  1:2601441/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                        >  1:264672/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                        >  2:1164558/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                        >  2:1211557/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                        >  2:1743680/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                        <  2:2561219/140‑1 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                        >  2:603194/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                        >  1:374912/1‑140 (MQ=255)

                ggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTAc                                                                                                                     >  2:3345783/1‑140 (MQ=255)

                ggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTAc                                                                                                                     >  1:3676533/1‑140 (MQ=255)

                    aTTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTAcgccg                                                                                                                 <  1:1807670/140‑1 (MQ=255)

                         tCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATcc                                                                                                            <  1:5313653/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:952949/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  2:72583/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:441816/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:2918864/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                          >  1:1291221/1‑140 (MQ=255)

                              tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                       <  1:112297/140‑1 (MQ=255)

                                  cACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGt                                                                                                   <  2:2962373/140‑1 (MQ=255)

                                   aCGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                  <  1:1376327/140‑1 (MQ=255)

                                   aCGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                  <  1:2869977/140‑1 (MQ=255)

                                   aCGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                  <  1:2069424/140‑1 (MQ=255)

                                     gTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTCTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                                                <  2:4899265/140‑1 (MQ=255)

                                       aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                              <  1:807444/140‑1 (MQ=255)

                                          ggCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGt                                                                                           <  1:3881570/140‑1 (MQ=255)

                                           gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTGTGGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTg                                                                                          >  2:291116/1‑140 (MQ=38)

                                            cGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGc                                                                                         >  1:1440116/1‑140 (MQ=255)

                                              aaaCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCt                                                                                       <  1:1870419/140‑1 (MQ=255)

                                              aaaCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCt                                                                                       <  1:685016/140‑1 (MQ=255)

                                                         aTAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCt                                                                            >  1:812621/1‑140 (MQ=255)

                                                           aaGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg                                                                          <  1:4532173/140‑1 (MQ=255)

                                                            aGCCGATTAGCCTAACCTTTCTGGCAGGACCTTTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACTG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGTAGGTGCCTGATGCGACGCTGgc                                                                         <  2:4419436/140‑1 (MQ=38)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:3621014/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:3407056/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:4295168/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:4229024/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:4689675/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  2:1556602/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGGTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  2:3910561/140‑1 (MQ=255)

                                                             gCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcg                                                                        <  1:3054031/140‑1 (MQ=255)

                                                              ccGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcgc                                                                       >  1:4148567/1‑140 (MQ=255)

                                                                gATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                     <  2:1399858/140‑1 (MQ=255)

                                                                gATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                     <  2:5154602/140‑1 (MQ=255)

                                                                 aTTAGCCGAACGTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAATAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTc                                                                    >  2:3808465/1‑140 (MQ=37)

                                                                 aTTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTc                                                                    >  2:1045055/1‑140 (MQ=255)

                                                                  ttAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCt                                                                   >  1:4798029/1‑140 (MQ=255)

                                                                    aGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtga                                                                 <  1:2499753/140‑3 (MQ=255)

                                                                    aGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTa                                                                 <  1:1012656/140‑1 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  2:4616137/140‑1 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  2:3137321/140‑1 (MQ=255)

                                                                        gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCTGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                             <  2:2286239/140‑1 (MQ=255)

                                                                        gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                             <  2:2633157/140‑1 (MQ=255)

                                                                        gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                             <  1:3279948/140‑1 (MQ=255)

                                                                              ttCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc                                                       >  1:5228146/1‑140 (MQ=255)

                                                                               tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACg                                                      >  1:2421554/1‑140 (MQ=255)

                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGa                                                     <  2:426665/140‑1 (MQ=255)

                                                                                 tGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAg                                                    <  2:2200255/140‑1 (MQ=255)

                                                                                 tGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAg                                                    <  2:4323502/140‑1 (MQ=255)

                                                                                    cAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGccc                                                 <  1:2213771/140‑1 (MQ=255)

                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                              <  2:4944117/93‑1 (MQ=37)

                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                               <  2:2996779/140‑1 (MQ=255)

                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                               >  1:3060054/1‑140 (MQ=255)

                                                                                        aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGa                                             >  2:2802498/1‑140 (MQ=255)

                                                                                                            aagATTTTGGGGGACGGGGCGATTTTGTATGGG‑GAATAAGGCGTTGACGCAGCACCCGACAGTTGTACGCAGGTGCCTGATGCGGCGCAGTCGAGTCTAATCATGCCTACGAGCCCGCGCATATTTGCCAGCGGCtttgc                         <  2:580699/138‑3 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:3452524/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:90991/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  1:4392286/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  1:4210353/140‑1 (MQ=255)

                                                                                                             ggATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTccc                        <  1:1953097/140‑1 (MQ=255)

                                                                                                               cttttGACGGAAGAGACGATTTTGTAGGACGG‑AGACGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGa                      <  2:2029703/139‑1 (MQ=25)

                                                                                                                ttttGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGat                     <  2:4806485/140‑1 (MQ=255)

                                                                                                                ttttGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGat                     <  2:3983403/140‑1 (MQ=255)

                                                                                                                ttttGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGat                     <  2:3541283/140‑1 (MQ=255)

                                                                                                                  ttGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGatat                   <  2:1487219/140‑1 (MQ=255)

                                                                                                                   tGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTAcg                                                                                                                    <  2:5273204/42‑1 (MQ=18)

                                                                                                                       ggAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaac              <  2:3059869/140‑1 (MQ=255)

                                                                                                                        gAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca             <  2:328232/140‑1 (MQ=255)

                                                                                                                        gAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca             >  1:488858/1‑140 (MQ=255)

                                                                                                                         aaGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaacaa            >  2:4559006/1‑140 (MQ=255)

                                                                                                                           gacaCGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCCTCCGGCAGTTCTCCGAAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAccc          <  2:1134207/137‑1 (MQ=21)

                                                                                                                                   tttGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc  <  1:122455/140‑1 (MQ=255)

                                                                                                                                   tttGTAGGACGG‑ATAAGCCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCGTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc  <  1:4059523/140‑1 (MQ=25)

GTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑G‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NZ_CP025268/247154‑247423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`