BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,595,629	A→G	119 (TAA→TAG)	yffB →	ArsC family reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,595,629	0	A	G	99.6%	741.6 / NA	222	119 (TAA→TAG)	yffB	ArsC family reductase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (132/89); total (132/90)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

CCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTGAACGCA  >  NZ_CP025268/2595493‑2595759

ccGATGCGGCCTCTGCGGCGGCATTCTTGACGGAAATGCCTGCCAGTATCAAACGTGCAGCGCTCTGCGTGCCCGTAAAGCCGATGCTGCTGGGAGCCAGTGAGTCCAGTTATCACCACATTGTCCACGAGTTGTAGTCt                                                                                                                                 >  2:3021406/1‑140 (MQ=255)

ccGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCt                                                                                                                                 >  1:4476719/1‑140 (MQ=255)

ccGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCt                                                                                                                                 >  2:3621900/1‑140 (MQ=255)

ccGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCt                                                                                                                                 <  2:2336759/140‑1 (MQ=255)

ccGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCt                                                                                                                                 <  2:2538569/140‑1 (MQ=255)

ccGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCt                                                                                                                                 >  1:1474076/1‑140 (MQ=255)

ccGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCt                                                                                                                                 >  1:2263604/1‑140 (MQ=255)

ccGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCt                                                                                                                                 <  2:3044279/140‑1 (MQ=255)

  gATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTAt                                                                                                                               >  2:1817046/1‑140 (MQ=255)

  gATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCGGGGGTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTAt                                                                                                                               >  2:1930230/1‑140 (MQ=255)

   aTGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATg                                                                                                                              >  1:1811512/1‑140 (MQ=255)

    tGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGt                                                                                                                             >  2:3943257/1‑140 (MQ=255)

        gCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTg                                                                                                                         <  2:3533670/140‑1 (MQ=255)

           tctGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGccc                                                                                                                      <  1:154099/140‑1 (MQ=255)

           tctGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGccc                                                                                                                      <  1:1013541/140‑1 (MQ=255)

            ctGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCg                                                                                                                     <  1:1112363/140‑1 (MQ=255)

              gcggcgGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCCGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGt                                                                                                                   >  1:217173/1‑140 (MQ=255)

               cggcggCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGtt                                                                                                                  >  2:2702039/1‑140 (MQ=255)

                ggcggcATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATCGCTCGGCGTGCACGGTAAGCCTATGCTGCTGGGTTTGAGTGATTCAAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTa                                                                                                                 <  2:4992818/140‑1 (MQ=255)

                 gcggcATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTAt                                                                                                                <  2:5058976/140‑1 (MQ=255)

                       ttAATGACTGAAATGCCTGCAATTATCAAACGTCCCTTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGGTTCAGTGATTCCCGTTATCAGCAATTTTTCCATGAGGTGTCGTCTCTGTCGTGCCCGGTTATTGAGCt                                                                                                          >  2:2412444/1‑140 (MQ=255)

                       ttAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCt                                                                                                          >  1:808186/1‑140 (MQ=255)

                       ttAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCt                                                                                                          <  2:1286454/140‑1 (MQ=255)

                         aaTGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCAGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGa                                                                                                        <  1:1368041/140‑1 (MQ=255)

                             aCTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGAcaca                                                                                                    <  2:615173/140‑1 (MQ=255)

                               tGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGTAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACacaac                                                                                                  >  2:5300229/1‑140 (MQ=255)

                               tGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACacaac                                                                                                  <  2:667029/140‑1 (MQ=255)

                               tGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACacaac                                                                                                  >  2:1517537/1‑140 (MQ=255)

                               tGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACacaac                                                                                                  <  2:4476719/140‑1 (MQ=255)

                                 aaaTGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGTGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGCGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAg                                                                                                >  2:3197431/1‑140 (MQ=255)

                                  aaTGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGc                                                                                               >  1:1767129/1‑140 (MQ=255)

                                        tGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTc                                                                                         >  1:1959918/1‑140 (MQ=255)

                                         gCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcg                                                                                        <  1:1209039/140‑1 (MQ=255)

                                         gCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTAGTGAGCTGACACAACAGCTTATTcg                                                                                        <  2:324648/140‑1 (MQ=255)

                                            aTTATCAATCGTACATTCCTCTACATACCCTGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGCTATCAGCAATTTGTCCAAGACTTGTAAGCTATGTCGTGCCCCGTTATTGAGCTGACACAACAGCCTATTcgccg                                                                                     <  2:4198364/140‑1 (MQ=255)

                                              tATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                                   <  1:3888/140‑1 (MQ=255)

                                              tATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTGTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                                   <  2:3822842/140‑1 (MQ=255)

                                              tATCAAACGGCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                                   <  2:1592524/140‑1 (MQ=255)

                                                    tcGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTccc                                                                             <  2:3576670/139‑1 (MQ=255)

                                                     cGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCt                                                                            <  1:5041494/140‑1 (MQ=255)

                                                       tCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGa                                                                          >  1:755768/1‑140 (MQ=255)

                                                        ccATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAg                                                                         >  1:1055173/1‑140 (MQ=255)

                                                         cATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGt                                                                        >  2:3366054/1‑140 (MQ=255)

                                                         cATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGt                                                                        >  2:142803/1‑140 (MQ=255)

                                                          aTTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTc                                                                       >  1:2492579/1‑140 (MQ=255)

                                                           ttGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTcc                                                                      <  1:3469042/140‑1 (MQ=255)

                                                              ctctGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtga                                                                   <  1:3983960/140‑1 (MQ=255)

                                                              ctctGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtga                                                                   >  1:4290691/1‑140 (MQ=255)

                                                                ctGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtgatg                                                                 <  1:3967874/140‑1 (MQ=255)

                                                                ctGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtgatg                                                                 >  1:5155217/1‑140 (MQ=255)

                                                                 tGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtgatga                                                                >  2:346028/1‑140 (MQ=255)

                                                                 tGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtgatga                                                                >  1:286059/1‑140 (MQ=255)

                                                                 tGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtgatga                                                                >  2:2160479/1‑140 (MQ=255)

                                                                   cGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGatgatg                                                              >  1:3489288/1‑140 (MQ=255)

                                                                    gTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGc                                                             >  1:2067435/1‑140 (MQ=255)

                                                                     tGCCCGGTAAGCCTATGCTGCTGGGTTTCCGTGTTTCCATTTATCTGCAAATTTTCCATGAGGTATAGTCTATGTCGTGCCCGGTTATTGAGCTGACACCACAGCTTATTCGCCGCCCTTCACTGAGTCCAGATCATGCa                                                            <  2:1560989/140‑1 (MQ=255)

                                                                     tGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCa                                                            <  2:1659933/140‑1 (MQ=255)

                                                                     tGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCa                                                            <  1:942099/140‑1 (MQ=255)

                                                                       cccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAgg                                                          >  2:4268449/1‑140 (MQ=255)

                                                                        gcGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGGGTAGTCTATGTCGTGCCCGGGTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGa                                                         >  1:3635107/2‑140 (MQ=255)

                                                                        ccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCGGGTGATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTTATGATGCAGGa                                                         >  2:4566637/1‑140 (MQ=255)

                                                                        ccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGa                                                         >  1:2773338/1‑140 (MQ=255)

                                                                        ccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGa                                                         >  2:4758864/1‑140 (MQ=255)

                                                                           gTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGc                                                      >  1:4888352/1‑140 (MQ=255)

                                                                            tAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGcc                                                     <  2:4769095/140‑1 (MQ=255)

                                                                            tAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGcc                                                     <  2:562427/140‑1 (MQ=255)

                                                                                    tgctgcTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTGCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCGGATGATGCAGGATGCCAGGCTttg                                             >  1:1628130/1‑140 (MQ=255)

                                                                                         ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGttgat                                        <  1:2726073/140‑1 (MQ=255)

                                                                                         ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGttgat                                        >  2:2085565/1‑140 (MQ=255)

                                                                                         ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGttgat                                        >  1:2881996/1‑140 (MQ=255)

                                                                                         ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGttgat                                        >  1:4809503/1‑140 (MQ=255)

                                                                                         ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGttgat                                        >  2:4308556/1‑140 (MQ=255)

                                                                                          tgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTTAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGttgatt                                       >  2:691070/1‑140 (MQ=255)

                                                                                          tgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCGAGGCTTTGttgatt                                       >  2:387403/1‑140 (MQ=255)

                                                                                          tgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGttgatt                                       >  2:1150242/1‑140 (MQ=255)

                                                                                           gggTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGttgattg                                      >  1:3087937/1‑140 (MQ=255)

                                                                                            ggTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGttgattga                                     <  1:1970603/140‑1 (MQ=255)

                                                                                              tttCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGCTGATGCAGGATGCCAGGCTTTGTTGATTGAAc                                   >  2:3718036/1‑140 (MQ=255)

                                                                                              tttCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAAc                                   >  1:3059031/1‑140 (MQ=255)

                                                                                                   gTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTg                              <  1:3841331/140‑1 (MQ=255)

                                                                                                        tCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGc                         >  1:1455823/1‑140 (MQ=255)

                                                                                                        tCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGc                         >  1:5341041/1‑140 (MQ=255)

                                                                                                        tCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGc                         >  2:4133098/1‑140 (MQ=255)

                                                                                                         ccAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCg                        <  2:291260/140‑1 (MQ=255)

                                                                                                           aGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGAt                      <  2:4361277/140‑1 (MQ=255)

                                                                                                           aGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGAt                      <  1:142803/140‑1 (MQ=255)

                                                                                                            gTTATCAGCACTTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACTTTTGCAGGCGATc                     >  1:1505556/1‑140 (MQ=255)

                                                                                                            gTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATc                     >  1:4170634/1‑140 (MQ=255)

                                                                                                                   gCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTa              <  2:285701/140‑1 (MQ=255)

                                                                                                                       tttttCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGGACGTTTGCAGGCGATCGGTTTTACCgg          >  1:1807457/1‑139 (MQ=255)

                                                                                                                         tttCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTg        <  1:3325117/140‑1 (MQ=255)

                                                                                                                         tttCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTg        <  1:3440212/140‑1 (MQ=255)

                                                                                                                         tttCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTg        <  1:231708/140‑1 (MQ=255)

                                                                                                                         tttCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTg        <  1:2080637/140‑1 (MQ=255)

                                                                                                                         tttCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTg        <  2:1414198/140‑1 (MQ=255)

                                                                                                                         tttCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTg        <  2:5221996/140‑1 (MQ=255)

                                                                                                                         tttCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTg        <  2:5222441/140‑1 (MQ=255)

                                                                                                                         tttCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTg        <  1:1922642/140‑1 (MQ=255)

                                                                                                                              aTGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTGAACGc   >  2:2097025/1‑140 (MQ=255)

                                                                                                                               tGAGGTGTAGTCTATGTCGTGCCCGGTTATTGCGCTGACACAACAGCTTATTCGCCGCCATTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTGAACGCa  <  1:1930230/140‑1 (MQ=255)

CCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTGAACGCA  >  NZ_CP025268/2595493‑2595759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`