BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,649,037	T→C	771 (TAA→TAG)	pbpC ←	peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,649,037	0	T	C	100.0%	552.8 / NA	164	771 (TAA→TAG)	pbpC	peptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (108/56); total (108/56)

Only 100 of 166 total aligned reads displayed.

TAAATTGTTCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCAC  >  NZ_CP025268/2648900‑2649172

tAAATTGTTCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGacta                                                                                                                                       >  2:3859254/1‑140 (MQ=255)

       ttCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATg                                                                                                                                >  2:5190376/1‑140 (MQ=255)

        tCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGa                                                                                                                               >  2:611305/1‑140 (MQ=255)

        tCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGa                                                                                                                               >  2:2059081/1‑140 (MQ=255)

        tCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGa                                                                                                                               >  1:2459371/1‑140 (MQ=255)

        tCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGa                                                                                                                               >  2:4871434/1‑140 (MQ=255)

        tCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTCTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGa                                                                                                                               >  1:2459457/1‑140 (MQ=255)

            ttgttgtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACaaa                                                                                                                           <  1:913431/140‑1 (MQ=255)

            ttgttgtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACaaa                                                                                                                           >  2:3550932/1‑140 (MQ=255)

            ttgttgtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACaaa                                                                                                                           >  2:3550922/1‑140 (MQ=255)

                 gtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAACTAAAATGAGTTTTAGCCACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCa                                                                                                                      >  1:2333779/1‑140 (MQ=255)

                 gtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGGTTTAGCAACAATCAGGGACAAAACAGACTATTACATGACAAATTTCa                                                                                                                      >  2:56939/1‑140 (MQ=255)

                      tACCAGCGTACGAGATGGCGCGGGTTATAATGAGGAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTGGTAAAATAAAATGAGGTTTAGCAACAATCAGGGACGAAACAGACTATTGCATGACAAAGGACACTGTc                                                                                                                 >  2:1762585/1‑140 (MQ=255)

                      tACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTc                                                                                                                 <  2:318131/140‑1 (MQ=255)

                      tACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTc                                                                                                                 >  1:2238628/1‑140 (MQ=255)

                      tACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTc                                                                                                                 >  1:1779632/1‑140 (MQ=255)

                      tACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTc                                                                                                                 >  2:1682307/1‑140 (MQ=255)

                           gCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATGTCACTGTCGCGAt                                                                                                            >  2:239663/1‑140 (MQ=255)

                           ccGTACGAGATGGCGCGGATTATAATTAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAt                                                                                                            <  2:5123088/139‑1 (MQ=255)

                            cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATTAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                           >  1:3084450/1‑140 (MQ=255)

                            cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                           >  1:3103556/1‑140 (MQ=255)

                            cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                           >  1:4680642/1‑140 (MQ=255)

                            cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                           >  1:4369622/1‑140 (MQ=255)

                            cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTAACTGTCGCGAtt                                                                                                           >  2:1557313/1‑140 (MQ=255)

                            cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATCGCATGACAAATTTCACTGTCGCGAtt                                                                                                           >  2:1950504/1‑140 (MQ=255)

                              tACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTg                                                                                                         >  1:831807/1‑140 (MQ=255)

                              tACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTg                                                                                                         >  1:1957482/1‑140 (MQ=255)

                              tACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTg                                                                                                         >  1:831808/1‑140 (MQ=255)

                                cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       >  1:3714161/1‑140 (MQ=255)

                                cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       >  1:2619057/1‑140 (MQ=255)

                                cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       >  1:1197737/1‑140 (MQ=255)

                                cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       >  1:138814/1‑140 (MQ=255)

                                cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       >  2:2715043/1‑140 (MQ=255)

                                cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGc                                                                                                        >  1:5157404/1‑139 (MQ=255)

                                 gagaTGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCCGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGccc                                                                                                      <  2:1402114/140‑1 (MQ=255)

                                 cagaTGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGccc                                                                                                      <  1:4730089/139‑1 (MQ=255)

                                     tGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACg                                                                                                  <  2:5263435/140‑1 (MQ=255)

                                      ggCGCGGATTATAATGAGCAACCGGGCCGTTGACTAGTTATGCCGGTAACATTTTTTTAAAATACAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATAACAAATTTCACTTTCGCGATTTGCCGCACGt                                                                                                 >  2:4701465/1‑140 (MQ=255)

                                      ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                 >  2:1383624/1‑140 (MQ=255)

                                      ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                 >  1:4678064/1‑140 (MQ=255)

                                      ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                 >  1:1690607/1‑140 (MQ=255)

                                      ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                 >  1:1690712/1‑140 (MQ=255)

                                      ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCACACgg                                                                                                 >  2:1083374/1‑139 (MQ=255)

                                        cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCATTGTCGCGATTTGCCCCACGTca                                                                                               >  1:3858104/1‑140 (MQ=255)

                                        cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTca                                                                                               >  1:2035325/1‑140 (MQ=255)

                                                   aTGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAg                                                                                    <  1:3550932/140‑1 (MQ=255)

                                                       gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAGATGAGGTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATTCATCACCAGCAAt                                                                                >  2:1136785/1‑140 (MQ=255)

                                                       gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                <  2:2823969/140‑1 (MQ=255)

                                                       gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                >  2:3732706/1‑140 (MQ=255)

                                                       gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                >  2:156164/1‑140 (MQ=255)

                                                         aaCAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTg                                                                              <  1:2175871/140‑1 (MQ=255)

                                                           cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGAt                                                                            >  1:4220035/1‑140 (MQ=255)

                                                           cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGAt                                                                            >  1:284993/1‑140 (MQ=255)

                                                             gggCCGTTGGCTATTGATGAAGGTAACAGGGTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACGATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATaa                                                                          <  2:3662578/140‑1 (MQ=255)

                                                             gggCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATaa                                                                          <  2:544412/140‑1 (MQ=255)

                                                             gggCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATaa                                                                          <  2:1713299/140‑1 (MQ=255)

                                                              ggCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAAt                                                                         <  1:611305/140‑1 (MQ=255)

                                                              ggCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAAt                                                                         <  1:3277081/140‑1 (MQ=255)

                                                               gCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTGGCGATTTGCCCCAAGTCATCCATCACCAGCAATTGATAATc                                                                        >  2:4253980/1‑140 (MQ=255)

                                                               gCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATc                                                                        <  1:3418064/140‑1 (MQ=255)

                                                                   ttGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCAGCAGCAATTGATAATCACCt                                                                    >  1:1338199/1‑140 (MQ=255)

                                                                   ttGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCt                                                                    >  1:282539/1‑140 (MQ=255)

                                                                   ttGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCt                                                                    >  2:1541618/1‑140 (MQ=255)

                                                                   ttGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCt                                                                    >  2:5029109/1‑140 (MQ=255)

                                                                     gACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCttt                                                                  >  1:3373655/1‑140 (MQ=255)

                                                                     gACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCttt                                                                  >  1:3883919/1‑140 (MQ=255)

                                                                       cTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTAt                                                                <  1:4852739/140‑1 (MQ=255)

                                                                          ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCg                                                             >  2:4008791/1‑140 (MQ=255)

                                                                           tgatgaAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCTACGTCATCCATCCCCATCAATTGATAATCACCTTTATCCGt                                                            >  2:78227/1‑140 (MQ=255)

                                                                            gatgaAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGCGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTc                                                           <  1:3984085/140‑1 (MQ=255)

                                                                                 aGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATg                                                      <  1:2083374/140‑1 (MQ=255)

                                                                                 aGGTAACATTTTCTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATg                                                      <  2:3766215/140‑1 (MQ=255)

                                                                                  ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAATAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACATCATCCATCACCAGCAATTGATAATCACCTTAATCCGTCAAATGc                                                     >  2:4623541/1‑140 (MQ=255)

                                                                                  ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACCAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGc                                                     >  2:2953674/1‑140 (MQ=255)

                                                                                  ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAGCAGACTATTGCATGACAAATTTCAATGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGc                                                     >  2:1035878/1‑140 (MQ=255)

                                                                                  ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGc                                                     >  2:280107/1‑140 (MQ=255)

                                                                                  ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGc                                                     >  2:3092326/1‑140 (MQ=255)

                                                                                  ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGc                                                     >  1:2714295/1‑140 (MQ=255)

                                                                                   gTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCa                                                    <  1:4410009/140‑1 (MQ=255)

                                                                                   gTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCa                                                    <  2:2127881/140‑1 (MQ=255)

                                                                                   gTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCa                                                    <  2:1356240/140‑1 (MQ=255)

                                                                                       cATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGt                                                >  2:3938294/1‑140 (MQ=255)

                                                                                       cATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGt                                                <  1:2128313/140‑1 (MQ=255)

                                                                                            ttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGt                                           >  1:1561286/1‑140 (MQ=255)

                                                                                            ttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGt                                           >  2:1694389/1‑140 (MQ=255)

                                                                                             tttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGtt                                          >  1:327014/1‑140 (MQ=255)

                                                                                                       aatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCACATGCAGGGTCACGTTGCGCCCGCGt                                <  2:4788506/140‑1 (MQ=255)

                                                                                                       aatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGt                                <  2:2456506/140‑1 (MQ=255)

                                                                                                       aatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGt                                <  2:2651116/140‑1 (MQ=255)

                                                                                                       aatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGt                                <  2:2650162/140‑1 (MQ=255)

                                                                                                        atGAGTTTTAGCAAAAATCAGGGACACAACAGACTATTGCATGACAAATTTCACTTTCGCGCTTTGCCCCACGTCATCCATCACCAGCCATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGtt                               <  1:5237098/140‑1 (MQ=255)

                                                                                                             ttttAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGtt                          >  1:3690322/1‑140 (MQ=255)

                                                                                                                 aGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACg                      <  1:1460054/140‑1 (MQ=255)

                                                                                                                     acaaTCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTc                  >  1:19383/1‑140 (MQ=255)

                                                                                                                     acaaTCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTc                  >  2:248711/1‑140 (MQ=255)

                                                                                                                             ggACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCa          >  1:4865936/1‑140 (MQ=255)

                                                                                                                             ggACAAAACAGACTATTGCATGACAAATTGCACTGTCGCGATTTCCCCCACGTCATCCCTCACCAGCCATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCa          <  2:1210868/140‑1 (MQ=255)

                                                                                                                               aCAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGa        <  2:3332856/140‑1 (MQ=255)

                                                                                                                                 aaaaCAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGaaa      >  1:5127727/1‑140 (MQ=255)

                                                                                                                                 aaaaCAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAAGGCAGGGTCACGTTTCGCCCGCGTTCAGTTAACGTTTCGCCATTCAGaaa      >  1:2060792/1‑140 (MQ=255)

TAAATTGTTCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCAC  >  NZ_CP025268/2648900‑2649172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`