BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,659,791	C→T	A198T (GCT→ACT)	pepB ←	aminopeptidase PepB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,659,791	0	C	T	99.7%	1365.2 / NA	368	A198T (GCT→ACT)	pepB	aminopeptidase PepB
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (169/197); total (170/198)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 368 total aligned reads displayed.

CAGCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGCTCACGC  >  NZ_CP025268/2659654‑2659927

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  1:5079040/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  1:4320722/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  2:4133434/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      <  2:1494482/140‑1 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  1:2850969/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  1:1714462/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  2:4088548/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  1:2795694/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  2:3303846/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  2:3626081/1‑140 (MQ=255)

     tCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAACGTGATACCt                                                                                                                                   >  1:4762073/1‑140 (MQ=255)

     tCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCt                                                                                                                                   >  1:1551275/1‑140 (MQ=255)

     tCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACACCACCGCTTCCCTAACCGCAGTCTGTGTGATGCTGTAGCCGCCGGCGTCAAAAGTGATACCt                                                                                                                                   >  2:2122632/1‑140 (MQ=255)

       aCGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCttt                                                                                                                                 >  1:753794/1‑140 (MQ=255)

            cTTGTTGGGTCCGCGCGTAATGTCAAATGACAGCGGCCCGGTCGCCGGTGCCGCGCGGCCCATGTCCGACTTCATCGAGTCCATAAGCGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAATGTGGCACCTTtaccta                                                                                                                            <  2:1647957/140‑1 (MQ=255)

               gTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCa                                                                                                                         <  1:2041369/140‑1 (MQ=255)

                ttCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAg                                                                                                                        >  2:3541895/1‑140 (MQ=255)

                 taaGTCAGGGCGTAGTGGCATGTGGCAGGGCCCCACGCACCATGACCGCGCCGCCGTTATCCGACTTAATGGAGTCCAGAAGCGCTGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAACGTAATACCTCTACCTACCAgg                                                                                                                       <  2:2705494/138‑1 (MQ=255)

                 tCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCTGTCCGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  2:2095664/140‑1 (MQ=255)

                 tCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  2:2378355/140‑1 (MQ=255)

                 tCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  2:3267434/140‑1 (MQ=255)

                 tCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  2:3772111/140‑1 (MQ=255)

                 tCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  1:1004032/140‑1 (MQ=255)

                     tCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCAcg                                                                                                                   >  1:4211894/1‑140 (MQ=255)

                       cgcgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCAcgcg                                                                                                                 <  1:4498444/140‑1 (MQ=255)

                       cgcgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCAcgcg                                                                                                                 <  1:1158714/140‑1 (MQ=255)

                         cgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCGGGCACGCGTa                                                                                                               >  2:3982416/1‑140 (MQ=255)

                         cgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTa                                                                                                               >  2:2000209/1‑140 (MQ=255)

                         cgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTa                                                                                                               >  1:375827/1‑140 (MQ=255)

                         cgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTa                                                                                                               <  1:1294398/140‑1 (MQ=255)

                               aTGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       >  1:3981006/1‑126 (MQ=255)

                               aTGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTgg                                                                                                         >  2:1936900/1‑140 (MQ=255)

                               aTGGCAAATGCCAGCGACCCGATAACCGTTGCCGCACCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTgg                                                                                                         <  2:2214023/140‑1 (MQ=255)

                                                 ccGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTATACTGGCGCTTCTTTCTCGCCAGt                                                                                       >  1:1445220/1‑140 (MQ=255)

                                                  cGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGtt                                                                                      <  2:806591/140‑1 (MQ=255)

                                                  cGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGtt                                                                                      <  2:3384192/140‑1 (MQ=255)

                                                   ggTACCCGTTGCCACGCCGCCCATGTCCGACTTCATCGAGTCCATAATCGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGTCGCTTCTTTATCGCCAGTTg                                                                                     <  2:4651157/140‑1 (MQ=255)

                                                   ggTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTg                                                                                     <  1:5226633/140‑1 (MQ=255)

                                                             gccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATc                                                                           <  1:5342018/140‑1 (MQ=255)

                                                             gccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATc                                                                           >  2:5227068/1‑140 (MQ=255)

                                                                   ccgccCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgcgc                                                                     >  1:2409234/1‑140 (MQ=255)

                                                                   ccgccCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgcgc                                                                     >  2:3796391/1‑140 (MQ=255)

                                                                    cgccCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGcc                                                                    <  1:2277770/140‑1 (MQ=255)

                                                                    cgccCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGcc                                                                    >  2:1584820/1‑140 (MQ=255)

                                                                      cccATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAg                                                                  <  1:1765885/140‑1 (MQ=255)

                                                                      cccATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAg                                                                  <  2:4972810/140‑1 (MQ=255)

                                                                           gTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATa                                                             >  2:1128041/1‑140 (MQ=255)

                                                                             ccGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATAcc                                                           <  1:793720/140‑1 (MQ=255)

                                                                                aCTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGga                                                        >  1:248476/1‑140 (MQ=255)

                                                                                aCTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGga                                                        >  1:908716/1‑140 (MQ=255)

                                                                                aCTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGga                                                        >  1:4413315/1‑140 (MQ=255)

                                                                                aCTTCATCGAGTCCATAAACGCAATCCGTTAGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTAGCACGCACGCGTACACTGCCGTTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGga                                                        >  1:3194361/1‑140 (MQ=255)

                                                                                  ttCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                                      <  2:2460923/140‑1 (MQ=255)

                                                                                     aTCGAGTCCATAAACGCAGTCTGTTTGATGCTGTTGCCGCCGGAGTCAAAGGTGATACCTTTACCTACCAGTCACGCGTACACTGGCGCTTCTTTATCGCCCGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAAca                                                   >  2:4583730/1‑139 (MQ=255)

                                                                                     aTCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACg                                                   >  2:4816510/1‑140 (MQ=255)

                                                                                     aTCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACg                                                   >  2:341644/1‑140 (MQ=255)

                                                                                          gTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTg                                              <  2:3081870/140‑1 (MQ=255)

                                                                                            ccATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGaa                                            <  2:5288562/140‑1 (MQ=255)

                                                                                              aTAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAAcc                                          <  2:4736874/140‑1 (MQ=255)

                                                                                              aTAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAAGCCAGCGCCAGCAATACCGGAGAACGTTCTGAAcc                                          <  2:1000450/140‑1 (MQ=255)

                                                                                              aTAAACGCAGTCTGTTTGATGCTATAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAAcc                                          <  1:4899437/140‑1 (MQ=255)

                                                                                                   cGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTc                                     <  1:4133621/140‑1 (MQ=255)

                                                                                                    gCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTcc                                    >  1:2329940/1‑140 (MQ=255)

                                                                                                     cAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCg                                   >  1:2667857/1‑140 (MQ=255)

                                                                                                      aGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGa                                  <  2:753794/140‑1 (MQ=255)

                                                                                                      aGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGa                                  <  1:3541381/140‑1 (MQ=255)

                                                                                                      aGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGa                                  <  2:4404063/140‑1 (MQ=255)

                                                                                                       gTCTGTTTGATGCTGTAGCCGCCGGTGTCAAAAGTGATTCCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGAc                                 >  2:430322/1‑140 (MQ=255)

                                                                                                       gTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGAc                                 <  2:2968524/140‑1 (MQ=255)

                                                                                                       gTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGAc                                 >  2:548830/1‑140 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  1:2177382/140‑1 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  1:3322188/140‑1 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  1:1387476/140‑1 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  1:3626081/140‑1 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  1:3842904/140‑1 (MQ=255)

                                                                                                        ccTGATTGCTGCTGTAGCCGCCGGCGTCAAAAGTGATACCTTTACCTCCCAGGCACGAGTACACTGGCGCATCTTTATCGCCAGTTGGGTTGTACTCCAGCGCCAGCAATACCGGAGAACCTTCTGAGCCGCGTCCGACt                                <  2:418381/139‑1 (MQ=255)

                                                                                                             ttGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTCCCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                           <  2:2612354/140‑1 (MQ=255)

                                                                                                             ttGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                           <  1:1860299/140‑1 (MQ=255)

                                                                                                             ttGATGCTGTAGCCGCCGGAGACAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                           <  2:716398/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTGCCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  2:3586350/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                           <  2:1362272/137‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  1:2996010/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  1:2584591/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  1:4262979/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTGGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        >  1:3277377/1‑140 (MQ=255)

                                                                                                                aTGCTGGAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCCGGCACGCGTACACTGGCGCTTCTTTATCGCCAATTGGGTTGTACTCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  2:280999/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  1:5023467/140‑1 (MQ=255)

                                                                                                                  ggtGTAGCCGCAGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcc                      <  2:937376/138‑1 (MQ=255)

                                                                                                                  gCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcc                      <  2:5098623/140‑1 (MQ=255)

                                                                                                                  gCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcc                      <  2:1218699/140‑1 (MQ=255)

                                                                                                                      tAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCat                  >  2:27183/1‑140 (MQ=255)

                                                                                                                      tAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCat                  >  1:569618/1‑140 (MQ=255)

                                                                                                                      tAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGGTGTAATCCAGCGCCAGCAATACCGGAGGACGTGCGGAACCGCGCCCGACTGTGTGCAGCACCat                  >  2:3060730/1‑140 (MQ=255)

                                                                                                                       aGCCGTCGGAGTCAAAAGTGCTACCTTTACCAACCAGGAACGCCTACCCATACCCATATTTATCGCCAGTAGGGTTGTTACCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCata                 <  2:182378/140‑1 (MQ=255)

                                                                                                                       aGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCata                 <  1:3478515/140‑1 (MQ=255)

                                                                                                                           gccgGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAAc             <  1:4542819/140‑1 (MQ=255)

                                                                                                                             cgGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGACCCGCGTCCGACTGTGTGCAGCCCCATATAACCt           >  2:61432/1‑140 (MQ=255)

                                                                                                                                agtttaaaGTGATACCTTTACCTACCATGCACGCGTACACTGGCGCTTTTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGc        <  2:1897377/135‑1 (MQ=255)

                                                                                                                                aGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGc        >  1:4808359/1‑140 (MQ=255)

                                                                                                                                      aaGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGCTCACGc  <  1:1987742/140‑1 (MQ=255)

CAGCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGCTCACGC  >  NZ_CP025268/2659654‑2659927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`