BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,746,986	A→G	N194D (AAT→GAT)	dgcN →	diguanylate cyclase DgcN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,746,986	0	A	G	99.7%	1065.8 / NA	323	N194D (AAT→GAT)	dgcN	diguanylate cyclase DgcN
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (185/137); minor base T (0/1); total (185/138)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 323 total aligned reads displayed.

GCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTA  >  NZ_CP025268/2746849‑2747120

  gACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGt                                                                                                                                    <  1:1179136/140‑1 (MQ=255)

  gACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGt                                                                                                                                    <  1:1014461/140‑1 (MQ=255)

  gACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGt                                                                                                                                    <  2:3521267/140‑1 (MQ=255)

  gACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGt                                                                                                                                    <  2:1689146/140‑1 (MQ=255)

  gACAGTTCAATCAGCCATTTTATCTGGTTTTCACTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGt                                                                                                                                    <  2:4445438/140‑1 (MQ=255)

  gACAGTTCAATCAGCCATTTTATCTGGTTTCCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGt                                                                                                                                    <  2:1590004/140‑1 (MQ=255)

       ttCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTAc                                                                                                                               >  2:4796502/1‑140 (MQ=255)

        tCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACa                                                                                                                              <  2:3235430/140‑1 (MQ=255)

         cAATCATCTATTTTATCTGGTTTTCGCTCGCCGTACTGACTGTTTGTAGTCTGCTGGCATCAGTCTTCGCAATTCCCTTCACCTGCCATTTGCACAATTGCCTTGTAGATTCGCTGAAAAATATCATCGATGTCGTACat                                                                                                                             <  2:2229627/140‑1 (MQ=255)

         cAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACat                                                                                                                             <  1:2916276/140‑1 (MQ=255)

            tCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACatgat                                                                                                                          >  2:4243061/1‑140 (MQ=255)

            tCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTACAAGCGCTGAAAAATATCACCGATGTCGTACatgat                                                                                                                          >  2:4952263/1‑140 (MQ=255)

               gCCATTGTATCTGGTTTTCGCTCGCCGTACTTACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGAtgtt                                                                                                                       >  2:36667/1‑139 (MQ=255)

                ccATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGc                                                                                                                      >  2:4602124/1‑140 (MQ=255)

                            gTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCa                                                                                                          >  1:491783/1‑140 (MQ=255)

                             ttttCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCaa                                                                                                         >  2:2139363/1‑140 (MQ=255)

                                 cgctcgcCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAAtttt                                                                                                     >  1:3753000/1‑140 (MQ=255)

                                            cTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGt                                                                                          <  1:2672413/140‑1 (MQ=255)

                                               aCCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTc                                                                                       >  2:1850998/1‑140 (MQ=255)

                                                ccGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCg                                                                                      <  1:907642/140‑1 (MQ=255)

                                                ccGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCg                                                                                      >  1:3860163/1‑140 (MQ=255)

                                                    ttGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGGTGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGgaag                                                                                  >  2:2015134/1‑140 (MQ=255)

                                                    ttGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGgaag                                                                                  >  2:1228481/1‑140 (MQ=255)

                                                    ttGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGgaag                                                                                  >  2:2938247/1‑140 (MQ=255)

                                                    ttGTATTCTGATGGCATCAGGCATCGCAATTACCCTCACCCGACATTTGCACAACGGCCTGATAGAAGCGCTGAAAAATATCACCGATGTCGTAAATGATGTGCGTTCCAACCGCAATTTTTCCCGACTAGTTTCGgaag                                                                                  >  2:4687548/1‑140 (MQ=255)

                                                             gctgGCATCAGGCATCGCAATTACCCTCTCCCGCCATTTGAATATTGGCCTGCTAGAAGCGCTGAAAAATATCGCCTATGTCGTACATGATGTGCGTTCCATCCGCGATTTTTCCCGACGAGTGTCGGAAGAACGTATCg                                                                         <  2:3031618/140‑1 (MQ=255)

                                                             gctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCg                                                                         <  2:1990490/140‑1 (MQ=255)

                                                             cctgGCATCAGGCATCGCAATTACCCTCTCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCg                                                                         <  1:4136441/139‑1 (MQ=255)

                                                              ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAATAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  1:4236090/1‑140 (MQ=255)

                                                              ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAATAACGTATCGc                                                                        >  2:4116174/1‑140 (MQ=255)

                                                              ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  2:846170/1‑140 (MQ=255)

                                                              ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  1:5252456/1‑140 (MQ=255)

                                                              ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  1:51871/1‑140 (MQ=255)

                                                              ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  1:4811436/1‑140 (MQ=255)

                                                              ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  1:138608/1‑140 (MQ=255)

                                                              ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  1:1668806/1‑140 (MQ=255)

                                                              ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  1:3594406/1‑140 (MQ=255)

                                                              ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        <  1:2032413/140‑1 (MQ=255)

                                                              ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATAATGTGCGTTCCAACCGCAATTTTTCCCCACGAGTTTCCGAAGAACGTATCGc                                                                        >  2:2853941/1‑140 (MQ=255)

                                                               tgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCt                                                                       <  1:1552994/140‑1 (MQ=255)

                                                               tgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCAAAATGGCCTGGTAGCAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCt                                                                       <  2:1123490/140‑1 (MQ=255)

                                                                   aTCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGt                                                                   >  1:3953604/1‑140 (MQ=255)

                                                                   aTCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGt                                                                   >  2:1460835/1‑140 (MQ=255)

                                                                     cAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGttt                                                                 <  2:5029936/140‑1 (MQ=255)

                                                                     cAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGttt                                                                 <  1:508527/140‑1 (MQ=255)

                                                                      aGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTc                                                                >  1:3349461/1‑140 (MQ=255)

                                                                        gCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCAc                                                              >  1:2207962/1‑140 (MQ=255)

                                                                           tCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGc                                                           >  1:1872743/1‑140 (MQ=255)

                                                                           tCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGc                                                           >  1:1962322/1‑140 (MQ=255)

                                                                           tCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGc                                                           >  2:1651296/1‑140 (MQ=255)

                                                                           tCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGc                                                           >  1:1704992/1‑140 (MQ=255)

                                                                           tCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGAAGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCAccac                                                           >  2:3158795/1‑138 (MQ=255)

                                                                            cGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGGAGAACGTATCGCTGAGTTTCACCGCt                                                          >  2:1893218/1‑140 (MQ=255)

                                                                            cGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCt                                                          >  1:833349/1‑140 (MQ=255)

                                                                             gCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGATGAGTTTCACCGCtt                                                         >  1:679638/1‑140 (MQ=255)

                                                                                    cccTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCtctc                                                  <  2:3417506/140‑1 (MQ=255)

                                                                                    cccTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCtctc                                                  <  2:730895/140‑1 (MQ=255)

                                                                                    cccTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCtctc                                                  <  2:4945756/140‑1 (MQ=255)

                                                                                         aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACtt                                             <  1:2010987/140‑1 (MQ=255)

                                                                                         aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACtt                                             >  2:1363570/1‑140 (MQ=255)

                                                                                         aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACtt                                             >  2:5284043/1‑140 (MQ=255)

                                                                                           ccGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCa                                           >  1:450806/1‑140 (MQ=255)

                                                                                           ccGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCa                                           >  2:4400480/1‑140 (MQ=255)

                                                                                                aTTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGt                                      <  1:1802710/140‑1 (MQ=255)

                                                                                                   tGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctg                                   >  1:2103479/1‑140 (MQ=255)

                                                                                                     cacaATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctgct                                 >  2:2036902/1‑140 (MQ=255)

                                                                                                       caATGGCCTGGTAGAAGCGCTGCAAAATATCACCGATGTCGTACATGATGTGCGCTCCAACCGCAATTTTTCCCGACGAGTTTCCGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTgg                               >  2:3885172/1‑140 (MQ=255)

                                                                                                        aaTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              <  2:1490482/140‑1 (MQ=255)

                                                                                                        aaTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              <  2:2810425/140‑1 (MQ=255)

                                                                                                        aaTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              <  1:2771164/140‑1 (MQ=255)

                                                                                                        aaTGGCCTGGGAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCGGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              <  2:2490831/140‑1 (MQ=255)

                                                                                                        aaTCGCCTGGTAGAAGTGCTGAAAAATATCACCGATGTCGTAAATGATGTGCGTTCCACCCGAAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGGTTCGCTCTCGACTTCAACAGTGCGCTGGa                              <  2:4019293/140‑1 (MQ=255)

                                                                                                         aTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGAt                             >  1:2387933/1‑140 (MQ=255)

                                                                                                         aTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGAt                             >  1:3078767/1‑140 (MQ=255)

                                                                                                         aTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGAt                             >  1:5075363/1‑140 (MQ=255)

                                                                                                         aTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGAt                             >  2:4154384/1‑140 (MQ=255)

                                                                                                         aTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTGTTCCCGACGAGGTTCGGAAGAACGTATCGCTGAGTTTCACGGCTTCGCTCTCGACTTCAAGAGTCTGCTGGAt                             >  2:4761917/1‑140 (MQ=255)

                                                                                                          tGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTTGCTCTCGACTTCAACAGGCTGCTGGATg                            >  2:640452/1‑140 (MQ=255)

                                                                                                          tGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGAGGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCAGCGGTTCGCTCTAGACTTCAAGAGTCGGCGGGATg                            >  2:179428/1‑140 (MQ=255)

                                                                                                                  tAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGaa                    >  2:3287641/1‑140 (MQ=255)

                                                                                                                      aGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGt                >  2:2594848/1‑140 (MQ=255)

                                                                                                                      aGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGt                >  1:1124521/1‑140 (MQ=255)

                                                                                                                      aGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGt                >  2:4876670/1‑140 (MQ=255)

                                                                                                                      aGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGt                >  2:379212/1‑140 (MQ=255)

                                                                                                                       gcgcTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAACTGGAAGAGTg               >  2:3980956/1‑140 (MQ=255)

                                                                                                                       gcgcTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTg               >  1:4638713/1‑140 (MQ=255)

                                                                                                                        cgcTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTgg              <  2:4646106/140‑1 (MQ=255)

                                                                                                                          gtGAAAAATACCCCCGATGTCGTACATGATGTGCGTTCCCACCGCAATTTTTCCCGACGAGTTTCGGAATAACGTACCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCa            <  2:5175512/139‑1 (MQ=255)

                                                                                                                          cTGAAAAATATCACCGATGGCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCa            <  1:3274186/140‑1 (MQ=255)

                                                                                                                           tGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAg           >  1:343751/1‑140 (MQ=255)

                                                                                                                            gAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGc          <  1:4606792/140‑1 (MQ=255)

                                                                                                                            gAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGc          <  1:287185/140‑1 (MQ=255)

                                                                                                                             aaaaaTATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCt         >  1:2405644/1‑140 (MQ=255)

                                                                                                                              aaaaTATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCtt        <  1:3614300/140‑1 (MQ=255)

                                                                                                                                    tCACCGATGTCGTACATGCTGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTa  <  2:1436264/140‑1 (MQ=255)

                                                                                                                                    tCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTa  <  2:1378382/140‑1 (MQ=255)

                                                                                                                                    tCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTa  <  2:3454940/140‑1 (MQ=255)

                                                                                                                                    tCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTa  <  1:1510555/140‑1 (MQ=255)

                                                                                                                                    tCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTa  <  2:2322454/140‑1 (MQ=255)

                                                                                                                                    tCACCGATGTCGTACATGATGGGCGTTCCAACCGCAAGTTTTCCCGAAGAGTTTCCGAGGAACGGATCGCTGCGTCTCACCGCTTCCCTCTCGACTTCAACAGTCTGCCGGCTGAAATGAACGAGTGGCAGCTTCGTTTa  <  2:1996110/140‑1 (MQ=255)

GCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTA  >  NZ_CP025268/2746849‑2747120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`