BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	306,029	C→T	V786M (GTG→ATG)	ecpC ←	fimbrial usher EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	306,029	0	C	T	99.3%	1105.5 / NA	299	V786M (GTG→ATG)	ecpC	fimbrial usher EcpC
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (142/154); minor base G (0/2); total (143/156)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

CGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCA  >  NZ_CP025268/305892‑306165

                  gCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGt                                                                                                                      >  1:3595834/1‑140 (MQ=255)

                  gCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGt                                                                                                                      >  2:3389577/1‑140 (MQ=255)

                  gCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGt                                                                                                                      >  2:2137212/1‑140 (MQ=255)

                      cTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTc                                                                                                                  >  1:1158283/1‑140 (MQ=255)

                      cTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTc                                                                                                                  >  1:2846393/1‑140 (MQ=255)

                       tGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCa                                                                                                                 >  2:1144625/1‑140 (MQ=255)

                        gCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCAt                                                                                                                <  1:3835007/140‑1 (MQ=255)

                            aCCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggt                                                                                                            >  2:270434/1‑140 (MQ=255)

                                 ataaCCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAACTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcggg                                                                                                       >  2:497230/4‑140 (MQ=255)

                                 aTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcggg                                                                                                       >  1:2542361/1‑140 (MQ=255)

                                 aTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcggg                                                                                                       >  2:873917/1‑140 (MQ=255)

                                   cACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggtt                                                                                                     <  2:2231527/140‑1 (MQ=255)

                                   cACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggtt                                                                                                     >  2:3064048/1‑140 (MQ=255)

                                    aCCGACCTAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTCTTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggtgc                                                                                                    <  2:151962/140‑3 (MQ=255)

                                    aCCGACCCAGACGGCAGCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggttc                                                                                                    <  1:2970579/140‑1 (MQ=255)

                                    aCCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggttc                                                                                                    <  1:4598387/140‑1 (MQ=255)

                                    aCCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggttc                                                                                                    <  1:4187091/140‑1 (MQ=255)

                                     ccGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggttcg                                                                                                   <  2:121054/140‑1 (MQ=255)

                                       gACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGc                                                                                                 <  2:1474436/140‑1 (MQ=255)

                                       gACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGGCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGc                                                                                                 <  2:1006267/140‑1 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTGTTATCCATGTACATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  1:2056608/1‑140 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  1:1341822/1‑140 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  1:4774165/1‑140 (MQ=255)

                                           cAGCCGGCACCGCGCGCCTCGTTGAGTTCCAGGGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGat                                                                                             <  2:843059/140‑1 (MQ=255)

                                           cAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGat                                                                                             >  1:186688/1‑140 (MQ=255)

                                           cAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGAAGGTCTTATGGCCACTGTAGCGAACATCGATACTGTGGTATTTCTTATCCATGTCAATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGat                                                                                             >  2:1303872/1‑140 (MQ=255)

                                           cAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCAACTCGCAGGTTTTATTTACACTGTAGCGAAAATCGATAGTGGTGTATTCCTTATCCTTGGCCATGACAATCTCGCCTTTTTCATCGGTTCGGGTTCGGCCGat                                                                                             <  2:2831762/140‑1 (MQ=255)

                                            aGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGata                                                                                            <  2:2027815/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  2:2779287/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  2:1407964/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:5059541/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           >  1:3087611/1‑140 (MQ=255)

                                               cGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATgg                                                                                         <  1:1634109/140‑1 (MQ=255)

                                                  cACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttg                                                                                      >  2:1982872/1‑140 (MQ=255)

                                                  cACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttg                                                                                      <  1:3869860/140‑1 (MQ=255)

                                                  cACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttg                                                                                      >  1:21725/1‑140 (MQ=255)

                                                  cACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttg                                                                                      >  2:225704/1‑140 (MQ=255)

                                                   aCCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttgt                                                                                     >  2:5044113/1‑140 (MQ=255)

                                                   aCCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttgt                                                                                     >  1:3476526/1‑140 (MQ=255)

                                                   aCCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttgt                                                                                     >  2:5206780/1‑140 (MQ=255)

                                                   aCCGCGCGCCTGGTTGAGATCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGGGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCCGCCGATATGgttgt                                                                                     >  2:3655189/1‑140 (MQ=255)

                                                    ccGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACACGCCGTTTTCATCGGTGCGGGTTCGGCCGATATGgttgtt                                                                                    >  1:4903030/1‑140 (MQ=255)

                                                      gcgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTaa                                                                                  >  2:905291/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  2:4424743/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGAGAGTGGGGTATTTCTTATCCATGGCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  2:2609914/1‑140 (MQ=255)

                                                       cgcgcCTGGATGAGTTCCTGAGGCACTTCGACGGATTTCCAGCCACTGTAGCCAAAATCGCAAGTGGCGTTTTTCTTAACCATATCAATGTCAAGCTCGCCGTTTTCATCGGTTCGTGTAAGTCCGATATGTTTGTTact                                                                                 >  2:1842393/1‑138 (MQ=255)

                                                         cgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATcc                                                                               <  2:2426017/140‑1 (MQ=255)

                                                         cgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATcc                                                                               <  1:1838203/140‑1 (MQ=255)

                                                            cTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTg                                                                            >  2:758051/1‑140 (MQ=255)

                                                              ggTTGGGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  2:3835003/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAACCTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  1:4522304/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  1:2459092/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  2:4803264/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  1:492365/140‑1 (MQ=255)

                                                              ggTTCAGTTCCGCAGCCACTCCGCAGGTTTTATTGCAACTGCAGGGAAAATCGATCGTGCGGTATTTCTTATCCATGTCCATGACAATCTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  2:2796836/140‑1 (MQ=255)

                                                                    gTTCCAGAGCCACTTCGCAGGTCTTAATGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATTTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGTTAGGGTTGTTAATCCGTGCGCTAccc                                                                    >  2:5318607/1‑140 (MQ=255)

                                                                     ttCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCca                                                                   <  2:2535006/140‑1 (MQ=255)

                                                                      tCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcag                                                                  <  1:686818/140‑1 (MQ=255)

                                                                      tCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcag                                                                  >  2:3144205/1‑140 (MQ=255)

                                                                      tCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcag                                                                  <  1:5249423/140‑1 (MQ=255)

                                                                       ccAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagc                                                                 <  2:667630/140‑1 (MQ=255)

                                                                        cAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATTGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagca                                                                <  1:4688218/140‑1 (MQ=255)

                                                                        cAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagca                                                                <  1:1417751/140‑1 (MQ=255)

                                                                        cAGAGCCACTTCGCAGGTGTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTGTTTCTTGTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagca                                                                >  2:1054750/1‑140 (MQ=255)

                                                                          gagCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCTATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAgt                                                              >  2:2988857/1‑140 (MQ=255)

                                                                          gagCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTTGGGTTCGGCCGATATGGTTGTTAATCCGTTCGTTAGCCAGCAgt                                                              >  2:5169821/1‑140 (MQ=255)

                                                                          gagCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGGATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGGTGTTAATCCGTGCGTTAGCcagcagg                                                              >  2:444807/1‑139 (MQ=255)

                                                                               aCTTCGCAGGTTTTATTGCCACTGTAGTGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCGACGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGAAGTGTGcc                                                         >  2:3908657/1‑140 (MQ=255)

                                                                               aCTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGGATTTCTTATCCATGTTCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGcc                                                         >  2:4907661/1‑140 (MQ=255)

                                                                               aCTTCGCAGGTGTTATTGCCACTGTATCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGcc                                                         <  2:4590032/140‑1 (MQ=255)

                                                                                cTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCg                                                        >  1:3375560/1‑140 (MQ=255)

                                                                                        gTTTTATTGCCACTGTAGCGCAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCAGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGc                                                <  1:4463551/140‑1 (MQ=255)

                                                                                             cttGCAACTTTAGCGAAAAAGCATAGTGGGGTTTTTCTTATCAATCTCCATCACATTCTCGCCGTTTTCACCGGTTCTCTTTCAGCCGATATAGTTCTTCATCCGTGCGTTCGCCAGCAGTGTGCCGTCTTCAGCACGGa                                           <  2:4268349/139‑1 (MQ=255)

                                                                                             aTTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGa                                           <  1:1446956/140‑1 (MQ=255)

                                                                                              ttGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGAt                                          <  1:107705/140‑1 (MQ=255)

                                                                                               tGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATa                                         <  2:3199055/140‑1 (MQ=255)

                                                                                               tGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATa                                         <  1:225704/140‑1 (MQ=255)

                                                                                               tGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATa                                         >  1:5318732/1‑140 (MQ=255)

                                                                                                     tGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCg                                   <  1:1950584/140‑1 (MQ=255)

                                                                                                     tGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCg                                   <  1:3734277/140‑1 (MQ=255)

                                                                                                     tGTACCCAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGTCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGCCCg                                   <  2:2324463/140‑1 (MQ=255)

                                                                                                      gTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCgg                                  <  2:4163560/140‑1 (MQ=255)

                                                                                                       tcgCGAAAATCGATAGCGGGGTATTGCTTATCCAGGGGCCTGCCAAACTCGCCGTTTTCATCGGTACGCGTTCGGCCGATATGGTTGTCAGCCCGGCCTTTAGCCAGCGGTCTGCCGTCTTCCGGCCGGATACGTGCGga                                 <  2:3527237/138‑1 (MQ=255)

                                                                                                       tAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGga                                 <  1:2612278/140‑1 (MQ=255)

                                                                                                       tAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGga                                 <  2:1741240/140‑1 (MQ=255)

                                                                                                         gCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTGCATCAGTTCGGGATCGTCCGATGTGGTTCGTAATCCGTGCGTTAGCCAGCAGTGTGTCGTCGACCGTACGGATACGTCCGcaga                               <  1:4196901/140‑1 (MQ=255)

                                                                                                              aaTCGATAGTGGGGTATTTCTTATCCATGTCCATGCCAAACTCGCCTTTTTCATCGGTTCGGGTTCGGCCGATATCGTTGTTACTCCGTGCGTCTGACAGCAGTGTGCCGTCTTCCGCACGGATCAGCCCGGAGAGGGtg                          >  2:4836330/1‑139 (MQ=255)

                                                                                                               aTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTaa                         >  1:3694494/1‑140 (MQ=255)

                                                                                                                  gATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCa                      <  2:817873/140‑1 (MQ=255)

                                                                                                                   aTAGTGGGGTATTTCTTATCCATGTCCATGACAATCTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCAt                     <  1:1267661/140‑1 (MQ=255)

                                                                                                                           gTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCa             >  2:676992/1‑140 (MQ=255)

                                                                                                                            tATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCAc            >  2:4653796/1‑140 (MQ=255)

                                                                                                                              tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct          <  1:858276/140‑1 (MQ=255)

                                                                                                                              tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct          <  1:4348621/140‑1 (MQ=255)

                                                                                                                                tCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACctct        <  1:3229736/140‑1 (MQ=255)

                                                                                                                                tCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACctct        <  2:1163565/140‑1 (MQ=255)

                                                                                                                                tCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACctct        <  1:3143306/140‑1 (MQ=255)

                                                                                                                                    aaccATGTGCATGTCAAACTCTCCGCGCTGATCGGTTCGGGTTCGGCCGCTATGGTCGTTAAGACATGGGTTCGCCAGCAGTGGGCCGCGTTCCGCCCGGATACGGCTGGAGACGGTGCCCATCTACTTCACctcccgct    >  2:946048/3‑135 (MQ=255)

                                                                                                                                    aTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCt    >  2:1584755/1‑140 (MQ=255)

                                                                                                                                    aTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCt    >  1:1692830/1‑140 (MQ=255)

CGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCA  >  NZ_CP025268/305892‑306165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`