BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,913,061	T→C	112 (TAA→TAG)	mazF ←	endoribonuclease MazF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,913,061	0	T	C	99.7%	1007.4 / NA	297	112 (TAA→TAG)	mazF	endoribonuclease MazF
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (196/100); minor base G (1/0); total (197/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

ACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCT  >  NZ_CP025268/2912924‑2913197

aCGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTa                                                                                                                                        >  1:3026093/1‑140 (MQ=255)

 cGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAc                                                                                                                                       <  1:4680336/140‑1 (MQ=255)

  gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAcc                                                                                                                                      >  1:4321816/1‑140 (MQ=255)

  gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAcc                                                                                                                                      >  1:1221123/1‑140 (MQ=255)

    gCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCa                                                                                                                                    >  1:3191762/1‑140 (MQ=255)

       gCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATc                                                                                                                                 <  1:2687055/140‑1 (MQ=255)

                    tCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAAttt                                                                                                                    <  1:1000165/140‑1 (MQ=255)

                     cgcgCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAAtttt                                                                                                                   >  1:554244/1‑140 (MQ=255)

                       cgcAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTgg                                                                                                                 >  1:3513290/1‑140 (MQ=255)

                       cgcAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTgg                                                                                                                 >  2:2756192/1‑140 (MQ=255)

                               cTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                         >  2:2366965/1‑140 (MQ=255)

                               cTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                         <  1:750440/140‑1 (MQ=255)

                                 gCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAg                                                                                                       <  1:4897230/140‑1 (MQ=255)

                                       taGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGTATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTaa                                                                                                 <  1:2588433/140‑1 (MQ=255)

                                       taGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTaa                                                                                                 <  2:4006368/140‑1 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTc                                                                                              >  2:5232838/1‑140 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTc                                                                                              >  1:3670637/1‑140 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTc                                                                                              >  2:3792498/1‑140 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTc                                                                                              >  2:4522836/1‑140 (MQ=255)

                                          tGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTc                                                                                              >  2:1038231/1‑140 (MQ=255)

                                          tGAGCAAACGGGCAATTTGATTCAATGAATGGGTCTGAAAATGGCAGGTCTGTCAGGTGTAAACCTGTGTCCAGAATACAAGTGAGTTAGTAACAGTACCCAATCAGTACGTTAATTTTGGCTTTAATGGGTTAGAATTc                                                                                              >  2:1741114/1‑140 (MQ=255)

                                            aGCAGACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTGATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:4912981/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGGATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:4792115/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAAGATGTGACCAGAATAGAAGTGAGGTAGTAACACTACCCAATCAGAACGTTAATTTCGGCTTTAATGAGTTGTAAtcact                                                                                            >  2:4322970/1‑136 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                         <  2:349205/127‑1 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  1:2875664/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  1:3746401/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:5093662/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:2393410/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:281758/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  1:2376550/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:426244/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:3841807/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGATAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:5139574/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGAGTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATAAGTACGTTAATGTTGGGTTGAATGAGTGGTAATTCct                                                                                            >  2:899532/1‑140 (MQ=255)

                                            aGCAAACGGACGATTTGATTCATTGAATGGTCCTGAAAATTGCGGGTCTGGCAGGTGGAAACCTTTGACCAGAAAAGAAGTGAGTTAGTTAGACTACCCAATCAGTACGTTAAATTTGGCTGTAATGCGTTGTAATTGct                                                                                            >  2:1999609/1‑140 (MQ=255)

                                              cAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATTAGTTGTAATTCctct                                                                                          >  1:1168307/1‑140 (MQ=255)

                                                 aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTggg                                                                                       >  1:1810449/1‑140 (MQ=255)

                                                   ggTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGc                                                                                     >  1:3428594/1‑140 (MQ=255)

                                                      cGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGATAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAAc                                                                                  >  2:2099355/1‑140 (MQ=255)

                                                        aTTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTg                                                                                >  1:495422/1‑140 (MQ=255)

                                                            gATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATAAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTcc                                                                            <  1:1600301/140‑1 (MQ=255)

                                                                       ttGTCGTGAAAATTGCGGGTCTGTCAGGGGGAAACTTGTGACCAGAAGAGACATGAGTTAGCAATACTGCGCAATCTGTACGTTAATTTTGGCTTGAATGAGGTGTAATTCCGCTGGGGCAACTGTTCCTTTCTGTGTTg                                                                 <  2:690996/140‑1 (MQ=255)

                                                                       ttGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTg                                                                 <  2:1199822/140‑1 (MQ=255)

                                                                       tcctCCTGAGATTTGCGGGTCTGTCCGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTCGCAACACTCCCCAATGAGTCCGTTAATTTTCGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTg                                                                 <  2:2790108/137‑1 (MQ=255)

                                                                           ccTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAAGGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGctcc                                                             >  1:2781757/1‑140 (MQ=255)

                                                                            cTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGctcct                                                            >  1:960756/1‑140 (MQ=255)

                                                                               aaaaTTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCtt                                                         >  1:1342719/1‑140 (MQ=255)

                                                                                  aTTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGcc                                                      <  2:142524/140‑1 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTCATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  2:2919795/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  1:2183187/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  1:3530669/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    <  1:4386773/140‑1 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    <  2:3943509/140‑1 (MQ=255)

                                                                                    tGCGGGGCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  1:4075468/1‑140 (MQ=255)

                                                                                         gTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAgg                                               >  1:2531912/1‑140 (MQ=255)

                                                                                            tttCAGGCGGAACCCTGTGACCAGAATAGAAGTGAGTTCGTACCACTACCCAATCAGTCCGTTAATTCTGGATTTACTGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTCGCTCCTCTTGCCCGACAGGCGa                                            <  2:4050234/138‑1 (MQ=255)

                                                                                            tGTCAGGTGGCACCCTGTCACCAGGATAGCAGTGAGTTAGTAACCCCCCCCAATCAGTCCGTTAATTTTGGCTTTAATGATTTGTAATTCCTCTGGGGCATCTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                            <  2:1544824/140‑1 (MQ=255)

                                                                                            tGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                            <  1:4213316/140‑1 (MQ=255)

                                                                                            tGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                            <  2:4033061/140‑1 (MQ=255)

                                                                                              tCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATa                                          >  2:4627105/1‑140 (MQ=255)

                                                                                                 ggTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACtt                                       <  1:3636349/140‑1 (MQ=255)

                                                                                                   tGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACtttt                                     >  1:3591509/1‑140 (MQ=255)

                                                                                                   tGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACtttt                                     <  1:2366965/140‑1 (MQ=255)

                                                                                                      acaCCTGTGACCAGAATAGAAGTGACGTAGTAACTCGACCCGATCAGTACGTTACTTTTGGATTTAATCAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGATCCTCTTGCCCGCCAGGCGATACTTTTTAc                                  <  2:3533284/138‑1 (MQ=255)

                                                                                                       aaCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTAcc                                 <  1:4591902/140‑1 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCATCTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  2:2967017/140‑1 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  2:4829820/140‑1 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  2:2664936/140‑1 (MQ=255)

                                                                                                           tgtgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGAt                             <  2:413548/140‑1 (MQ=255)

                                                                                                            gtgACAAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATc                            >  2:3130369/1‑140 (MQ=255)

                                                                                                             tgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCa                           >  2:777110/1‑140 (MQ=255)

                                                                                                             tgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCa                           >  1:2831634/1‑140 (MQ=255)

                                                                                                               aCCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGc                         >  2:1944603/1‑140 (MQ=255)

                                                                                                                ccAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCt                        <  1:5124769/140‑1 (MQ=255)

                                                                                                                    aaTAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACg                    >  1:1428065/1‑140 (MQ=255)

                                                                                                                    aaTAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACg                    >  1:3995480/1‑140 (MQ=255)

                                                                                                                      tAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCt                  <  1:1969531/140‑1 (MQ=255)

                                                                                                                         aaGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACg               >  2:954208/1‑140 (MQ=255)

                                                                                                                          aGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGc              <  2:3464247/140‑1 (MQ=255)

                                                                                                                              atttAGTAACACTACCCAATCAGTACGTTAATTTTGGCTGTAATGAGTTGGAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGGCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:3288347/3‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTa                                                                                                            >  2:5320100/1‑42 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTg                                                                 <  2:2457421/85‑1 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:4831929/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:105020/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:1892167/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:2265948/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:5225668/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:2764716/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:3341372/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:4974805/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:5195505/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:1670992/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:4768905/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:2279627/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:346129/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:3570398/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGGTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCGATGCGATACTTTTTACCTGATCAGCTAACGCTAAGCCATc          >  2:3724393/1‑140 (MQ=255)

                                                                                                                                  aGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGt      >  1:1745189/1‑140 (MQ=255)

                                                                                                                                   gTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGtt     >  1:2019653/1‑140 (MQ=255)

ACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCT  >  NZ_CP025268/2912924‑2913197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`