BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,913,722	T→C	745 (TAA→TAG)	relA ←	GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,913,722	0	T	C	99.5%	1386.9 / NA	408	745 (TAA→TAG)	relA	GTP pyrophosphokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (331/75); minor base G (2/0); total (333/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 408 total aligned reads displayed.

GTAGCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGTGTCGCTA  >  NZ_CP025268/2913586‑2913857

gTAGCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTaa                                                                                                                                      <  2:2165439/140‑1 (MQ=255)

   gCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTc                                                                                                                                   >  1:1094826/1‑140 (MQ=255)

   gCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTc                                                                                                                                   >  1:3412263/1‑140 (MQ=255)

   gCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTc                                                                                                                                   >  2:5152911/1‑140 (MQ=255)

    ccGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTcc                                                                                                                                  >  1:5208295/1‑140 (MQ=255)

              cACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCg                                                                                                                        >  1:1482967/1‑140 (MQ=255)

              cACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCg                                                                                                                        >  1:2599687/1‑140 (MQ=255)

                  gccgGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCACACCCCTATCATATGTAGATACAGCATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGCGCAACCGAcgc                                                                                                                    >  1:1290210/1‑140 (MQ=255)

                  gccgGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAACCCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGAcgc                                                                                                                    <  2:5207975/140‑1 (MQ=255)

                   ccgGTGAATTTCCCCAACGCTTTACGCTACTGTGGGTCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATGGTAGATACGAGCAAATTTCTGCCTAACTCCCGTGCAACCGAcgcg                                                                                                                   >  2:1682748/1‑140 (MQ=255)

                              ccccAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACa                                                                                                        >  2:2463017/1‑140 (MQ=255)

                                    cGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGc                                                                                                  >  1:3266321/1‑140 (MQ=255)

                                                cTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGt                                                                                      >  1:2344034/1‑140 (MQ=255)

                                                     gATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTAcc                                                                                 >  1:1327641/1‑140 (MQ=255)

                                                     caTCATAACCCTTTCGTCAAACCGCTATCATATGTAGATAAAGTATATATCAATCTACATTGTAGATACGCGCAAATTTCCGCCTCACTCCCCTGCACCCGACGCGCGTCGATCACATCCGGCACCTGCATGACTTTAcc                                                                                 >  2:4119258/2‑140 (MQ=255)

                                                      aTCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACAGTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTAccc                                                                                >  2:3302894/1‑140 (MQ=255)

                                                          tAACCCTTTCCTCAAACCGCTATCATATGTCGATACCGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                            >  2:1368832/1‑140 (MQ=255)

                                                          tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCg                                                                                                    <  2:3859943/116‑1 (MQ=255)

                                                          tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                            >  2:4727836/1‑140 (MQ=255)

                                                          tAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCa                                                                            >  2:4980014/1‑140 (MQ=255)

                                                            aCCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCAcg                                                                          <  1:92385/140‑1 (MQ=255)

                                                                tttCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcc                                                                      >  1:3842194/1‑140 (MQ=255)

                                                                tttCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcc                                                                      >  2:2760745/1‑140 (MQ=255)

                                                                 ttCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGccc                                                                     >  1:3037773/1‑140 (MQ=255)

                                                                 ttCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGccc                                                                     >  2:3513828/1‑140 (MQ=255)

                                                                  tCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcccc                                                                    <  2:4233203/140‑1 (MQ=255)

                                                                  tCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGcccc                                                                    >  2:1218255/1‑140 (MQ=255)

                                                                  tCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTACGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCCACTGGTTGAGTTTACCCAGCACGCGcccc                                                                    >  2:112029/1‑140 (MQ=255)

                                                                    cTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAg                                                                  <  1:3556151/140‑1 (MQ=255)

                                                                    cTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAg                                                                  <  2:2554206/140‑1 (MQ=255)

                                                                       aaaCCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCAc                                                               >  1:252338/1‑140 (MQ=255)

                                                                        aaCCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACt                                                              >  1:380177/1‑140 (MQ=255)

                                                                        aaCCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACt                                                              <  2:2844665/140‑1 (MQ=255)

                                                                            gCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGCCGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCa                                                          <  2:3775968/140‑1 (MQ=255)

                                                                              tATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAgg                                                        >  2:2258895/1‑140 (MQ=255)

                                                                                     tGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTaaa                                                 >  1:4143494/1‑140 (MQ=255)

                                                                                          aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                            >  2:187840/1‑140 (MQ=255)

                                                                                          aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                            >  1:4994171/1‑140 (MQ=255)

                                                                                          aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                            >  2:4889123/1‑140 (MQ=255)

                                                                                          aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                            >  2:4319152/1‑140 (MQ=255)

                                                                                          aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                            >  2:420511/1‑140 (MQ=255)

                                                                                          aTACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCa                                            >  2:3977313/1‑140 (MQ=255)

                                                                                            aCAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGt                                                                                           >  2:1723387/1‑91 (MQ=255)

                                                                                            aCAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAAt                                          >  2:1032363/1‑140 (MQ=255)

                                                                                              aGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATgg                                        >  2:3614050/1‑140 (MQ=255)

                                                                                              aGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATgg                                        >  1:3112516/1‑140 (MQ=255)

                                                                                                tatataTCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCGAGCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATggac                                      >  2:3190618/1‑138 (MQ=255)

                                                                                                 atatatCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCa                                     >  1:724900/1‑140 (MQ=255)

                                                                                                 atatatCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCa                                     >  2:4420218/1‑140 (MQ=255)

                                                                                                     atCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGt                                                                                           >  2:729094/1‑82 (MQ=255)

                                                                                                     atCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAAt                                                <  1:2680613/125‑1 (MQ=255)

                                                                                                     atCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTc                                 >  1:2822262/1‑140 (MQ=255)

                                                                                                     atCAATCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTc                                 >  2:3992375/1‑140 (MQ=255)

                                                                                                          tgtACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGATTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCGATGGTCATGTCGATgg                            >  1:4272594/3‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  1:4653191/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  1:2767003/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  2:333089/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  1:2888311/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  1:4771082/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  2:3173066/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  2:3097001/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  1:842400/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  1:2456104/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  1:3637600/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  1:2099114/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  1:4702916/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  2:4583100/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  1:167500/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  2:1584479/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  1:150781/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGATTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  1:1174027/1‑140 (MQ=255)

                                                                                                          tCTACATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGGCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATgg                            >  2:680476/1‑140 (MQ=255)

                                                                                                              cATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGc                        >  1:1569733/1‑140 (MQ=255)

                                                                                                              cATTGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGCCGGCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATGTCAATGGTCTTGCCGATGGTCGc                        <  2:3178941/140‑1 (MQ=255)

                                                                                                                 tGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAg                     >  1:4536967/1‑140 (MQ=255)

                                                                                                                 tGTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTAGCCAg                     >  2:2203721/1‑140 (MQ=255)

                                                                                                                  gTAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGt                    <  2:860163/140‑1 (MQ=255)

                                                                                                                   tAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGtt                   >  2:3586507/1‑140 (MQ=255)

                                                                                                                   tAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGtt                   >  1:1825356/1‑140 (MQ=255)

                                                                                                                   tAGATACGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGtt                   <  1:1942152/140‑1 (MQ=255)

                                                                                                                        aCGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGt                                       <  2:818791/115‑1 (MQ=255)

                                                                                                                        aCGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGt              >  2:4379818/1‑140 (MQ=255)

                                                                                                                        aCGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGt              >  1:4420456/1‑140 (MQ=255)

                                                                                                                        aCGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGt              >  1:3705698/1‑140 (MQ=255)

                                                                                                                        aCGAGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCGGt              >  1:4088883/1‑140 (MQ=255)

                                                                                                                           aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg           >  1:2163873/1‑140 (MQ=255)

                                                                                                                           aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg           >  1:3883871/1‑140 (MQ=255)

                                                                                                                           aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg           >  2:4447817/1‑140 (MQ=255)

                                                                                                                           aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg           >  1:5145673/1‑140 (MQ=255)

                                                                                                                           aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg           >  1:4011553/1‑140 (MQ=255)

                                                                                                                           aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg           >  1:5098114/1‑140 (MQ=255)

                                                                                                                           aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg           >  2:128657/1‑140 (MQ=255)

                                                                                                                           aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGGTTg           >  2:574518/1‑140 (MQ=255)

                                                                                                                           aGCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACAGCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTg           >  2:2957872/1‑140 (MQ=255)

                                                                                                                            gCAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTGGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTgg          <  1:3185422/140‑1 (MQ=255)

                                                                                                                             cAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGgt         <  1:1378826/140‑1 (MQ=255)

                                                                                                                             cAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGgt         >  1:1027268/1‑140 (MQ=255)

                                                                                                                             cAAATTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGgt         >  2:264508/1‑140 (MQ=255)

                                                                                                                                aTTTCGGCCTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGTGTc      >  1:430343/1‑140 (MQ=255)

                                                                                                                                   tCGGCCTAACGCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGTGTCGCt   <  2:453128/140‑1 (MQ=255)

GTAGCCGGGATCCGCACCGCCGGTGAATTTCCCCAACGCTTTACGCTACTGTGGATCATAACCCTTTCCTCAAACCGCTATCATATGTAGATACAGTATATATCAATCTACATTGTAGATACGAGCAAATTTCGGCTTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCTGGTTGAGTTTACCCAGCACGCGCCCCAGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGTGTCGCTA  >  NZ_CP025268/2913586‑2913857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`