BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,960,614	C→T	A192A (GCG→GCA)	ptrA ←	pitrilysin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,960,614	0	C	T	99.0%	1150.9 / NA	309	A192A (GCG→GCA)	ptrA	pitrilysin
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (136/170); minor base G (3/0); total (139/170)
Fisher's exact test for biased strand distribution p-value = 8.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

TTACTGTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCAT  >  NZ_CP025268/2960477‑2960750

ttaCTGTAAATACCCGCCGTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCGGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTgtgc                                                                                                                                        <  2:4772412/140‑1 (MQ=255)

     gTAAATAGCGGCCTTCGTCAAATGGGCGGGGTAGGACGTCTGGTGGAAGTCTTTGAGCGCTTGCGGCACCGGATGACCAGGTTTGTCGCTTAAAGTTGCGGGGGTTCCACCTGAAGAGTTGGAACCGGGGGGTGCCGGGt                                                                                                                                   >  2:325178/1‑140 (MQ=255)

     gTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGt                                                                                                                                   >  1:4366788/1‑140 (MQ=255)

      tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  1:711435/1‑140 (MQ=255)

      tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  1:3748932/1‑140 (MQ=255)

        aaTAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTaa                                                                                                                                >  2:4363896/1‑140 (MQ=255)

        aaTAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTATCGCTTAAAGTTTCGAGGTTACCACCAGAAAATTTTGAACCGGGGTGTGCCGGGTTaa                                                                                                                                >  2:4835429/1‑140 (MQ=255)

         ctacctgCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAAt                                                                                                                               <  2:5180538/134‑1 (MQ=255)

              cGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  1:3459010/140‑1 (MQ=255)

              cGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  2:2610774/140‑1 (MQ=255)

              cGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  1:4012428/140‑1 (MQ=255)

              cGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGCATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  1:4719526/140‑1 (MQ=255)

              cGCCCTCATCACATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  2:5236599/140‑1 (MQ=255)

                ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGTGGTGTGCCGGGTTAATGGTTTCt                                                                                                                        >  1:1151351/1‑140 (MQ=255)

                ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCt                                                                                                                        >  2:13332/1‑140 (MQ=255)

                  ttCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGTGGTGTGCCGGGTTAATGGTTTCTgc                                                                                                                      >  2:687875/1‑140 (MQ=255)

                  ttCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgc                                                                                                                      >  1:2471851/1‑140 (MQ=255)

                  ttCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgc                                                                                                                      >  2:762084/1‑140 (MQ=255)

                   tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  2:4733183/140‑1 (MQ=255)

                   tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  1:924081/140‑1 (MQ=255)

                   tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  1:2818614/140‑1 (MQ=255)

                   tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  2:1587441/140‑1 (MQ=255)

                   tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  2:3676210/140‑1 (MQ=255)

                   tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  2:3289264/140‑1 (MQ=255)

                   tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  1:5038122/140‑1 (MQ=255)

                     atcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCt                                                                                                                   <  2:1815102/140‑1 (MQ=255)

                        aaaTTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGAc                                                                                                                <  1:3321597/140‑1 (MQ=255)

                         aaTTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGAcc                                                                                                               <  1:1298996/140‑1 (MQ=255)

                          aTTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCt                                                                                                              <  1:516332/140‑1 (MQ=255)

                           ttGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCtg                                                                                                             >  1:3250333/1‑140 (MQ=255)

                           ttGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCtg                                                                                                             >  1:1865619/1‑140 (MQ=255)

                              gCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGc                                                                                                          <  1:4688866/140‑1 (MQ=255)

                               cGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGcc                                                                                                         >  1:1360490/1‑140 (MQ=255)

                               cGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGcc                                                                                                         <  2:3943680/140‑1 (MQ=255)

                                        aCTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATc                                                                                                >  2:4553929/1‑140 (MQ=255)

                                         cTTCTCGTGGAAATAGTTCTGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAGGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATcc                                                                                               >  2:1017113/1‑140 (MQ=255)

                                            ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCGGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                            >  1:4880766/1‑140 (MQ=255)

                                            ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATTCCGt                                                                                            <  2:4910694/140‑1 (MQ=255)

                                            ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                            >  1:4312210/1‑140 (MQ=255)

                                            ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                            >  1:2147971/1‑140 (MQ=255)

                                            ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                            >  1:1238329/1‑140 (MQ=255)

                                              cGTGGAAATCTTTCAGCGCCTGCTGCCCCGGATTACAAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCTCGTCa                                                                                          >  2:144553/1‑140 (MQ=255)

                                              cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTACAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGTCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCa                                                                                          >  1:3186734/1‑140 (MQ=255)

                                              cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCa                                                                                          >  2:746987/1‑140 (MQ=255)

                                               gTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTGTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCGGGGCTAATGGATTCTGCGCTGACCTTTGCCATGCGCATCCCGTCAc                                                                                         >  2:679653/1‑140 (MQ=255)

                                                 ggAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCAcgc                                                                                       >  1:3308537/1‑140 (MQ=255)

                                                 ggAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCAcgc                                                                                       >  1:3059572/1‑140 (MQ=255)

                                                   aaaTCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGt                                                                                     <  1:76963/140‑1 (MQ=255)

                                                        tttCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTAcgcg                                                                                <  2:1398899/140‑1 (MQ=255)

                                                        tttCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTAcgcg                                                                                <  1:5230827/140‑1 (MQ=255)

                                                         ttCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACgcgc                                                                               >  1:1402205/1‑140 (MQ=255)

                                                          tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              <  2:2244618/140‑1 (MQ=255)

                                                          tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              <  1:3019417/140‑1 (MQ=255)

                                                          tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              <  2:4084921/140‑1 (MQ=255)

                                                          tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              <  1:3555190/140‑1 (MQ=255)

                                                          tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              <  2:2471851/140‑1 (MQ=255)

                                                          tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              <  2:4417591/140‑1 (MQ=255)

                                                                ccTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGtt                                                                        >  1:2642442/1‑140 (MQ=255)

                                                                   gctgcACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAAt                                                                     <  1:4199183/140‑1 (MQ=255)

                                                                      gcACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCa                                                                  <  1:440554/140‑1 (MQ=255)

                                                                      gcACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCa                                                                  <  2:2652173/140‑1 (MQ=255)

                                                                           ggATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGtt                                                             <  1:3368201/140‑1 (MQ=255)

                                                                           ggATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCCTACGCGCCATGGTTAATTCAGCGtt                                                             <  1:4529564/140‑1 (MQ=255)

                                                                             aTTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCa                                                           <  1:38320/140‑1 (MQ=255)

                                                                             aTTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCa                                                           <  1:1803971/140‑1 (MQ=255)

                                                                             aTTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCa                                                           <  1:495000/140‑1 (MQ=255)

                                                                                 ccAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGc                                                       >  1:3647151/1‑140 (MQ=255)

                                                                                 ccAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGc                                                       >  1:1898171/1‑140 (MQ=255)

                                                                                 ccAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGc                                                       >  2:555815/1‑140 (MQ=255)

                                                                                 ccAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGGTCACCGc                                                       >  2:288437/1‑140 (MQ=255)

                                                                                  cAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCa                                                      <  2:5154578/140‑1 (MQ=255)

                                                                                  cAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCa                                                      <  1:1586430/140‑1 (MQ=255)

                                                                                   aGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCAt                                                     <  2:3148333/140‑1 (MQ=255)

                                                                                   aGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCAt                                                     <  2:843651/140‑1 (MQ=255)

                                                                                     gTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTa                                                   <  1:5350165/140‑1 (MQ=255)

                                                                                     gTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTa                                                   <  1:5103098/140‑1 (MQ=255)

                                                                                      tttGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGGCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGGTAATTCAGCGTTCACCGCATTAc                                                  >  2:2982912/1‑140 (MQ=255)

                                                                                      tttGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTa                                                                                    <  1:1659429/106‑1 (MQ=255)

                                                                                      tttGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTa                                                                                    >  2:1659429/1‑106 (MQ=255)

                                                                                       ttGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACg                                                 <  2:3353558/140‑1 (MQ=255)

                                                                                        tGTCGCTTAAAGTTTCGAGGTTACGACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTa                                                                                    >  2:1520918/1‑104 (MQ=255)

                                                                                        tGTCGCTTAAAGTTTCGAGGTTACCGCCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTa                                                                                    <  1:1520918/104‑1 (MQ=255)

                                                                                         gTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCt                                               >  1:1365823/1‑140 (MQ=255)

                                                                                         gTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCt                                               >  1:2723067/1‑140 (MQ=255)

                                                                                              ttAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGt                                          >  1:4894234/1‑140 (MQ=255)

                                                                                               tagaGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGGACGCGCCATGGTTAATTCAGCGTTCACCGCAGTACGCTCACGtt                                         <  1:2382666/137‑1 (MQ=255)

                                                                                               tAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATTGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGGTCACCGCATTACGCTCACGtt                                         <  1:3523193/140‑1 (MQ=255)

                                                                                               tAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGtt                                         <  1:103472/140‑1 (MQ=255)

                                                                                               tAAAGTGTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGtt                                         >  1:2929624/1‑140 (MQ=255)

                                                                                                   gTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGc                                     <  2:621801/140‑1 (MQ=255)

                                                                                                      tCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCata                                  >  1:3082722/1‑140 (MQ=255)

                                                                                                              accaccAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGt                          >  2:4500073/1‑140 (MQ=255)

                                                                                                              accaccAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCACGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGt                          >  2:3737541/1‑140 (MQ=255)

                                                                                                                   cacaacccTTTGAACCGCGGCGTGCGGCGTTACTGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCCGCACATTTCTTGTCGAGc                     <  2:3539684/133‑1 (MQ=255)

                                                                                                                    aGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCa                    <  2:2375346/140‑1 (MQ=255)

                                                                                                                        aaCTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAgg                <  2:1127920/140‑1 (MQ=255)

                                                                                                                                    gggTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGc    >  1:839105/1‑140 (MQ=255)

                                                                                                                                     ggTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCa   <  1:2024361/140‑1 (MQ=255)

                                                                                                                                      gtgtGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCAt  <  1:2549398/140‑1 (MQ=255)

                                                                                                                                      gtgtGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCAt  <  2:3241381/140‑1 (MQ=255)

TTACTGTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCAT  >  NZ_CP025268/2960477‑2960750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`