BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,085,924	A→G	C60R (TGT→CGT)	speB ←	agmatinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,085,924	0	A	G	99.7%	1154.1 / NA	339	C60R (TGT→CGT)	speB	agmatinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (155/183); minor base T (0/1); total (155/184)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

AGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAGGCATT  >  NZ_CP025268/3085788‑3086059

aGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGAt                                                                                                                                      <  2:431416/140‑1 (MQ=255)

aGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGAt                                                                                                                                      <  1:71989/140‑1 (MQ=255)

aGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGAt                                                                                                                                      <  2:3332325/140‑1 (MQ=255)

          tgacctcACGGGCATCCCCAACGTCATATACCAGATCGCCGAAGACCACGACGTTCAGACGCTTACGCATGTCGAACATCCACCGGAAGCGCTTGTGTTCCCAGGCCAGATCCGTCGAAACCTGACGGATCGCTGCCGGa                                                                                                                            <  2:4465331/136‑1 (MQ=255)

            aTCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGAcc                                                                                                                          >  2:2084600/1‑140 (MQ=255)

                 aCGGGCATCGCCAAAGTCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGc                                                                                                                     <  2:1595603/140‑1 (MQ=255)

                      cATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGacca                                                                                                                <  1:1431440/140‑1 (MQ=255)

                        tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGaccacc                                                                                                              >  1:809411/1‑140 (MQ=255)

                        tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGaccacc                                                                                                              >  2:2636714/1‑140 (MQ=255)

                        tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGaccacc                                                                                                              >  2:5332003/1‑140 (MQ=255)

                        tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGaccacc                                                                                                              >  1:4673602/1‑140 (MQ=255)

                        tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTCTGTTCCCAGGCCAGATTCGTCGTAACCTGACGGATCGCTGCCAGACCGTGGCGaccacc                                                                                                              >  1:2978338/1‑140 (MQ=255)

                        tCGCCAAAGGCATATACCAGATCGCCGCAGTCCAAGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGacgacc                                                                                                              >  2:4170920/1‑140 (MQ=255)

                        tCGCCAAAGGCATAAACCAGATCGCCGCAGTCCACGATGTTCAGACGCTAACGCATGTCAAAATTACACGGGAAGCGGTTGTGTTCCCAGGCCAGATTAGTCTAAACCTGACGGATACCTGCCGGGACGAGGAGaccacc                                                                                                              >  2:647156/1‑140 (MQ=255)

                           ccAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGc                                                                                                           <  1:1941545/140‑1 (MQ=255)

                           ccAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGc                                                                                                           <  2:3935968/140‑1 (MQ=255)

                           ccAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGc                                                                                                           <  1:1364495/140‑1 (MQ=255)

                           ccAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGc                                                                                                           <  1:3408389/140‑1 (MQ=255)

                           ccAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGc                                                                                                           <  2:3530606/140‑1 (MQ=255)

                                ggCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGAc                                                                                                      <  2:1619708/140‑1 (MQ=255)

                                   atatACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAg                                                                                                   >  1:432778/1‑140 (MQ=255)

                                    tataCCAGATCGCCGCAGTCCCCGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGa                                                                                                  <  2:4144841/140‑1 (MQ=255)

                                    tataCCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTGCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGa                                                                                                  >  2:2967587/1‑140 (MQ=255)

                                    tataCCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGa                                                                                                  >  2:2557317/1‑140 (MQ=255)

                                    tataCCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGa                                                                                                  >  2:3760566/1‑140 (MQ=255)

                                     ataCCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGaa                                                                                                 <  1:1595665/140‑1 (MQ=255)

                                     ataCCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGCACCGTGGCGACCACCCGCACGACCAGaa                                                                                                 >  2:320586/1‑140 (MQ=255)

                                      taCCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAg                                                                                                >  2:1192503/1‑140 (MQ=255)

                                         cacaTCGCCGCAGTCCCCGACGTTCCAACACTCACGCATGTCGAAATCCCACCGGACGCGGTTGTGTTCCCCGGCCAGATTCGCCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTgg                                                                                             <  2:464361/137‑1 (MQ=255)

                                           gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGTAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGAGCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc                                                                                           >  2:3390990/1‑140 (MQ=255)

                                           gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc                                                                                           >  1:3726584/1‑140 (MQ=255)

                                           gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc                                                                                           >  1:1513021/1‑140 (MQ=255)

                                           gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc                                                                                           >  1:1562642/1‑140 (MQ=255)

                                           gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc                                                                                           >  2:3092759/1‑140 (MQ=255)

                                           gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc                                                                                           >  2:1428303/1‑140 (MQ=255)

                                           gATCGCCGCAGACCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc                                                                                           >  1:5233087/1‑140 (MQ=255)

                                                ccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGTCGACCACCCGCACGACCAGAAGTGGCCATATc                                                                                      >  2:203870/1‑140 (MQ=255)

                                                ccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATc                                                                                      >  2:798704/1‑140 (MQ=255)

                                                 cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCg                                                                                     >  1:2555618/1‑140 (MQ=255)

                                                 cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCg                                                                                     >  1:1156176/1‑140 (MQ=255)

                                                   cAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGaa                                                                                   <  1:2174245/140‑1 (MQ=255)

                                                   cAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGaa                                                                                   <  1:3215102/140‑1 (MQ=255)

                                                   cAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATGCGTCGAAACCTGACGGATCGCTGGCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGaa                                                                                   <  1:2692614/140‑1 (MQ=255)

                                                      tCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGTGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCGCCCGCATGACCAGAAGTGGCCATATCGAAcag                                                                                >  1:88979/1‑138 (MQ=255)

                                                      tCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGCCGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACgg                                                                                <  2:896450/140‑1 (MQ=255)

                                                      tCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACgg                                                                                <  1:447475/140‑1 (MQ=255)

                                                       ccACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGc                                                                               >  2:2165072/1‑140 (MQ=255)

                                                           gacgTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGc                                                                           >  2:2545177/1‑140 (MQ=255)

                                                           gacgTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGc                                                                           <  1:1828427/140‑1 (MQ=255)

                                                            ccgTTCAGGCGCTCACGCATGTCGAAATTCCGCGGGAAGAGGTTGGGTTCCCAGGCCAGATTCGTCGAAGCCGGACGGATCGCTGCCGGACCGTGGCGGCCACCCGCGCGACCAGAAGTGGCCATGTCGAGCGGCACGcc                                                                          <  2:4002489/139‑1 (MQ=255)

                                                            acgTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGCTCGCTGCCGGACCGTGGCGACCCCCCGCACGACCAGAAGTGGCCATATCGAACGGCACGcc                                                                          <  1:3841604/140‑1 (MQ=255)

                                                            acgTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGcc                                                                          <  2:3891564/140‑1 (MQ=255)

                                                            acgTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGcc                                                                          <  2:1656641/140‑1 (MQ=255)

                                                            acgTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGcc                                                                          <  2:715227/140‑1 (MQ=255)

                                                            acgTTCAGACGCGCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGcc                                                                          <  1:2847521/140‑1 (MQ=255)

                                                            acgTTCAGAAGCTCACGCTTGTCGAAGTTCCGCGGGGAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAGACCTGACGGATCTGTGCCGGACCGGGGGGACCACCCGCACGGCCAGAAGTGGCCATATCCAACGGCACGcc                                                                          <  2:3807371/140‑1 (MQ=255)

                                                             cgTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCa                                                                         >  1:4273276/1‑140 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCATGCCAGATGCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCAGGACCAGAAGTGGCCATATCGAACGGCACCCCAGTa                                                                      >  1:2346979/1‑140 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                      <  2:3053329/140‑1 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                      <  2:4788510/140‑1 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                      >  2:557742/1‑140 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                      >  2:1339491/1‑140 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                      <  2:2321241/140‑1 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                      >  1:1120752/1‑140 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                      >  1:2616749/1‑140 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                      >  1:3024339/1‑140 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                      >  1:418910/1‑140 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                      <  2:1042499/140‑1 (MQ=255)

                                                                     cGCTCACGCATGTCGAAATCCCACGGGGCGCGGTTGTGTTCGCAGGCCAGCTTCGTCGACACTTGACGGATCGCTGCCGGACCGTGGGGACCCCCCGCACGACCAGAAGTGGCCCTATCGACCGGCACGCCAGTAATCAc                                                                 <  2:1150466/140‑1 (MQ=255)

                                                                      gCTCCCGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAcc                                                                <  1:4815869/140‑1 (MQ=255)

                                                                      gCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAcc                                                                <  2:2433214/140‑1 (MQ=255)

                                                                      gCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAcc                                                                <  2:5080521/140‑1 (MQ=255)

                                                                       cTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGGCCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAccc                                                               >  2:4907894/1‑140 (MQ=255)

                                                                        tCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCa                                                              <  2:4425048/140‑1 (MQ=255)

                                                                         cACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAg                                                             >  2:5194572/1‑140 (MQ=255)

                                                                         cACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAg                                                             >  1:2260070/1‑140 (MQ=255)

                                                                              aTGTCGAAATTCCACGGGAAGCGGTTGGGTTCCAAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGc                                                        <  2:3693039/140‑1 (MQ=255)

                                                                               tGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGcc                                                                          <  2:2703387/121‑1 (MQ=255)

                                                                                gTCGAAATTCCCCGCGAAGCGGTTTTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCAt                                                      <  2:3385938/140‑1 (MQ=255)

                                                                                gTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATCTCGAACGGCACGCCAGTAATCACCCAGTCTGCAt                                                      <  1:1778022/140‑1 (MQ=255)

                                                                                gTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCAt                                                      <  1:2084600/140‑1 (MQ=255)

                                                                                gTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCAt                                                      <  1:3988318/140‑1 (MQ=255)

                                                                                  cGAAATTCCACGGGAACCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCg                                                    <  1:4360635/140‑1 (MQ=255)

                                                                                    aaaTTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCt                                                  >  1:5308085/1‑140 (MQ=255)

                                                                                                             cccAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGc                         <  1:4406334/140‑1 (MQ=255)

                                                                                                             cccAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTCGCGACCACCCGCACGCCCAGAAGTGGCCATATCGAACGGCAGGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCAGCGGc                         >  2:5016570/1‑140 (MQ=255)

                                                                                                                aGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGTGCGTGGCGACGACCCGCACGAGCAGAAGTGGCGATATCGAACGGCACGCCAGTAATCACGTAGTCTGCATCGCTGTCATATGGCTGGAAGTTGATCGGCAgg                      >  2:1838698/1‑140 (MQ=255)

                                                                                                                aGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAgg                      >  1:3599812/1‑140 (MQ=255)

                                                                                                                     aGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTaa                 <  2:406494/140‑1 (MQ=255)

                                                                                                                      gATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTaaa                <  1:3344936/140‑1 (MQ=255)

                                                                                                                       aTTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTaaaa               >  1:4767567/1‑140 (MQ=255)

                                                                                                                           gtcgAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAAcc           <  1:3482629/140‑1 (MQ=255)

                                                                                                                            tcgAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGAc                                                                                                      >  1:541362/1‑48 (MQ=255)

                                                                                                                            tcgAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCa                                                              <  2:211717/88‑1 (MQ=255)

                                                                                                                            tcgAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCa                                                              >  1:211800/1‑88 (MQ=255)

                                                                                                                            tcgAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCa          <  1:1033557/140‑1 (MQ=255)

                                                                                                                              gAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCaaa        <  1:3760566/140‑1 (MQ=255)

                                                                                                                                 agcTGACGGATCGCTGCCGGACCGTGGCGACCACCAGCACGGCGAGAAGTGGCGATATCGAGCGGCACTCCAGGAGTGACCCAGTATGCATCGGTGGCATAAGGCTGGGAGTTCATGGGGAGGGGGAAAAAAGCAAAggg     >  2:4495712/3‑139 (MQ=255)

                                                                                                                                  ccTAACTGATCGATGCCGGACCGTCGCGACCACACGTACGACCAGAAGTGCCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATGGCTGTCCTACGGCTGGAAGTTCATCGGCAGGCGGAAAAAACCAAAGGCa    <  2:1774210/140‑1 (MQ=255)

                                                                                                                                    tGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAGGCAtt  <  1:4845774/140‑1 (MQ=255)

AGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAGGCATT  >  NZ_CP025268/3085788‑3086059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`