BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,137,127	A→G	N231S (AAT→AGT)	yghT →	dTMP kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,137,127	0	A	G	99.7%	1108.8 / NA	331	N231S (AAT→AGT)	yghT	dTMP kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (154/176); minor base T (1/0); total (155/176)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 331 total aligned reads displayed.

AAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑G‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATGCT  >  NZ_CP025268/3136991‑3137262

       ccATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATg                                                                                                                                >  2:1558658/1‑140 (MQ=255)

          tAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACGGGAACAGGGGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGG‑GGGTATCACTAGTAAAAAATGGaa                                                                                                                             >  2:2407445/1‑140 (MQ=255)

            aCGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAAt                                                                                                                           >  1:2426429/1‑140 (MQ=255)

             cGCCACAGTTACCCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATg                                                                                                                          >  2:4029938/1‑140 (MQ=255)

               ccccccttAACCTTTGGTGGTGCACAACTGGTTGATATCGTTGCCAATCAGCCACTGGCACAGGTGTTGGTTGATCCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GCGGTTATCACTAGTCAAAAATGGAAATGcc                                                                                                                        <  2:4315539/134‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  1:1158832/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  1:5238023/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  2:3210522/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  1:1808566/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  2:4151283/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  1:2632731/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAAATGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  2:2289921/140‑1 (MQ=255)

                 acaGTTAAACTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  2:518939/140‑1 (MQ=255)

                 aaaGTTAACCTTGGGTGTGACACAACTGGTTGATATCGAGCCAAATCAGCCACTGGAAAGGGTGTTGGTTGATGAAAAAAAAGCGATTACGGTTTTTATGCCCGCGCGG‑GGTTATCACTAGTAAAAAATGGAAATCCCCg                                                                                                                      <  2:5345290/138‑1 (MQ=255)

                       aaCCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGcc                                                                                                                >  1:1050630/1‑140 (MQ=255)

                        aCCTTTGGTGGTGCACAACTGGTTGATATCGCTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCa                                                                                                               >  1:4774419/1‑140 (MQ=255)

                        aCCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCa                                                                                                               >  2:3313178/1‑140 (MQ=255)

                            ttGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                                           <  2:1838603/140‑1 (MQ=255)

                            ttGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                                           <  1:1215263/140‑1 (MQ=255)

                             tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAGAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                                          >  1:2772318/1‑140 (MQ=255)

                             tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                                          >  1:3897407/1‑140 (MQ=255)

                             tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                                          >  1:4404472/1‑140 (MQ=255)

                                    gCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                                   >  1:2911926/1‑140 (MQ=255)

                                    gCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                                   >  2:4840099/1‑140 (MQ=255)

                                    gCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                                   >  2:1155344/1‑140 (MQ=255)

                                         aCTGGTTGATCTCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATGTTCAg                                                                                              <  1:3911046/140‑1 (MQ=255)

                                         aCTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAg                                                                                              <  2:4694189/140‑1 (MQ=255)

                                          cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGG‑GGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                             >  1:5014118/1‑140 (MQ=255)

                                          cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                             >  1:2895664/1‑140 (MQ=255)

                                             gTTGATATCGCTGCCAATCATCCACGGGAACAGGTGTGTGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCTC‑GTGGTTATCACTAGTCAAAAGTGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAggcat                                                                                          >  2:5143374/1‑137 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                          >  1:698412/1‑140 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                          >  1:569378/1‑140 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                          >  2:685611/1‑140 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                          >  1:2434267/1‑140 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                          >  1:2309632/1‑140 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                          >  2:2196544/1‑140 (MQ=255)

                                               tGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGt                                                                                        >  1:5187576/1‑140 (MQ=255)

                                                    tCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAACTGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAAt                                                                                   <  2:2958658/140‑1 (MQ=255)

                                                     cGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                                  <  1:2019120/140‑1 (MQ=255)

                                                     cGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                                  <  2:825024/140‑1 (MQ=255)

                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                                >  1:4219398/1‑140 (MQ=255)

                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                                >  2:3376354/1‑140 (MQ=255)

                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                                >  2:4262665/1‑140 (MQ=255)

                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                                >  1:2987072/1‑140 (MQ=255)

                                                        tGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAAc                                                                               >  1:1600454/1‑140 (MQ=255)

                                                          ccAATCAGCCACTGTAACCGGTGTTGGTTGATGCGGAAAAAGCGGTTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAACTGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                             <  2:3606082/140‑1 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAACGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                             <  1:4909599/140‑1 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATTGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                             <  1:3194537/140‑1 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                             <  2:1061494/140‑1 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                             <  1:2346657/140‑1 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                             <  1:5349988/140‑1 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                             <  2:456769/140‑1 (MQ=255)

                                                            aaTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                                           <  1:4687973/140‑1 (MQ=255)

                                                            aaTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                                           <  2:441930/140‑1 (MQ=255)

                                                             aTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCaa                                                                          <  2:1461393/140‑1 (MQ=255)

                                                             aTCAGCCACTGGAACAGGTGTTGGTAGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCaa                                                                          >  2:4796660/1‑140 (MQ=255)

                                                               taGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GAGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:4717748/139‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  2:2844602/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  2:2675063/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:1125143/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:2671864/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:1412214/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:4560576/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:4600524/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:877581/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:689889/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:4948695/140‑1 (MQ=255)

                                                               cAGCCACTGGAAAAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCACGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:3103854/140‑1 (MQ=255)

                                                               cAGCCAATGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:1979059/140‑1 (MQ=255)

                                                                  ccccTGGAACGGGGGTTGGTTGATGCAGCAAAAGCGAGAACGGATCTTATGACCGCGC‑GTGGTTATCACTAGACGAAAATGGAAATGCCCGATGGCCAGGACCGTGCATATTCAGGAAGGGTAAATCAACTGCAAGGCTa                                                                     >  2:1912619/4‑140 (MQ=255)

                                                                  ccACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                                     >  1:1840161/1‑140 (MQ=255)

                                                                  ccACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTACCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                                     <  2:5237417/140‑1 (MQ=255)

                                                                      tGGAACAGGTGTTGGTTGATGCAGAAATAGCGGTTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCa                                                                 >  2:3937859/1‑140 (MQ=255)

                                                                      tGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCa                                                                 >  2:851292/1‑140 (MQ=255)

                                                                      tGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGACCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCa                                                                 >  1:3810825/1‑140 (MQ=255)

                                                                         aaCAGGTGTTGGTTGATGCAGAAAAATCGATTACGGATTTTATGACCGCACGG‑GGTTATCACTAGTCAAAAATGGAAAGCACCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATAAACTGCAATGCTATACAgga                                                              <  2:4624384/140‑3 (MQ=255)

                                                                         aaCAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTa                                                              <  2:3694273/140‑1 (MQ=255)

                                                                            aGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAg                                                           >  1:3371863/1‑140 (MQ=255)

                                                                                  tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                                                     >  2:57040/1‑140 (MQ=255)

                                                                                   ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacca                                                    >  2:3016058/1‑140 (MQ=255)

                                                                                        aTGCAGAAAAAGCGACAACGGATTGTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAGCGGGCATTTTCAGGAAGGCGAAATCAACTGCAATGCTATCCAGGACAGCCCACCagaaaa                                               >  2:5002916/1‑139 (MQ=255)

                                                                                                 aaGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGCTCGCCAGGACCGGGCATTTTCAGGAAGGTTAAACCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgc                                      <  2:1250004/140‑1 (MQ=255)

                                                                                                         aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                              <  1:3857200/140‑1 (MQ=255)

                                                                                                         aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                              <  2:390472/140‑1 (MQ=255)

                                                                                                         aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                              <  1:3436662/140‑1 (MQ=255)

                                                                                                         aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                              <  2:2476237/140‑1 (MQ=255)

                                                                                                         aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                              <  1:3038653/140‑1 (MQ=255)

                                                                                                         aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGATTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                              <  1:3663007/140‑1 (MQ=255)

                                                                                                          cGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCAGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa                             <  1:2153106/140‑1 (MQ=255)

                                                                                                                    gACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATAcc                   <  2:1934684/140‑1 (MQ=255)

                                                                                                                    gACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAAATGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATAcc                   <  2:351457/140‑1 (MQ=255)

                                                                                                                      ccGCGC‑GTGTTTATCACTAGTCAAAAATGGAAATCCCCCATCGCGACGACCGGGCATTTTAAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa                 <  2:1696943/140‑1 (MQ=255)

                                                                                                                      ccGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa                 <  1:1261431/140‑1 (MQ=255)

                                                                                                                      ccGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa                 >  2:2085978/1‑140 (MQ=255)

                                                                                                                       cgcgc‑gtGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCAc                <  2:3074192/140‑1 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc              <  1:2509115/135‑1 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc              >  1:4113795/6‑140 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc              >  2:1519672/6‑140 (MQ=255)

                                                                                                                            cgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCa            >  1:219502/4‑140 (MQ=255)

                                                                                                                                 ttATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCACCTGCAATGCTATCCAGTACAGCCCACCAGCAAGAAAAATTGCCGCCGGTAAAGTAAATACACACGCCATCAgg       >  2:932520/1‑140 (MQ=255)

AAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑G‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATGCT  >  NZ_CP025268/3136991‑3137262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`